复元活血汤对大鼠股骨骨折后血清生长激素及骨痂GH受体表达水平影响
|
摘要
目的:探讨复元活血汤对大鼠股骨骨折后血清生长激素(GH)及骨痂GH受体表达水平影响。方法:选取由本院实验动物中心提供清洁级雄性SD大鼠30只,大鼠腹腔内注射10%水合氯醛麻醉,股骨中断使用低速牙科钻将股骨切断,使用克氏针由髓腔内逆行固定造模,观察组大鼠给予复元活血汤灌胃,对照组则使用等剂量生理盐水灌胃,每天一次,共进行28天,在给药后10天、20天、28天分别选取5只大鼠,麻醉处死,全自动血流变测试仪器检测给药10、20、28天后不同剪切速率时大鼠血浆粘度与全血粘度,比色法检测血清磷(P)、钙(Ca)、ALP含量,ELISA检测血清GH含量,免疫组法检测骨痂组织内GHR表达状况。结果:给药后20、28天观察组大鼠全血30s-1、5s-1及1s-1切变率均低于对照组,差异有统计学意义(P<0. 05);给药后20、28天观察组大鼠血清GH、P、Ca及ALP水平均高于对照组,差异有统计学意义(P<0. 05);给药20、28天后观察组大鼠GHR表达高于对照组,血浆粘度低于对照组,差异均有统计学意义(P<0. 05)。结论:复元活血汤可使股骨骨折大鼠血清GH活性增强,降解速度减缓,骨痂组织内GHR表达量升高,进而发挥加速骨折愈合作用。
Objective: To investigate the effect of Fuyuan Huoxue Decoction on serum GH and callus GH receptor expression in femoral fractures of rats. Methods: Thirty male SD rats were randomly divided into experimental group and controlled group.Rats were anesthetized by intraperitoneal injection of 10% chloral hydrate,the femur was cut off with low-speed dental drill,and the Kirschner wire was retrograde fxed modeling,the observation group rats were given Fuyuan Huoxue Decoction gavage,the controlled group was treated with an equal dose of saline,once a day for a total of 28 days,10days after administration,20 days,28days were selected 5rats were anesthetized and sacrificed. The automatic blood rheology testing instrument was used to detect the plasma viscosity and whole blood viscosity of rats at different shear rates 10,20 and 28days after the administration. The contents of serum phosphorus(P),calcium(Ca),ALP content,serum GH levels by ELISA,immunohistochemistry method to detect the expression of GHR in callus tissue. Results: The shear rates of 30s-1,5s-1and 1s-1in the whole blood of the observation group were lower than those of the controlled group on the days 20 and 28after the administration,the difference was statistically significant(P<0. 05),and the serum GH,P,Ca and ALP levels in the 28-day of the observation group were higher than those in the controlled group(P<0. 05). After 20 and 28days,the GHR expression in the observation group was higher than that in the controlled group,plasma viscosity was lower than the controlled group,the difference was statistically significant(P<0. 05). Conclusion: Fuyuan Huoxue Decoction can increase the serum GH activity,reduce the degradation rate and increase the expression of GHR in callus tissue of rats with femoral fractures,and then accelerate the fracture healing.
Objective: To investigate the effect of Fuyuan Huoxue Decoction on serum GH and callus GH receptor expression in femoral fractures of rats. Methods: Thirty male SD rats were randomly divided into experimental group and controlled group.Rats were anesthetized by intraperitoneal injection of 10% chloral hydrate,the femur was cut off with low-speed dental drill,and the Kirschner wire was retrograde fxed modeling,the observation group rats were given Fuyuan Huoxue Decoction gavage,the controlled group was treated with an equal dose of saline,once a day for a total of 28 days,10days after administration,20 days,28days were selected 5rats were anesthetized and sacrificed. The automatic blood rheology testing instrument was used to detect the plasma viscosity and whole blood viscosity of rats at different shear rates 10,20 and 28days after the administration. The contents of serum phosphorus(P),calcium(Ca),ALP content,serum GH levels by ELISA,immunohistochemistry method to detect the expression of GHR in callus tissue. Results: The shear rates of 30s-1,5s-1and 1s-1in the whole blood of the observation group were lower than those of the controlled group on the days 20 and 28after the administration,the difference was statistically significant(P<0. 05),and the serum GH,P,Ca and ALP levels in the 28-day of the observation group were higher than those in the controlled group(P<0. 05). After 20 and 28days,the GHR expression in the observation group was higher than that in the controlled group,plasma viscosity was lower than the controlled group,the difference was statistically significant(P<0. 05). Conclusion: Fuyuan Huoxue Decoction can increase the serum GH activity,reduce the degradation rate and increase the expression of GHR in callus tissue of rats with femoral fractures,and then accelerate the fracture healing.
引文
[1]吴征杰,李灿辉,杨宇,等.活血益气法对大鼠股骨骨折后高凝血状态的影响[J].广州中医药大学学报,2017,34(2):231~235
[2] Aydin A,Halici Z,Akpinar E,et al. What is the role of bosentan in healing of femur fractures in a rat model?[J]. Journal of Bone Mineral Metabolism,2015,33(5):496~506
[3]王希强,孙仁光,孙仕润,等.丹参接骨胶囊对闭合性股骨骨折模型大鼠骨折愈合的影响[J].中国组织工程研究,2016,20(5):622~627
[4]柏小金,徐林,骆旭东,等.构建股骨骨折合并脑损伤模型大鼠低氧诱导因子1α和核心结合因子α1的表达[J].中国组织工程研究,2015,19(18):2800~2806
[5] Liu Y,Rui Y,Cheng T Y,et al. Effects of Sclerostin Antibody on the Healing of Femoral Fractures in Ovariectomised Rats.[J]. Calcified Tissue International,2015,98(3):263~274
[6]叶尚松,李红,毛显法,等.自助垂直牵引整复联合复元活血汤加减治疗单纯胸腰椎压缩性骨折的临床研究[J].中医药导报,2016,9(10):86~89
[7]肖增兵,陈通,付爱军,等.构建颅脑损伤合并股骨闭合性骨折大鼠模型:骨痂中降钙素基因相关肽和胰岛素样生长因子1的表达[J].中国组织工程研究,2015,19(5):657~661
[8]罗成斌,蒋春彦,朱珠,等.针刺对实验性骨质疏松大鼠股骨骨折的预防作用[J].中国骨质疏松杂志,2017,23(1):102~106
[9] Miyazaki M,Toyoda M,Yoshiiwa T,et al. Enhancement of the effects of exfoliated carbon nanofibers by bone morphogenetic protein in a rat femoral fracture model[J]. Journal of Orthopaedic Research,2015,33(2):185~192
[10]汪雷,刘林娟,肖黎,等.脊髓损伤合并股骨骨折大鼠模型中leptin的表达[J].实用医学杂志,2015,31(22):3688~3690
[11] Zangouri V,Khodaei S,Ketabchi F,et al. Effects of voluven on lung injury induced by hemorrhagic shock and femoral fracture in rat[J]. European Respiratory Journal,2015,46(9):3034~3037
[12]许立华,王桂仁,陈德监.复元活血汤治疗骨折延迟愈合临床疗效分析[J].山东中医杂志,2015,11(9):670~672
[13]程艳刚,谭金燕,荆然,等.复元活血汤临床应用及实验研究进展[J].辽宁中医药大学学报,2016,7(12):149~152
[14]孟凡彪,刘宝华,刘佐君.尾静脉移植高表达Aqp1基因的骨髓间充质干细胞对股骨骨折大鼠骨愈合的影响[J].山东医药,2017,19(48):25~28
[15] Apfgm G,Butezloff M M,Zamarioli A,et al. Nandrolone decanoate appears to increase bone callus formation in young adult rats after a complete femoral fracture[J]. Acta Cirurgica Brasileira, 2017, 32(11):924~927
[16]徐冰,郑军,汤伟忠.复元活血汤在骨折中的临床应用研究进展[J].中医药导报,2016,13(20):81~83
[17]周缜,蓝青,李洪波,等.壮筋续骨汤对骨折大鼠血清内源性生长因子水平的影响及意义[J].中国中西医结合外科杂志,2015,21(5):479~484
[18]陈怀春,钱霄,陈欣杰,等.复元活血汤防治早期胸腰椎压缩性骨折后腹胀便秘的临床体会[J].世界中医药,2016,5(6):1809~1809
[2] Aydin A,Halici Z,Akpinar E,et al. What is the role of bosentan in healing of femur fractures in a rat model?[J]. Journal of Bone Mineral Metabolism,2015,33(5):496~506
[3]王希强,孙仁光,孙仕润,等.丹参接骨胶囊对闭合性股骨骨折模型大鼠骨折愈合的影响[J].中国组织工程研究,2016,20(5):622~627
[4]柏小金,徐林,骆旭东,等.构建股骨骨折合并脑损伤模型大鼠低氧诱导因子1α和核心结合因子α1的表达[J].中国组织工程研究,2015,19(18):2800~2806
[5] Liu Y,Rui Y,Cheng T Y,et al. Effects of Sclerostin Antibody on the Healing of Femoral Fractures in Ovariectomised Rats.[J]. Calcified Tissue International,2015,98(3):263~274
[6]叶尚松,李红,毛显法,等.自助垂直牵引整复联合复元活血汤加减治疗单纯胸腰椎压缩性骨折的临床研究[J].中医药导报,2016,9(10):86~89
[7]肖增兵,陈通,付爱军,等.构建颅脑损伤合并股骨闭合性骨折大鼠模型:骨痂中降钙素基因相关肽和胰岛素样生长因子1的表达[J].中国组织工程研究,2015,19(5):657~661
[8]罗成斌,蒋春彦,朱珠,等.针刺对实验性骨质疏松大鼠股骨骨折的预防作用[J].中国骨质疏松杂志,2017,23(1):102~106
[9] Miyazaki M,Toyoda M,Yoshiiwa T,et al. Enhancement of the effects of exfoliated carbon nanofibers by bone morphogenetic protein in a rat femoral fracture model[J]. Journal of Orthopaedic Research,2015,33(2):185~192
[10]汪雷,刘林娟,肖黎,等.脊髓损伤合并股骨骨折大鼠模型中leptin的表达[J].实用医学杂志,2015,31(22):3688~3690
[11] Zangouri V,Khodaei S,Ketabchi F,et al. Effects of voluven on lung injury induced by hemorrhagic shock and femoral fracture in rat[J]. European Respiratory Journal,2015,46(9):3034~3037
[12]许立华,王桂仁,陈德监.复元活血汤治疗骨折延迟愈合临床疗效分析[J].山东中医杂志,2015,11(9):670~672
[13]程艳刚,谭金燕,荆然,等.复元活血汤临床应用及实验研究进展[J].辽宁中医药大学学报,2016,7(12):149~152
[14]孟凡彪,刘宝华,刘佐君.尾静脉移植高表达Aqp1基因的骨髓间充质干细胞对股骨骨折大鼠骨愈合的影响[J].山东医药,2017,19(48):25~28
[15] Apfgm G,Butezloff M M,Zamarioli A,et al. Nandrolone decanoate appears to increase bone callus formation in young adult rats after a complete femoral fracture[J]. Acta Cirurgica Brasileira, 2017, 32(11):924~927
[16]徐冰,郑军,汤伟忠.复元活血汤在骨折中的临床应用研究进展[J].中医药导报,2016,13(20):81~83
[17]周缜,蓝青,李洪波,等.壮筋续骨汤对骨折大鼠血清内源性生长因子水平的影响及意义[J].中国中西医结合外科杂志,2015,21(5):479~484
[18]陈怀春,钱霄,陈欣杰,等.复元活血汤防治早期胸腰椎压缩性骨折后腹胀便秘的临床体会[J].世界中医药,2016,5(6):1809~1809