生物统计法分析T-2毒素对凡纳滨对虾形态指标的影响
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摘要
以不同剂量T-2毒素染毒的凡纳滨对虾(Litopenaeus vannamei)为研究对象,以毒素染毒剂量为自变量、凡纳滨对虾形态学指标为因变量,采用灰色关联度分析、通径分析和回归分析探究毒素剂量与染毒凡纳滨对虾形态学指标的相关性,筛选出最便捷、最简单的生物学统计方法。灰色关联度和通径分析显示,凡纳滨对虾体质量、体长、头胸甲长和第一腹宽是受T-2毒素影响最大的指标;利用回归分析进行显著性检验发现,T-2毒素对凡纳滨对虾体质量、体长、头胸甲长和第一腹宽指标的影响均为显著水平(P<0.05)。3种统计学方法得出的结果相吻合,体质量、体长、头胸甲长、第一腹宽等染毒凡纳滨对虾形态指标的重要生物学标记,均与T-2毒素的暴露剂量呈负相关。其中,灰色关联度分析法是最便捷、最简单的生物统计法。
Litopenaeus vannamei was adopted as the research object in this study, which was treated with different doses of T-2 toxin. T-2 toxin dose was adopted as the independent variable, along with morphological indicators of shrimp as the dependent variable. To investigate the correlation within the dose of T-2 toxin and various morphological indexes of treated shrimp, common statistical analysis methods were used including gray correlation analysis method, path analysis and regression analysis. The most convenient and simple biological statistics were filtered out. The experimental results indicated that the body weight, body length, carapace length and the first abdominal segment width of toxic shrimp morphological index were the most influential indexes of T-2 toxin as analyzed by gray correlation and path analysis. The significance test performed by regression analysis showed that the effects of T-2 toxin on body weight, body length, carapace length and the first abdominal segment width of treated shrimp were significant(P<0.05). The results of the three statistical methods were consistent. Body weight, body length, carapace length and the first abdominal segment width were the important morphological indicators of toxic shrimp, and they were negatively correlated with the exposure dose of T-2 toxin. And gray correlation analysis was the most convenient and easiest biological statistics.
Litopenaeus vannamei was adopted as the research object in this study, which was treated with different doses of T-2 toxin. T-2 toxin dose was adopted as the independent variable, along with morphological indicators of shrimp as the dependent variable. To investigate the correlation within the dose of T-2 toxin and various morphological indexes of treated shrimp, common statistical analysis methods were used including gray correlation analysis method, path analysis and regression analysis. The most convenient and simple biological statistics were filtered out. The experimental results indicated that the body weight, body length, carapace length and the first abdominal segment width of toxic shrimp morphological index were the most influential indexes of T-2 toxin as analyzed by gray correlation and path analysis. The significance test performed by regression analysis showed that the effects of T-2 toxin on body weight, body length, carapace length and the first abdominal segment width of treated shrimp were significant(P<0.05). The results of the three statistical methods were consistent. Body weight, body length, carapace length and the first abdominal segment width were the important morphological indicators of toxic shrimp, and they were negatively correlated with the exposure dose of T-2 toxin. And gray correlation analysis was the most convenient and easiest biological statistics.
引文
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[18] 杜家菊, 陈志伟. 使用SPSS线性回归实现通径分析的方法[J]. 生物学通报, 2010, 45(2): 4-5.DU J J, CHEN Z W. Method of path analysis with SPSS linear regression[J]. Bulletin of Biology, 2010, 45(2): 4-5.(in Chinese with English abstract)
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[20] 胡贤德, 孙成波, 李镇泉, 等. 北部湾墨吉明对虾和斑节对虾形态性状对体质量的影响分析[J]. 海南大学学报(自然科学版): 2009, 27(1): 48-53.HU X D, SUN C B, LI Z Q, et al. Relation of morphological attributes on body weight for wild Fenneropenaeus merguiensis and Penaeus monodon from Beibu Bay[J]. Natural Science Journal of Hainan University, 2009, 27(1): 48-53.(in Chinese with English abstract)
[2] 黄展锐, 王雅玲, 王小博, 等. 对虾中隐蔽态T-2毒素残留对小鼠血常规和血清生化指标的影响[J]. 浙江农业学报, 2017, 29(7): 1110-1118.HUANG Z R, WANG Y L, WANG X B, et al. Effect of masked T-2 toxin residues in Litopenaeus vannamei on routine blood and serum biochemical indexes of mice[J]. Acta Agricultural Zhejiangensis, 2017, 29(7): 1110-1118. (in Chinese with English abstract)
[3] 吕旌乔, 王治伦, 吕社民, 等. T-2毒素研究进展[J]. 中国地方病防治杂志, 1996, 11(5): 282-285.LYU J Q, WANG Z L, LYU S M, et al. Research advances on T-2 toxin[J]. Chinese Journal of Control of Endemic Diseases, 1996, 11(5): 282-285.(in Chinese)
[4] 施琦. T-2毒素的自然发生与降解及其在对虾中的蓄积规律[D]. 湛江:广东海洋大学, 2013.SHI Q. T-2 toxin accuring and degradation in natural enviromemt and its accumulation in Litopenaeus vannamei[D]. Zhanjiang: Guangdong Ocean University, 2013.(in Chinese with English abstract)
[5] 代喆. T-2毒素诱导凡纳滨对虾肌肉品质典型性状的变化规律[D]. 湛江:广东海洋大学, 2013.DAI Z. Regulation of Litopenaeus vannamei muscle quota effected by T-2 toxin[D]. Zhanjiang: Guangdong Ocean University, 2013.(in Chinese with English abstract)
[6] 董世瑞, 孔杰, 万初坤, 等. 中国对虾形态性状对体重影响的通径分析[J]. 渔业科学进展, 2007, 28(3): 15-22.DONG S R, KONG J, WANG C K, et al. Path analysis of effects of morphometric attributes on body weight of Fenneropenaeus chinensis[J]. Progress in Fishery Sciences, 2007, 28(3): 15-22.(in Chinese with English abstract)
[7] 梁光明. T-2毒素诱导凡纳滨对虾肌肉蛋白质变化的规律[D]. 湛江:广东海洋大学, 2014.LIANG G M. The change rule for muscle protein of Litopenaeus vannamei by T-2 toxin induced[D]. Zhanjiang: Guangdong Ocean University, 2014.(in Chinese with English abstract)
[8] 盖钧镒. 试验统计方法[M]. 北京:中国农业出版社, 2000.
[9] 李玉全. 线性回归法实现半滑舌鳎幼鱼表型性状与体重的通径分析[J]. 生物数学学报, 2014,29(1): 185-191.LI Y Q. Path Analysis between morphometric attributes and body weight on juvenile Cynoglossus Semilaevis by multiple regression[J]. Journal of Biomathematics, 2014,29(1): 185-191.(in Chinese with English abstract)
[10] LU P, WANG Y, DAI Z, et al. Selection and evaluation of the typical indexes of Litopenaeus vannamei contaminated T-2 toxin by gray relational method[J]. Journal of Aquatic Animal Health, 2017(29): 129-135.
[11] 王志铮, 吴一挺, 杨磊, 等. 日本沼虾形态性状对体重的影响效应[J]. 海洋与湖沼, 2011, 42(4): 612-618.WANG Z Z, WU Y T, YANG L, et al. Effects of phenotypic and morphometric traits on body weight of Macrobrachium Nipponensis[J]. Oceanologia et Limnologia Sinica, 2011, 42(4): 612-618.(in Chinese with English abstract)
[12] 孙芳芳. 浅议灰色关联度分析方法及其应用[J]. 科技信息, 2010(17): 880-882.SUN F F. Discussion on the method of grey relational analysis and its application[J]. Science & Technology Information, 2010(17): 880-882.(in Chinese)
[13] 刘小林,吴氏功,张志怀, 等. 凡纳对虾形态性状对体重的影响效果分析[J]. 生态学报, 2004, 24(4): 857-862. LIU X L, WU S G, ZHANG Z H, et al. Mathematical analysis of effects of morphometric attributes on body weight for Penaeus vannamei[J]. Acta Ecologica Sinica, 2004, 24(4): 857-862.(in Chinese with English abstract)
[14] 任红松, 朱家辉, 杨斌, 等. Excel在通径分析中的应用[J]. 农业网络信息, 2006(3): 90-92.REN H S, ZHU J H, YANG B, et al. Excel application in path analysis[J]. Agriculture Network Information, 2006(3): 90-92.(in Chinese)
[15] 孙苗苗, 陈百尧, 杨其彬, 等. 斑节对虾形态性状对体质量影响的分析[J]. 海洋科学, 2013(5): 50-53.SUN M M, CHEN B Y, YANG Q B, et al. Effects of morphological characters on body weight of Penaeus monodon[J]. Marine Sciences, 2013(5): 50-53.(in Chinese with English abstract)
[16] 安丽, 刘萍, 李健, 等. “黄海1号”中国明对虾形态性状对体质量的影响效果分析[J]. 中国水产科学, 2008, 15(5): 780-786.AN L, LIU P, LI J, et al. Mathematical analysis of effects of morphometric traits on body weight for Fenneropenaeus chinensis named “Yellow Sea No. 1”[J]. Journal of Fishery Sciences of China, 2008, 15(5): 780-786.(in Chinese with English abstract)
[17] 杨长明, 何桐, 刘小林, 等. 凡纳滨对虾形态性状对体质量的逐步回归分析[J]. 西北农业学报, 2011, 20(2): 15-20. YANG C M, HE T, LIU X L, et al. Stepwise regression analysis of morphometric attributes on body weight of Penaeus vannamei[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2011, 20(2): 15-20.(in Chinese with English abstract)
[18] 杜家菊, 陈志伟. 使用SPSS线性回归实现通径分析的方法[J]. 生物学通报, 2010, 45(2): 4-5.DU J J, CHEN Z W. Method of path analysis with SPSS linear regression[J]. Bulletin of Biology, 2010, 45(2): 4-5.(in Chinese with English abstract)
[19] 王辉, 刘志刚, 符世伟. 南海毛蚶形态特征对体重的相关分析[J]. 热带海洋学报, 2007, 26(6): 58-61.WANG H, LIU Z G, FU S W. Correlation between morphological traits and body weight of Scapharca subcrenata from South China Sea[J]. Journal of Tropical Oceanography, 2007, 26(6): 58-61.(in Chinese with English abstract)
[20] 胡贤德, 孙成波, 李镇泉, 等. 北部湾墨吉明对虾和斑节对虾形态性状对体质量的影响分析[J]. 海南大学学报(自然科学版): 2009, 27(1): 48-53.HU X D, SUN C B, LI Z Q, et al. Relation of morphological attributes on body weight for wild Fenneropenaeus merguiensis and Penaeus monodon from Beibu Bay[J]. Natural Science Journal of Hainan University, 2009, 27(1): 48-53.(in Chinese with English abstract)