姜黄薄壁细胞原位拉曼光谱研究
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摘要
用拉曼光谱原位分析姜黄橙黄色及黄色薄壁细胞中的物质。德宏、广西及西双版纳姜黄三种样品橙黄色细胞的拉曼光谱非常相似,较强峰出现在1 632/1 633/1 637、1 599/1 601/1 605、1 184/1 186/1 190 cm-1,中等强度的峰出现在1 529/1 528/1 534、1 425/1 426/1 430、1 306/1 308/1 311、1 235/1 235/1 239、1 167/1 168/1 173、1 122/1 125/1 130、967/969/975 cm-1。三种姜黄薄壁细胞中出现的强峰、次强峰位置、峰型都一致,说明三种姜黄橙色薄壁细胞中物质的主要成分相同。与姜黄素(curcumin)拉曼光谱的主要的19条谱线比较,在姜黄橙色细胞拉曼谱的17条谱峰中有16条与之对应。而黄色薄壁细胞中大部分谱线与支链淀粉的谱峰有较好的对应关系。用密度泛函理论计算了姜黄素的拉曼光谱,并对谱线进行了初步的归属。
In order to identify the ingredients in parenchymatous cells at room temperature,and avoid sample pretreatment and extractions which can be labor intensive,A method was presented that a novel and original approach to analyze in situ the main components of the parenchymatous cells in Curcuma longa L by means of Raman spectroscopy. Fresh samples were prepared by free-hand section. Under the DXR laser confocal micro Raman spectrometer,the parenchymatous cells can be seen with objective lens of 10. Dehong,guangxi,xisuabannan of Curcuma longa L,the three sample have similar Raman spectrum,high intensity bands are present at 1 632/1 633/1 637、1 599/1 601/1 605、1 184/1 186/1 190 cm-1,middle intensity bands are present at 1 529/1 528/1 534、1 425/1 426/1 430、1 306/1 308/1 311、1 235/1 235/1 239、1 167/1 168/1 173、1 122/1 125/1 130、967/969/975. It means that the three samples have the same principal component. It has been found that the Raman spectrum of three sample correlate very well with curcumin Raman spectrum. There are 19 main spectroscopic bands in the Raman spectrum of curcumin. For the 17 presented spectroscopic bands of Curcuma longa L orange parenchymatous cells,there are 16 bands which correlate very well with curcumin. For Curcuma longa L yellow parenchymatous cells most bands correlate very well with amylopectin. The Raman spectroscopy of curcumin was calculated by density functional theory(DFT). The Raman spectroscopic bands were given assignment with the help of calculation.
In order to identify the ingredients in parenchymatous cells at room temperature,and avoid sample pretreatment and extractions which can be labor intensive,A method was presented that a novel and original approach to analyze in situ the main components of the parenchymatous cells in Curcuma longa L by means of Raman spectroscopy. Fresh samples were prepared by free-hand section. Under the DXR laser confocal micro Raman spectrometer,the parenchymatous cells can be seen with objective lens of 10. Dehong,guangxi,xisuabannan of Curcuma longa L,the three sample have similar Raman spectrum,high intensity bands are present at 1 632/1 633/1 637、1 599/1 601/1 605、1 184/1 186/1 190 cm-1,middle intensity bands are present at 1 529/1 528/1 534、1 425/1 426/1 430、1 306/1 308/1 311、1 235/1 235/1 239、1 167/1 168/1 173、1 122/1 125/1 130、967/969/975. It means that the three samples have the same principal component. It has been found that the Raman spectrum of three sample correlate very well with curcumin Raman spectrum. There are 19 main spectroscopic bands in the Raman spectrum of curcumin. For the 17 presented spectroscopic bands of Curcuma longa L orange parenchymatous cells,there are 16 bands which correlate very well with curcumin. For Curcuma longa L yellow parenchymatous cells most bands correlate very well with amylopectin. The Raman spectroscopy of curcumin was calculated by density functional theory(DFT). The Raman spectroscopic bands were given assignment with the help of calculation.
引文
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[19]司民真,张德清,李伦,等.姜油细胞原位拉曼光谱研究[J].光谱学与光谱分析,2016,36(11):3578-3581.SI Minzhen,ZHANG Deqing,LI Lun,et al. In situ research on Ginger oil cell with Raman[J]. Spectroscopy and Spectral Analysis,2016,36(11):3578-3581.
[20] PARKER F S. Applications of infrared,Raman and resonance Raman spectroscopy in biochemistry[M]. New York:Plenum Press,1984,
[2] JUREN KA J S. Anti-inflammatory properties of curcumin,a major constituent of Curcuma longa:a review of preclinical and clinical research[J]. Alternative Medicine Review,2009,14(2):141-153.
[3] DUAN W,CHANG Y,LI R,et al. Curcumin inhibits hypoxia inducible factor-1α-induced epithelial-mesenchymal transition in HepG2 hepatocellular carcinoma cells[J]. Molecular Medicine Reports,2014,10(5):2505-2510.
[4] MUTHU S,GRISHMA R,M ADHU,et al. The multifaceted role of curcumin in cancer prevention and treatment[J]. Molecules,2015,20(2):2728-2769.
[5] XU M. Curcum in suppresses proliferation and induces apoptosis of human hepatocellular carcinoma cells via the wnt signaling pathway[J]. International Journal of Oncology,2013,43(6):1951-1959.
[6] BABAEI E,SADEGHIZADEH M,HASSAN Z M,et al.Dendrosomal curcumin significantly suppresses cancer cell proliferation in vitro and in vivo[J]. Int Immuno Pharmacol,2012,12(1):226-234.
[7] BALWANT R,JASDEEP K,M ARIA C. Anti-oxidation actions of curcumin in two forms of bed rest:oxidative stress serum and salivary markers[J]. Asian Pacifc J Trop,2010,3(8):651-654.
[8] KIZHAKKEDATH R. Clinical evaluation of a formulation containing Curcuma longa and Boswellia serrata extracts in the management of knee osteoarthritis[J]. Mol Med Rep, 2013, 8(5):1542-1548.
[9] POTTER P E. Curcumin:a natural substance with potential efficacy in Alzheimer’s disease[J]. J Exp Pharmacol,2013,5:23-31.
[10] SRIVATAVA R M,SINGH S,DUBEY S K,et al. Immunomodulatory and therapeutic activity of curcumin[J]. Int Immunopharmacol,2011,11(6):331-341.
[11]史晶晶,冯素香,郝蕊,等.姜黄提取物中姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素的含量测定[J].中医学报,2015,30(205):853-855.SHI Jingjing,FENG Shuxiang,HAO Rui,et al. Demethoxycurucumin and bisdemethoxycurucumin in curcuma longa extractive[J]. China Journal of Chinese medicine, 2015, 30(205):853-855.
[12]张军,石典花.四种不同药材来源郁金饮片中吉马酮和姜黄素含量的高效液相色谱法测定[J].时珍国医国药,2016,27(8):1846-1849.ZHANG Jun,SHI Dianhua. Determination the contents of germacrone and curcumin in four different sources of Curcuma radix pieces by HPLC[J]. Lishizhen Medicine and Materia Medica Research,2016,27(8):1846-1849.
[13]白文莉,金燕. HPLC法测定姜黄提取物中有效成分含量[J].中国药师,2014,(6):1050-1052.BAI Wenli,JIN Yan. Determination of active in gredients in extracts of Curcuma longa L by HPLC[J]. China Pharmacist,2014,(6):1050-1052.
[14]高苏亚,王黎,范涛,等.区带毛细管电泳法测定郁金银屑片中的姜黄素[J].光谱实验室,2012,29(6):3496-3499.GAO Suya,WANG Li,FAN Tao,et al. Determination of curcum in yujinyinxie tablets by capillary zone electrophoresis[J].Chinese Journal of Spectroscopy Laboratory,2012,29(6):3496-3499.
[15]徐仲溪,王坤波.姜黄素的高效薄层色谱分析[J].食品科学,2004,25(6):156-159.XU Zhongxi,WANG Sunbo. Analysis of curcumin by high performance thin-layer chromatoraphy(HPTLC)[J]. Food Science,2004,25(6):156-159.
[16]司民真,张德清,李伦,等.姜科植物长柄山姜及茴香砂仁精油原位拉曼光谱研究[J].光谱学与光谱分析,2018,38(2):448-453.SI Minzhen,ZHANG Deqing,LI Lun,et al. Essential oils of Zingiberaceae Alpinia kwangsiensis and Achasma yunnanensis in situ research with Raman[J]. Spectroscopy and Spectral Analysis,2018,38(2):448-453.
[17]司民真,李伦,张川云,等.大高良姜与节鞭山姜油细胞原位拉曼光谱研究[J].光散射学报,2017,29(03):239-242.SI Minzhen,LI Lun,ZHANG Chuanyun,et al. In situ research on Alpinia galanga(L.)Will and Alpinia conchigera Griff oil cell with Raman[J]. The Journal of Light Scattering,2017,29(3):239-242.
[18]司民真,李伦,张川云,等.毛姜花油细胞原位拉曼光谱研究[J].激光生物学报,2017,26(4):298-302.SI Minzhen,LI Lun,ZHANG Chuanyun,et al. In situ research on Hedychium villosum Wall oil cell with Raman[J]. Acta Laser Biology Sinica,2017,26(4):298-302.
[19]司民真,张德清,李伦,等.姜油细胞原位拉曼光谱研究[J].光谱学与光谱分析,2016,36(11):3578-3581.SI Minzhen,ZHANG Deqing,LI Lun,et al. In situ research on Ginger oil cell with Raman[J]. Spectroscopy and Spectral Analysis,2016,36(11):3578-3581.
[20] PARKER F S. Applications of infrared,Raman and resonance Raman spectroscopy in biochemistry[M]. New York:Plenum Press,1984,