猪皮酶解液分子量分布与除盐工艺考察
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摘要
目的:考察猪皮酶解液的分子量分布和除盐工艺,以筛选出最佳除盐方案。方法:以不同分子量物质的含量和除盐效果为指标,采用膜处理和电渗析除盐的方法对猪皮酶解液进行分子量分布测定和除盐工艺的考察。结果:通过对不同分子量物质的重量测定,得出猪皮肽在>3000 Da、1000～3000 Da和<1000 Da分子量下所占比例分别为21.36%、56.19%、4.72%,1000～3000 Da比例最高;通过电导率的变化、除盐率和肽回收率的考察,得出猪皮酶解液在电压31 V、流速15 L·h~(-1)、除盐时间2 h、药液浓度200 mg·mL~(-1)的条件下,效果最好,平均除盐率为93.58%,且肽回收率高达82.28%。结论:猪皮肽的分子量主要集中在1000～3000 Da,其除盐工艺合理可行,对后期实验进行和产品开发奠定了一定的实验基础。
Objective:To study the molecular weight distribution and desalting process of enzymolysis solution of spig skin,and to select the best desalting solution. Methods:The molecular weight distribution and desalting process of the enzymolysis solution of spig skin were studied by membrane treatment and electrodialysis desalting method with the contents of different molecular weight substances and desalting effect as indexes. Results:The results showed that the proportion of porcine peptides in the range of >3000 Da,1000-3000 Da and <1000 Da were 21.36%,56.19%,4.72% and 1000-3000 Da,respectively. The results showed that under the condition of voltage 31 V,flow rate 15 L·h~(-1),desalting time 2 h and concentration of liquid 200 mg·mL~(-1),with the best effect. The average salt removal rate was 93.58%,and the peptide recovery rate was 82.28%. Conclusion:The molecular weight of porcine peptide is mainly concentrated in 1000-3000 Da. The desalting process is reasonable and feasible. It has laid a certain practice foundation for the later experiment and product development.
Objective:To study the molecular weight distribution and desalting process of enzymolysis solution of spig skin,and to select the best desalting solution. Methods:The molecular weight distribution and desalting process of the enzymolysis solution of spig skin were studied by membrane treatment and electrodialysis desalting method with the contents of different molecular weight substances and desalting effect as indexes. Results:The results showed that the proportion of porcine peptides in the range of >3000 Da,1000-3000 Da and <1000 Da were 21.36%,56.19%,4.72% and 1000-3000 Da,respectively. The results showed that under the condition of voltage 31 V,flow rate 15 L·h~(-1),desalting time 2 h and concentration of liquid 200 mg·mL~(-1),with the best effect. The average salt removal rate was 93.58%,and the peptide recovery rate was 82.28%. Conclusion:The molecular weight of porcine peptide is mainly concentrated in 1000-3000 Da. The desalting process is reasonable and feasible. It has laid a certain practice foundation for the later experiment and product development.
引文
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[3]段镇基.中国猪皮制革的发展及现状[J].中国皮革,2008,37(3):56-57.
[4]王晨,李书平,李天铎,等.国内猪皮表层组织结构研究[J].皮革与化工,2002,19(2):1-2.
[5]余东华,徐志宏,魏振承.猪皮胶原蛋白的高效利用研究进展[J].食品工业科技,2011,32(2):359-361.
[6]周光敏.猪皮、猪蹄、猪血做为高蛋白食品在美容保健领域的探索研究[J].资源节约和综合利用,1997(2):48-54.
[7]陆胜麟.重视猪皮资源的发开利用[J].食品研究与开发,1995(2):5-8.
[8]李开雄,赵志远,刘霞.猪皮中胶原蛋白的提取及其应用[J].肉类研究,1996,4:43-46,48.
[9]王学川,任龙芳,强涛涛,等.胶原蛋白的研究进展及其在化妆品中的应用[J].日用化学工业,2005,6:388-392.
[10]王昱琳.胶原蛋白在化妆品中的应用研究进展[J].明胶科学与技术,2012,1:8-12.
[11]将挺大.胶原与胶原蛋白[M].北京:化学工业出版社,2006:33.
[12]阮仁全.多肽促人表皮生长因子透皮输运机理及其应用研究[D].合肥:中国科学技术大学,2015.
[13]李广,梁艳玲,韦宏.电渗析技术的发展及应用[J].化工技术与开发,2008,7:28-30.
[14]赵胜年.酶解鲜猪皮提取水解胶原蛋白的研究[J].食品工业科技,1998,5:18-19.
[15]姜忠义,吴洪.超滤膜分离技术在中药制剂生产中的应用[J].化学工业与工程,2003,1:39-44.
[16]胡二坤,郭兴凤,谭凤艳,等.猪皮中胶原蛋白的提取[J].河南工业大学学报(自然科学版),2006,1:50-53.
[17]李学梅,刘茉娥.发酵液中乳酸的电渗析法分离[J].高校化学工程学报,1998,3:231.