重组自交系稻米品质性状的相关性分析及QTL定位
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摘要
【目的】分析重组自交系稻米品质性状的相关性,并对其进行数量性状座位(QTL)定位,为水稻品质性状遗传改良提供理论依据。【方法】以珍汕97B、柳沙油占202及二者杂交构建的174个重组自交系群体为材料,对12个稻米品质性状进行相关性分析和QTL定位。【结果】3个稻米碾磨品质性状(出糙率、精米率和整精米率)间均呈极显著正相关(P<0.01,下同)。6个稻米外观品质性状中,除粒长与垩白率、垩白度和透明度间无显著相关性(P>0.05)外,其他性状间均呈极显著正或负相关。3个稻米蒸煮食味品质性状中,胶稠度与直链淀粉含量和碱消值呈极显著负相关。碾磨品质性状、外观品质性状和蒸煮食味品质性状间也存在不同程度相关性,如垩白粒率和垩白度与除粒长外的其他9个性状呈显著(P<0.05,下同)或极显著相关等。在第5和第6染色体上共检测出出糙率、精米率、粒宽、长宽比、垩白率、垩白度、直链淀粉含量、胶稠度和碱消值9个性状的11个QTL,其中9个QTL的表型变异贡献率大于10%,为主效QTL,且显著或极显著相关性状的QTL定位区域存在趋同现象。【结论】重组自交系群体12个稻米品质性状存在互作效应,与QTL定位结果相互印证。鉴定所得主效QTL可用于水稻稻米品质相关性状的遗传改良。
【Objective】In the present study,correlation analysis of rice recombinant inbred line was studied,and quantitative trait loci mapping was carried out to provide reference for rice quality traits genetic improvement.【Method】One hundred and seventy-four recombinant inbred line(RIL)populations derived from the cross between Zhenshan 97 B and Liushayouzhan 202 were used to conduct correlation analysis and QTL mapping of rice quality traits.【Result】The results showed that three traits of milling,namely percentage of brown rice,milled rice rate and head rice rate had extremely significant correlation between each other(P<0.01,the same below). Among the six appearance traits,except that grain length had no significant correlation with chalky grain rate,chalkiness and transparency(P>0.05),the other traits had extremely positive correlation or extremely negative correlation between one and another. In the three cooking and eating quality traits,gel consistency had extremely negative correlation with amylase content and gelatinization temperature.Milling quality traits,appearance quality traits,cooking and eating quality traits also had correlation at various degrees.For example,chalky grain rate and chalkiness was significantly(P<0.05,the same below)or extremely correlated with all the traits except grain length. For the nine traits(percentage of brown rice,milled rice rate,grain width,ratio of length to width,chalky grain rate,chalkiness,amylase content,gel consistency and gelatinization temperature),eleven QTLs were identified locating on chromosome 5 and chromosome 6. There were nine QTLs whose phenotypic variation contribution rates were over 10%,they were major effects QTLs. The QTLs location area of the traits which had significant or extremely significant correlation between each other were apt to be located in the same region.【Conclusion】There are interaction effects among the 12 grain quality-related traits tested on recombinant inbred line populations. Such effects can be testified in the further QTL mapping detecting. Major QTLs detected in the present study can be used in genetic improvement of rice quality-related traits.
【Objective】In the present study,correlation analysis of rice recombinant inbred line was studied,and quantitative trait loci mapping was carried out to provide reference for rice quality traits genetic improvement.【Method】One hundred and seventy-four recombinant inbred line(RIL)populations derived from the cross between Zhenshan 97 B and Liushayouzhan 202 were used to conduct correlation analysis and QTL mapping of rice quality traits.【Result】The results showed that three traits of milling,namely percentage of brown rice,milled rice rate and head rice rate had extremely significant correlation between each other(P<0.01,the same below). Among the six appearance traits,except that grain length had no significant correlation with chalky grain rate,chalkiness and transparency(P>0.05),the other traits had extremely positive correlation or extremely negative correlation between one and another. In the three cooking and eating quality traits,gel consistency had extremely negative correlation with amylase content and gelatinization temperature.Milling quality traits,appearance quality traits,cooking and eating quality traits also had correlation at various degrees.For example,chalky grain rate and chalkiness was significantly(P<0.05,the same below)or extremely correlated with all the traits except grain length. For the nine traits(percentage of brown rice,milled rice rate,grain width,ratio of length to width,chalky grain rate,chalkiness,amylase content,gel consistency and gelatinization temperature),eleven QTLs were identified locating on chromosome 5 and chromosome 6. There were nine QTLs whose phenotypic variation contribution rates were over 10%,they were major effects QTLs. The QTLs location area of the traits which had significant or extremely significant correlation between each other were apt to be located in the same region.【Conclusion】There are interaction effects among the 12 grain quality-related traits tested on recombinant inbred line populations. Such effects can be testified in the further QTL mapping detecting. Major QTLs detected in the present study can be used in genetic improvement of rice quality-related traits.
引文
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郭桂英,王青林,马汉云,鲁伟林,沈光辉,全瑞兰,余新春,扶定,霍二伟.2017.碾磨品质对籼稻食味品质的影响[J].天津农业科学,23(6):40-44.[Guo G Y,Wang Q L,Ma H Y,Lu W L,Shen G H,Quan R L,Yu X C,Fu D,Huo E W.2017.Effect of milling quality on eating quality of indica rice[J].Tianjin Agricultural Sciences,23(6):40-44.]
胡霞,石瑜敏,贾倩,徐琴,王韵,陈凯,孙勇,朱苓华,徐建龙,黎志康.2011.影响水稻穗部性状及籽粒碾磨品质的QTL及其环境互作分析[J].作物学报,37(7):1175-1185.[Hu X,Shi Y M,Jia Q,Xu Q,Wang Y,Chen K,Sun Y,Zhu L H,Xu J L,Li Z K.2011.Analyses of QTLs for rice panicle and milling quality traits and their interaction with environment[J].Acta Agronomica Sinica,37(7):1175-1185.]
黄发松,孙宗修,胡培松,唐绍清.1998.食用稻米品质形成研究的现状与展望[J].中国水稻科学,12(3):172-176.[Huang F S,Sun Z X,Hu P S,Tang S Q.1998.Present situations and prospects for the research on rice grain quality forming[J].Chinese Journal of Rice Science,12(3):172-176.]
刘家富,奎丽梅,朱作峰,谭禄宾,王桂娟,黎其万,束继红,孙传清.2007.普通野生稻稻米加工品质和外观品质的QTL定位[J].农业生物技术学报,15(1):90-96.[Liu JF,Kui L M,Zhu Z F,Tan L B,Wang G J,Li Q W,Shu JH,Sun C Q.2007.Identification of QTLs associated with processing quality andappearance quality of common wild rice(Oryza rufipogon Griff.)[J].Journal of Agricultural Biotechnology,15(1):90-96.]
梅德勇,朱玉君,樊叶杨.2012.籼稻稻米碾磨与外观品质性状的QTL定位[J].遗传,34(12):1591-1598.[Mei D Y,Zhu Y J,Fan Y Y.2012.Mapping QTL for rice milling and appearance quality traits in indica rice[J].Hereditas,34(12):1591-1598.]
梅捍卫,罗利军,郭龙彪,王一平,余新桥,应存山,黎志康.2002.水稻加工品质数量性状基因座(QTIs)分子定位研究[J].遗传学报,29(9):791-797.[Mei H W,Luo L J,Guo L B,Wang Y P,Yu X Q,Ying C S,Li Z K.2002.Molecular mapping of QTLs for rice milling yield traits[J].Acta Genetica Sinica,29(9):791-797.]
穆平,郭咏梅,刘家富,卢义宣,李自超.2007.稻米外观和碾磨品质QTL定位及其与土壤水分环境互作分析[J].农业生物技术学报,15(4):654-660.[Mu P,Guo Y M,Liu JF,Lu Y X,Li Z C.2007.QTL mapping and QTL environments interactions of grain milling and appearance quality traits in rice under upland and lowland environments[J].Journal of Agricultural Biotechnology,15(4):654-660.]
彭强,张大双,吴健强,张玉珊,王际凤,黄培英,朱速松.2016.水稻粒形性状QTL定位分析[J].西南农业学报,29(8):1766-1770.[Peng Q,Zhang D S,Wu J Q,Zhang Y S,Wang J F,Huang P Y,Zhu S S.2016.QTL analysis of grain shape trait of rice[J].Southwest China Journal of Agricultural Sciences,29(8):1766-1770.]
邵高能,唐绍清,焦桂爱,罗炬,唐傲,胡培松.2009.稻米蒸煮品质性状的QTL定位[J].中国水稻科学,23(1):94-98.[Shao G N,Tang S Q,Jiao G A,Luo J,Tang A,Hu P S.2009.Mapping of QTL for cooking quality traits of rice[J].Chinese Journal Rice Science,23(1):94-98.]
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