乳腺癌中凋亡细胞比率与雌激素受体、孕激素受体、人表皮生长因子-2表达的相关性研究
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摘要
目的探讨乳腺癌凋亡细胞比率与ER、PR、HER-2表达的相关性。方法通过对91例癌灶组织流式细胞术检测癌灶组织中的凋亡细胞比率,免疫组化法监测癌灶组织中雌激素受体、孕激素受体、人类表皮生长因子受体2表达,对HER-2(++)进行FISH检测最终确定阴阳性表达。通过统计学分析确定乳腺癌细胞凋亡细胞比率与乳腺癌免疫组化中ER、PR、HER-2表达的相关性。结果不同临床参数患者凋亡细胞比率比较,差异无统计学意义(P> 0. 05),雌激素受体、孕激素受体皆阴性患者凋亡细胞比率均显著高于阳性患者,差异有统计学意义(P <0. 05),凋亡细胞比率与雌激素受体、孕激素受体呈正相关(r=0. 982,P <0. 05),凋亡细胞比率较高者与人类表皮生长因子受体2受体表达阳性,呈正相关(r=0. 985,P <0. 05)。结论凋亡细胞比率与雌激素受体、孕激素受体阴性表达、人类表皮生长因子受体2受体表达阳性显著相关。
Objective To investigate the correlation of apoptotic cell ratio with ER,PR and Her-2 levels in breast cancer.Methods Flow cytometry was used to monitor the apoptotic cell rate in 91 cases of cancer tissue,and the expressions of estrogen receptor,progesterone receptor and human epidermal growth factor receptor 2 were detected by immunohistochemistry.HER-2( + +) was conducted for FISH monitoring so as to determine positive and negative expressions. Statistical analysis was used to determine the correlation of apoptotic cell rate with ER,PR and HER-2 levels in breast cancer. Results There was no statistical significance on the difference of apoptosis cell ratio in patients with different clinical parameters( P > 0. 05);and the apoptosis cell ratio in patients with negative estrogen receptor and progesterone receptor was significantly higher than that of positive patients,with the differences statistically significant( P < 0. 05). Apoptosis cell ratio was positively correlated with estrogen receptor and progesterone receptor( r = 0. 982,P < 0. 05),and negatively correlated with HER-2 receptors( r =0. 985,P < 0. 05). Conclusion Apoptotic cell ratio was significantly associated with progesterone receptor and the negative expression of estrogen receptor,and positively associated with human epidermal growth factor 2.
Objective To investigate the correlation of apoptotic cell ratio with ER,PR and Her-2 levels in breast cancer.Methods Flow cytometry was used to monitor the apoptotic cell rate in 91 cases of cancer tissue,and the expressions of estrogen receptor,progesterone receptor and human epidermal growth factor receptor 2 were detected by immunohistochemistry.HER-2( + +) was conducted for FISH monitoring so as to determine positive and negative expressions. Statistical analysis was used to determine the correlation of apoptotic cell rate with ER,PR and HER-2 levels in breast cancer. Results There was no statistical significance on the difference of apoptosis cell ratio in patients with different clinical parameters( P > 0. 05);and the apoptosis cell ratio in patients with negative estrogen receptor and progesterone receptor was significantly higher than that of positive patients,with the differences statistically significant( P < 0. 05). Apoptosis cell ratio was positively correlated with estrogen receptor and progesterone receptor( r = 0. 982,P < 0. 05),and negatively correlated with HER-2 receptors( r =0. 985,P < 0. 05). Conclusion Apoptotic cell ratio was significantly associated with progesterone receptor and the negative expression of estrogen receptor,and positively associated with human epidermal growth factor 2.
引文
[1] Kochupurakkal BS,Wang ZC,Hua T,et al. Rel A-Induced Interferon Response Negatively Regulates Proliferation[J]. Plos One,2015,10(10):e0140243.
[2]杨壹羚,范宇,郎荣刚,等.美国临床肿瘤学协会(ASCO)/美国病理医师学院(CAP)推荐新指南HER2检测技术方法的建立及应用[J].中国肺癌杂志,2009,31(6):672-672.
[3]中国抗癌协会乳腺癌专业委员会.中国抗癌协会乳腺癌诊治指南与规范(2013版)[J].中国癌症杂志,2013,19(8):410-428.
[4]陈伟财,王先明,佘晓佳,等.乳腺癌ER,PR和HER-2的表达状况与新辅助化疗疗效的临床观察[J].山西医科大学学报,2010,41(7):599-602.
[5]郭欢续,白欧.乳腺癌ER、PR、Her-2表达与临床病理特征[J].中国实验诊断学,2008,12(11):1390-1393.
[6]杨丽丽,梁莉萍,赵峰,等. ER、PR、HER-2、Ki67、Nm23、P53在多中心性乳腺癌中的表达及临床意义[J].重庆医科大学学报,2012,37(11):964-967.
[7]吕有勇,许小青.北京大学医学部研究生分子肿瘤学课堂讨论及学术论坛纪要[J].世界华人消化杂志,2003,11(7):1031-1034.
[8]李文婷,王莹,张银华,等.年轻女性乳腺癌中ER、PR、HER-2和Ki67的表达与临床病理意义[J].重庆医科大学学报,2012,37(9):783-786.
[9]汪海新,应明真,王梅,等. ER、PR、HER-2、P53在乳腺浸润性导管癌组织中的表达及临床意义[J].医学研究杂志,2013,42(2):27-31.
[10]王娇,温健,涂巍,等. ER和PR及HER-2表达与乳腺癌分子分型及临床特征和预后的关系[J].中国现代普通外科进展,2014,17(2):99-103.
[11] Kochupurakkal BS,Wang ZC,Hua T,et al. Rel A-Induced Interferon Response Negatively Regulates Proliferation[J]. Plos One,2015,10(10):e0140243.
[12] Lee JA,Bae JW,Sang UW,et al. Correlation between COX-2Expression and Hormone Receptors in Invasive Ductal Breast Cancer[J]. Ann Surg Tre Res,2010,78(3):140-148.
[13]甄乐锋,叶长生,刘民锋,等.青年乳腺癌中ER、PR、HER-2及VEGF表达及其临床病理学意义[J].广东医学,2010,31(4):490-492.
[14]谭先华,夏述琳,欧阳焱黎,等.乳腺癌钼靶X线特征与ER、PR和HER-2的相关性研究[J].临床放射学杂志,2009,28(12):1618-1622.
[15] Liu J,Wang L,Gao W,et al. Inhibitory receptor immunoglobulinlike transcript 4 was highly expressed in primary ductal and lobular breast cancer and significantly correlated with IL-10[J]. Diagn Pathol,2014,9(1):85.
[16]张景臣,刘薇,毛大华,等. S期细胞比率与乳腺癌免疫组化中ER、PR、HER-2表达相关性研究[J].临床心身疾病杂志,2017,23(3):10-11.
[2]杨壹羚,范宇,郎荣刚,等.美国临床肿瘤学协会(ASCO)/美国病理医师学院(CAP)推荐新指南HER2检测技术方法的建立及应用[J].中国肺癌杂志,2009,31(6):672-672.
[3]中国抗癌协会乳腺癌专业委员会.中国抗癌协会乳腺癌诊治指南与规范(2013版)[J].中国癌症杂志,2013,19(8):410-428.
[4]陈伟财,王先明,佘晓佳,等.乳腺癌ER,PR和HER-2的表达状况与新辅助化疗疗效的临床观察[J].山西医科大学学报,2010,41(7):599-602.
[5]郭欢续,白欧.乳腺癌ER、PR、Her-2表达与临床病理特征[J].中国实验诊断学,2008,12(11):1390-1393.
[6]杨丽丽,梁莉萍,赵峰,等. ER、PR、HER-2、Ki67、Nm23、P53在多中心性乳腺癌中的表达及临床意义[J].重庆医科大学学报,2012,37(11):964-967.
[7]吕有勇,许小青.北京大学医学部研究生分子肿瘤学课堂讨论及学术论坛纪要[J].世界华人消化杂志,2003,11(7):1031-1034.
[8]李文婷,王莹,张银华,等.年轻女性乳腺癌中ER、PR、HER-2和Ki67的表达与临床病理意义[J].重庆医科大学学报,2012,37(9):783-786.
[9]汪海新,应明真,王梅,等. ER、PR、HER-2、P53在乳腺浸润性导管癌组织中的表达及临床意义[J].医学研究杂志,2013,42(2):27-31.
[10]王娇,温健,涂巍,等. ER和PR及HER-2表达与乳腺癌分子分型及临床特征和预后的关系[J].中国现代普通外科进展,2014,17(2):99-103.
[11] Kochupurakkal BS,Wang ZC,Hua T,et al. Rel A-Induced Interferon Response Negatively Regulates Proliferation[J]. Plos One,2015,10(10):e0140243.
[12] Lee JA,Bae JW,Sang UW,et al. Correlation between COX-2Expression and Hormone Receptors in Invasive Ductal Breast Cancer[J]. Ann Surg Tre Res,2010,78(3):140-148.
[13]甄乐锋,叶长生,刘民锋,等.青年乳腺癌中ER、PR、HER-2及VEGF表达及其临床病理学意义[J].广东医学,2010,31(4):490-492.
[14]谭先华,夏述琳,欧阳焱黎,等.乳腺癌钼靶X线特征与ER、PR和HER-2的相关性研究[J].临床放射学杂志,2009,28(12):1618-1622.
[15] Liu J,Wang L,Gao W,et al. Inhibitory receptor immunoglobulinlike transcript 4 was highly expressed in primary ductal and lobular breast cancer and significantly correlated with IL-10[J]. Diagn Pathol,2014,9(1):85.
[16]张景臣,刘薇,毛大华,等. S期细胞比率与乳腺癌免疫组化中ER、PR、HER-2表达相关性研究[J].临床心身疾病杂志,2017,23(3):10-11.