茶树CSD1基因及其启动子克隆与低温胁迫下的表达
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摘要
低温寒害严重影响茶树的生长发育状况及成品茶品质,Copper/zinc-superoxide dismutase(CSD)基因在植物抗胁迫响应中起着关键作用.为了解茶树CSD基因(CsCSD)响应低温胁迫的作用机制,以铁观音茶树叶片为供试材料,采用同源克隆结合RACE方法及染色体步移法,获得茶树CSD1基因的cDNA序列(GenBank登录号:KR078346)、gDNA序列及其启动子序列,并对其进行生物信息学分析,同时对低温胁迫处理下CsCSD1基因的表达模式也进行分析.结果显示,茶树CsCSD1基因cDNA全长860 bp,完整的开放阅读框(ORF)长度为459 bp,共编码152个氨基酸;gDNA基因结构分析发现CsCSD1基因由6个外显子和5个内含子构成;CpG岛预测发现启动子区域内存在1个CpG岛,CpG长度为219bp,GC含量为50.3%;CsCSD1启动子上还存在大量的顺式作用元件,包括光响应元件、激素响应元件、胁迫响应元件和其他响应元件;在低温胁迫下CsCSD1基因的表达模式显示,在低温胁迫前期,CsCSD1基因表达上调;随后CsCSD1基因的表达量不断下降.本研究表明,CsCSD1基因能够响应低温胁迫并在胁迫的不同时期发挥不同的作用,推测与低温胁迫密切相关.
The growth and quality of tea are severely affected by low temperature. Copper/zinc-superoxide dismutase(CSD) genes play crucial roles in the response of plants to stress. The objective of this study was to explore the response mechanism of CSD gene in tea(CsCSD) under low temperature stress. The leaves of Camellia sinensis "Tieguanyin" were used as raw material. The full-length cDNA(GenBank accession number: KR078346), gDNA, and promoter sequence of CsCSD1 were cloned using homology-based cloning, combined with RACE and chromosome walking. Moreover, the bioinformatic characteristics and expression patterns of CsCSD1 under low temperature stress were investigated. The results showed that the full-length cDNA of CsCSD1 was 860 bp long, containing a 459 bp open reading frame(ORF) which encodes a protein of 152 amino acids. The gDNA structural analysis found that the CsCSD1 gene was composed of 6 exons and 5 introns. In addition, the CpG island prediction found that there was only a CpG island in the promoter region. The length of the CpG island was 219 bp and the GC content was 50.3%. The CsCSD1 promoter also contained a large number of cis-acting elements, including light response elements, hormone response elements, stress response elements, and other response elements. Expression analysis revealed that the expression of CsCSD1 was up-regulated at the early stage of low temperature stress, and then the expression decreased continuously. This study showed that the CsCSD1 gene can respond to low temperature stress and play different roles in different periods of low temperature stress.
The growth and quality of tea are severely affected by low temperature. Copper/zinc-superoxide dismutase(CSD) genes play crucial roles in the response of plants to stress. The objective of this study was to explore the response mechanism of CSD gene in tea(CsCSD) under low temperature stress. The leaves of Camellia sinensis "Tieguanyin" were used as raw material. The full-length cDNA(GenBank accession number: KR078346), gDNA, and promoter sequence of CsCSD1 were cloned using homology-based cloning, combined with RACE and chromosome walking. Moreover, the bioinformatic characteristics and expression patterns of CsCSD1 under low temperature stress were investigated. The results showed that the full-length cDNA of CsCSD1 was 860 bp long, containing a 459 bp open reading frame(ORF) which encodes a protein of 152 amino acids. The gDNA structural analysis found that the CsCSD1 gene was composed of 6 exons and 5 introns. In addition, the CpG island prediction found that there was only a CpG island in the promoter region. The length of the CpG island was 219 bp and the GC content was 50.3%. The CsCSD1 promoter also contained a large number of cis-acting elements, including light response elements, hormone response elements, stress response elements, and other response elements. Expression analysis revealed that the expression of CsCSD1 was up-regulated at the early stage of low temperature stress, and then the expression decreased continuously. This study showed that the CsCSD1 gene can respond to low temperature stress and play different roles in different periods of low temperature stress.
引文
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3李应发,张怀清,刘中军,何天军,何永强,白涛,董晓勇,罗长城.宁强县2015/2016茶园冻寒害调查报告[J].陕西农业科学,2016,62(8):69-70[Li YF,Zhang HQ,Liu ZJ,He TJ,He YQ,Bai T,Dong XY,Luo CC.Ningqiang county tea garden 2015/2016 freezing survey report of chilling injury[J].Shanxi J Agric Sci,2016,62(8):69-70]
4薛徐燕,沈久聪.强寒潮后屏南县茶园冻害情况调查[J].中国茶叶,2017,39(5):40-44[Xue XY,Shen JC.Investigation on the freezing damage to tea garden in Pingnan County after the strong cold wave[J].Chin Tea,2017,39(5):40-44]
5 Abreu IA,Cabelli DE.Superoxide dismutases-a review of the metalassociated mechanistic variations[J].BBA-Proteins Proteom,2010,1804(2):263-274
6 Alscher RG,Erturk N,Heath LS.Role of superoxide dismutases(SODs)in controlling oxidative stress in plants[J].J Exp Bot,2002,53(372):1331-1341
7 Kliebenstein DJ,Last RL.Superoxide dismutase in Arabidopsis:an eclectic enzyme family with disparate regulation and protein localization[J].Plant Physiol,1998,118(2):637-650
8 Zhang DY,Yang HL,Li XS,Li HY,Wang YC.Overexpression of Tamarix albiflonum TaMnSOD increases drought tolerance in transgenic cotton[J].Mol Breeding,2014,34(1):1-11
9 Faize M,Burgos L,Faize L,Piqueras A,Nicolas E,Barba-Espin G,Clemente-Moreno MJ,Alcobendas R,Artlip T,Hernandez JA.Involvement of cytosolic ascorbate peroxidase and Cu/Zn-superoxide dismutase for improved tolerance against drought stress[J].J Exp Bot,2011,62(8):2599-2613
10王伟青.番茄叶绿体抗氧化酶基因的克隆与遗传转化的研究[D].泰安:山东农业大学,2003[Wang WQ.Gene cloning and transformation of antioxidant enzymes in the chloroplasts of tomato[D].Taian:Shandong Agricultural University,2003]
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