1BL/1RS易位系HMW-GS组成与品质分析
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摘要
为深入挖掘1BL/1RS易位系的品质育种潜力,对收集的300份1BL/1RS易位系的高分子量麦谷蛋白亚基(HMW-GS)组成及其品质性状进行了分析。在300份1BL/1RS易位系 Glu-1位点上共检出12种HMW-GS类型和27种HMW-GS组合,表明这些1BL/1RS易位系具有较高的遗传多样性。在 Glu-A1位点上,亚基Null和1亚基的出现频率分别为47.67%和52.33%; Glu-B1位点有7种等位变异,其中出现频率最高的为7+9亚基对(58.67%); Glu-D1位点有3种等位变异, 2+12亚基对为主要类型,出现频率为71.33%。亚基组合类型中,"Null/7+9/2+12"的出现频率最高(31. 67%)。 Glu-A1、 Glu-B1和 Glu-D1编码优质HMW-GS的比例分别为52.33%、38.34%和16.00%,其中31份1BL/1RS易位系在3个位点均编码优质HMW-GS类型。300份1BL/1RS易位系的品质变异范围较大,有9份材料具有较高的品质育种价值。
In order to further explore the quality breeding potential of 1 BL/1 RS translocation wheat lines,the composition of high-molecular-weight glutenin subunit(HMW-GS)and quality properties of 300 1 BL/1 RS translocation wheat lines were analyzed. There were 12 types of HMW-GS and 27 types of HMW-GS combinations,which indicated that Glu-1 loci had higher level of variation among 1 BL/1 RS translocation lines. The frequencies of HMW-GS Null and 1 at Glu-A1 locus were 47.67% and 52.33%. Seven alleles were identified at Glu-B1 locus,among which 7+9 subunits showed the highest frequency(58.67%). There were three alleles at Glu-D1 locus,among which 2+12 was the most popular allele(71.33%). Null,7+9 and 2+12 presented the highest frequency(31.67%)among all the subunit combinations. The frequencies of prior subunits encoded by Glu-A1, Glu-B1 and Glu-D1 loci,were 52.33%,38.34% and 16.00%,respectively. There were 31 wheat lines carrying good-quality subunits at 3 Glu-1 loci. The quality properties of 300 1 BL/1 RS translocation wheat lines showed distinct differences,among which 9 lines could be utilized in wheat quality breeding.
In order to further explore the quality breeding potential of 1 BL/1 RS translocation wheat lines,the composition of high-molecular-weight glutenin subunit(HMW-GS)and quality properties of 300 1 BL/1 RS translocation wheat lines were analyzed. There were 12 types of HMW-GS and 27 types of HMW-GS combinations,which indicated that Glu-1 loci had higher level of variation among 1 BL/1 RS translocation lines. The frequencies of HMW-GS Null and 1 at Glu-A1 locus were 47.67% and 52.33%. Seven alleles were identified at Glu-B1 locus,among which 7+9 subunits showed the highest frequency(58.67%). There were three alleles at Glu-D1 locus,among which 2+12 was the most popular allele(71.33%). Null,7+9 and 2+12 presented the highest frequency(31.67%)among all the subunit combinations. The frequencies of prior subunits encoded by Glu-A1, Glu-B1 and Glu-D1 loci,were 52.33%,38.34% and 16.00%,respectively. There were 31 wheat lines carrying good-quality subunits at 3 Glu-1 loci. The quality properties of 300 1 BL/1 RS translocation wheat lines showed distinct differences,among which 9 lines could be utilized in wheat quality breeding.
引文
[1]VILLAREAL R L,RAJARM S,MUJEEB-KAZI A,et al.The effect chromosome 1B/1R translocation on the yield potential of certain spring wheats [J].Plant Breeding,1991,106:77.
[2]MORENO-SEVILLE B,BAENZIGER P S,PETERSON C J,et al.The 1B/1R translocation:Agronomic performance of F3-derived lines from a winter wheat cross [J].Crop Science,1995,35:1051.
[3]周阳,何中虎,张改生,等.1BL/1RS易位系小麦在中国小麦育种中的应用[J].作物学报,2004,30(6):531. ZHOU Y,HE Z H,ZHANG G S,et al.Utilization of 1BL/ 1RS translocation in wheat breeding in China [J].Acta Agronomica Sinica,2004,30(6):531.
[4]WIESER H,KIEFFER R,LELLEY T.The influence of 1B/1R chromosome translocation on gluten protein composition and technological properties of bread wheat [J].Journal of the Science of Food and Agriculture,2000,80:1 646.
[5]GRAYBOSCH R A.Uneasy unions:Quality effects of rye chromatin transfers to wheat [J].Journal of Cereal Science,2001,33:7.
[6]刘建军,何中虎,PENA R J,等.1BL/1RS易位对小麦加工品质的影响[J].作物学报,2004,30(2):152. LIU J J,HE Z H,PENA R J,et al.Effect of 1BL/RS translocation on grain quality and noodle quality in bread wheat [J].Acta Agronomica Sinica,2004,30(2):152.
[7]刘丽,阎俊,张艳,等.冬播麦区 Glu-1和 Glu-3位点变异及 1B/1R易位与小麦加工品质性状的关系[J].中国农业科学,2005,38(10):1 945. LIU L,YAN J,ZHANG Y,et al.Allelic variation at the Glu-1 and Glu-3 loci and presence of 1B/1R translocation,and their effects on processing quality in cultivars and advanced lines from autumn-sown wheat regions in China [J].Scientia Agricultura Sinica,2005,38(10):1 945.
[8] CLARKE B C,MUKAI Y,APPELS R.The Sec-1 locus on the short arm of chromosome 1R of rye(Secale cereale)[J].Chromosoma,1996,105:269.
[9]GRAYBOSCH R A,PETERSON C J,SHELTON D R,et al.Genotypic and environmental modification of wheat flour protein composition in relation to end-use quality [J].Crop Science,1996,36:298.
[10]MARTIN P,GOMEZ M,CARRILLO J M.Interaction between allelic variation at the Glu-D1 locus and a 1BL/1RS translocation on flour quality in bread wheat [J].Crop Science,2001,41:1080.
[11] 柴建芳,王海波,马秀英,等.ω-黑麦碱基因沉默对小麦1B/1R易位系加工品质的影响 [J].作物学报,2016,42(5):630. CHAI J F,WANG H B,MA X Y,et al.Effect of ω-secalin gene silencing on processing quality of wheat 1B/1R translocation line [J].Acta Agronomica Sinica,2016,42(5):630.
[12] FRANCIS H A,LEITCH A R,KOEBNER R M.Conversion of a RAPD-generated PCR product,containing a novel dispersed repetitive element,into a fast and robust assay for the presence of rye chromatin in wheat [J].Theoretical and Applied Genetics,1995,90:638.
[13]VANCAMPENHOUT S,VANDERSTAPPEN J,SAGI L,et al.Locus specific primers for LMW glutenin genes on each of the group 1 chromosomes of hexaploid wheat [J].Theoretical and Applied Genetics,1995,91(2):317.
[14]魏学军,张娜,胡亚亚,等.53个小麦品种中1BL/1RS易位系的分子检测[J].分子植物育种,2013,11(4):505. WEI X J,ZHANG N,HU Y Y,et al.Detection of 1BL/1RS by molecular markers in 53 wheat varieties [J].Molecular Plant Breeding,2013,11(4):505.
[15]魏乐,刘琦,刘宝龙,等.无芒小麦高分子量麦谷蛋白亚基组成分析[J].西北农业学报,2011,20(6):85. WEI L,LIU Q,LIU B L,et al.Diversity of HMW-GS composition in 100 beardless spring wheat cultivars [J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(6):85.
[16]朱金宝,刘广田,张树棒.小麦籽粒高、低分子量谷蛋白亚基及其与品质关系的研究[J].中国农业科学,1996,29(1):34. ZHU J B,LIU G T,ZHANG S B.High and low molecular subunits of glutenin and their relationships with wheat quality [J].Scientia Agricultura Sinica,1996,29(1):34.
[17]MOREL M H.Acid-polyacrylamide gel electrophoresis of wheat glutenins:A new tool for the separation of high-and low-molecular-weight subunits [J].Cereal Chemistry,1994,71:238.
[18]PAYNE P I,LAWRENCE G I.Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1, Glu-D1 which code for high molecular weight glutenin subunits in hexaploid wheat [J].Cereal Research Communications,1983,11:30.
[19]徐一如,刘翠玲,孙晓荣,等.基于近红外和中红外光谱技术的小麦粉品质检测及掺杂鉴别方法[J].食品科学,2014,35(12):129. XU Y R,LIU C L,SUN X R,et al.Determination of wheat flour adulteration based on near-and mid-infrared spectroscopy [J].Food Science,2014,35(12):129.
[20]赖立群,麻旺琼,陈华才.近红外漫反射光谱结合神经网络检测小麦蛋白质含量[J].中国计量学院学报,2015,26(1):57. LAI L Q,MA W Q,CHEN H C.Wheat protein nondestructive analysis with near infrared reflectance spectroscopy combined with artificial neutral networks [J].Journal of China University of Metrology,2015,26(1):57.
[21] FRIEBE B,HEUN M,BUSHUK W.Cytological characterization,powdery mildew resistance and storage protein composition of tetraploid and hexaploid 1BL/1RS wheat-rye translocation lines [J].Theoretical and Applied Genetics,1989(78):428.
[22]张勇,申小勇,张文祥,等.高分子量麦谷蛋白5+10亚基和1BL/1RS易位分子标记辅助选择在小麦品种育种中的应用[J].作物学报,2012,38(10):1749. ZHANG Y,SHEN X Y,ZHANG W X,et al.Marker assisted selection of HMW-glutenin 1Dx5+1Dy10 gene and 1B/1R translocation for improving industry quality in common wheat [J].Acta Agronomica Sinica,2012,38(10):1749.
[23]PAYNE P I,NIGHTINGALE M A,KRATTIGER A F,et al.The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties [J].Journal of the Science of Food and Agriculture,1987,40(1):54.
[24] BRNNEKE V,ZIMMERMANN G,KILLERMANN B.Effect of high molecular weight glutenins and D-zone gliadins on bread-making quality in Germany wheat varieties [J].Cereal Research Communications,2000,28:187.
[25] BRANLARD G,DARDEVET M,SACCOMANO R,et al.Genetic diversity of wheat storage proteins and bread wheat quality [J].Euphytica,2001,119:59.
[26]SOZINOV A A,NOVOSELSKAYA A,LUSHNIKOVA A A,et al.Cytological and biochemical analysis of breed wheat variants with 1B/1R substitutions and translocations in the karayotype(in Russian)[J].Tsitologiya I Genetika,1987,21(4):256.
[27] 宋建民,刘爱峰,吴祥云,等.高分子量谷蛋白亚基组成及其含量与小麦品质关系研究[J].中国农业科学,2003,36(2):131. SONG J M,LIU A F,WU X Y,et al.Composition and content of high-molecular-weight glutenin subunits and their relations with wheat quality [J].Scientia Agricultura Sinica,2003,36(2):131.
[2]MORENO-SEVILLE B,BAENZIGER P S,PETERSON C J,et al.The 1B/1R translocation:Agronomic performance of F3-derived lines from a winter wheat cross [J].Crop Science,1995,35:1051.
[3]周阳,何中虎,张改生,等.1BL/1RS易位系小麦在中国小麦育种中的应用[J].作物学报,2004,30(6):531. ZHOU Y,HE Z H,ZHANG G S,et al.Utilization of 1BL/ 1RS translocation in wheat breeding in China [J].Acta Agronomica Sinica,2004,30(6):531.
[4]WIESER H,KIEFFER R,LELLEY T.The influence of 1B/1R chromosome translocation on gluten protein composition and technological properties of bread wheat [J].Journal of the Science of Food and Agriculture,2000,80:1 646.
[5]GRAYBOSCH R A.Uneasy unions:Quality effects of rye chromatin transfers to wheat [J].Journal of Cereal Science,2001,33:7.
[6]刘建军,何中虎,PENA R J,等.1BL/1RS易位对小麦加工品质的影响[J].作物学报,2004,30(2):152. LIU J J,HE Z H,PENA R J,et al.Effect of 1BL/RS translocation on grain quality and noodle quality in bread wheat [J].Acta Agronomica Sinica,2004,30(2):152.
[7]刘丽,阎俊,张艳,等.冬播麦区 Glu-1和 Glu-3位点变异及 1B/1R易位与小麦加工品质性状的关系[J].中国农业科学,2005,38(10):1 945. LIU L,YAN J,ZHANG Y,et al.Allelic variation at the Glu-1 and Glu-3 loci and presence of 1B/1R translocation,and their effects on processing quality in cultivars and advanced lines from autumn-sown wheat regions in China [J].Scientia Agricultura Sinica,2005,38(10):1 945.
[8] CLARKE B C,MUKAI Y,APPELS R.The Sec-1 locus on the short arm of chromosome 1R of rye(Secale cereale)[J].Chromosoma,1996,105:269.
[9]GRAYBOSCH R A,PETERSON C J,SHELTON D R,et al.Genotypic and environmental modification of wheat flour protein composition in relation to end-use quality [J].Crop Science,1996,36:298.
[10]MARTIN P,GOMEZ M,CARRILLO J M.Interaction between allelic variation at the Glu-D1 locus and a 1BL/1RS translocation on flour quality in bread wheat [J].Crop Science,2001,41:1080.
[11] 柴建芳,王海波,马秀英,等.ω-黑麦碱基因沉默对小麦1B/1R易位系加工品质的影响 [J].作物学报,2016,42(5):630. CHAI J F,WANG H B,MA X Y,et al.Effect of ω-secalin gene silencing on processing quality of wheat 1B/1R translocation line [J].Acta Agronomica Sinica,2016,42(5):630.
[12] FRANCIS H A,LEITCH A R,KOEBNER R M.Conversion of a RAPD-generated PCR product,containing a novel dispersed repetitive element,into a fast and robust assay for the presence of rye chromatin in wheat [J].Theoretical and Applied Genetics,1995,90:638.
[13]VANCAMPENHOUT S,VANDERSTAPPEN J,SAGI L,et al.Locus specific primers for LMW glutenin genes on each of the group 1 chromosomes of hexaploid wheat [J].Theoretical and Applied Genetics,1995,91(2):317.
[14]魏学军,张娜,胡亚亚,等.53个小麦品种中1BL/1RS易位系的分子检测[J].分子植物育种,2013,11(4):505. WEI X J,ZHANG N,HU Y Y,et al.Detection of 1BL/1RS by molecular markers in 53 wheat varieties [J].Molecular Plant Breeding,2013,11(4):505.
[15]魏乐,刘琦,刘宝龙,等.无芒小麦高分子量麦谷蛋白亚基组成分析[J].西北农业学报,2011,20(6):85. WEI L,LIU Q,LIU B L,et al.Diversity of HMW-GS composition in 100 beardless spring wheat cultivars [J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(6):85.
[16]朱金宝,刘广田,张树棒.小麦籽粒高、低分子量谷蛋白亚基及其与品质关系的研究[J].中国农业科学,1996,29(1):34. ZHU J B,LIU G T,ZHANG S B.High and low molecular subunits of glutenin and their relationships with wheat quality [J].Scientia Agricultura Sinica,1996,29(1):34.
[17]MOREL M H.Acid-polyacrylamide gel electrophoresis of wheat glutenins:A new tool for the separation of high-and low-molecular-weight subunits [J].Cereal Chemistry,1994,71:238.
[18]PAYNE P I,LAWRENCE G I.Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1, Glu-D1 which code for high molecular weight glutenin subunits in hexaploid wheat [J].Cereal Research Communications,1983,11:30.
[19]徐一如,刘翠玲,孙晓荣,等.基于近红外和中红外光谱技术的小麦粉品质检测及掺杂鉴别方法[J].食品科学,2014,35(12):129. XU Y R,LIU C L,SUN X R,et al.Determination of wheat flour adulteration based on near-and mid-infrared spectroscopy [J].Food Science,2014,35(12):129.
[20]赖立群,麻旺琼,陈华才.近红外漫反射光谱结合神经网络检测小麦蛋白质含量[J].中国计量学院学报,2015,26(1):57. LAI L Q,MA W Q,CHEN H C.Wheat protein nondestructive analysis with near infrared reflectance spectroscopy combined with artificial neutral networks [J].Journal of China University of Metrology,2015,26(1):57.
[21] FRIEBE B,HEUN M,BUSHUK W.Cytological characterization,powdery mildew resistance and storage protein composition of tetraploid and hexaploid 1BL/1RS wheat-rye translocation lines [J].Theoretical and Applied Genetics,1989(78):428.
[22]张勇,申小勇,张文祥,等.高分子量麦谷蛋白5+10亚基和1BL/1RS易位分子标记辅助选择在小麦品种育种中的应用[J].作物学报,2012,38(10):1749. ZHANG Y,SHEN X Y,ZHANG W X,et al.Marker assisted selection of HMW-glutenin 1Dx5+1Dy10 gene and 1B/1R translocation for improving industry quality in common wheat [J].Acta Agronomica Sinica,2012,38(10):1749.
[23]PAYNE P I,NIGHTINGALE M A,KRATTIGER A F,et al.The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties [J].Journal of the Science of Food and Agriculture,1987,40(1):54.
[24] BRNNEKE V,ZIMMERMANN G,KILLERMANN B.Effect of high molecular weight glutenins and D-zone gliadins on bread-making quality in Germany wheat varieties [J].Cereal Research Communications,2000,28:187.
[25] BRANLARD G,DARDEVET M,SACCOMANO R,et al.Genetic diversity of wheat storage proteins and bread wheat quality [J].Euphytica,2001,119:59.
[26]SOZINOV A A,NOVOSELSKAYA A,LUSHNIKOVA A A,et al.Cytological and biochemical analysis of breed wheat variants with 1B/1R substitutions and translocations in the karayotype(in Russian)[J].Tsitologiya I Genetika,1987,21(4):256.
[27] 宋建民,刘爱峰,吴祥云,等.高分子量谷蛋白亚基组成及其含量与小麦品质关系研究[J].中国农业科学,2003,36(2):131. SONG J M,LIU A F,WU X Y,et al.Composition and content of high-molecular-weight glutenin subunits and their relations with wheat quality [J].Scientia Agricultura Sinica,2003,36(2):131.