精子碎片指数与精子形态的关系研究
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摘要
目的 :探讨精子DNA碎片指数(DNA fragmentation index,DFI)与精子形态学之间的相关性。方法 :回顾性分析3 068例男性患者的精液检查结果,将精子DFI分为<15%、15%~30%、>30%共3组,再根据精子形态学检查结果,按照正常形态精子所占比例<4%、4%~10%、>10%将精子分为3组,分析精子不同形态学分组与精子DFI分组间的关系。结果:不同精子DFI分组的正常形态、头部畸形、混合畸形百分比差异无统计学意义(P均>0.05);不同的精子正常形态率分组间的精子DFI差异无统计学意义(P均>0.05),用Spearson相关性分析精子DFI与精子形态之间的相关性,结果发现两者间无相关性。结论:本研究发现精子DFI与精子形态学之间没有相关性。
Objective: To study the correlation between sperm DNA fragmentation index(DFI) and sperm morphology in male semen. Methods: A total of 3 068 male subjects were enrolled and their semen samples were obtained and ana-lyzed. According to the sperm DFI, the semen samples were divided into three groups: DFI<15%, 15%-30%, >30%. Ac-cording to the percentage of spermatocytes with normal morphology, the sperm were divided into three groups: <4%, 4%-10%, > 10%. The relationship between DNA fragment index and sperm morphology in these groups was analyzed. Results:There was no significant difference in rates of normal morphology, head deformity and mixed deformity between groups with different sperm DFI index(P>0.05). There was no significant difference in sperm DFI between groups with different percen-tage of normal morphology of sperm. There was no significant correlation between different sperm normal morphol-ogy group and sperm DFI group(P>0.05). Conclusions: This study showed that there is no correlation between sperm DFI and sperm morphology.
Objective: To study the correlation between sperm DNA fragmentation index(DFI) and sperm morphology in male semen. Methods: A total of 3 068 male subjects were enrolled and their semen samples were obtained and ana-lyzed. According to the sperm DFI, the semen samples were divided into three groups: DFI<15%, 15%-30%, >30%. Ac-cording to the percentage of spermatocytes with normal morphology, the sperm were divided into three groups: <4%, 4%-10%, > 10%. The relationship between DNA fragment index and sperm morphology in these groups was analyzed. Results:There was no significant difference in rates of normal morphology, head deformity and mixed deformity between groups with different sperm DFI index(P>0.05). There was no significant difference in sperm DFI between groups with different percen-tage of normal morphology of sperm. There was no significant correlation between different sperm normal morphol-ogy group and sperm DFI group(P>0.05). Conclusions: This study showed that there is no correlation between sperm DFI and sperm morphology.
引文
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[13]Kruger TF,Acosta AA,Simmons KF,et al.Predictive value of abnormal sperm morphology in in vitro fertilization[J].Fertil Steril,1988,49(1):112-117.
[14]Zinaman MJ,Brown CC,Selevan SG,et al.Semen quality and human fertility:a prospective study with healthy couples[J].J Androl,2000,21(1):145-153.
[15]García-Vázquez FA,Gadea J,Matás C,et al.Importance of sperm morphology during sperm transport and fertilization in mammals[J].Asian J Androl,2016,18(6):844-850.
[16]Ko EY,Sabanegh ES Jr,Agarwal A.Male infertility testing:reactive oxygen species and antioxidant capacity[J].Fertil Steril,2014,102(6):1518-1527.
[17]Cissen M,Wely MV,Scholten I,et al.Measuring Sperm DNA Fragmentation and Clinical Outcomes of Medically Assisted Reproduction:A Systematic Review and MetaAnalysis[J].PLo S One,2016,11(11):e0165125.
[2]Marcon L,Boissonneault G.Transient DNA strand breaks during mouse and human spermiogenesis new insights in stage specificity and link to chromatin remodeling[J].Biol Reprod,2004,70(4):910-918.
[3]Cocuzza M,Sikka SC,Athayde KS,et al.Clinical relevance of oxidative stress and sperm chromatin damage in male infertility:an evidence based analysis[J].Int Braz J Urol,2007,33(5):603-621.
[4]Lin MH,Kuo-Kuang Lee R,Li SH,et al.Sperm chromatin structure assay parameters are not related to fertilization rates,embryo quality,and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection,but might be related to spontaneous abortion rates[J].Fertil Steril,2008,90(2):352-359.
[5]Cocuzza M,Sikka SC,Athayde KS,et al.Clinical relevance of oxidative stress and sperm chromatin damage in male infertility:an evidence based analysis[J].Int Braz J Urol,2007,33(5):603-621.
[6]Evenson D,Wixon R.Meta-analysis of sperm DNA fragmentation using the sperm chromatin structure assay[J].Reprod Biomed Online,2006,12(4):466-472.
[7]Lewis SE,John Aitken R,Conner SJ,et al.The impact of sperm DNA damage in assisted conception and beyond:recent advances in diagnosis and treatment[J].Reprod Biomed Online,2013,27(4):325-337.
[8]Smit M,Romijn JC,Wildhagen MF,et al.Decreased sperm DNA fragmentation after surgical varicocelectomy is associated with increased pregnancy rate[J].J Urol,2013,189(1 Suppl):S146-S150.
[9]Slama R,Eustache F,Ducot B,et al.Time to pregnancy and semen parameters:a cross-sectional study among fertile couples from four European cities[J].Hum Reprod,2002,17(2):503-515.
[10]Wang L,Zhang J,Duan J,et al.Programming and inheritance of parental DNA methylomes in mammals[J].Cell,2014,157(4):979-991.
[11]Donnelly ET,Lewis SE,Mc Nally JA,et al.In vitro fertilization and pregnancy rates:the influence of sperm motility and morphology on IVF outcome[J].Fertil Steril,1998,70(2):305-314.
[12]Cao X,Cui Y,Zhang X,et al.The correlation of sperm morphology with unexplained recurrent spontaneous abortion:A systematic review and meta-analysis[J].Oncotarget,2017,8(33):55646-55656.
[13]Kruger TF,Acosta AA,Simmons KF,et al.Predictive value of abnormal sperm morphology in in vitro fertilization[J].Fertil Steril,1988,49(1):112-117.
[14]Zinaman MJ,Brown CC,Selevan SG,et al.Semen quality and human fertility:a prospective study with healthy couples[J].J Androl,2000,21(1):145-153.
[15]García-Vázquez FA,Gadea J,Matás C,et al.Importance of sperm morphology during sperm transport and fertilization in mammals[J].Asian J Androl,2016,18(6):844-850.
[16]Ko EY,Sabanegh ES Jr,Agarwal A.Male infertility testing:reactive oxygen species and antioxidant capacity[J].Fertil Steril,2014,102(6):1518-1527.
[17]Cissen M,Wely MV,Scholten I,et al.Measuring Sperm DNA Fragmentation and Clinical Outcomes of Medically Assisted Reproduction:A Systematic Review and MetaAnalysis[J].PLo S One,2016,11(11):e0165125.