20株食源性科瓦利斯沙门菌药物敏感试验和分子分型分析
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摘要
目的了解2016年河南省食源性沙门菌优势血清型(科瓦利斯沙门菌)药物敏感性和分子分型特征。方法食品中沙门菌的检测和血清分型参照2016年国家食品污染物和有害因素风险监测工作手册、脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)分子分型分析和药物敏感试验参照2016年食源性疾病监测工作手册和国家食源性疾病分子溯源网络(TraNetChina)使用手册。结果采用微量最低抑菌浓度法对20株食源性科瓦利斯沙门菌进行15种抗生素的药物敏感试验,20株菌对四环素、红霉素100%耐药,对环丙沙星、萘啶酸耐药率分别为80.00%、45.00%,对头孢西丁、头孢唑啉产生中度耐药,中度耐药率分别为15.00%、10.00%,对头孢噻圬、头孢他啶、亚胺培南、庆大霉素、氨苄西林、氨苄西林/舒巴坦、氯霉素、甲氧苄氨嘧啶/磺胺甲噁唑、阿奇霉素全部敏感。有8株菌出现多重耐药现象,最多对4类4种抗生素产生耐药。20株科瓦利斯沙门菌用XbaⅠ酶切、经脉冲场凝胶电泳后,获得9种带型,每种带型包括1～6株菌,相似度61.7%～100%,以SCVX01.HA0008和SCVX01.HA0009为主要优势带型。结论河南省食源性科瓦利斯沙门菌对第三代头孢类抗生素全部敏感,对喹诺酮类和氟喹诺酮类出现不同程度耐药,且带型存在地区聚集性提示存在共同来源或者交叉污染的情况,相关部门应加强监测,提早防范。
Objective To understand the drug sensitivity and molecular typing characteristics of the dominant serotype of foodborne Salmonella(Salmonella Corvallis) in Henan province in 2016. Methods Detection and serotyping of Salmonella in foods refered to the 2016 national food contaminants and hazardous factors risk monitoring workbook. Pulsed field gel electrophoresis(PFGE) molecular typing analysis and drug susceptibility testing refered to the 2016 foodborne disease surveillance workbook and the national foodborne diseases tracing network(TraNet China) user manual. Results Drug sensitivity test of 15 antibiotics against 20 strains of foodborne Salmonella was carried out by micro-minimum inhibitory concentration method, 20 strains were 100% resistant to tetracycline and erythromycin, and the resistance rates to ciprofloxacin and nalidixic acid were 80.00% and 45.00%, respectively. They were moderately resistant to cefoxitin and cefazolin, with the resistance rates of 15.00% and 10.00%, and all were sensitive to cefotaxime, ceftazidime, imipenem, gentamicin, ampicillin, ampicillin/sulbactam, chloramphenicol, trimethoprim/sulfamethoxazole, and azithromycin. Totally 8 strains showed multi-drug resistance and were resistant to 4 types of 4 antibiotics, and 20 Salmonella Corvallis strains were disposed with XbaI and pulsed gel electrophoresis to obtain 9 band types. Each band type included 1 to 6 strains, the similarity were 61.7%-100%, and SCVX01.HA0008 and SCVX01.HA0009 were the main dominant belt types. Conclusion Foodborne Salmonella Corvallis in Henan province is all sensitive to the third-generation cephalosporin antibiotics, and has different degrees of resistance to quinolones and fluoroquinolones, and the regional aggregation of the bands indicates that there is a common source or cross-contamination. Relevant departments should strengthen monitoring and prevent early.
Objective To understand the drug sensitivity and molecular typing characteristics of the dominant serotype of foodborne Salmonella(Salmonella Corvallis) in Henan province in 2016. Methods Detection and serotyping of Salmonella in foods refered to the 2016 national food contaminants and hazardous factors risk monitoring workbook. Pulsed field gel electrophoresis(PFGE) molecular typing analysis and drug susceptibility testing refered to the 2016 foodborne disease surveillance workbook and the national foodborne diseases tracing network(TraNet China) user manual. Results Drug sensitivity test of 15 antibiotics against 20 strains of foodborne Salmonella was carried out by micro-minimum inhibitory concentration method, 20 strains were 100% resistant to tetracycline and erythromycin, and the resistance rates to ciprofloxacin and nalidixic acid were 80.00% and 45.00%, respectively. They were moderately resistant to cefoxitin and cefazolin, with the resistance rates of 15.00% and 10.00%, and all were sensitive to cefotaxime, ceftazidime, imipenem, gentamicin, ampicillin, ampicillin/sulbactam, chloramphenicol, trimethoprim/sulfamethoxazole, and azithromycin. Totally 8 strains showed multi-drug resistance and were resistant to 4 types of 4 antibiotics, and 20 Salmonella Corvallis strains were disposed with XbaI and pulsed gel electrophoresis to obtain 9 band types. Each band type included 1 to 6 strains, the similarity were 61.7%-100%, and SCVX01.HA0008 and SCVX01.HA0009 were the main dominant belt types. Conclusion Foodborne Salmonella Corvallis in Henan province is all sensitive to the third-generation cephalosporin antibiotics, and has different degrees of resistance to quinolones and fluoroquinolones, and the regional aggregation of the bands indicates that there is a common source or cross-contamination. Relevant departments should strengthen monitoring and prevent early.
引文
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[14]张东方,袁飞,陈颖,等.沙门菌分子分型方法研究进展[J].中国公共卫生,2012,28(1):117-120.Zhang DF,Yuan F,Chen Y,et al.Research progress on molecular typing of Salmonella[J].Chin J Public Health,2012,28(1):117-120.
[15]游兴勇,刘成伟,朱应飞,等.江西省食源性沙门菌血清分型及脉冲场凝胶电泳指纹图谱研究[J].中国食品卫生杂志,2014,26(6):528-532.You XY,Liu CW,Zhu YF,et al.Serotyping and PFGE type of Salmonella isolates from food in Jiangxi province[J].Chin J Food Hyg,2014,26(6):528-532.
[16]管峰,杨季芳.食源性致病菌溯源分型技术研究进展[J].浙江万里学院学报,2012,25(4):86-90.Guan F,Yang JF.Progress in research on anadromous differentiation of edible pathogens[J].J Zhejiang Wanli Univ,2012,25(4):86-90.
[17]崔志刚,王爱敏,王鸣柳,等.婴幼儿腹泻感染的非伤寒沙门菌分子分型和耐药情况研究[J].疾病监测,2014,29(6):428-431.Cui ZG,Wang AM,Wang ML,et al.Molecular subtyping and antibiotic resistance of non-typhi Salmonella isolated from infants[J].Dis Surveill,2014,29(6):428-431.
[18]谢晓红,赵宏伟,陈洪友,等.上海市奉贤区188株沙门菌分子分型研究[J].预防医学情报杂志,2018,34(11):1391-1395.Xie XH,Zhao HW,Chen HY,et al.Study on the molecular typing of 188Salmonella strains in fengxian district of Shanghai[J].J Prev Med Inf,2018,34(11):1391-1395.
[19]耿英芝,李飞,于淼,等,2016-2017年辽宁省沙门菌耐药菌谱与分子分型[J].中国微生态学杂志,2018,30(10):1142-1146.Geng YZ,Li F,Yu M,et al.Characteristics of drug resistance and molecular typing of Salmonella isolated in Liaoning,2016-2017[J].Chin J Microecol,2018,30(10):1142-1146.
[20]Long DL,Yan MY,Cui ZG,et al.Antimicrobial resistance research of non-typhoidal Salmonella enteric infections in four hospitals in Shenzhen,2012-2016[J].Chin J Prevent Med,2017,51(12):1118-1121.
[21]Andreoli G,Merla C,Valle CD,et al.Foodborne salmonellosis in Italy:Characterization of Salmonella enterica serovar typhimurium and monophasic variant 4,[5],12:i-isolated from salami and human patients[J].J Food Prot,2017,80(4):632-639.
[2]Scallan E,Robert MH,Frederick JA,et al.Foodborne illness acquired in the United States:Major pathogens.Emerg[J].Infect Dis,2011,(17):7-15.
[3]穆玉娇,张白帆,赵嘉咏,等.河南省腹泻患者沙门菌血清型及其耐药性变迁研究[J].中国卫生检验杂志,2016,26(9):1335-1338.Mu YJ,Zhang BF,Zhao JY,et al.Study on the changes of Salmonella serotypes and drug resistance in patients with diarrhea in Henan[J].Chin JHealth Lab Technol,2016,26(9):1335-1338.
[4]杨小娟,吴清平,张菊梅,等.广东部分零售畜禽产品沙门菌生化型和血清型分析[J].卫生研究,2013,42(4):615-618.Yang XJ,Wu QP,Zhang JM,et al.Biochemical and serological characterization Salmonella from retail meats in Guangdong province[J].J Hyg Res,2013,42(4):615-618.
[5]李映霞,余晓娜,孙凤琪,等.国内首次从健康人群体检中检出一株科瓦利斯沙门菌[J].中国卫生检验杂志,2010,20(6):1567.Li YX,Yu XN,Sun FQ,et al.A Corvallis Salmonella was detected firstly in medical examination of healthy people in China[J].Chin J Health Lab Technol,2010,20(6):1567.
[6]黄焕宜,许小婵,唐金龙,等.血液中分离出罕见的科瓦利斯沙门菌[J].中华检验医学杂志,2007,30(8):862.Huang HY,Xu XC,Tang JL,et al.The rare Corvallis Salmonella isolated in the blood[J].Chin J Lab Med,2007,30(8):862.
[7]刘杰,杨如璞,黄淑华,等.河南省首次发现的科瓦利斯沙门菌的鉴定与探讨[J].中国卫生检验杂志,2013,23(2):278-280.Liu J,Yang RP,Huang SH,et al.The identification and analysis of the first found Salmonella corvallis in Henan province[J].Chin J Health Lab Technol,2013,23(2):278-280.
[8]国家食品安全风险评估中心.国家食品污染物和有害因素风险监测工作手册(内部资料)[Z].2016.National Center for Food Safety Risk Assessment.National Manual for the Risk Monitoring of Food Contaminants and Harmful Factors(internal information)[Z].2016.
[9]国家食品安全风险评估中心.食源性疾病监测工作手册(内部资料)[Z].2016.National Center for Food Safety Risk Assessment.Manual for Monitoring Food-borne Diseases(internal information)[Z].2016.
[10]国家食品安全风险评估中心.国家食源性疾病分子溯源网络(TraNet China)使用手册(内部资料)[Z].2016.National Center for Food Safety Risk Assessment.Handbook of molecular traceability networks for foodborne diseases(internal information)[Z].2016.
[11]赵敏.细菌耐药现状及治疗[J].解放军医学杂志,2011,36(2):104-108.Zhao M.Drug resistance of bacteria-present situation and treatment[J].Med J Chin PLA,2011,36(2):104-108.
[12]杨晓洪,王娜,叶波平.畜禽养殖中的抗生素残留以及耐药菌和抗性基因研究进展[J].药物生物技术,2014,21(6):583-588.Yang XH,Wang N,Ye BP.Advances in the study of antibiotic residues and resistance bacteria and resistance genes in livestock and poultry breeding[J].Pharm Biotechnol,2014,21(6):583-588.
[13]李佩波,黄永茂.大肠埃希菌耐药及机制研究进展[J].西南军医,2011,13(1):95-97.Li PB,Huang YM.Research progress on resistance and mechanism of Escherichia coli[J].J Milit Surgeon Southwest China,2011,13(1):95-97.
[14]张东方,袁飞,陈颖,等.沙门菌分子分型方法研究进展[J].中国公共卫生,2012,28(1):117-120.Zhang DF,Yuan F,Chen Y,et al.Research progress on molecular typing of Salmonella[J].Chin J Public Health,2012,28(1):117-120.
[15]游兴勇,刘成伟,朱应飞,等.江西省食源性沙门菌血清分型及脉冲场凝胶电泳指纹图谱研究[J].中国食品卫生杂志,2014,26(6):528-532.You XY,Liu CW,Zhu YF,et al.Serotyping and PFGE type of Salmonella isolates from food in Jiangxi province[J].Chin J Food Hyg,2014,26(6):528-532.
[16]管峰,杨季芳.食源性致病菌溯源分型技术研究进展[J].浙江万里学院学报,2012,25(4):86-90.Guan F,Yang JF.Progress in research on anadromous differentiation of edible pathogens[J].J Zhejiang Wanli Univ,2012,25(4):86-90.
[17]崔志刚,王爱敏,王鸣柳,等.婴幼儿腹泻感染的非伤寒沙门菌分子分型和耐药情况研究[J].疾病监测,2014,29(6):428-431.Cui ZG,Wang AM,Wang ML,et al.Molecular subtyping and antibiotic resistance of non-typhi Salmonella isolated from infants[J].Dis Surveill,2014,29(6):428-431.
[18]谢晓红,赵宏伟,陈洪友,等.上海市奉贤区188株沙门菌分子分型研究[J].预防医学情报杂志,2018,34(11):1391-1395.Xie XH,Zhao HW,Chen HY,et al.Study on the molecular typing of 188Salmonella strains in fengxian district of Shanghai[J].J Prev Med Inf,2018,34(11):1391-1395.
[19]耿英芝,李飞,于淼,等,2016-2017年辽宁省沙门菌耐药菌谱与分子分型[J].中国微生态学杂志,2018,30(10):1142-1146.Geng YZ,Li F,Yu M,et al.Characteristics of drug resistance and molecular typing of Salmonella isolated in Liaoning,2016-2017[J].Chin J Microecol,2018,30(10):1142-1146.
[20]Long DL,Yan MY,Cui ZG,et al.Antimicrobial resistance research of non-typhoidal Salmonella enteric infections in four hospitals in Shenzhen,2012-2016[J].Chin J Prevent Med,2017,51(12):1118-1121.
[21]Andreoli G,Merla C,Valle CD,et al.Foodborne salmonellosis in Italy:Characterization of Salmonella enterica serovar typhimurium and monophasic variant 4,[5],12:i-isolated from salami and human patients[J].J Food Prot,2017,80(4):632-639.