鼻气管鸟杆菌单克隆抗体的防治效果
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摘要
利用单克隆制备技术制备了两株稳定分泌针对鼻气管鸟杆菌外膜蛋白OMA87的单克隆抗体的杂交瘤细胞系,并对其进行株特异性鉴定、效价鉴定、Western-blot、中和试验及防治效果鉴定,选择其中2株高沉淀效价及中和效价的单克隆抗体(2C4和5G9)进行了鼻气管鸟杆菌引起的呼吸道疾病防治试验,抗体治愈率分别达到95%和80%,具有良好的防治效果,在临床应用上具有较高的实用价值。
引文
[1] EMPEL P C M V, HAFEZ H M. Ornithobacterium rhinotracheale:A review[J]. Avian Pathology, 1999, 28(3):217-227.
[2]赵鹏,李佳,谢琛,等.家禽鼻气管鸟疫杆菌研究进展[J].中国家禽,2015,37(6):37-41.
[3]马保华,毕英佐,吕平,等.鼻气管鸟杆菌病的研究进展[J].中国预防兽医学报,2002,24(3):232-234.
[4] LI Y Y, QIN Y B, WANG W, et al. Progress of Omithobacterium rhinotracheale in domestic chicken flock[J].Poultry Scienc, 2010,(11):40-43.
[5]陈小玲,宋程,罗廷荣,等.鼻气管鸟疫杆菌中国株的初步鉴定[C]//中国畜牧兽医学会禽病学会分会第十次学术研讨会论文集,2000.
[6]陈小玲,宋程,罗廷荣,等.鼻气管鸟疫杆菌中国株的鉴定[J].中国家禽,2004,26(18):8-9.
[7]薛营.鼻气管鸟杆菌分离鉴定与OMA87蛋白单克隆抗体制备[D].扬州:扬州大学,2017.
[8] VANDAMME P, SEGERS P, VANCANNVEYT M, et al.Ornithobacterium rhinotracheale gen. nov. sp. nov. isolated from the avian respiratory tract[J]. International Journal of Systematic Bacteriology, 1994, 44(1):24.
[9] VAN E P, VAN D B H, LOEFFEN P, et al. Identification and serotyping of Ornithobacterium rhinotracheale[J].Journal of Clinical Microbiology, 1997, 35(2):418.
[10] VAN V L, VAN EMPEL C P, FABRIA T. Ornithobacterium rhinotracheale, a primary pathogen in broilers[J]. Avian Diseases, 2000, 44(4):896.
[12] SCHUIJFFEL D F, VAN EMPEL P C, PENNINGS A M,et al. Passive immunization of immune-suppressed animals:Chicken antibodies protect against Ornithobacterium rhinotracheale infection[J]. Vaccine, 2005, 23(26):3404-3411.
[12]薛营,杨开典,林伟东,等.肉鸡鼻气管炎鸟杆菌的分离与鉴定[J].中国兽医科学,2016(9):1135-1140.
[2]赵鹏,李佳,谢琛,等.家禽鼻气管鸟疫杆菌研究进展[J].中国家禽,2015,37(6):37-41.
[3]马保华,毕英佐,吕平,等.鼻气管鸟杆菌病的研究进展[J].中国预防兽医学报,2002,24(3):232-234.
[4] LI Y Y, QIN Y B, WANG W, et al. Progress of Omithobacterium rhinotracheale in domestic chicken flock[J].Poultry Scienc, 2010,(11):40-43.
[5]陈小玲,宋程,罗廷荣,等.鼻气管鸟疫杆菌中国株的初步鉴定[C]//中国畜牧兽医学会禽病学会分会第十次学术研讨会论文集,2000.
[6]陈小玲,宋程,罗廷荣,等.鼻气管鸟疫杆菌中国株的鉴定[J].中国家禽,2004,26(18):8-9.
[7]薛营.鼻气管鸟杆菌分离鉴定与OMA87蛋白单克隆抗体制备[D].扬州:扬州大学,2017.
[8] VANDAMME P, SEGERS P, VANCANNVEYT M, et al.Ornithobacterium rhinotracheale gen. nov. sp. nov. isolated from the avian respiratory tract[J]. International Journal of Systematic Bacteriology, 1994, 44(1):24.
[9] VAN E P, VAN D B H, LOEFFEN P, et al. Identification and serotyping of Ornithobacterium rhinotracheale[J].Journal of Clinical Microbiology, 1997, 35(2):418.
[10] VAN V L, VAN EMPEL C P, FABRIA T. Ornithobacterium rhinotracheale, a primary pathogen in broilers[J]. Avian Diseases, 2000, 44(4):896.
[12] SCHUIJFFEL D F, VAN EMPEL P C, PENNINGS A M,et al. Passive immunization of immune-suppressed animals:Chicken antibodies protect against Ornithobacterium rhinotracheale infection[J]. Vaccine, 2005, 23(26):3404-3411.
[12]薛营,杨开典,林伟东,等.肉鸡鼻气管炎鸟杆菌的分离与鉴定[J].中国兽医科学,2016(9):1135-1140.