四倍体杂交冰草SRAP和SSR分子标记遗传连锁图谱构建
|
摘要
为了给后期冰草抗旱耐寒基因筛选及QTL图位克隆搭建平台,并为产量及相关性状QTL定位和分子标记辅助育种奠定基础,以航道冰草和蒙古冰草为亲本,杂交加倍后随机选取180个F2分离单株为作图群体,利用SRAP和SSR分子标记进行四倍体杂交冰草遗传连锁图谱的构建。结果表明,构建的图谱包含475个标记(240个SRAP标记和235个SSR标记),分布于14个连锁群,图谱全长为1 592.7cM,标记间平均间距为3.35cM,各连锁群长度范围为67.6~145.2cM。该图谱密度较高、标记位点分布均匀且连锁群更加饱满。
Construction of the genetic linkage map of tetraploid hybrid crested wheatgrass will set up a platform for the screening of drought resistant gene and the map-based cloning of QTL.And it can build the foundation for QTL and molecular marker assisted breeding of yield and related traits.In the present research,using 180 randomly selected F2 individuals of tetraploid hybrid crested wheatgrass from a cross of A.mongolicum Keng×A.cristatum cv.Fairway as mapping population,we constructed a genetic linkage map of tetraploid hybrid crested wheatgrass based on SRAP and SSR molecular markers.The genetic map contains 475 molecular markers(240 SRAP markers and 235 SSR markers),distributed in 14 linkage groups.The total length of the map was 1592.7 cM,with an average interval of 3.35 cM,and the length of each linkage group ranged from 67.6 cM to 145.2 cM.It is a genetic linkage map based on SRAP and SSR markers,with a relatively high map density,evenly distribution of marker loci,and more abundant markers on each linkage group.
Construction of the genetic linkage map of tetraploid hybrid crested wheatgrass will set up a platform for the screening of drought resistant gene and the map-based cloning of QTL.And it can build the foundation for QTL and molecular marker assisted breeding of yield and related traits.In the present research,using 180 randomly selected F2 individuals of tetraploid hybrid crested wheatgrass from a cross of A.mongolicum Keng×A.cristatum cv.Fairway as mapping population,we constructed a genetic linkage map of tetraploid hybrid crested wheatgrass based on SRAP and SSR molecular markers.The genetic map contains 475 molecular markers(240 SRAP markers and 235 SSR markers),distributed in 14 linkage groups.The total length of the map was 1592.7 cM,with an average interval of 3.35 cM,and the length of each linkage group ranged from 67.6 cM to 145.2 cM.It is a genetic linkage map based on SRAP and SSR markers,with a relatively high map density,evenly distribution of marker loci,and more abundant markers on each linkage group.
引文
[1]王方,袁庆华.冰草ISSR-PCR反应体系的建立与优化[J].草地学报,2012,17(3):354.WANG F,YUAN Q H.Establishment and optimization of ISSR reaction system for Agropyron cristatum(L.)Gaertn[J].Journal of Grass-land,2012,17(3):354.
[2]云锦凤,李瑞芬.冰草的远缘杂交及杂种分析[J].草地学报,1997,5(4):221.YUN J F,LI R F.Analysis of the distant hybridization and hybrids of Agropyron[J].Journal of Grass-land,1997,5(4):221.
[3]于卓,张志成,马艳红,等.四倍体杂交冰草新品系植株形态及生长特性分析[J].草业科学,2013,30(11):1762.YU Z,ZHANG Z C,MA Y H,et al.Analysis on morphology and growth of new lines of tetraploid hybrid wheatgrass[J].Pratacultural Science,2013,30(11):1762.
[4]马艳红,于肖夏,于卓,等.四倍体杂交冰草新品系的细胞学鉴定及SSR分析[J].麦类作物学报,2014,34(2):187.MA Y H,YU X X,YU Z,et al.Cytological identification and SSR analysis of new strains of tetraploidy hybrid wheatgrass[J].Journal of Triticeae Crops,2014,34(2):187.
[5]于卓,马艳红,李小雷,等.蒙古冰草与航道冰草正、反交F1及其染色体加倍植株AFLP分析[J].中国草地学报,2009,31(6):14.YU Z,MA Y H,LI X L,et al.AFLP analysis on reciprocal hybrids F1between Agropyron mongolicum and A.cristatum cv.fairway and its chromosome doubling plants[J].Chinese Journal of Grass-land,2009,31(6):14.
[6]解新明,云锦凤,尹俊,等.蒙古冰草遗传多样性的RAPD分析[J].西北植物学报,2002,22(1):56.JIE X M,YUN J F,YIN J,et al.RAPD analysis of genetic diversity of Agropyron mongolicum[J].Acta Botanica BorealiOccidentalia Sinica,2002,22(1):56.
[7]李媛媛,沈金雄,王同华,等.利用SRAP、SSR和AFLP标记构建甘蓝型油菜遗传连锁图谱[J].中国农业科学,2007(6):1118.LI Y Y,SHEN J X,WANG T H,et al.Construction of a linkage map using SRAP,SSR and AFLP markers in Brassica napus L.[J].Agricultural Sciences in China,2007(6):1118.
[8]王凤涛,蔺瑞明,欧阳宏雨,等.利用SRAP标记分析河南小麦栽培品种的遗传多样性[J].植物遗传资源学报,2009,10(4):517.WANG F T,LIN R M,OUYANG H Y,et al.The application of SRAP-PCR in genetic diversity study of henan wheat cultivars[J].Journal of Plant Genetic Resources,2009,10(4):517.
[9]LI G,QUIROS C F.Sequence-related amplified polymorphism(SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theoretical and Applied Genetics,2001,103(2-3):455.
[10]武耀龙,李德芳,李建军,等.利用SRAP和SSR标记构建红麻遗传连锁图谱[J].中国麻业科学,2016,38(6):249.WU Y L,LI D F,LI J J,et al.Constructing agenetic linkage map by SRAP and SSR marker for kenaf(Hibiscus cannabinus L.)[J].Plant Fiber Sciences in China,2016,38(6):249.
[11]朱洪运,颉建明,郁继华,等.结球甘蓝AFLP、SSR、SRAP标记高密度遗传图谱构建[J].华北农学报,2013,28(3):82.ZHU H Y,XIE J M,YU J H,et al.Construction of a genetic linkage map in cabbage based on AFLP,SSR and SRAP markers[J].Acta Agriculturae Boreali-Sinica,2013,28(3):82.
[12]郭利建,王竹林,汪世娟,等.基于SRAP和SSR标记的小麦品质相关性状的QTL定位[J].麦类作物学报,2016,36(10):1275.GUO L J,WANG Z L,WANG S J,et al.QTL mapping of wheat grain quality traits based on SRAP and SSR marker[J].Journal of Triticeae Crops,2016,36(10):1275.
[13]姜志艳,于肖夏,于卓,等.四倍体杂交冰草AFLP遗传连锁图谱的构建[J].麦类作物学报,2015,35(4):457.JIANG Z Y,YU X X,YU Z,et al.Construction of an AFLPbased genetic linkage map of tetraploid hybrid wheatgrass[J].Journal of Triticeae Crops,2015,35(4):457.
[14]郭印山,赵玉辉,刘朝吉,等.利用多种分子标记构建龙眼高密度分子遗传图谱[J].园艺学报,2009,36(5):655.GUO Y S,ZHAO Y H,LIU C J,et al.Construction of a molecular genetic map for longan based on RAPD,ISSR,SRAP and AFLP markers[J].Acta Horticulturae Sinica,2009,36(5):655.
[15]于卓,张志成,马艳红,等.四倍体杂交冰草新品系植株形态及生长特性分析[J].草业科学,2013,30(11):1762.YU Z,ZHANG Z C,MA Y H,et al.Analysis on morphology and growth of new lines of tetraploid hybrid wheatgrass[J].Pratacultural Science,2013,30(11):1762.
[16]PEZZOTTI G,YAMADA K.Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance[J].Plant Breeding,2010,120(5):411.
[17]LIN Z,HE D,ZHANG X,et al.Linkage map construction and mapping QTL for cotton fibre quality using SRAP,SSR and RAPD[J].Plant Breeding,2010,124(2):180.
[18]石悦,于肖夏,于卓,等.高丹草AFLP分子遗传连锁图谱的构建[J].西北农林科技大学学报(自然科学版),2017,45(10):7.SHI Y,YU X X,YU Z,et al.Construction of AFLP molecular genetic linkage map of sorghum-sudangrass[J].Journal of Northwest A&F University(Natural Science Edition),2017,45(10):7.
[19]TUINSTR M R,GROTE E M,GOLDSBROUGH P B,et al.Identification of quantitative trait loci associated with preflowering drought tolerance in sorghum[J].Crop Science,1996,36(5):1337.
[20]陈晖,陈美霞,陶爱芬,等.长果种黄麻SRAP标记遗传连锁图谱的构建及3个质量性状基因定位[J].中国农业科学,2011,44(12):2422.CHEN H,CHEN M X,TAO A F,et al.Construction of a molecular linkage map using SRAP and mapping of three qualitative traits in Corchorus olitorius L.[J].Agricultural Sciences in China,2011,44(12):2422.
[21]CASTIGLIONI P,AJMONEMARSAN P,VAN W R,et al.AFLP markers in a molecular linkage map of maize:codominant scoring and linkage group distribution[J].Theoretical and Applied Genetics,1999,99(3-4):425.
[22]金梦阳,刘列钊,付福友,等.甘蓝型油菜SRAP、SSR、AFLP和TRAP标记遗传图谱构建[J].分子植物育种,2006,4(4):520.JIN M Y,LIU L Z,FU F Y,et al.Construction of a genetic linkage map in Brassica napus based on SRAP,SSR,AFLP and TRAP[J].Molecular Plant Breeding,2006,4(4):520.
[23]HASHIZUME T,SHIMAMOTO I,HIRAI M.Construction of a linkage map and QTL analysis of horticultural traits for watermelon[Citrullus lanatus(THUNB.)MATSUM&NAKAI]using RAPD,RFLP and ISSR markers[J].Theoretical and Applied Genetics,2003,106(5):779.
[24]LI Y C,KOROL A B,FAHIMA T,et al.Microsatellites:genomic distribution,putative functions and mutational mechanisms:a review[J].Molecular Ecology,2002,11(12):2453.
[25]FOISSET N,DELOURME R,BARRET P,et al.Molecularmapping analysis in Brassica napus using isozyme,RAPD and RFLP markers on a doubled-haploid progeny[J].Theoretical and Applied Genetics,1996,93(7):1017.
[26]KONISHI T,YANO Y,ABE K.Geographic distribution of alleles at the Ga2,locus for segregation distortion in barley[J].Theoretical and Applied Genetics,1992,85(4):419.
[27]HARUSHIMA Y,KURATA N,YANO M,et al.Detection of segregation distortions in an indica-japonica,rice cross using a high-resolution molecular map[J].Theoretical and Applied Genetics,1996,92(2):145.
[28]PATERSON A H,DAMON S,HEWITT J D,et al.Mendelian factors underlying quantitative traits in tomato:comparison across species,generations,and environments[J].Genetics,1991,127(1):181.
[29]PERFECTTI F,PASCUAL L.Segregation distortion of isozyme loci in cherimoya(Annona cherimola Mill.)[J].Theoretical and Applied Genetics,1996,93(3):440.
[30]PAWLOWSKI W P,TORBERT K A,RINES H W,et al.Irregular patterns of transgene silencing in allohexaploid oat[J].Plant Molecular Biology,1998,38(4):597.
[2]云锦凤,李瑞芬.冰草的远缘杂交及杂种分析[J].草地学报,1997,5(4):221.YUN J F,LI R F.Analysis of the distant hybridization and hybrids of Agropyron[J].Journal of Grass-land,1997,5(4):221.
[3]于卓,张志成,马艳红,等.四倍体杂交冰草新品系植株形态及生长特性分析[J].草业科学,2013,30(11):1762.YU Z,ZHANG Z C,MA Y H,et al.Analysis on morphology and growth of new lines of tetraploid hybrid wheatgrass[J].Pratacultural Science,2013,30(11):1762.
[4]马艳红,于肖夏,于卓,等.四倍体杂交冰草新品系的细胞学鉴定及SSR分析[J].麦类作物学报,2014,34(2):187.MA Y H,YU X X,YU Z,et al.Cytological identification and SSR analysis of new strains of tetraploidy hybrid wheatgrass[J].Journal of Triticeae Crops,2014,34(2):187.
[5]于卓,马艳红,李小雷,等.蒙古冰草与航道冰草正、反交F1及其染色体加倍植株AFLP分析[J].中国草地学报,2009,31(6):14.YU Z,MA Y H,LI X L,et al.AFLP analysis on reciprocal hybrids F1between Agropyron mongolicum and A.cristatum cv.fairway and its chromosome doubling plants[J].Chinese Journal of Grass-land,2009,31(6):14.
[6]解新明,云锦凤,尹俊,等.蒙古冰草遗传多样性的RAPD分析[J].西北植物学报,2002,22(1):56.JIE X M,YUN J F,YIN J,et al.RAPD analysis of genetic diversity of Agropyron mongolicum[J].Acta Botanica BorealiOccidentalia Sinica,2002,22(1):56.
[7]李媛媛,沈金雄,王同华,等.利用SRAP、SSR和AFLP标记构建甘蓝型油菜遗传连锁图谱[J].中国农业科学,2007(6):1118.LI Y Y,SHEN J X,WANG T H,et al.Construction of a linkage map using SRAP,SSR and AFLP markers in Brassica napus L.[J].Agricultural Sciences in China,2007(6):1118.
[8]王凤涛,蔺瑞明,欧阳宏雨,等.利用SRAP标记分析河南小麦栽培品种的遗传多样性[J].植物遗传资源学报,2009,10(4):517.WANG F T,LIN R M,OUYANG H Y,et al.The application of SRAP-PCR in genetic diversity study of henan wheat cultivars[J].Journal of Plant Genetic Resources,2009,10(4):517.
[9]LI G,QUIROS C F.Sequence-related amplified polymorphism(SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theoretical and Applied Genetics,2001,103(2-3):455.
[10]武耀龙,李德芳,李建军,等.利用SRAP和SSR标记构建红麻遗传连锁图谱[J].中国麻业科学,2016,38(6):249.WU Y L,LI D F,LI J J,et al.Constructing agenetic linkage map by SRAP and SSR marker for kenaf(Hibiscus cannabinus L.)[J].Plant Fiber Sciences in China,2016,38(6):249.
[11]朱洪运,颉建明,郁继华,等.结球甘蓝AFLP、SSR、SRAP标记高密度遗传图谱构建[J].华北农学报,2013,28(3):82.ZHU H Y,XIE J M,YU J H,et al.Construction of a genetic linkage map in cabbage based on AFLP,SSR and SRAP markers[J].Acta Agriculturae Boreali-Sinica,2013,28(3):82.
[12]郭利建,王竹林,汪世娟,等.基于SRAP和SSR标记的小麦品质相关性状的QTL定位[J].麦类作物学报,2016,36(10):1275.GUO L J,WANG Z L,WANG S J,et al.QTL mapping of wheat grain quality traits based on SRAP and SSR marker[J].Journal of Triticeae Crops,2016,36(10):1275.
[13]姜志艳,于肖夏,于卓,等.四倍体杂交冰草AFLP遗传连锁图谱的构建[J].麦类作物学报,2015,35(4):457.JIANG Z Y,YU X X,YU Z,et al.Construction of an AFLPbased genetic linkage map of tetraploid hybrid wheatgrass[J].Journal of Triticeae Crops,2015,35(4):457.
[14]郭印山,赵玉辉,刘朝吉,等.利用多种分子标记构建龙眼高密度分子遗传图谱[J].园艺学报,2009,36(5):655.GUO Y S,ZHAO Y H,LIU C J,et al.Construction of a molecular genetic map for longan based on RAPD,ISSR,SRAP and AFLP markers[J].Acta Horticulturae Sinica,2009,36(5):655.
[15]于卓,张志成,马艳红,等.四倍体杂交冰草新品系植株形态及生长特性分析[J].草业科学,2013,30(11):1762.YU Z,ZHANG Z C,MA Y H,et al.Analysis on morphology and growth of new lines of tetraploid hybrid wheatgrass[J].Pratacultural Science,2013,30(11):1762.
[16]PEZZOTTI G,YAMADA K.Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance[J].Plant Breeding,2010,120(5):411.
[17]LIN Z,HE D,ZHANG X,et al.Linkage map construction and mapping QTL for cotton fibre quality using SRAP,SSR and RAPD[J].Plant Breeding,2010,124(2):180.
[18]石悦,于肖夏,于卓,等.高丹草AFLP分子遗传连锁图谱的构建[J].西北农林科技大学学报(自然科学版),2017,45(10):7.SHI Y,YU X X,YU Z,et al.Construction of AFLP molecular genetic linkage map of sorghum-sudangrass[J].Journal of Northwest A&F University(Natural Science Edition),2017,45(10):7.
[19]TUINSTR M R,GROTE E M,GOLDSBROUGH P B,et al.Identification of quantitative trait loci associated with preflowering drought tolerance in sorghum[J].Crop Science,1996,36(5):1337.
[20]陈晖,陈美霞,陶爱芬,等.长果种黄麻SRAP标记遗传连锁图谱的构建及3个质量性状基因定位[J].中国农业科学,2011,44(12):2422.CHEN H,CHEN M X,TAO A F,et al.Construction of a molecular linkage map using SRAP and mapping of three qualitative traits in Corchorus olitorius L.[J].Agricultural Sciences in China,2011,44(12):2422.
[21]CASTIGLIONI P,AJMONEMARSAN P,VAN W R,et al.AFLP markers in a molecular linkage map of maize:codominant scoring and linkage group distribution[J].Theoretical and Applied Genetics,1999,99(3-4):425.
[22]金梦阳,刘列钊,付福友,等.甘蓝型油菜SRAP、SSR、AFLP和TRAP标记遗传图谱构建[J].分子植物育种,2006,4(4):520.JIN M Y,LIU L Z,FU F Y,et al.Construction of a genetic linkage map in Brassica napus based on SRAP,SSR,AFLP and TRAP[J].Molecular Plant Breeding,2006,4(4):520.
[23]HASHIZUME T,SHIMAMOTO I,HIRAI M.Construction of a linkage map and QTL analysis of horticultural traits for watermelon[Citrullus lanatus(THUNB.)MATSUM&NAKAI]using RAPD,RFLP and ISSR markers[J].Theoretical and Applied Genetics,2003,106(5):779.
[24]LI Y C,KOROL A B,FAHIMA T,et al.Microsatellites:genomic distribution,putative functions and mutational mechanisms:a review[J].Molecular Ecology,2002,11(12):2453.
[25]FOISSET N,DELOURME R,BARRET P,et al.Molecularmapping analysis in Brassica napus using isozyme,RAPD and RFLP markers on a doubled-haploid progeny[J].Theoretical and Applied Genetics,1996,93(7):1017.
[26]KONISHI T,YANO Y,ABE K.Geographic distribution of alleles at the Ga2,locus for segregation distortion in barley[J].Theoretical and Applied Genetics,1992,85(4):419.
[27]HARUSHIMA Y,KURATA N,YANO M,et al.Detection of segregation distortions in an indica-japonica,rice cross using a high-resolution molecular map[J].Theoretical and Applied Genetics,1996,92(2):145.
[28]PATERSON A H,DAMON S,HEWITT J D,et al.Mendelian factors underlying quantitative traits in tomato:comparison across species,generations,and environments[J].Genetics,1991,127(1):181.
[29]PERFECTTI F,PASCUAL L.Segregation distortion of isozyme loci in cherimoya(Annona cherimola Mill.)[J].Theoretical and Applied Genetics,1996,93(3):440.
[30]PAWLOWSKI W P,TORBERT K A,RINES H W,et al.Irregular patterns of transgene silencing in allohexaploid oat[J].Plant Molecular Biology,1998,38(4):597.