工作场所空气中六价铬的离子色谱-柱后衍生-分光光度测定法
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摘要
目的建立工作场所空气中六价铬的离子色谱-柱后衍生-分光光度测定法。方法使用携带式采集器和PVC滤膜进行空气样品采集,样品经碳酸氢钠溶液洗脱后,以硫酸铵和氨水混合溶液为流动相,二苯羰酰二肼为衍生化试剂,用离子色谱-柱后衍生-分光光度法进行测定。结果该方法的线性范围为1~1 000μg/L,仪器检出限为0.07μg/L,加标回收率为97.2%~103.5%,RSD为0.93%~1.94%。采集960 L工作场所空气时,最低检出浓度为0.7 ng/m3。结论该方法检出限低,精密度好,适用于工作场所空气中的六价铬测定。
Objective To develop a method for the determination of hexavalent chromium in workplace air. Methods The air samples were collected by the personal air samplers with PVC filter membranes and were eluted by solution of sodium bicarbonate. The content of hexavalent chromium in the extract was determined by ion chromatography coupled with post-column derivatization with UV/visible absorption detection, which was applied with a mixture of ammonium sulfate and ammonia as the mobile phase and the diphenylcarbazide solution as the post-column derivatizing reagent. Resluts The results showed a good linear in the range of 1-1 000 μg/L. The limit of detection was 0.07 μg/L. The recovery rate of the samples was in the range of 97.2% to 103.5% and the relative standard deviations were from 0.93% to 1.94%. The lowest detection concentration was 0.7 ng/m3 based on 960 L air sampling volume. Conclusion The developed method is featured by low detection limit and high precision and is applicable to the determination of hexavalent chromium in workplace air.
Objective To develop a method for the determination of hexavalent chromium in workplace air. Methods The air samples were collected by the personal air samplers with PVC filter membranes and were eluted by solution of sodium bicarbonate. The content of hexavalent chromium in the extract was determined by ion chromatography coupled with post-column derivatization with UV/visible absorption detection, which was applied with a mixture of ammonium sulfate and ammonia as the mobile phase and the diphenylcarbazide solution as the post-column derivatizing reagent. Resluts The results showed a good linear in the range of 1-1 000 μg/L. The limit of detection was 0.07 μg/L. The recovery rate of the samples was in the range of 97.2% to 103.5% and the relative standard deviations were from 0.93% to 1.94%. The lowest detection concentration was 0.7 ng/m3 based on 960 L air sampling volume. Conclusion The developed method is featured by low detection limit and high precision and is applicable to the determination of hexavalent chromium in workplace air.
引文
[1]冉文婧,王永义.职业性铬鼻病[J].中国工业医学杂志,2013,26(5):357-359.
[2]方利强,秦光明.低剂量铬暴露工人铬鼻病调查分析[J].环境与职业医学,2016,33(2):160-162.
[3]U.S.Department of Health and Human Services,Centers for Disease Control and Prevention,National Institute for Occupational Safety and Health.Evaluation of skin and respiratory symptoms among employees with exposure to metals,metalworking fluids,and noise at an orthopedic implant manufacturer[R].NIOSH HHE Report,2013:33-38.
[4]NIOSH Manual of Analytical Methods(NMAM).Hexavalent chromium by ion chromatography,Method 7065[S].Issue 1,2003-03-15.
[5]朱富强,李月红,贾会美,等.离子色谱法测定工作场所空气中六价铬含量[J].理化检验:化学分册,2017,53(2):165-168.
[6]刀谞,张霖琳,李丽和,等.离子色谱-柱后衍生-紫外可见检测法测定大气颗粒物(PM2.5、PM10)中的六价铬[J].环境化学,2014,33(7):1194-1200.
[2]方利强,秦光明.低剂量铬暴露工人铬鼻病调查分析[J].环境与职业医学,2016,33(2):160-162.
[3]U.S.Department of Health and Human Services,Centers for Disease Control and Prevention,National Institute for Occupational Safety and Health.Evaluation of skin and respiratory symptoms among employees with exposure to metals,metalworking fluids,and noise at an orthopedic implant manufacturer[R].NIOSH HHE Report,2013:33-38.
[4]NIOSH Manual of Analytical Methods(NMAM).Hexavalent chromium by ion chromatography,Method 7065[S].Issue 1,2003-03-15.
[5]朱富强,李月红,贾会美,等.离子色谱法测定工作场所空气中六价铬含量[J].理化检验:化学分册,2017,53(2):165-168.
[6]刀谞,张霖琳,李丽和,等.离子色谱-柱后衍生-紫外可见检测法测定大气颗粒物(PM2.5、PM10)中的六价铬[J].环境化学,2014,33(7):1194-1200.