枝孢霉菌生长规律及对喷气燃料性质的影响
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摘要
为探讨特征真菌对喷气燃料性质的影响,构建了培养液和喷气燃料培养体系,每4 h检测培养体系水相中枝孢霉菌的发光强度;每24 h检测燃料相中喷气燃料的总酸值、表面张力、水分离等级、银片腐蚀等级以及水相pH等理化指标,分别以时间和发光强度为横、纵坐标绘制生长曲线,测定喷气燃料总酸值、表面张力等指标的变化规律。结果表明:特征真菌的生长繁殖会增大喷气燃料的总酸值,降低喷气燃料表面张力和溶解水的pH,影响喷气燃料的腐蚀性和洁净性;水相发光强度达到400 RLU/mL时,枝孢霉菌开始对数繁殖,喷气燃料下部样品被重度污染。
To investigate the effect of characteristic fungi on jet fuel properties of jet fuel,a culture medium and a jet fuel culture system were constructed.The luminescence intensity in the aqueous phase was detected every 4 hours,and the physical and chemical indicators such as total acid number,surface tension,water separation grade,silver corrosion grade and pH value of jet fuel,were detected every 24 hours.The growth curves were plotted with time;luminescence intensity and the change of total acid number and surface tension of jet fuel were obtained.The results showed that the growth and reproduction of characteristic fungi increased the total acid value of jet fuel,while decreased the surface tension of jet fuel and lowered the pH value of dissolved water,and affected the corrosiveness and cleanliness of jet fuel.Cladosporium began to proliferate logarithmically and the bottom of the jet fuel was heavily contaminated when the luminescence intensity in the water phase reached 400 RLU/mL.
To investigate the effect of characteristic fungi on jet fuel properties of jet fuel,a culture medium and a jet fuel culture system were constructed.The luminescence intensity in the aqueous phase was detected every 4 hours,and the physical and chemical indicators such as total acid number,surface tension,water separation grade,silver corrosion grade and pH value of jet fuel,were detected every 24 hours.The growth curves were plotted with time;luminescence intensity and the change of total acid number and surface tension of jet fuel were obtained.The results showed that the growth and reproduction of characteristic fungi increased the total acid value of jet fuel,while decreased the surface tension of jet fuel and lowered the pH value of dissolved water,and affected the corrosiveness and cleanliness of jet fuel.Cladosporium began to proliferate logarithmically and the bottom of the jet fuel was heavily contaminated when the luminescence intensity in the water phase reached 400 RLU/mL.
引文
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[2] Passman F J.Microbial contamination and its control in fuels and fuel systems since 1980:A review[J].International Biodeterioration & Biodegradation,2013,81:88-104
[3] 熊云,许世海,刘晓,等.油品应用及管理[M].3版.北京:中国石化出版社,2015:3
[4] 许世海,熊云,刘晓.液体燃料的性质及应用[M].北京:中国石化出版社,2010:141-142
[5] 袁祥波,陈学军,熊云.喷气燃料絮状悬浮物的形成机理探讨[J].石油炼制与化工,2015,46(11):74-77
[6] Itah A Y,Brooks A A,Ogar B O,et al.Biodegradation of international jet A-1 aviation fuel by microorganisms isolated from aircraft tank and joint hydrant storage systems[J].Bulletin of Environmental Contamination and Toxicology,2009,83(3):318-327
[7] Ferrari M D,Neirotti E,Albornoz C.Occurrence of heterotrophic bacteria and fungi in an aviation fuel handling system and its relationship with fuel fouling[J].Revista Argentina de Microbiologia,1998,30(3):105-114
[8] 袁祥波,胡启文,熊云,等.储存喷气燃料中特征真菌的鉴定与生长特性[J].后勤工程学院学报,2014,30(1):59-63
[9] 杨浩,熊云,和倩倩,等.喷气燃料中主要污染真菌的确定[J].工业微生物,2015,45(6):37-42
[10] 李鹏.喷气燃料杀菌剂的应用基础研究[D].重庆:陆军勤务学院,2017
[11] 路福平.微生物学[M].北京:中国轻工业出版社,2015:4
[12] 丛苑,卢彦廷,杜奕君,等.ATP发光法快速检测玉米中的霉菌[J].中国食品学报,2014(8):233-239
[13] Thore A,Ansehn A,Lundin A,et al.Detection of bacteriuria by luciferase assay of adenosine-triphosphate[J].Journal of Clinical Microbiology,1975,1(1):1-8
[14] IATA.Guidance Material on Microbiological Contamination in Aircraft Fuel Tanks(5th)[S].2015
[15] 袁祥波,梅洋,熊云,等.不同孔径滤膜对喷气燃料质量的影响[J].后勤工程学院学报,2014,30(4):33-36,48