邻苯二甲酸酯类污染物雌激素结合活性筛查
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摘要
以倏逝波光纤荧光生物传感器为平台,以邻苯二甲酸酯类(PAEs)污染物为靶标,优化了基于受体作用理论的生物传感分析技术,实现了PAEs雌激素结合活性的定性筛查。对7种典型邻苯二甲酸酯的雌激素结合活性测试结果为BBP>DBP>DIPP,其他4种物质几乎无雌激素结合活性,结果与文献广泛报道结论相一致,验证了所建立方法的准确性。在最优测试条件下,光纤传感界面可再生300次以上,为雌激素活性污染物的筛查提供了一种低成本、自动化方法。
Taking the evanescent-wave optical fiber fluorescent biosensor as a platform and the phthalic acid esters(PAEs)contaminants as targets,the biosensing analytical technology based on the estrogen receptor interaction theory was optimized,and qualitativescreening of estrogen binding activity of PAEs was achieved. The order of the estrogenic binding activity of seven typical PAEs were BBP >DBP > DIPP. The other 4substances had almost no estrogenic binding activity,which was consistent with the widely reported conclusions inLiteratures,and confirming the accuracy of the established method. Under the optimal test conditions,the optical fiber sensing surface can beregenerated more than 300 times,providing a Low-cost,automated method for the screening of estrogenic contaminants.
Taking the evanescent-wave optical fiber fluorescent biosensor as a platform and the phthalic acid esters(PAEs)contaminants as targets,the biosensing analytical technology based on the estrogen receptor interaction theory was optimized,and qualitativescreening of estrogen binding activity of PAEs was achieved. The order of the estrogenic binding activity of seven typical PAEs were BBP >DBP > DIPP. The other 4substances had almost no estrogenic binding activity,which was consistent with the widely reported conclusions inLiteratures,and confirming the accuracy of the established method. Under the optimal test conditions,the optical fiber sensing surface can beregenerated more than 300 times,providing a Low-cost,automated method for the screening of estrogenic contaminants.
引文
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[6]Jobling S, Reynolds T, White R, et al. A variety of environmentallypersistent chemicals, including some phthalate plasticizers, areweakly estrogenic[J]. Environ Health Perspect, 1995, 103(6):582-587.
[7]Takeuchi S, Iida M, Kobayashi S, et al. Differential effects ofphthalate esters on transcriptional activities via human estrogenreceptorsαandβ, and androgen receptor[J]. Toxicology, 2005,210(2):223-233.
[8]Carrillo JD, Salazar C, Moreta C, et al. Determination of phthalatesin wine by headspace solid-phase microextraction followed bygas chromatography-mass spectrometry:fibre comparison andselection[J]. J Chromatogr A, 2007, 1164(1-2):248-261.
[9]Fang GZ, He JX, Wang S. Multiwalled carbon nanotubes as sorbentfor on-Line coupling of solid-phase extraction to high-performanceliquid chromatography for simultaneous determination of 10sulfonamides in eggs and pork[J]. J Chromatogr A, 2006, 1127(1-2):12-17.
[10]Speltini A, Sturini M, Maraschi F, et al. Graphene-derivatized silicaas an efficient solid-phase extraction sorbent for pre-concentrationof fluoroquinolones from water followed by liquid-chromatographyfluorescence detection[J]. J Chromatogr A, 2015, 1379:9-15.
[11]Kuang H, Xu L, Cui G, et al. Development of determination of din-octyl phthalate(DOP)residue by an indirect enzyme-Linkedimmunosorbent assay[J]. Food and Agricultural Immunology,2010, 21(3):265-277.
[12]Li N, Wang D, Zhou Y, et al. Dibutyl phthalate contributes to thethyroid receptor antagonistic activity in drinking water processes[J]. Environ Sci Technol, 2010, 44(17):6863-6868.
[13]Takenouchi O, Kanno A, Takakura H, et al. A bioluminescentindicator for highly sensitive analysis of estrogenic activity in acell-based format[J]. Bioconjug Chem, 2016, 27(11):2689.
[14]Liu L, Zhou X, Lu Y, et al. Facile screening of potential xenoestrogens by an estrogen receptor-based reusable optical biosensor[J].Biosensors and Bioelectronics, 2017, 97:16-20.
[2]Meeker JD, Sathyanarayana S, Swan SH. Phthalates and otheradditives in plastics:human exposure and associated health outcomes[J]. Philos Trans R Soc Lond B Biol Sci, 2009, 64(1526):2097-2113.
[3]EUA. Department of Health and Human Services. Agency for toxicsubstances and disease registry. Toxicological profile for di-noctylphthalate[J]. Atsdr Toxicological Profile, 1997.
[4]EUA. Department of Health and Human Services. Agency for toxicsubstances and disease registry. Toxicological profile for di-n-butylphthalate[J]. Atsdr Toxicological Profile, 2001, 18(3):187-191.
[5]Heudorf U, Mersch-Sundermann V, Angerer J. Phthalates:Toxicology and exposure[J]. Int J Hyg Environ Health, 2007, 210(5):623-634.
[6]Jobling S, Reynolds T, White R, et al. A variety of environmentallypersistent chemicals, including some phthalate plasticizers, areweakly estrogenic[J]. Environ Health Perspect, 1995, 103(6):582-587.
[7]Takeuchi S, Iida M, Kobayashi S, et al. Differential effects ofphthalate esters on transcriptional activities via human estrogenreceptorsαandβ, and androgen receptor[J]. Toxicology, 2005,210(2):223-233.
[8]Carrillo JD, Salazar C, Moreta C, et al. Determination of phthalatesin wine by headspace solid-phase microextraction followed bygas chromatography-mass spectrometry:fibre comparison andselection[J]. J Chromatogr A, 2007, 1164(1-2):248-261.
[9]Fang GZ, He JX, Wang S. Multiwalled carbon nanotubes as sorbentfor on-Line coupling of solid-phase extraction to high-performanceliquid chromatography for simultaneous determination of 10sulfonamides in eggs and pork[J]. J Chromatogr A, 2006, 1127(1-2):12-17.
[10]Speltini A, Sturini M, Maraschi F, et al. Graphene-derivatized silicaas an efficient solid-phase extraction sorbent for pre-concentrationof fluoroquinolones from water followed by liquid-chromatographyfluorescence detection[J]. J Chromatogr A, 2015, 1379:9-15.
[11]Kuang H, Xu L, Cui G, et al. Development of determination of din-octyl phthalate(DOP)residue by an indirect enzyme-Linkedimmunosorbent assay[J]. Food and Agricultural Immunology,2010, 21(3):265-277.
[12]Li N, Wang D, Zhou Y, et al. Dibutyl phthalate contributes to thethyroid receptor antagonistic activity in drinking water processes[J]. Environ Sci Technol, 2010, 44(17):6863-6868.
[13]Takenouchi O, Kanno A, Takakura H, et al. A bioluminescentindicator for highly sensitive analysis of estrogenic activity in acell-based format[J]. Bioconjug Chem, 2016, 27(11):2689.
[14]Liu L, Zhou X, Lu Y, et al. Facile screening of potential xenoestrogens by an estrogen receptor-based reusable optical biosensor[J].Biosensors and Bioelectronics, 2017, 97:16-20.