凤冈锌硒茶中硒元素在大鼠体内组织中分布研究
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摘要
目的研究灌胃给予大鼠凤冈锌硒茶后硒元素在其体内组织中的分布。方法将42只雄性SD大鼠随机分为7组:空白对照30、90d组,凤冈锌硒茶低、中、高剂量(0.1、0.3、0.6g/kg)30d组,凤冈锌硒茶低剂量(0.1g/kg)90d组,普通绿茶中剂量(0.3g/kg)30d组,每组6只。除空白对照组外,其余组大鼠均连续30d或90d每天灌胃相应茶汤1次。末次给予茶汤24h后将大鼠全部处死,采用石墨炉原子吸收仪测定大鼠肝脏、肾脏、心脏、血浆、脑、胰腺、肺、指甲、肌肉和睾丸中硒含量。结果普通绿茶组大鼠各组织中硒含量与空白对照30d组比较,差异均无统计学意义(P>0.05);与空白对照30d组和普通绿茶组比较,凤冈锌硒茶高剂量30d组大鼠肝脏、肾脏、心脏、胰腺、肺、指甲和肌肉中硒含量显著增加,与空白对照90d组比较,凤冈锌硒茶低剂量90d组大鼠各组织中硒含量均显著增加,差异均有统计学意义(P<0.05或0.01)。结论长期灌胃给予大鼠凤冈锌硒茶能增加大鼠组织中硒元素的含量。
Objective To investigate the distribution of selenium in the tissue of rats after given Fenggang Zinc and Selenium-enriched Tea(ZSTF)by gavage.Methods 42 male SD rats were randomly divided into seven groups:blank control 30,90 d group,ZSTF with low,medium and high-doses(0.1,0.3,0.6 g/kg)30 dgroup,ZSTF with low-dose(0.1 g/kg)90 dgroup,normal green tea with medium-dose(0.3 g/kg)30 dgroup,6 in each group.Except the blank control group,all the rats in the other groups were given tea once a day for 30 dor 90 d.The rats were put to death after 24 hours of tea.Selenium content in liver,kidney,heart,plasma,brain,pancreas,lung,fingernail,muscle and testicles of rats were measured by graphite furnace atomic absorption instrument.Results Selenium content in various tissues of rats in the normal green tea group was compared with that in the blank control group of 30 d,the difference was not statistically significant(P>0.05).Selenium content in liver,kidneys,heart,pancreas,lungs,finger nails and muscles of rats in the high-dose ZSTF group were significantly increased compared with the blank control group 30 dand the normal green tea group,compared with the blank control 90 dgroup,the selenium content in the tissues of rats in the low-dose ZSTF 90 dgroup was significantly increased,the differences were statistically significant(P<0.05 or 0.01).Conclusion The content of selenium in the tissues of rats is increased by long-term gavage of ZSTF.
Objective To investigate the distribution of selenium in the tissue of rats after given Fenggang Zinc and Selenium-enriched Tea(ZSTF)by gavage.Methods 42 male SD rats were randomly divided into seven groups:blank control 30,90 d group,ZSTF with low,medium and high-doses(0.1,0.3,0.6 g/kg)30 dgroup,ZSTF with low-dose(0.1 g/kg)90 dgroup,normal green tea with medium-dose(0.3 g/kg)30 dgroup,6 in each group.Except the blank control group,all the rats in the other groups were given tea once a day for 30 dor 90 d.The rats were put to death after 24 hours of tea.Selenium content in liver,kidney,heart,plasma,brain,pancreas,lung,fingernail,muscle and testicles of rats were measured by graphite furnace atomic absorption instrument.Results Selenium content in various tissues of rats in the normal green tea group was compared with that in the blank control group of 30 d,the difference was not statistically significant(P>0.05).Selenium content in liver,kidneys,heart,pancreas,lungs,finger nails and muscles of rats in the high-dose ZSTF group were significantly increased compared with the blank control group 30 dand the normal green tea group,compared with the blank control 90 dgroup,the selenium content in the tissues of rats in the low-dose ZSTF 90 dgroup was significantly increased,the differences were statistically significant(P<0.05 or 0.01).Conclusion The content of selenium in the tissues of rats is increased by long-term gavage of ZSTF.
引文
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[16]许朋,韩晗,蓝招玲,等.凤冈锌硒茶对人的肺癌细胞A549增殖的影响分析[J].基因组学与应用生物学,2017,36(5):1793-1796.
[17]潘虹,黄琳艳,李义,等.石墨炉原子吸收法测定大鼠饮用富硒绿茶后硒元素在大鼠组织中的含量[J].现代食品科技,2017,33(7):231-237.
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[20]YEH JY,OU BR,GU QP,et al.Influence of gender on selenoprotein W,glutathione peroxidase and Selenium in tissues of rats[J].Comparative Biochem Phys B,1998,119(1):151-155.
[21]RAYMAN MP.Selenium and human health[J].Lancet,2012,379(9822):1256-1268.
[22]刘哲,张峰,梁建斌,等.硒蛋白家族及其功能的研究进展[J].中国兽医杂志,2014,50(7):61-63.
[23]潘利斌,范辉政,蒋建东,等.微量元素硒的体内过程及生物学效应研究进展[J].药学学报,2017,52(12):1849-1858.
[2]王磊,杜菲,孙卉,等.人体硒代谢与硒营养研究进展[J].生物技术进展,2015,5(4):285-290.
[3]张国雄.浅谈硒元素在人体中的作用[J].鄂州大学学报,2005,12(6):67-69.
[4]袁丽君,袁林喜,尹雪斌,等.硒的生理功能,摄入现状与对策研究进展[J].生物技术进展,2016,6(6):396-405.
[5] LAVU RV,VAN DE WIELE T,PRATTI VL,et al.Selenium bioaccessibility in stomach,small intestine and colon:comparison between pure Se compounds,Se-enriched food crops and food supplements[J].Food Chem,2016,197(PtA):382-387.
[6]杨光圻.人体硒需要量和克山病的硒预防[J].实用地方病学杂志,1986,1(3):12-13.
[7]何冠男,武炜,李成会.硒元素的研究进展[J].唐山师范学院学报,2017,39(2):46-48.
[8]周云,刘忠荣.硒与人体健康[J].微量元素与健康研究,2002,19(4):79-80.
[9] NAVARRO-ALARCON M,CABRERA-VIQUE C.Selenium in food and the human body:a review[J].Sci Total Environ,2008,400(1/3):115-141.
[10]黄建国,李双一,谢虹,等.吸烟和缺碘对育龄妇女血硒,血铅和血镉含量的联合作用[J].中国公共卫生,2000,16(11):975-976.
[11]李海蓉,郭亚南,杨林生.长期饮用砖茶人群铅,硒,砷及汞暴露水平[J].卫生研究,2017,46(5):733-736.
[12]潘年松,刘英波,张学愈,等.凤冈锌硒茶中锌硒含量调查与启示[J].中国西部科技,2015,14(1):12.
[13]贵州省质量技术监督局.DB52/489-2005地理标志产品凤冈天然富锌富硒茶[S].贵州省质量技术监督局,2005.
[14]MOLAN AL.Antioxidant and prebiotic activities of selenium-containing green tea[J].Nutrition,2013,29(2):476-477.
[15]胡秋辉,潘根兴,安辛欣,等.富硒茶叶硒的相对生物利用率及其对大鼠吞噬细胞功能的影响[J].南京农业大学学报,2000,23(3):89-92.
[16]许朋,韩晗,蓝招玲,等.凤冈锌硒茶对人的肺癌细胞A549增殖的影响分析[J].基因组学与应用生物学,2017,36(5):1793-1796.
[17]潘虹,黄琳艳,李义,等.石墨炉原子吸收法测定大鼠饮用富硒绿茶后硒元素在大鼠组织中的含量[J].现代食品科技,2017,33(7):231-237.
[18]泡茶小贴士[J].宁波通讯,2015(8):85.
[19]SOHN OS,BLACKWELL L,MATHIS J,et al.Excretion and tissue distribution of selenium following treatment of male F344rats with benzylselenocyanate or sodium selenite[J].Drug Metab Dispos,1991,19(5):865-870.
[20]YEH JY,OU BR,GU QP,et al.Influence of gender on selenoprotein W,glutathione peroxidase and Selenium in tissues of rats[J].Comparative Biochem Phys B,1998,119(1):151-155.
[21]RAYMAN MP.Selenium and human health[J].Lancet,2012,379(9822):1256-1268.
[22]刘哲,张峰,梁建斌,等.硒蛋白家族及其功能的研究进展[J].中国兽医杂志,2014,50(7):61-63.
[23]潘利斌,范辉政,蒋建东,等.微量元素硒的体内过程及生物学效应研究进展[J].药学学报,2017,52(12):1849-1858.