生长年限对窝儿七药材中主要成分的影响
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摘要
目的通过检测窝儿七中槲皮素、4′-去甲基鬼臼毒素、鬼臼毒素、山柰酚4个活性成分的含量来观察不同年限窝儿七中主要成分变化。方法采用高效液相色谱法对窝儿七中槲皮素、4′-去甲基鬼臼毒素、鬼臼毒素、山柰酚四种化学成分的含量进行测定。结果 1年至8年生窝儿七药材中,鬼臼毒素在不同年限窝儿七药材中含量均最高,六年生的药材中槲皮素、4′-去甲基鬼臼毒素、鬼臼毒素、山柰酚四种化学成分的综合含量最高。结论通过实验研究,六年生的窝儿七主要成分含高较高,质量最佳,为药材的栽培与应用以及质量规范的制订,提供理论和试验依据。
Objective To observe changes of the main components at different ages in umbrellaleaf rhizome and root by measuring the contents of four active ingredients of quercetin, 4'-demethylpodophyllotoxin, podophyllotoxin and kaempferol. Methods Determination of four chemical constituents: quercetin, 4'-demethylpodophyllotoxin, podophyllotoxin and kaempferol was carried out by using High Performance Liquid Chromatography(HPLC).Results Podophyllotoxin was the most abundant in the 1-to-8-year-old umbrellaleaf rhizome and root. Quercetin, 4'-demethylpodophyllotoxin, podophyllotoxin and kaempferol were the most abundant in the 6-year-old umbrellaleaf rhizome and root.Conclusion Through experimental research, the six-year-old umbrellaleaf rhizome and root have the higher major contents and best quality, providing theoretical and experimental basis for the cultivation and application of medicinal materials and the formulation of quality specifications.
Objective To observe changes of the main components at different ages in umbrellaleaf rhizome and root by measuring the contents of four active ingredients of quercetin, 4'-demethylpodophyllotoxin, podophyllotoxin and kaempferol. Methods Determination of four chemical constituents: quercetin, 4'-demethylpodophyllotoxin, podophyllotoxin and kaempferol was carried out by using High Performance Liquid Chromatography(HPLC).Results Podophyllotoxin was the most abundant in the 1-to-8-year-old umbrellaleaf rhizome and root. Quercetin, 4'-demethylpodophyllotoxin, podophyllotoxin and kaempferol were the most abundant in the 6-year-old umbrellaleaf rhizome and root.Conclusion Through experimental research, the six-year-old umbrellaleaf rhizome and root have the higher major contents and best quality, providing theoretical and experimental basis for the cultivation and application of medicinal materials and the formulation of quality specifications.
引文
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[9] 蔡云朗,高永星,任慕兰,等.山奈酚对子宫肌瘤体外抑制作用的实验研究[J].实用妇产科杂志,2014,30(5):350-353.
[10] 周瑶,杜标炎,谭宇蕙,等.山奈酚对大鼠肝癌细胞CBRH7919的增殖抑制及诱导凋亡作用[J].广州中医药大学学报,2010,27(3):250-253+317.
[11] 杜标炎,周瑶,谭宇蕙,等.山奈酚对人胃癌MGC-803细胞的生长抑制及诱导凋亡作用[J].肿瘤,2010,30(6):477-480.
[12] 赖菁华,宋小妹.窝儿七中3种木脂素的定性定量研究[J].中医药导报,2015,21(24):35-36,39.
[13] 马辰,杨峻山,罗淑荣.山荷叶中木脂素成分的研究[J].药学学报,1993,28(9):690-694.
[14 ]白吉庆,王小平,孙涛,等.不同产地窝儿七中黄酮和木质素的含量测定[J].中医药导报,2012,18(7):80-82.
[15 ]王小平,黎丹,龙凯花,等.一测多评法测定窝儿七中3个活性成分含量[J].药物分析杂志,2014,34(8):1426-1430.
[2]宝鸡卫生局.太白上本草志[M].西安:西安科技出版社,1993:442.
[3] 张丽,王菲,许欢,等.窝儿七的研究进展[J].现代中医药,2017,37(1):89-92.
[4] 赖菁华,宋小妹.窝儿七化学成分研究[J].中南药学,2017,15(3):308-311.
[5]李真,范冬冬,张东东,等.窝儿七化学成分研究[J].西北药学杂志,2015,30(6):666-668.
[6]杨秀芳,马养民,郑敬海,等.窝儿七中化学成分活性的研究[J].陕西科技大学学报(自然科学版),2013,31(1):86-89.
[7]郑敬海.窝儿七化学成分的研究[D].西安陕西科技大学,2012.
[8] 马养民,郑敬海,刘建军,等.太白七药窝儿七化学成分的研究[J].安徽农业科学,2011,39(32):19781-19782.
[9] 蔡云朗,高永星,任慕兰,等.山奈酚对子宫肌瘤体外抑制作用的实验研究[J].实用妇产科杂志,2014,30(5):350-353.
[10] 周瑶,杜标炎,谭宇蕙,等.山奈酚对大鼠肝癌细胞CBRH7919的增殖抑制及诱导凋亡作用[J].广州中医药大学学报,2010,27(3):250-253+317.
[11] 杜标炎,周瑶,谭宇蕙,等.山奈酚对人胃癌MGC-803细胞的生长抑制及诱导凋亡作用[J].肿瘤,2010,30(6):477-480.
[12] 赖菁华,宋小妹.窝儿七中3种木脂素的定性定量研究[J].中医药导报,2015,21(24):35-36,39.
[13] 马辰,杨峻山,罗淑荣.山荷叶中木脂素成分的研究[J].药学学报,1993,28(9):690-694.
[14 ]白吉庆,王小平,孙涛,等.不同产地窝儿七中黄酮和木质素的含量测定[J].中医药导报,2012,18(7):80-82.
[15 ]王小平,黎丹,龙凯花,等.一测多评法测定窝儿七中3个活性成分含量[J].药物分析杂志,2014,34(8):1426-1430.