急性冷胁迫对中华鳖幼鳖肠道黏膜组织的影响
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摘要
为了探究急性冷胁迫对中华鳖(Pelodiscus sinensis)幼鳖肠道不同区段黏膜组织学特征的影响,实验检测了急性冷胁迫前后血清二胺氧化酶(Diamine oxidase, DAO)的活性,同时观察了急性冷胁迫前后中华鳖肠道黏膜的相关组织形态的差异。DAO活性显示:(1)在第一次急性冷胁迫实验中,中华鳖血清DAO活性随急性冷胁迫时间的增加而呈现降低趋势,并在冷胁迫到达48h降到了最低水平;(2)在急性冷胁迫及复温实验中,中华鳖血清DAO的活性,在冷胁迫3d后显著降低,但随着温度的恢复, DAO活性又恢复到正常水平。组织病理结果显示:(1)急性冷胁迫对中华鳖肠道(回肠后段和大肠)黏膜上皮的形态没有明显影响;(2)急性冷胁迫对回肠后段的杯状细胞数目、肠绒毛长度和绒毛长度/隐窝深度的比值没有显著影响,但会使回肠后段黏膜厚度显著降低;(3)急性冷胁迫会使大肠的杯状细胞数目降低。这表明急性冷胁迫会改变中华鳖肠道黏膜的结构,但在不同的肠段,这种改变是不同的。回肠后段和大肠在同样的冷胁迫方式下黏膜机械屏障的不同变化情况,提示中华鳖肠道各段对急性冷胁迫具有特殊的应对方式。
To investigate the effects of acute cold stress on the mucosal histology in different sections of the juvenile Pelodiscus sinensis, the activity of serum diamine oxidase(DAO) and histological features of intestinal mucosa in Chinese soft-shelled turtle in culture temperature and acute cold stress were conducted. The first acute cold stress reduced the DAO activity with the lowest level at 48 h after cold stress. In the acute cold stress and rewarming experiment, the DAO activity decreased significantly after 3 days of cold stress and then restored to normal level with the recovery of temperature. Histopathological results showed no significant difference in the morphology of mucosal epithelium in the intestine(posterior segment of ileum and large intestine) between acute cold stress group and control group.There were no significant difference in the number of goblet cells, the length of villus and the ratio of villus length to crypt depth in the posterior segment of ileum between acute cold stress group and control group, while the mucosal thickness in the posterior segment of ileum significantly decreased in the acute cold stress group in comparison to control group. Acute cold stress significantly reduced the large intestine number of goblet cells. The structures of the intestinal mucosal mechanical barrier of Chinese soft-shelled turtle under acute cold stress are an intestinal segment dependent pattern. These results suggest that the various segments of the intestine of Chinese soft-shelled turtle have a special response style to acute cold stress.
To investigate the effects of acute cold stress on the mucosal histology in different sections of the juvenile Pelodiscus sinensis, the activity of serum diamine oxidase(DAO) and histological features of intestinal mucosa in Chinese soft-shelled turtle in culture temperature and acute cold stress were conducted. The first acute cold stress reduced the DAO activity with the lowest level at 48 h after cold stress. In the acute cold stress and rewarming experiment, the DAO activity decreased significantly after 3 days of cold stress and then restored to normal level with the recovery of temperature. Histopathological results showed no significant difference in the morphology of mucosal epithelium in the intestine(posterior segment of ileum and large intestine) between acute cold stress group and control group.There were no significant difference in the number of goblet cells, the length of villus and the ratio of villus length to crypt depth in the posterior segment of ileum between acute cold stress group and control group, while the mucosal thickness in the posterior segment of ileum significantly decreased in the acute cold stress group in comparison to control group. Acute cold stress significantly reduced the large intestine number of goblet cells. The structures of the intestinal mucosal mechanical barrier of Chinese soft-shelled turtle under acute cold stress are an intestinal segment dependent pattern. These results suggest that the various segments of the intestine of Chinese soft-shelled turtle have a special response style to acute cold stress.
引文
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[24]Zhang W.Effects of cold stress on chicken’s barrier function of intestinal mucosa[D].The Northeast Agricultural University.2010[张雯.冷应激对雏鸡肠黏膜屏障功能的影响.东北农业大学.2010]
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[2]Somfai-Relle S,Schauss A G,Financsek I,et al.Acute and subchronic toxicity studies of cryogenically-frozen,cryomilled,Pelodiscus sinensis(Japanese soft-shelled turtle-suppon)powder administered to the rat[J].Food&Chemical Toxicology,2005,43(4):575-580
[3]Li S Z,Yuan X J,Yang Y Y,et al.Effect of cold stress by immersing in water on some enzyme activities and digestive tract mucosal hyperemia in chicken[J].Applied and Environmental Biology,2001,7(3):267-270[李士泽,袁学军,杨玉英,等.浸水冷应激对雏鸡某些酶活性及消化道黏膜充血的影响.应用与环境生物学报,2001,7(3):267-270]
[4]Kaushik S,Kaur J.Effect of chronic cold stress on intestinal epithelial cell proliferation and inflammation in rats[J].Stress-the International Journal on the Biology of Stress,2005,8(3):191-197
[5]Walser E M,Nealon W H,Marroquin S,et al.Sterile fluid collections in acute pancreatitis:catheter drainage versus simple aspiration[J].Cardiovascular&Interventional Radiology,2006,29(1):102-107
[6]Niu H J,Wang B M.Intestinal mucosal barrier and its function[J].Anatomy and Clinics,2007,12(2):138-140[牛海静,汪邦茂.肠黏膜屏障与功能.解剖与临床,2007,12(2):138-140]
[7]Cheng L Z.Histology(second edition)[M].Beijing:Peoples Medical Press.1994,1102-1125[成令忠.组织学(第2版).北京:人民卫生出版社.1994,1102-1125]
[8]Reynolds J V.Gut barrier function in the surgical patients[J].British Journal of Surgery,1996,83(12):1668-1671
[9]Fukudome I,Kobayashi M,Dabanaka K,et al.Diamine oxidase as a maker of intestinal mucosal injury and the effect of soluble dietary fiber on gastrointestinal tract toxicity after intravenous 5-fluourouracil treatment in rats[J].Medical Molecular Morphology,2014,47(2):100-107
[10]Karabulut K U,Narci H,Gul M,et al.Diamine oxidase in diagnosis of acute mesenteric ischemia[J].American Journal of Emergency Medicine,2013,31(2):309-312
[11]Wu W M,Zhang F X,Zhang P,et al.Influence of plateau hypoxia on the tissue injury and expression of HIF-1αand iNOS in intestinal mucosa of rats[J].Medical Journal of Chinese People’s Liberation Army,2010,35(5):592-594[吴文明,张方信,张盼,等.高原缺氧条件对大鼠肠黏膜组织及缺氧诱导因子一1α、诱导型-一氧化氮合酶表达的影响.解放军医学杂志,2010,35(5):592-594]
[12]Bonous G,Echave V,Vobecky S J,et al.Acute necrosis of the intestinal muesoa and high serum levels of diamine oxidase[J].Digestive Diseases and Sciences,1984,29(9):872-874
[13]Luk G D,Bayless T M,Baylin S B.Diamine oxidase(DAO)a circulating marker for rat mucosal maturation and integrity[J].Journal of Clinical Investigation,1980,66(1):66-70
[14]Nakao M,Ogura Y,Satake S,et al.Usefulness of soluble dietary fiber for the treatment of diarrhea during enteral nutrition in elderly patients[J].Nutrition,2002,18(1):35-39
[15]Fu X B,Xing F,Yang Y H,et al.Activation of phosphorylating-p38 mitogen-activated protein kinase and its relationship with localization of intestinal stem cells in rats after ischemiareperfusion injury[J].World Journal of Gastroenterology,2003,9(9):2036-2039
[16]Li J Y,Sheng Z Y,LüY,et al.Severe trauma induced intestinal barrier function injury and protection[J].World Chinese Journal of Digestology,2000,8(10):1093-1096[黎君友,盛志勇,吕艺,等.严重创伤后肠屏障功能损伤及谷氨酰胺的保护.世界华人消化杂志,2000,8(10):1093-1096]
[17]Mantis N J,Cheung M C,Chintalcharuvu K R,et al.Selective adherence of IgA to murine Peyer's patch M cells:evidence for a novel IgA receptor[J].Journal of Immunology,2002,169(4):1844-1851
[18]Wang Z L,Yu T Q,Zhu X Y,et al.Effects of complex prescriptions of Chinese herbs on the concentration of IL-2,IL-10 and IgA in porcine intestines after heat stress[J].Chinese Journal of Veterinary Medicine,2007,43(9):83-85[王自力,于同泉,朱晓宇,等.中药复方对热应激下猪肠道组织IL-2 IL-10和黏液IgA含量影响.中国兽药杂志,2007,43(9):83-85]
[19]Bai X D.Intestinal mucous layer research progress[J].Foreign Medicine:Volumes of Trauma and Surgical Problems,1997,(4):200-202[白晓东.肠道黏液层研究进展.国外医学:创伤与外科基本问题分册,1997,(4):200-202]
[20]Olsen R E,Sundell K,Mayhew T M,et al.Acute stress alters intestinal function of rainbow trout,Oncorhynchus mykiss[J].Aquaculture,2005,250(1-2):480-495
[21]Fu J,Liu C P,Zhang Z W,et al.Influence of inflammatory pathway markers on oxidative stress induced by cold stress in intestine of quails[J].Research in Veterinary Science,2013,95(2):495-501
[22]Glaser R,Kiecoltglaser J K.Stress-induced immune dys-function:implications for health[J].Nature Reviews Immunology,2005,5(3):243-251
[23]Zhu G F,Chancellor-Freeland C,Berman A S,et al.Endogenous substance P mediates cold water stress-induced increase in interleukin-6 secretion from peritoneal macrophages[J].Journal of Neuroscience the Official Journal of Society for Neuroscience,1996,16(11):3745-3752
[24]Zhang W.Effects of cold stress on chicken’s barrier function of intestinal mucosa[D].The Northeast Agricultural University.2010[张雯.冷应激对雏鸡肠黏膜屏障功能的影响.东北农业大学.2010]
[25]Han Z K.Livestock Nutrition Physiology[M].Beijing:Agriculture Press.1991,16-17[韩正康.家畜营养生理学.北京:农业出版社.1991,16-17]