心脏原位垫线结扎法建立大鼠心肌缺血再灌注模型
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摘要
目的:用心脏原位垫线结扎法制备大鼠心肌缺血再灌注模型,简化实验操作,提高造模成功率。方法:健康SD大鼠随机分为假手术组、缺血组和缺血再灌注共3组,从心电图、心肌梗死面积、死亡率等方面对该模型进行评估。结果:用心脏原位垫线结扎法制备的心肌缺血再灌注模型较心肌缺血组可明显缩小心肌梗死面积,降低大鼠死亡率。结论:该建模方法较为简单易行,可靠性和成功率均有保障。
Objective: To establish myocardial ischemia-reperfusion rat models by in situ cardiac cushion ligation,simplify the experimental operation and improve the success rate of model building. Methods: Healthy SD rats were randomly divided into the sham operation group,the ischemic group and the ischemia reperfusion group,with the models evaluated from the perspectives of electrocardiogram,success rates and mortality rates. Results: The area of myocardial infarction of the myocardial ischemia-reperfusion models established by in situ cardiac cushion ligation was significantly reduced compared with that of the myocardial ischemia group,lowering the mortality rate of rats. Conclusion: This method is simpler and easier,with the reliability and the success rate guaranteed.
Objective: To establish myocardial ischemia-reperfusion rat models by in situ cardiac cushion ligation,simplify the experimental operation and improve the success rate of model building. Methods: Healthy SD rats were randomly divided into the sham operation group,the ischemic group and the ischemia reperfusion group,with the models evaluated from the perspectives of electrocardiogram,success rates and mortality rates. Results: The area of myocardial infarction of the myocardial ischemia-reperfusion models established by in situ cardiac cushion ligation was significantly reduced compared with that of the myocardial ischemia group,lowering the mortality rate of rats. Conclusion: This method is simpler and easier,with the reliability and the success rate guaranteed.
引文
[1] Ferez Santander SM,Marquez MF,Pena Duque MA,et al.Myocardial reperfusion injury[J]. Rev Esp Cardiol,2004,57(1):9-21.
[2]刘付平,姚宏伟,李俊.大鼠心肌缺血再灌注损伤模型的改进[J].安徽医科大学学报,2003,38(3):234-236.
[3]张润峰,王继生.建立大鼠心肌梗死损伤的若干问题探讨[J].山西医科大学学报,2004,35(1):13-15.
[4]王淑霞,协小莉,王吉文,等.大鼠心肌缺血/再灌注损伤模型的改进与评价[J].解剖科学进展,2000,6(1):371-373.
[5]侯梦梦,邢冬梅,刘璇,等.大鼠心肌缺血再灌注损伤模型建立的关键性问题[J].天津中医药大学学报,2014,33(6):323-326.
[6]齐志敏,王倩,张莹.大鼠心肌缺血再灌注损伤实验模型研究[J].中国临床康复,2004,30(8):6620-6621.
[7]李亚辉,何建国,乔木,等.直视下行大鼠气管插管的改良方法[J].中国动物学实验杂志,2000,10(2):123-125.
[8]相磊,罗斌,伍新尧,等.大鼠急性心肌梗死心肌ER表达的变化规律[J].中国的法医学杂志,2005,20(5):83.
[9]徐淑云,卞如濂,陈如修.药理实验方法学[M]. 2版.北京:人民卫生出版社,1991:938.
[2]刘付平,姚宏伟,李俊.大鼠心肌缺血再灌注损伤模型的改进[J].安徽医科大学学报,2003,38(3):234-236.
[3]张润峰,王继生.建立大鼠心肌梗死损伤的若干问题探讨[J].山西医科大学学报,2004,35(1):13-15.
[4]王淑霞,协小莉,王吉文,等.大鼠心肌缺血/再灌注损伤模型的改进与评价[J].解剖科学进展,2000,6(1):371-373.
[5]侯梦梦,邢冬梅,刘璇,等.大鼠心肌缺血再灌注损伤模型建立的关键性问题[J].天津中医药大学学报,2014,33(6):323-326.
[6]齐志敏,王倩,张莹.大鼠心肌缺血再灌注损伤实验模型研究[J].中国临床康复,2004,30(8):6620-6621.
[7]李亚辉,何建国,乔木,等.直视下行大鼠气管插管的改良方法[J].中国动物学实验杂志,2000,10(2):123-125.
[8]相磊,罗斌,伍新尧,等.大鼠急性心肌梗死心肌ER表达的变化规律[J].中国的法医学杂志,2005,20(5):83.
[9]徐淑云,卞如濂,陈如修.药理实验方法学[M]. 2版.北京:人民卫生出版社,1991:938.