益生菌结合甲硝唑用于家兔肠道疾病的改善作用
|
摘要
为研究益生菌结合甲硝唑对家兔肠道疾病的改善作用,并为家兔的肠道疾病治疗提供用药剂量依据;40只受A型产气荚膜梭菌感染的家兔作为主要研究对象,分为甲硝唑组10只,益生菌组10只,益生菌结合甲硝唑大剂量(结合A)组10只,益生菌结合甲硝唑小剂量(结合B)组10只,另选取10只健康家兔作为对照组,对比5组家兔的存活率、兔十二指肠黏膜厚度、绒毛长度、肠道菌群情况、空肠形态结构变化;结果显示,益生菌组病死率显著高于其他3组,且存在显著差异(P<0.01);甲硝唑组与甲硝唑A、B组比较无差异;兔十二指肠组织形态学对比,甲硝唑组的黏膜厚度,与对照组比较显著降低(P<0.05)。其他3组与对照组比较无显著差异(P>0.05);兔空肠组织形态学对比,空肠黏膜厚度,甲硝唑组与益生菌组均显著高于对照组,且益生菌组的绒毛长度也显著增高,甲硝唑A组显著高于对照组空肠绒毛长度(P<0.05);与对照组比较,甲硝唑组及甲硝唑A组大肠杆菌总数均显著低于对照组(P<0.01);甲硝唑组乳酸菌总量显著低于对照组(P<0.01);益生菌组产气荚膜梭菌量显著高于对照组(P<0.01);甲硝唑B组显著低于对照组产气荚膜梭菌总量(P<0.01);对于家兔的肠道疾病,尤其是产气荚膜梭菌感染,单纯采用益生菌的病死率高,单纯采用甲硝唑治疗容易造成肠道菌群失调,建议采用能够修复肠道黏膜并不会增加家兔病死率的益生菌结合小剂量甲硝唑治疗。
To study the effect of probiotics combined with metronidazole on the improvement of intestinal diseases in rabbits and to provide dosage basis for the treatment of intestinal diseases in rabbits.Forty rabbits infected with Clostridium perfringens type A were divided into metronidazole group(n=10),probiotics group(n=10)and probiotics combined with metronidazole group(n=10).There were 10 probiotics combined with metronidazole(combined with B)and 10 healthy rabbits as control group.The survival rate,duodenal mucosal thickness,villi length,intestinal flora and jejunum morphology of the five groups were compared.Results showed that:the mortality of probiotic group was significantly higher than that of the other three groups(P<0.01).There was no difference between the metronidazole group and metronidazole A,the B group,and the mucosa thickness of the rabbit duodenum and the metronidazole group was significantly lower than that of the control group(P<0.05).There was no significant difference between the other three groups and the control group(P>0.05).The histomorphological comparison of jejunum,the thickness of jejunum mucosa,the metronidazole group and probiotic group were significantly higher than the control group,and the length of the probiotic group increased significantly,and the metronidazole A group was significantly higher than that of the control group(P<0.05).The total number of Escherichia coli in metronidazole group and metronidazole A group was significantly lower than that in control group(P<0.01),the total amount of lactic acid bacteria in metronidazole group was significantly lower than that in control group(P<0.01),the amount of Clostridium perfringens in probiotics group was significantly higher than that in control group(P<0.01).The total amount of Clostridium perfringens in metronidazole group B was significantly lower than that in control group(P<0.01).Conclusion showed that for intestinal diseases of rabbits,especially Clostridium perfringens infection,the mortality of probiotics alone is high,and metronidazole alone can easily cause intestinal flora disorders.Probiotics combined with low dose metronidazole can repair intestinal mucosa and do not increase the mortality of rabbits.
To study the effect of probiotics combined with metronidazole on the improvement of intestinal diseases in rabbits and to provide dosage basis for the treatment of intestinal diseases in rabbits.Forty rabbits infected with Clostridium perfringens type A were divided into metronidazole group(n=10),probiotics group(n=10)and probiotics combined with metronidazole group(n=10).There were 10 probiotics combined with metronidazole(combined with B)and 10 healthy rabbits as control group.The survival rate,duodenal mucosal thickness,villi length,intestinal flora and jejunum morphology of the five groups were compared.Results showed that:the mortality of probiotic group was significantly higher than that of the other three groups(P<0.01).There was no difference between the metronidazole group and metronidazole A,the B group,and the mucosa thickness of the rabbit duodenum and the metronidazole group was significantly lower than that of the control group(P<0.05).There was no significant difference between the other three groups and the control group(P>0.05).The histomorphological comparison of jejunum,the thickness of jejunum mucosa,the metronidazole group and probiotic group were significantly higher than the control group,and the length of the probiotic group increased significantly,and the metronidazole A group was significantly higher than that of the control group(P<0.05).The total number of Escherichia coli in metronidazole group and metronidazole A group was significantly lower than that in control group(P<0.01),the total amount of lactic acid bacteria in metronidazole group was significantly lower than that in control group(P<0.01),the amount of Clostridium perfringens in probiotics group was significantly higher than that in control group(P<0.01).The total amount of Clostridium perfringens in metronidazole group B was significantly lower than that in control group(P<0.01).Conclusion showed that for intestinal diseases of rabbits,especially Clostridium perfringens infection,the mortality of probiotics alone is high,and metronidazole alone can easily cause intestinal flora disorders.Probiotics combined with low dose metronidazole can repair intestinal mucosa and do not increase the mortality of rabbits.
引文
[1]杨睿,黎春秀,付利芝,等.不同日龄家兔肠道微生物群落结构[J].中国兽医学报,2017,37(9):1693-1698.
[2]崔一喆,王秋菊,苏景,等.复合益生菌制剂对离乳梅花鹿生长性能、粪便菌群及血液生化指标的影响[J].中国兽医学报,2017,37(1):129-133.
[3]艾红英,彭富强,黄娟,等.益生菌益生作用的新机制—作为肠道抗菌肽分泌的促进剂[J].中国兽医学报,2016,36(6):1076-1080.
[4]田浪,何彦侠,侯月娥,等.复合益生菌制剂对黄羽肉鸡生产性能、抗氧化指标及免疫功能的影响[J].中国兽医学报,2017,37(8):1540-1544.
[5]李晶,沈雪梅,刘丽慧,等.獭兔肠黏膜乳杆菌的分离鉴定及其体外益生特性研究[J].西北农林科技大学学报(自然科学版),2017,45(4):24-30.
[6]孙佳易,杨桂芹,郭东新,等.饲粮纤维源及粗纤维水平对肉兔胃肠道发育及十二指肠组织形态的影响[J].沈阳农业大学学报,2016,47(3):291-298.
[7]MIRA E,BENAZZO M,GALIOTO P,et al.Presence of phospholipidic lamellar bodies on the mucosa of rabbit eustachian tube[J].ORL,2016,50(4):251-256.
[8]LU Y,PUTRA S D,LIU S Q.A novel non-dairy beverage from durian pulp fermented with selected probiotics and yeast[J].Int J Food Microbiol,2018,265:1-8.
[9]ALLAIN D,HDE R,THEBAULT R G,et al.The inheritance of wool quantity and live weight in the French Angora rabbit[J].Animal Sci,2016,68(3):441-447.
[10] LIU Y,ZHAO J,LIAO D,et al.Intestinal mechanomorphological remodeling induced by long-term lowfiber diet in rabbits[J].Ann Biomed Eng,2017:1-12.
[11] KOBYLIAK N,CONTE C,CAMMAROTA G,et al.Probiotics in prevention and treatment of obesity:a critical view[J].Diabetes Nutr Metab,2016,13(1):1-13.
[12]MOHAMMADI A A,JAZAYERI S,KHOSRAVIDARANI K,et al.The effects of probiotics on mental health and hypothalamic-pituitary-adrenal axis:A randomized,double-blind,placebo-controlled trial in petrochemical workers[J].Nutr Neurosci,2016,19(9):387-395.
[13] GIBSON G R,HUTKINS R,SANDERS M E,et al.Expert consensus document:The International Scientific Association for Probiotics and Prebiotics(ISAPP)consensus statement on the definition and scope of prebiotics[J].Nat Rev Gastro Hepat,2017,14(8):491.
[14] KADRY M O,MEGEED R A.Probiotics as a Complementary Therapy in the Model of Cadmium Chloride Toxicity:Crosstalk ofβ-Catenin,BDNF,and StAR Signaling Pathways[J].Biol Trace Elem Res,2018(1/2):1-10.
[15] ABDULKADIR B,NELSON A,SKEATH T,et al.Routine use of probiotics in preterm infants:longitudinal impact on the microbiome and metabolome[J].Neonatology,2016,109(4):239-247.
[16] OH B,KIM B S,Kim J W,et al.The Effect of probiotics on gut microbiota during the helicobacter pylori eradication:randomized controlled trial[J].Helicobacter,2016,21(3):165-174.
[17] JEONG Y D,KO H S,HOSSEINDOUST A,et al.82Effects of dietary lactose levels and supplementation of probiotics on growth performance in weanling pigs[J].J Animal Sci,2018,96(suppl 2):42-43.
[18] QUIROZGUZMN E,VZQUEZJUREZ R,LUNAGONZLEZ A,et al.Administration of probiotics improves the brine shrimp production and prevents detrimental effects of pathogenic vibrio species[J].Marine Biotechnol,2018,20(4):1-8.
[19] KHAN A Z,KUMBHAR S,LIU Y,et al.Dietary supplementation of selenium-enriched probiotics enhances meat quality of broiler chickens(gallus gallus domesticus)raised under high ambient temperature[J].Biol Trace Elem Res,2018,182(2):328-338.
[2]崔一喆,王秋菊,苏景,等.复合益生菌制剂对离乳梅花鹿生长性能、粪便菌群及血液生化指标的影响[J].中国兽医学报,2017,37(1):129-133.
[3]艾红英,彭富强,黄娟,等.益生菌益生作用的新机制—作为肠道抗菌肽分泌的促进剂[J].中国兽医学报,2016,36(6):1076-1080.
[4]田浪,何彦侠,侯月娥,等.复合益生菌制剂对黄羽肉鸡生产性能、抗氧化指标及免疫功能的影响[J].中国兽医学报,2017,37(8):1540-1544.
[5]李晶,沈雪梅,刘丽慧,等.獭兔肠黏膜乳杆菌的分离鉴定及其体外益生特性研究[J].西北农林科技大学学报(自然科学版),2017,45(4):24-30.
[6]孙佳易,杨桂芹,郭东新,等.饲粮纤维源及粗纤维水平对肉兔胃肠道发育及十二指肠组织形态的影响[J].沈阳农业大学学报,2016,47(3):291-298.
[7]MIRA E,BENAZZO M,GALIOTO P,et al.Presence of phospholipidic lamellar bodies on the mucosa of rabbit eustachian tube[J].ORL,2016,50(4):251-256.
[8]LU Y,PUTRA S D,LIU S Q.A novel non-dairy beverage from durian pulp fermented with selected probiotics and yeast[J].Int J Food Microbiol,2018,265:1-8.
[9]ALLAIN D,HDE R,THEBAULT R G,et al.The inheritance of wool quantity and live weight in the French Angora rabbit[J].Animal Sci,2016,68(3):441-447.
[10] LIU Y,ZHAO J,LIAO D,et al.Intestinal mechanomorphological remodeling induced by long-term lowfiber diet in rabbits[J].Ann Biomed Eng,2017:1-12.
[11] KOBYLIAK N,CONTE C,CAMMAROTA G,et al.Probiotics in prevention and treatment of obesity:a critical view[J].Diabetes Nutr Metab,2016,13(1):1-13.
[12]MOHAMMADI A A,JAZAYERI S,KHOSRAVIDARANI K,et al.The effects of probiotics on mental health and hypothalamic-pituitary-adrenal axis:A randomized,double-blind,placebo-controlled trial in petrochemical workers[J].Nutr Neurosci,2016,19(9):387-395.
[13] GIBSON G R,HUTKINS R,SANDERS M E,et al.Expert consensus document:The International Scientific Association for Probiotics and Prebiotics(ISAPP)consensus statement on the definition and scope of prebiotics[J].Nat Rev Gastro Hepat,2017,14(8):491.
[14] KADRY M O,MEGEED R A.Probiotics as a Complementary Therapy in the Model of Cadmium Chloride Toxicity:Crosstalk ofβ-Catenin,BDNF,and StAR Signaling Pathways[J].Biol Trace Elem Res,2018(1/2):1-10.
[15] ABDULKADIR B,NELSON A,SKEATH T,et al.Routine use of probiotics in preterm infants:longitudinal impact on the microbiome and metabolome[J].Neonatology,2016,109(4):239-247.
[16] OH B,KIM B S,Kim J W,et al.The Effect of probiotics on gut microbiota during the helicobacter pylori eradication:randomized controlled trial[J].Helicobacter,2016,21(3):165-174.
[17] JEONG Y D,KO H S,HOSSEINDOUST A,et al.82Effects of dietary lactose levels and supplementation of probiotics on growth performance in weanling pigs[J].J Animal Sci,2018,96(suppl 2):42-43.
[18] QUIROZGUZMN E,VZQUEZJUREZ R,LUNAGONZLEZ A,et al.Administration of probiotics improves the brine shrimp production and prevents detrimental effects of pathogenic vibrio species[J].Marine Biotechnol,2018,20(4):1-8.
[19] KHAN A Z,KUMBHAR S,LIU Y,et al.Dietary supplementation of selenium-enriched probiotics enhances meat quality of broiler chickens(gallus gallus domesticus)raised under high ambient temperature[J].Biol Trace Elem Res,2018,182(2):328-338.