中国工厂化主栽双孢蘑菇品种的SSR标记遗传多样性分析
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摘要
通过33对SSR引物对7份双孢蘑菇栽培菌株基因组DNA进行PCR扩增,共检测出62个等位基因,每对引物的等位基因数为2～4个,平均为2.48个。其中25对引物在7个双孢蘑菇基因型中存在明显多态性,占检测引物总数的75.76%。SSR引物仅能将7份菌菇材料区分为4类。其中,国内褐色双孢蘑菇主栽菌株棕秀一号、LY、LC的遗传相似系数为1,与国外褐色双孢蘑菇菌株MG遗传相似系数为0.645 2。国内褐色双孢蘑菇与国外褐色双孢蘑菇菌株MG遗传背景存在差异,国内褐色双孢蘑菇菌株具有相似的遗传背景。结果解释了国内褐色双孢蘑菇菌株遗传背景狭窄,多样性差,选育褐色双孢蘑菇优良栽培菌种具有非常现实的意义。
33 pairs of SSR primers were used to amplify the genomic DNA of 7 kinds of industrial leading cultivated A. bisporus strains, and 62 alleles were detected. The number of alleles in each pair of primers was 2～4, and the average number was 2.48. 25 pairs of primers showed obvious polymorphism in 7 A. bisporus strains genotype,which occupied 75.76% of the detected primers numbers. 7 kinds of A. bisporus strains could be classified into 4 genetic groups by SSR primers. The genetic similarity coefficient was 1 for Zongxiu No.1, LY and LC, and the genetic similarity coefficient was 0.645 2 between Zongxiu No.1 and MG. There were differences in genetic background between domestic and foreign strains of Agaricus bisporus. While domestic strains of Agaricus bisporus had similar genetic background. The results explained the narrow genetic background and poor diversity of the domestic strains of Agaricus bisporus, which was very practical to the selection of the best cultivated strains of Agaricus bisporus.
33 pairs of SSR primers were used to amplify the genomic DNA of 7 kinds of industrial leading cultivated A. bisporus strains, and 62 alleles were detected. The number of alleles in each pair of primers was 2～4, and the average number was 2.48. 25 pairs of primers showed obvious polymorphism in 7 A. bisporus strains genotype,which occupied 75.76% of the detected primers numbers. 7 kinds of A. bisporus strains could be classified into 4 genetic groups by SSR primers. The genetic similarity coefficient was 1 for Zongxiu No.1, LY and LC, and the genetic similarity coefficient was 0.645 2 between Zongxiu No.1 and MG. There were differences in genetic background between domestic and foreign strains of Agaricus bisporus. While domestic strains of Agaricus bisporus had similar genetic background. The results explained the narrow genetic background and poor diversity of the domestic strains of Agaricus bisporus, which was very practical to the selection of the best cultivated strains of Agaricus bisporus.
引文
Cai Z.X.,Chen M.Y.,Liao J.H.,Li H.R.,Guo Z.J.,Cai D.F.,and Wang Z.S.,2014,Primary screening of molecular markers relates to fruit body color in Agaricus bisprus,Shiyongjun Xuebao(Acta Edulis Fungi),21(3):1-5(蔡志欣,陈美元,廖剑华,李洪荣,郭仲杰,蔡丹凤,王泽生,2014,双孢蘑菇颜色基因分子标记的初步筛选,食用菌学报,21(3):1-5)
Gu M.,2014,Development and application of SSR markers in Agaricus bisporus,Thesis for M.S.,Zhejiang Normal Uni versity,Supervisor:Chen M.J.,pp.22-28(顾敏,2014,双孢蘑菇SSR标记的开发及其在遗传育种分析中的应用,硕士学位论文,浙江师范大学,导师:陈明杰,pp.22-28)
Lai Y.,Wang P.X.,Fan G.Q.,Si E.J.,Wang J.,Yang K.,Meng Y.X.,Li B.C.,Ma X.L.,Shang X.W.,and Wang H.J.,2013,Genetic diversity and association analysis using SSR markers in barley,Zhonguo Nongye Kexue(Scientia Agricultura Sinica),46(2):233-242(赖勇,王鹏喜,范贵强,司二静,王晋,杨轲,孟亚雄,李葆春,马小乐,尚勋武,王化俊,2013,大麦SSR标记遗传多样性及其与农艺性状关联分析,中国农业科学,46(2):233-242)
Li G.G.,Zhang H.,Zheng Y.S.,and Li R.H.,2018,The genetic diversity analysis of Chinese flowering cabbage resources based on SSR marker,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),37(3):1257-1264(李光光,张华,郑岩松,李荣华,2018,利用SSR标记研究菜心资源的遗传多样性分析,基因组学与应用生物学,37(3):1257-1264)
Li J.W.,Wen G.H.,Li G.F.,Zhang R.,Ma S.,Qi E.F.,and Jia X.X.,2017,SSR analysis of genetic diversity for leading potato varieties in Gansu Province,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(5):1951-1962(李建武,文国宏,李高峰,张荣,马胜,齐恩芳,贾小霞,2017,甘肃省主栽马铃薯品种的SSR遗传多样性分析,分子植物育种,15(5):1951-1962)
Li Y.L.,Yang X.X.,Zhang J.Y.,Huang S.W.,and Xiong X.Y.,2014,Studies on SSR molecular markers based on transcriptome of Taxus chinensis var.mairei,Yuanyi Xuebao(Acta Horticulturae Sinica),41(4):735-745(李炎林,杨星星,张家银,黄三文,熊兴耀,2014,南方红豆杉转录组SSR挖掘及分子标记的研究,园艺学报,41(4):735-745)
Liu D.Y.,Wang X.H.,Liu Y.,Zhang J.,and Chen H.Y.,2014,Analysis of genetic diversity and relationship of pakchoi accessions based on SSR markers,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(3):499-508(刘冬媛,王新华,刘杨,张峻,陈火英,2014,不结球白菜品种(系)遗传多样性与亲缘关系的SSR分析,分子植物育种,12(3):499-508)
Liu X.L.,Qu C.M.,Xie J.M.,Lu K.,Wang M.,Wang R.,Xu X.F.,Shen L.,and Li J.N.,2012,Genetic diversity of yellow-seeded parental lines of Brassica napus using SSR markers,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),13(4):632-638(刘晓兰,曲存民,谢景梅,卢坤,王敏,王瑞,徐新福,谌利,李加纳,2012,SSR标记对不同黄子甘蓝型油菜亲本材料的遗传分析,植物遗传资源学报,13(4):632-638)
Lu H.,Zhang D.,Zhang L.J.,Wang R.J.,Shang X.D.,and Tan Q.,2015,Association analysis of five agronomic traits with SSRmarkers in Flammulina velutipes germplasm,Nongye Shengwu Jishu Xuebao(Journal of Agricultural Biotechnology),23(1):96-106(陆欢,张丹,章炉军,王瑞娟,尚晓冬,谭琦,2015,金针菇种质资源5个农艺性状与SSR标记的关联分析,农业生物技术学报,23(1):96-106)
Morin E.,Kohler A.,Baker A.R.,Foulongne-Oriol M.,Lombard V.,Nagy L.G.,Ohm R.A.,Patyshakuliyeva A.,Brun A.,Aerts A.L.,Bailey A.M.,Billette C.,Coutinho P.M.,Deakin G.,Doddapaneni H.,Floudas D.,Grimwood J.,Hildén K.,Kües U.,Labutti K.M.,Lapidus A.,Lindquist E.A.,Lucas S.M.,Murat C.,Riley R.W.,Salamov A.A.,Schmutz J.,Subramanian V.,W觟sten H.A.,Xu J.,Eastwood D.C.,Foster G.D.,Sonnenberg A.S.,Cullen D.,De Vries R.P.,Lundell T.,Hibbett D.S.,Henrissat B.,Burton K.S.,Kerrigan R.W.,Challen M.P.,Grigoriev I.V.,and Martin F.,2012,Genome sequence of the button mushroom Agaricus bisporus reveals mechanisms governing adaptation to a humic-rich ecological niche,Proc.Natl.Acad.Sci.USA,109(43):17501-17506
Parthiban S.,Govindaraj P.,and Senthilkumar S.,2018,Comparison of relative efficiency of genomic SSR and EST-SSRmarkers in estimating genetic diversity in sugarcane,Biotech,8(3):144
Pu Y.Y.,Cheng K.,and Li S.S.,2011,Genetic diversity of recent wheat cultivars in Shandong Province using SSR markers,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),9(4):443-449(蒲艳艳,程凯,李斯深,2011,山东省近期育成小麦品种遗传多样性的SSR分析,分子植物育种,9(4):443-449)
Sun B.R.,Fu C.Y.,Yang C.Y.,Ma Q.B.,Pan D.J.,and Nian H.,2013,Genetic diversity of wild soybeans from some regions of southern China based on SSR and SRAP markers,Am.J.Plant Sci.,4(2):257-268
Wang F.G.,Tian H.L.,Zhao J.R.,Wang L.,Yi H.M.,Song W.,Gao Y.Q.,and Yang G.H.,2014,Genetic diversity analysis of 328 maize varieties(Hybridized combinations)using SSRmarkers,Zhonguo Nongye Kexue(Scientia Agricultura Sinica),47(5):856-864(王凤格,田红丽,赵久然,王璐,易红梅,宋伟,高玉倩,杨国航,2014,中国328个玉米品种(组合)SSR标记遗传多样性分析,中国农业科学,47(5):856-864)
Wang J.H.,Luo W.Y.,Chen J.W.,Liu Y.F.,Wu Q.H.,and Li X.F.,2005,Genetic diversity in cultivated fragrance rice varieties revealed by SSR and ISSR markers,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),3(1):37-34(王金花,罗文永,陈建伟,刘元风,吴秋花,李晓方,2005,应用SSR和ISSR标记分析栽培香稻品种的遗传多样性,分子植物育种,3(1):37-42)
Wang X.X.,Li D.,Song B.,Guo Y.X.,Su W.Y.,Dai Y.T.,Liu Y.,Fu Y.P.,and Li Y.,2016,Development of a single sequence repeat-based molecular ID system for differentiating Agaricus bisporus strains,Shiyongjun Xuebao(Acta Edulis Fungi),23(2):6-11(王新新,李丹,宋冰,郭玉秀,苏文英,戴月婷,刘源,付永平,李玉,2016,双孢蘑菇种质SSR分子身份证的构建,食用菌学报,23(2):6-11)
Wang Z.S.,Liao J.H.,Chen M.Y.,and Cai Z.X.,2012,Breeding industrial development of Agaricus bisporus,Shiyongjun Xuebao(Acta Edulis Fungi),19(3):1-14(王泽生,廖剑华,陈美元,蔡志欣,2012,双孢蘑菇遗传育种和产业发展,食用菌学报,19(3):1-14)
Wu H.L.,Wang X.H.,He L.L.,and Li F.S.,2014,Genetic diversity analysis of yunnan sugarcane cultivars on SSR marker,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(1):144-151(吴海兰,王先宏,何丽莲,李富生,2014,基于SSR标记的云南甘蔗栽培品种遗传多样性分析,分子植物育种,12(1):144-151)
Xu R.R.,and Tang Z.X.,2014,Genetic diversity of peanut landraces in Fujian Province,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(6):1235-1242(徐日荣,唐兆秀,2014,福建省花生地方品种的遗传多样性研究,分子植物育种,12(6):1235-1242)
Yang W.Z.,and Jiao Y.,2012,Application of SSR molecular marker technique in the field of biogenetics,Anhui Nongye Kexue(Journal of Anhui Agricultura Science),40(2):640-642(杨文柱,焦燕,2012,SSR分子标记技术在生物遗传学领域的应用,安徽农业科学,40(2):640-642)
Yang X.,Liu F.,Zhang Y.,Cheng Y.F.,Xue L.B.,and Chen X.H.,2016,Study on the genetic diversity of eggplant germplasm with SSR markers,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(12):3450-3457(杨旭,刘飞,张宇,成玉富,薛林宝,陈学好,2016,利用SSR标记研究茄子种质资源遗传多样性,基因组学与应用生物学,35(12):3450-3457)
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Zhang D.,Wu P.,Zhang L.J.,Tang L.H.,Song C.Y.,Shang X.D.,Bao D.P.,and Tan Q.,2012,Polymorphism of SSR markers based on the whole genome sequence of Lentinula edodes and use in strain identification,Shiyongjun Xuebao(Acta Edulis Fungi),19(4):1-6(张丹,巫萍,章炉军,唐利华,宋春艳,尚晓冬,鲍大鹏,谭琦,2012,基于香菇全基因组序列开发的部分SSR标记多态性分析与品种鉴定初探,食用菌学报,19(4):1-6)
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Zhao Y.X.,Gao Q.,Sun S.Y.,Wu Y.X.,Zhang R.X.,and Hao W.S.,2016,ISSR analysis on genetic diversity of different varieties(lines)in Solanum tuberosum L.,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(12):3430-3435(赵永秀,高茜,孙淑英,吴玉仙,张瑞霞,郝文胜,2016,马铃薯不同品种(系)遗传多样性的ISSR分析,分子植物育种,14(12):3430-3435)
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Gu M.,2014,Development and application of SSR markers in Agaricus bisporus,Thesis for M.S.,Zhejiang Normal Uni versity,Supervisor:Chen M.J.,pp.22-28(顾敏,2014,双孢蘑菇SSR标记的开发及其在遗传育种分析中的应用,硕士学位论文,浙江师范大学,导师:陈明杰,pp.22-28)
Lai Y.,Wang P.X.,Fan G.Q.,Si E.J.,Wang J.,Yang K.,Meng Y.X.,Li B.C.,Ma X.L.,Shang X.W.,and Wang H.J.,2013,Genetic diversity and association analysis using SSR markers in barley,Zhonguo Nongye Kexue(Scientia Agricultura Sinica),46(2):233-242(赖勇,王鹏喜,范贵强,司二静,王晋,杨轲,孟亚雄,李葆春,马小乐,尚勋武,王化俊,2013,大麦SSR标记遗传多样性及其与农艺性状关联分析,中国农业科学,46(2):233-242)
Li G.G.,Zhang H.,Zheng Y.S.,and Li R.H.,2018,The genetic diversity analysis of Chinese flowering cabbage resources based on SSR marker,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),37(3):1257-1264(李光光,张华,郑岩松,李荣华,2018,利用SSR标记研究菜心资源的遗传多样性分析,基因组学与应用生物学,37(3):1257-1264)
Li J.W.,Wen G.H.,Li G.F.,Zhang R.,Ma S.,Qi E.F.,and Jia X.X.,2017,SSR analysis of genetic diversity for leading potato varieties in Gansu Province,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(5):1951-1962(李建武,文国宏,李高峰,张荣,马胜,齐恩芳,贾小霞,2017,甘肃省主栽马铃薯品种的SSR遗传多样性分析,分子植物育种,15(5):1951-1962)
Li Y.L.,Yang X.X.,Zhang J.Y.,Huang S.W.,and Xiong X.Y.,2014,Studies on SSR molecular markers based on transcriptome of Taxus chinensis var.mairei,Yuanyi Xuebao(Acta Horticulturae Sinica),41(4):735-745(李炎林,杨星星,张家银,黄三文,熊兴耀,2014,南方红豆杉转录组SSR挖掘及分子标记的研究,园艺学报,41(4):735-745)
Liu D.Y.,Wang X.H.,Liu Y.,Zhang J.,and Chen H.Y.,2014,Analysis of genetic diversity and relationship of pakchoi accessions based on SSR markers,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(3):499-508(刘冬媛,王新华,刘杨,张峻,陈火英,2014,不结球白菜品种(系)遗传多样性与亲缘关系的SSR分析,分子植物育种,12(3):499-508)
Liu X.L.,Qu C.M.,Xie J.M.,Lu K.,Wang M.,Wang R.,Xu X.F.,Shen L.,and Li J.N.,2012,Genetic diversity of yellow-seeded parental lines of Brassica napus using SSR markers,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),13(4):632-638(刘晓兰,曲存民,谢景梅,卢坤,王敏,王瑞,徐新福,谌利,李加纳,2012,SSR标记对不同黄子甘蓝型油菜亲本材料的遗传分析,植物遗传资源学报,13(4):632-638)
Lu H.,Zhang D.,Zhang L.J.,Wang R.J.,Shang X.D.,and Tan Q.,2015,Association analysis of five agronomic traits with SSRmarkers in Flammulina velutipes germplasm,Nongye Shengwu Jishu Xuebao(Journal of Agricultural Biotechnology),23(1):96-106(陆欢,张丹,章炉军,王瑞娟,尚晓冬,谭琦,2015,金针菇种质资源5个农艺性状与SSR标记的关联分析,农业生物技术学报,23(1):96-106)
Morin E.,Kohler A.,Baker A.R.,Foulongne-Oriol M.,Lombard V.,Nagy L.G.,Ohm R.A.,Patyshakuliyeva A.,Brun A.,Aerts A.L.,Bailey A.M.,Billette C.,Coutinho P.M.,Deakin G.,Doddapaneni H.,Floudas D.,Grimwood J.,Hildén K.,Kües U.,Labutti K.M.,Lapidus A.,Lindquist E.A.,Lucas S.M.,Murat C.,Riley R.W.,Salamov A.A.,Schmutz J.,Subramanian V.,W觟sten H.A.,Xu J.,Eastwood D.C.,Foster G.D.,Sonnenberg A.S.,Cullen D.,De Vries R.P.,Lundell T.,Hibbett D.S.,Henrissat B.,Burton K.S.,Kerrigan R.W.,Challen M.P.,Grigoriev I.V.,and Martin F.,2012,Genome sequence of the button mushroom Agaricus bisporus reveals mechanisms governing adaptation to a humic-rich ecological niche,Proc.Natl.Acad.Sci.USA,109(43):17501-17506
Parthiban S.,Govindaraj P.,and Senthilkumar S.,2018,Comparison of relative efficiency of genomic SSR and EST-SSRmarkers in estimating genetic diversity in sugarcane,Biotech,8(3):144
Pu Y.Y.,Cheng K.,and Li S.S.,2011,Genetic diversity of recent wheat cultivars in Shandong Province using SSR markers,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),9(4):443-449(蒲艳艳,程凯,李斯深,2011,山东省近期育成小麦品种遗传多样性的SSR分析,分子植物育种,9(4):443-449)
Sun B.R.,Fu C.Y.,Yang C.Y.,Ma Q.B.,Pan D.J.,and Nian H.,2013,Genetic diversity of wild soybeans from some regions of southern China based on SSR and SRAP markers,Am.J.Plant Sci.,4(2):257-268
Wang F.G.,Tian H.L.,Zhao J.R.,Wang L.,Yi H.M.,Song W.,Gao Y.Q.,and Yang G.H.,2014,Genetic diversity analysis of 328 maize varieties(Hybridized combinations)using SSRmarkers,Zhonguo Nongye Kexue(Scientia Agricultura Sinica),47(5):856-864(王凤格,田红丽,赵久然,王璐,易红梅,宋伟,高玉倩,杨国航,2014,中国328个玉米品种(组合)SSR标记遗传多样性分析,中国农业科学,47(5):856-864)
Wang J.H.,Luo W.Y.,Chen J.W.,Liu Y.F.,Wu Q.H.,and Li X.F.,2005,Genetic diversity in cultivated fragrance rice varieties revealed by SSR and ISSR markers,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),3(1):37-34(王金花,罗文永,陈建伟,刘元风,吴秋花,李晓方,2005,应用SSR和ISSR标记分析栽培香稻品种的遗传多样性,分子植物育种,3(1):37-42)
Wang X.X.,Li D.,Song B.,Guo Y.X.,Su W.Y.,Dai Y.T.,Liu Y.,Fu Y.P.,and Li Y.,2016,Development of a single sequence repeat-based molecular ID system for differentiating Agaricus bisporus strains,Shiyongjun Xuebao(Acta Edulis Fungi),23(2):6-11(王新新,李丹,宋冰,郭玉秀,苏文英,戴月婷,刘源,付永平,李玉,2016,双孢蘑菇种质SSR分子身份证的构建,食用菌学报,23(2):6-11)
Wang Z.S.,Liao J.H.,Chen M.Y.,and Cai Z.X.,2012,Breeding industrial development of Agaricus bisporus,Shiyongjun Xuebao(Acta Edulis Fungi),19(3):1-14(王泽生,廖剑华,陈美元,蔡志欣,2012,双孢蘑菇遗传育种和产业发展,食用菌学报,19(3):1-14)
Wu H.L.,Wang X.H.,He L.L.,and Li F.S.,2014,Genetic diversity analysis of yunnan sugarcane cultivars on SSR marker,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(1):144-151(吴海兰,王先宏,何丽莲,李富生,2014,基于SSR标记的云南甘蔗栽培品种遗传多样性分析,分子植物育种,12(1):144-151)
Xu R.R.,and Tang Z.X.,2014,Genetic diversity of peanut landraces in Fujian Province,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(6):1235-1242(徐日荣,唐兆秀,2014,福建省花生地方品种的遗传多样性研究,分子植物育种,12(6):1235-1242)
Yang W.Z.,and Jiao Y.,2012,Application of SSR molecular marker technique in the field of biogenetics,Anhui Nongye Kexue(Journal of Anhui Agricultura Science),40(2):640-642(杨文柱,焦燕,2012,SSR分子标记技术在生物遗传学领域的应用,安徽农业科学,40(2):640-642)
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