RNAi在昆虫基因功能研究中的应用进展
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摘要
RNA干扰(RNA interference,RNAi)是由小分子dsRNA引起目的基因mRNA序列特异性降解,导致靶标基因沉默或表达量下调的现象。RNAi在昆虫重要生理酶相关基因功能的研究中起着至关重要的作用。本文主要对RNAi不同方法的应用、各方法的优缺点及影响RNAi效率的因素进行了综述,总结了RNAi不同方法对昆虫功能基因沉默效果的差异,及在昆虫基因功能研究中的应用进展。
RNA interference( RNAi) is a phenomenon in which small-molecule dsRNA causes sequence-specific degradation of the target gene mRNA,resulting in gene silencing or down-regulated expression of target gene.RNAi plays a crucial role in studying the functions of relative genes of important physiological enzymes.This paper mainly reviewsdifferent methods of RNAi,their advantages and disadvantages,and the factors affecting RNAi efficiency.The differences of silencing effects of different RNAi methods on insect functional genes were compared,and the progress in insect gene function were summarized.
RNA interference( RNAi) is a phenomenon in which small-molecule dsRNA causes sequence-specific degradation of the target gene mRNA,resulting in gene silencing or down-regulated expression of target gene.RNAi plays a crucial role in studying the functions of relative genes of important physiological enzymes.This paper mainly reviewsdifferent methods of RNAi,their advantages and disadvantages,and the factors affecting RNAi efficiency.The differences of silencing effects of different RNAi methods on insect functional genes were compared,and the progress in insect gene function were summarized.
引文
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[13]陈静,张道伟,钱正敏.白背飞虱几丁质合成酶1基因的结构及特性研究[J].生物技术通报,2018(1):195-201.
[14]公玲玲,张丹丹,郑曰英,等.棉铃虫ABC转运蛋白ABCG1的基因克隆、亚细胞定位及其与Cry1Ac毒力的关系[J].昆虫学报,2017,60(3):297-308.
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[18]罗静.中黑盲蝽信息素合成相关基因的筛选及功能分析[D].武汉:华中农业大学,2017.
[19]吴艳艳,周婷. RNA干扰在蜜蜂病害防治中的应用[J].中国蜂业,2013(16):58.
[20]崔杰,周金林.蜱RNA干扰的不同方法与应用[J].寄生虫与医学昆虫学报,2015(1):65-71.
[21]敖已倩云,申光茂,王梦瑶,等.朱砂叶螨β-COP和Sro基因鉴定及其沉默致死效果[J].中国农业科学,2017,50(18):3529-3539.
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[27] Konopova B,Jindra M. Broad-Complex acts downstream of Met in juvenile hormone signaling to coordinate primitive holometabolan metamorphosis[J].Development,2008,135(3):559-568.
[28] Badillo-Vargas I E,Rotenberg D,Schneweis B A,et al. RNA interference tools for the western flower thrips,Frankliniella occidentalis[J].Journal of Insect Physiology,2015(76):36-46.
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