3株猕猴桃细菌性溃疡病致病菌的药剂室内毒力比较
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摘要
为了筛选猕猴桃细菌性溃疡病的有效防治药剂,采用抑菌圈法比较28种杀菌剂对不同猕猴桃细菌性溃疡病3株致病菌(PSA_1、PSA_2、PSA_3)的室内毒力,并测定药剂间复配对致病菌的联合毒力。结果表明,28种杀菌剂中20%溴硝醇、20%叶枯唑、40%百菌清3种杀菌剂对PSA_1的毒力效果较强,其EC_(50)值分别为32.56、43.12、44.06 mg/L,均小于对照药剂72%硫酸链霉素(对PSA_1的毒力效果EC_(50)值分别为44.56、54.56、77.82 mg/L)的毒力效果;而40%百菌清对PSA_2和PSA_3表现出较好的毒力作用,其EC_(50)值分别为53.21、54.45 mg/L,优于对照药剂硫酸链霉素。不同比例复配药剂的室内毒力筛选发现,20%溴硝醇+20%叶枯唑(质量比为3∶1)和20%溴硝醇+40%百菌清(质量比为3∶1)2种复配剂对PSA_1表现出较强的毒力,且为增效作用,均可作为田间猕猴桃细菌性溃疡病的有效防控药剂。
引文
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[4]Dreo T,Pirc M,Ravnikar M,et al.First report of Pseudomonas syringae pv.actinidiae,the causal agent of bacterial canker of kiwifruit in Slovenia[J].Plant Disease,2011,95(95):1311-1311.
[5]Everett K R,Taylor R K,Romberg M K,et al.First report of Pseudomonas syringae pv.actinidiae causing kiwifruit bacterial canker in New Zealand[J].Australasian Plant Disease Notes,2011,6(1):67-71.
[6]Abelleira A,López M M,Peňalver J,et al.First report of bacterial canker of kiwifruit caused by Pseudomonas syringae pv.actinidiae in Spain[J].Plant Disease,2011,95(12):1583.
[7]Liu Y,Li S J,Zhu T H,et al.Specific DNA markers for detection of bacterial canker of kiwifruit in Sichuan,China[J].African Journal of Microbiology Research,2012,6(49):7512-7519.
[8]Bastas K K,Karakaya A.First report of bacterial canker of kiwifruit caused by Pseudomonas syringae pv.actinidiae in Turkey[J].Plant Disease,2012,96(3):452.
[9]高小宁,赵志博,黄其玲,等.猕猴桃细菌性溃疡病研究进展[J].果树学报,2012,29(2):262-268.
[10]吴乾守.两种乙蒜素复配剂防治水稻稻瘟病的田间应用研究[J].福建稻麦科技,2007,25(2):17-18.
[11]高蓬明,王瑞,马立志.猕猴桃溃疡病防治药剂的室内毒力及田间防治效果[J].贵州农业科学,2013,41(4):85-88.
[12]王瑞,马立志,高蓬明,等.乙蒜素与溴硝醇、代森锰锌2种农药复配对丁香假单胞杆菌的联合毒力[J].江苏农业科学,2014,42(3):71-73.
[13]方中达.植病研究方法[M].3版.北京:中国农业出版社,1998.
[14]Mazzaglia A,Studholme D J,Taratufolo M C,et al.Pseudomonas syringae pv.actinidiae (PSA) isolates from recent bacterial canker of kiwifruit outbreaks belong to the same genetic lineage[J].PLoS One,2012,7(5):e36518.
[15]孙广宇,宗兆锋.植物病理学实验技术[M].北京:中国农业出版社,2002.
[16]Sun Y P,Johnson E R.Analysis of joint action of insecticidesagainst house flies[J].Journal of Economic Entomology,1960,53(5):887-892.
[17]张瑞亭.农药的混用与混剂[M].北京:化学工业出版社,1987.
[18]龙云川,彭熙,李苇洁,等.贵州猕猴桃溃疡病病菌分离鉴定及其培养特性[J].江苏农业科学,2017,45(20):114-117.蒙成,黄艳花.玉米小斑病抗病鉴定接种培养基的产孢技术[J].江苏农业科学,2019,47(11):144-147.