贵州民族药苦皮藤药材质量标准研究
|
摘要
目的:为贵州民族药材苦皮藤建立一个质量控制方法。方法:采用薄层色谱法对苦皮藤药材中儿茶素进行鉴别;采用高效液相色谱法对药材中儿茶素的含量进行测定,色谱条件:JADE-PAK ODS-AQ色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.5%冰醋酸溶液(7.8∶92.2)为流动相,检测波长280 nm,柱温30℃,流速1.0 mL/min。结果:薄层色谱法鉴别苦皮藤药材的特征斑点清晰,分离度良好;儿茶素在0.12μg～1.56μg范围内线性关系良好,线性回归方程为y=369.51x-1.7732(r=0.9999),精密度及加样回收试验结果符合质量标准研究的要求。结论:该法简便易用,专属性好、结果准确,可用于苦皮藤药材的质量控制。
In order to establish a quality control method for Guizhou ethnic medicine Celastrus angulatus Maxim,we adopted thin layer chromatography to identify the catechin in Celastrus angulatus,and high performance liquid chromatography to determine the content of catechin.The HPLC parameters were as follows:JADE-PAK ODS-AQ column(4.6 mm×250 mm,5 μm),the mobile phase was acetonitrile-0.5% glacial acetic acid solution(7.8∶92.2),the detection wavelength was 280 nm,the column temperature was 30 ℃,and the flow rate was 1.0 mL/min.TLC results showed clear characteristic spots and good separation.The linear relationship of catechin whtin the range of 0.12 μg to 1.56 μg was good,and the linear regression equation was y=369.51 x-1.7732(r=0.9999).Precision test and recovery test results met the requirements of quality control standards.This method is simple and easy to operate,has good specificity and accurate results,and can be used for quality control of Celastrus angulatus Maxim.
In order to establish a quality control method for Guizhou ethnic medicine Celastrus angulatus Maxim,we adopted thin layer chromatography to identify the catechin in Celastrus angulatus,and high performance liquid chromatography to determine the content of catechin.The HPLC parameters were as follows:JADE-PAK ODS-AQ column(4.6 mm×250 mm,5 μm),the mobile phase was acetonitrile-0.5% glacial acetic acid solution(7.8∶92.2),the detection wavelength was 280 nm,the column temperature was 30 ℃,and the flow rate was 1.0 mL/min.TLC results showed clear characteristic spots and good separation.The linear relationship of catechin whtin the range of 0.12 μg to 1.56 μg was good,and the linear regression equation was y=369.51 x-1.7732(r=0.9999).Precision test and recovery test results met the requirements of quality control standards.This method is simple and easy to operate,has good specificity and accurate results,and can be used for quality control of Celastrus angulatus Maxim.
引文
[1]贵州省食品药品监督管理局.贵州省中药材、民族药材质量标准[M].贵阳:贵州科技出版社,2003:85.
[2]陈佩东,梁敬钰.苦皮藤根皮的化学成分研究[J].海峡药学,2002,14(4):33-35.CHEN P D,LIANG J Y.Study on the root barks of Celastrus angulatus[J].Strait Pharmaceutical Journal,2002,14(4):33-35.
[3]刘绣华,马同森,王敏,等.苦皮藤中酚性成分抗羟自由基活性研究[J].天然产物研究与开发,1999,11(5):11-14.LIU X H,MA T S,WANG M,et al.The scavenging effect of phenolic compounds from C.angulatus on hydroxyl free radicals[J].Natural Product Research and Development,1999,11(5):11-14.
[4]WANG D X,LIU P,CHEN Y H,et al.Stimulating effect of catechin,an active component of Spatholobus suberectus Dunn,on bioactivity of hematopoietic growth factor[J].Chinese Medical Journal,2008,121(8):752-755.
[5]涂永强,宋庆宝,武小莉,等.南蛇藤倍半萜成分研究[J].化学学报,1993,51:404-408.TU Y Q,SONG Q B,WU X L,et al.Study on sesquiterpene constituents from Celastrus angulatus[J].Journal of Chemistry,1993,51(4):404-408.
[6]王国亮,南蓬,南玉生,等.苦皮藤酯2、3、4的结构鉴定[J].植物学报,1992,31(10):777-780.WANG G L,NAN P,NAN Y S,et al.Structural identification of kupitengester 2,3 and 4[J].Acta Botanica Sinica,1992,31(10):777-780.
[7]TU Y Q,HUANG G S,MA Y X.Polyesters of a sesquiterpene pentaol from Celastrus angulatus[J].Phytochemistry,1993,32(2):458-459.
[8]赵天增,尹卫平,秦海林,等.苦皮藤中一个倍半萜醇酯新活性化合物的结构[J].中草药,1999,30(4):241-244.ZHAO T Z,YIN W P,QIN H L,et al.A novel bioactive sesquiterpene polyol ester from angled bittersweet leaves(Celastrus angulatus)[J].Chinese Traditional and Herbal Drugs,1999,30(4):241-244.
[9]刘吉开,贾忠建,吴大刚,等.南蛇藤植物中四个新生物碱的化学结构[J].科学通报,1988,33(20):1586-1588.
[10]吴文君,胡兆龙,刘惠霞,等.苦皮藤主要杀虫有效成分的杀虫作用机理及其应用[J].昆虫学报,2005,48(5):770-777.WU W J,HU Z L,LIU H X,et al. Insecticidal mechanisms of the major active components from the Chinese bittersweet,Celastrus angulatus and their application[J]. Acta Entomologica Sinica,2005,48(5):770-777.
[11]华琼,侯勇谋,高华,等.茶多酚片改善原发性肾病综合征患者血液流变学的临床观察[J].河南中医药学刊,1997,12(5):47-48.
[2]陈佩东,梁敬钰.苦皮藤根皮的化学成分研究[J].海峡药学,2002,14(4):33-35.CHEN P D,LIANG J Y.Study on the root barks of Celastrus angulatus[J].Strait Pharmaceutical Journal,2002,14(4):33-35.
[3]刘绣华,马同森,王敏,等.苦皮藤中酚性成分抗羟自由基活性研究[J].天然产物研究与开发,1999,11(5):11-14.LIU X H,MA T S,WANG M,et al.The scavenging effect of phenolic compounds from C.angulatus on hydroxyl free radicals[J].Natural Product Research and Development,1999,11(5):11-14.
[4]WANG D X,LIU P,CHEN Y H,et al.Stimulating effect of catechin,an active component of Spatholobus suberectus Dunn,on bioactivity of hematopoietic growth factor[J].Chinese Medical Journal,2008,121(8):752-755.
[5]涂永强,宋庆宝,武小莉,等.南蛇藤倍半萜成分研究[J].化学学报,1993,51:404-408.TU Y Q,SONG Q B,WU X L,et al.Study on sesquiterpene constituents from Celastrus angulatus[J].Journal of Chemistry,1993,51(4):404-408.
[6]王国亮,南蓬,南玉生,等.苦皮藤酯2、3、4的结构鉴定[J].植物学报,1992,31(10):777-780.WANG G L,NAN P,NAN Y S,et al.Structural identification of kupitengester 2,3 and 4[J].Acta Botanica Sinica,1992,31(10):777-780.
[7]TU Y Q,HUANG G S,MA Y X.Polyesters of a sesquiterpene pentaol from Celastrus angulatus[J].Phytochemistry,1993,32(2):458-459.
[8]赵天增,尹卫平,秦海林,等.苦皮藤中一个倍半萜醇酯新活性化合物的结构[J].中草药,1999,30(4):241-244.ZHAO T Z,YIN W P,QIN H L,et al.A novel bioactive sesquiterpene polyol ester from angled bittersweet leaves(Celastrus angulatus)[J].Chinese Traditional and Herbal Drugs,1999,30(4):241-244.
[9]刘吉开,贾忠建,吴大刚,等.南蛇藤植物中四个新生物碱的化学结构[J].科学通报,1988,33(20):1586-1588.
[10]吴文君,胡兆龙,刘惠霞,等.苦皮藤主要杀虫有效成分的杀虫作用机理及其应用[J].昆虫学报,2005,48(5):770-777.WU W J,HU Z L,LIU H X,et al. Insecticidal mechanisms of the major active components from the Chinese bittersweet,Celastrus angulatus and their application[J]. Acta Entomologica Sinica,2005,48(5):770-777.
[11]华琼,侯勇谋,高华,等.茶多酚片改善原发性肾病综合征患者血液流变学的临床观察[J].河南中医药学刊,1997,12(5):47-48.