凤冈锌硒茶对人的肺癌细胞A549增殖的影响分析
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摘要
为了研究凤冈锌硒茶对肺癌A549细胞(人非小细胞肺癌细胞)增殖效应的影响。本实验采取对照试验的方法,将实验分为空白对照组,普通绿茶对照组和凤冈锌硒茶给药组。采用CCK-8和WST-1两种方法检测凤冈锌硒茶提取物对各组A549细胞增殖的抑制情况。实验结果表明:凤冈锌硒茶对A549细胞的增殖具有显著抑制作用,并且呈明显的剂量线性关系。由此我们得出凤冈锌硒茶水提物可以抑制肺癌A549细胞的体外增殖效果强于普通绿茶的结论。
In order to study the effect of zinc selenium tea from Fenggang on the proliferation of lung cancer cell A549, this experiment adopted controlled trials method and set up blank control group, ordinary green tea control group, and zinc selenium tea group. CCK-8 and WST-1 methods were used to detect the inhibiting effect of the extract of Fenggang zinc selenium tea on the proliferation of A549 cell in each group. The experimental results showed that Fenggang zinc selenium tea had significant inhibitory effect on the proliferation of A549 cells, and there was a significant dose linear relationship. Therefore, we concluded that the effect of Fenggang zinc selenium tea on the inhibition of A549 lung cancer cell proliferation in vitro might be stronger than the ordinary green tea.
In order to study the effect of zinc selenium tea from Fenggang on the proliferation of lung cancer cell A549, this experiment adopted controlled trials method and set up blank control group, ordinary green tea control group, and zinc selenium tea group. CCK-8 and WST-1 methods were used to detect the inhibiting effect of the extract of Fenggang zinc selenium tea on the proliferation of A549 cell in each group. The experimental results showed that Fenggang zinc selenium tea had significant inhibitory effect on the proliferation of A549 cells, and there was a significant dose linear relationship. Therefore, we concluded that the effect of Fenggang zinc selenium tea on the inhibition of A549 lung cancer cell proliferation in vitro might be stronger than the ordinary green tea.
引文
Chen W.,Zheng R.,Baade P.D.,Zhang S.,Zeng H.,Bray F.,Je mal A.,Yu X.Q.,and He J.,2016,Cancer statistics in China CA Cancer J.Clin.,66(2):115-132
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Song S.S.,2011,Preliminary study on klotho's transcriptiona regulation,Thesis for M.S.,Third Military Medical Univer sity,Supervisor:Si L.Y.,pp.11-20(宋双双,2011,klotho基因启动子片段转录调控的初步研究,硕士学位论文,第三军医大学,导师:司良毅,pp.11-20)
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Zhang Z.Y.,2011,The investigation of calcitonin gene-related peptide in accelerating fracture-healing in association with traumatic brain injury,Thesis for M.S.,Fourth Military Med ical University,Supervisor:Han Y.S.,pp.21-31(张振宇2011,降钙素基因相关肽在创伤性脑损伤加速骨折愈合中的作用机制研究,硕士学位论文,第四军医大学,导师韩一生,pp.21-31)
Lu W.C.,2012,Reversal of multidrug resistant of hepatocellula carcinoma with combination silent the MDR-1 and MRP-1by SIRNA technique in vitro,Thesis for M.S.,Guangx Medicine University,Supervisor:Pan G.D.,pp.12-21(卢五昌,2012,si RNA联合抑制mrp-1/mdr-1逆转肝细胞癌多药耐药的体外细胞研究,硕士学位论文,广西医科大学导师:潘光栋,pp.12-21)
Song S.S.,2011,Preliminary study on klotho's transcriptiona regulation,Thesis for M.S.,Third Military Medical Univer sity,Supervisor:Si L.Y.,pp.11-20(宋双双,2011,klotho基因启动子片段转录调控的初步研究,硕士学位论文,第三军医大学,导师:司良毅,pp.11-20)
Tian D.Z.,Zhu H.,and Liang Y.J.,2006,Effects and mechanism of curcumin on apoptosis of lung adenocarcinoma A549cells,Zhongguo Linchuang Yaoxue(Chinese Journal of Clini cal Pharmacy),15(1):8-9(天德增,朱辉,梁永杰,2006,姜黄素对肺腺癌A549细胞凋亡的影响及其机制,中国临床药学,15(1):8-9)
Zhang Z.Y.,2011,The investigation of calcitonin gene-related peptide in accelerating fracture-healing in association with traumatic brain injury,Thesis for M.S.,Fourth Military Med ical University,Supervisor:Han Y.S.,pp.21-31(张振宇2011,降钙素基因相关肽在创伤性脑损伤加速骨折愈合中的作用机制研究,硕士学位论文,第四军医大学,导师韩一生,pp.21-31)