大肠杆菌一步水热法制备氮掺杂碳量子点及其在铁离子检测中应用
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摘要
以大肠杆菌沉淀物为前驱体,采用水热法(180℃)一步合成水溶性好、pH稳定性和抗盐性突出的氮掺杂碳量子点(N-CQDs)。通过透射电子显微镜、动态光散射和傅立叶红外光谱对N-CQDs进行了表征,N-CQDs呈圆球状,粒径均一,大小4.1 nm,分散良好,表面含大量亲水基团;采用紫外-可见吸收光谱和荧光光谱考察其光学性能,结果表明, Fe~(3+)离子可对N-CQDs选择性荧光猝灭,在0.58 nmol/L～100μmol/L浓度范围内,荧光猝灭程度与Fe~(3+)浓度呈良好的线性关系,检出限为0.58 nmol/L,回收率为93.9%～108.7%;同时考察了pH值、含盐量、金属离子共存对Fe~(3+)测定的干扰,结果表明,本方法适用于含盐量较高、pH值变化范围较大、共存金属离子多样的近海海水中Fe~(3+)的选择性荧光检测。
Nitrogen-doped carbon quantum dots(N-CQDs) with good water solubility, pH stability and salt tolerance were synthesized by hydrothermal method(180℃) with Escherichia coli as precursor. The structure of N-CQD was characterized by transmission electron microscopy, dynamic light scattering, and Fourier transform infrared spectroscopy. The results showed that the particle size was uniform with a diameter of 4.1 nm, the dispersibility was good, and the surface contained a large amount of hydrophilic groups. UV-visible absorption and fluorescence spectra were used to study optical properties, and it was found that Fe~(3+) could selectively quench the N-CQDs fluorescence. In the concentration range of 0.58 nmol/L-100 μmol/L, the quenching degree had a good linear relationship with the concentration of Fe~(3+) ion. The detection limit was 0.58 nmol/L, and the recoveries were 93.9%-108.7%. At the same time, the interference of pH, salt content and coexistence metal ion on Fe~(3+) determination was studied. The results showed that the method was especially suitable for selective fluorescent detection of Fe~(3+) in offshore seawater with high salt content, large range of pH and diverse metal ions.
Nitrogen-doped carbon quantum dots(N-CQDs) with good water solubility, pH stability and salt tolerance were synthesized by hydrothermal method(180℃) with Escherichia coli as precursor. The structure of N-CQD was characterized by transmission electron microscopy, dynamic light scattering, and Fourier transform infrared spectroscopy. The results showed that the particle size was uniform with a diameter of 4.1 nm, the dispersibility was good, and the surface contained a large amount of hydrophilic groups. UV-visible absorption and fluorescence spectra were used to study optical properties, and it was found that Fe~(3+) could selectively quench the N-CQDs fluorescence. In the concentration range of 0.58 nmol/L-100 μmol/L, the quenching degree had a good linear relationship with the concentration of Fe~(3+) ion. The detection limit was 0.58 nmol/L, and the recoveries were 93.9%-108.7%. At the same time, the interference of pH, salt content and coexistence metal ion on Fe~(3+) determination was studied. The results showed that the method was especially suitable for selective fluorescent detection of Fe~(3+) in offshore seawater with high salt content, large range of pH and diverse metal ions.
引文
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41 Jia X F,Li J,Wang E.Nanoscale,2012,4(18):5572-5575
42 Liu W,Diao H P,Chang H H,Wang H J,Li T T,Wei W L.Sens.Actuators B,2017,241:190-198
43 Meng A L,Xu Q H,Zhao K,Li Z J,Liang J,Li Q D.Sens.Actuators B,2018,255:657-665
2 Krishna A S,Radhakumary C,Antony M,Sreenivasan K.J.Mater.Chem.B,2014,2(48):8626-8632
3 Zhang Y,Chan KF,Wang B,Chiu P W Y,Zhang L.Sens.Actuators B,2018,271:128-136
4 Wang R X,Wang X F,Sun Y M.Sens.Actuators B,2017,241:73-79
5 Atchudan R,Edison T N J I,Aseer K R,Perumal S,Karthik N,Lee Y R.Biosens.Bioelectron.,2018,99:303-311
6 Li L S,Jiao X Y,Zhang Y,Cheng C,Huang K,Xu L.Sens.Actuators B,2018,268:84-92
7 Bu D,Zhuang H S,Yang G X,Ping X X.Sens.Actuators B,2014,195:540-548
8 Niu J J,Gao H.J.Lumin.,2014,149:159-162
9 Yu C M,Li X Z,Zeng F,Zeng F Y,Wu S Z.Chem.Commun.,2013,49(4):403-405
10 Hsu P C,Chen P C,Ou C M,Chang H Y,Chang H T.J.Mater.Chem.B,2013,1(13):1774-1781
11 Tao H Q,Yang K,Ma Z,Wan J M,Zhang Y J,Kang Z H,Liu Z.Small,2012,8(2):281-290
12 Yang K,Gong H,Shi X Z,Wan J M,Zhang Y J,Liu Z.Biomaterials,2013,34(11):2787-2795
13 Guo Y M,Cao F P,Li Y B.Sens.Actuators B,2018,255:1105-1111
14 Li H T,Liu R H,Kong W Q,Liu J ,Liu Y,Zhou L,Zhang X,Lee S T,Kang Z H.Nanoscale,2014,6(2):867-873
15 Liu Y,Wu P Y.ACS Appl.Mater.Interfaces,2013,5(8):3362-3369
16 Tang D,Liu J,Wu X Y,Liu R H,Han X,Han Y Z,Huang H,Liu Y,Kang Z H.ACS Appl.Mater.Interfaces,2014,6(10):7918-7925
17 Furtado C A,Kim U J,Gutierrez H R,Pan L,Dickey E C,Eklund P C.J.Am.Chem.Soc.,2004,126(19):6095-6105
18 Sun Y P,Zhou B,Lin Y,Wang W,Fernando K A S,Pathak P,Meziani M J,Harruff B A,Wang X,Wang H,Luo P J G,Yang H,Kose M E,Chen B L,Veca L M,Xie S Y.J.Am.Chem.Soc.,2006,128(24):7756-7757
19 Zhou J G,Booker C,Li R Y,Zhou X T,Sham T K,Sun X L,Ding Z F.J.Am.Chem.Soc.,2007,129(4):744-745
20 Liu H P,Ye T,Mao C D.Angew.Chem.Int.Edit.,2007,119(34):6593-6595
21 ZOU Xiao-Bo,SHI Yong-Qiang,ZHENG Yue,SHI Ji-Yong,HU Xue-Tao,JIANG Cai-Ping,HUANG Xiao-Wei,XU Yi-Wei.Chinese J.Anal.Chem.,2018,46(6):960-968邹小波,史永强,郑悦,石吉勇,胡雪桃,蒋彩萍,黄晓玮,徐艺伟.分析化学,2018,46(6):960-968
22 Zhu H,Wang X L,Li Y L,Wang Z J,Yang F,Yang X R.Chem.Commun.,2009,(34):5118-5120
23 GUO Zhen-Zhen,TANG Yu-Guo,MENG Fan-Yu,LI Li,YANG Da-Wei.Chin.Opt.,2018,11(3):431-443郭振振,唐玉国,孟凡渝,李力,杨大威.中国光学,2018,11(3):431-443
24 Ma Q,Song J P,Wang S Z,Yang J,Guo Y,Dong C.Appl.Surf.Sci.,2016,389:995-1002
25 WANG Wen-Xiong.Trace Metal Ecotoxicology and Biogeochemistry.Beijing:Science Press,2011:1-321王文雄.微量金属生态毒理学和生物地球化学,科学出版社,2011:1-321
26 Morel,M.F.M.Principles of Aquatic Chemistry,New York:John Wiley & Sons,1983:300-309
27 Li W,Simmons P,Shrader D,Herrman T J,Dai S Y.Talanta,2013,112:43-48
28 Gao X H,Lu Y Z,He S J,Li X K,Chen W.Anal.Chim.Acta,2015,879:118-125
29 Stauffer M T,Hunter L J,Troncone S K.Spectrosc.Lett.,2007,40(3):429-437
30 Lin F M,Li C C,Dong L G,Fu D,Chen Z.Nanoscale,2017,9(26):9056-9064
31 ZHANG Hui-Jia,SHI Jing,JIN Xiao-Yong,PENG Juan.Journal of Instrumental Analysis,2018,37(6):724-728张慧佳,石静,晋晓勇,彭娟.分析测试学报,2018,37(6):724-728
32 TANG Dan-Dan,ZHANG Jin-Yi,HOU Xian-Deng,WU Peng.Chinese J.Anal.Chem.,2017,45(12):1909-1914唐丹丹,张金懿,侯贤灯,吴鹏.分析化学,2017,45(12):1909-1914
33 Yu,J,Xu C X,Tian,Z S,Lin Y,Shi Z L.New J.Chem.,2016,40(3):2083-2088
34 Wang R X,Wang X F,Sun Y M.Sens.Actuators B,2017,241:73-79
35 Zhang H J,Chen Y L,Liang M J,Xu L F,Qi S D,Chen H L,Chen X G.Anal.Chem.,2014,86(19):9846-9852
36 Wang F X,Hao Q L,Zhang Y H,Xu Y J,Lei W.Microchim.Acta,2016,183(1):273-279
37 Qu K G,Wang J S,Ren J S,Qu X G.Chem.Eur.J,2013,19(22):7243-7249
38 Feng X,Jiang Y Q,Zhao J P,Miao M,Cao S M,Fang J H,Shi L Y.RSC Adv.,2015,5(40):31250-31254
39 Alam A M,Park B Y,Ghouri Z K,Park M,Kim H Y.Green Chem.,2015,17(7):3791-3797
40 XU Yue,TANG Chun-Jing,HUANG Hong,SUN Chao-Qun,ZHANG Ya-Kun,YE Qun-Feng,WANG Ai-Jun.Chinese J.Anal.Chem.,2014,42(9):1252-1258胥月,汤纯静,黄宏,孙超群,张亚鲲,叶群峰,王爱军.分析化学,2014,42(9):1252-1258
41 Jia X F,Li J,Wang E.Nanoscale,2012,4(18):5572-5575
42 Liu W,Diao H P,Chang H H,Wang H J,Li T T,Wei W L.Sens.Actuators B,2017,241:190-198
43 Meng A L,Xu Q H,Zhao K,Li Z J,Liang J,Li Q D.Sens.Actuators B,2018,255:657-665