生脉颗粒质量标准研究
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摘要
目的:建立生脉颗粒质量标准控制方法。方法:采用TLC五味子薄层鉴别方法以及HPLC法测定五味子中五味子醇甲的含量。色谱柱:phenomenex Luna C18(150×4.60 mm,5μm);流动相:甲醇-0.1%磷酸溶液(60∶40);流速:1.0 m L/min;检测波长:250 nm。结果:薄层鉴别方法简单,阴性无干扰;五味子醇甲在0.0712~2.136μg呈良好的线性关系,r=0.999 9,平均回收率为97.23%;结论:该方法简单、快速、专属性强、可操作性强。
Objective: To establish a method for quality standard of Sheng Mai granules. Methods: The contents of schisandrin in Wu Wei Zi(Schisandra chinensis) were determined by HPLC and distinguished by TLC.Chromatographic column: phenomenex Luna C18(150×4.60 mm, 5 μm); mobile phase: methyl alcohol-0.1% phosphoric acid solution(60∶40); flow rate: 1.0 m L/min; detection wavelength: 250 nm. Results: TLC method was simple and the blank test demonstrated no interference. Schisandrin demonstrated better linear relationship in the range between 0.0 712 and 2.136 μg, r=0.9 99 9, average recovery rate was 97.23%. Conclusion: The method is simple, rapid, specific and feasible.
Objective: To establish a method for quality standard of Sheng Mai granules. Methods: The contents of schisandrin in Wu Wei Zi(Schisandra chinensis) were determined by HPLC and distinguished by TLC.Chromatographic column: phenomenex Luna C18(150×4.60 mm, 5 μm); mobile phase: methyl alcohol-0.1% phosphoric acid solution(60∶40); flow rate: 1.0 m L/min; detection wavelength: 250 nm. Results: TLC method was simple and the blank test demonstrated no interference. Schisandrin demonstrated better linear relationship in the range between 0.0 712 and 2.136 μg, r=0.9 99 9, average recovery rate was 97.23%. Conclusion: The method is simple, rapid, specific and feasible.
引文
[1]中华人民共和国卫生部药典委员会.中华人民共和国卫生部药品标准:中药成方制剂:第11册[M].北京:化学工业出版社,1996:46.
[2]中华人民共和国卫生部药典委员会.中华人民共和国药典中药薄层色谱彩色图籍[M].广州:广东科技出版社,1993:30.
[3]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2010:61.
[4]赵晓春,凌俊英,赵志军.护肝片中五味子薄层鉴别方法的改进[J].中成药.1994:16(1):19.
[5]吴万征,林焕泽,吴秀荣.橘红梨膏中化橘红、五味子的薄层鉴别[J].中国药师,2005,8(5):432-433.
[6]苗明三,李振国.现代实用中药质量控制技术[M].北京:人民卫生出版社,2000:199-207.
[7]欧金梅,罗捷华.HPLC法测定生脉饮中五味子醇甲含量[J].安徽中医学院学报,2006,25(1):33-35.
[8]韦向红,唐敏玲.HPLC法测定生脉饮(党参方)中五味子醇甲含量[J].广西中医药,2005,28(4):57-58.
[9]于超,王宇,王善全,等.高效液相色谱测定五味子及其制剂中五味子醇甲的含量[J].基层中药杂志,2001,15(2):13-15.
[10]李英,陈忠樑.HPLC测定安神补心丸中五味子醇甲和丹参酮ⅡA含量[J].中国保健,2004,26(7):538-540.
[11]何海冰,唐星,于叶淼.生脉粉针剂中五味子醇甲和总木脂素的含量测定[J].沈阳药科大学学报,2005,22(4):282-285.
[2]中华人民共和国卫生部药典委员会.中华人民共和国药典中药薄层色谱彩色图籍[M].广州:广东科技出版社,1993:30.
[3]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2010:61.
[4]赵晓春,凌俊英,赵志军.护肝片中五味子薄层鉴别方法的改进[J].中成药.1994:16(1):19.
[5]吴万征,林焕泽,吴秀荣.橘红梨膏中化橘红、五味子的薄层鉴别[J].中国药师,2005,8(5):432-433.
[6]苗明三,李振国.现代实用中药质量控制技术[M].北京:人民卫生出版社,2000:199-207.
[7]欧金梅,罗捷华.HPLC法测定生脉饮中五味子醇甲含量[J].安徽中医学院学报,2006,25(1):33-35.
[8]韦向红,唐敏玲.HPLC法测定生脉饮(党参方)中五味子醇甲含量[J].广西中医药,2005,28(4):57-58.
[9]于超,王宇,王善全,等.高效液相色谱测定五味子及其制剂中五味子醇甲的含量[J].基层中药杂志,2001,15(2):13-15.
[10]李英,陈忠樑.HPLC测定安神补心丸中五味子醇甲和丹参酮ⅡA含量[J].中国保健,2004,26(7):538-540.
[11]何海冰,唐星,于叶淼.生脉粉针剂中五味子醇甲和总木脂素的含量测定[J].沈阳药科大学学报,2005,22(4):282-285.