化学杀雄剂SQ-1对谷子幼穗生理生化指标的影响
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摘要
以豫谷1号为实验材料,研究化学杀雄剂杀雄嗪酸(2-(4-氯苯基)-3-乙基-2,5-二氢-5-氧哒嗪-4-羧酸,SQ-1)对谷子幼穗5个典型生理生化指标——丙二醛(MDA)含量、电导率(EC)、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性的影响.研究结果表明:使用SQ-1处理后的幼穗EC第1天达到峰值,随后呈现逐渐下降的趋势;MDA含量波动幅度较小,呈现先上升后降低的趋势,第5天达到最大值;SOD活性呈现先降低后上升再降低的趋势,第4天达到最高点;POD活性呈现先降低后上升的趋势,第7天达到最大值;CAT活性第5天有小幅度下降,总体呈现逐渐上升的趋势.本研究为后续探究SQ-1导致谷子不育的分子机理提供数据支持,也为今后谷子杂交育种奠定理论基础.
The physiological-biochemical indexes of the panicle of foxtail millet were studied under the male-gametocide SQ-1.Yugu 1 as a main foxtail millet cultivar was used in this study.The five physiological indexes of the young panicle were determined, including malondialdehyde(MDA) content, electrical conductivity(EC), activity of peroxidase(POD), superoxide dismutase(SOD) activity and catalase(CAT) activity.The results indicated that the young panicle conductivity was significantly increased under SQ-1 and gradually declined in the first day.The fluctuation of malondialdehyde was small and the results showed the trend was first rised and then decreased, however, the maximum was observed at the fifth day.SOD activity displayed variation pattern and reached the highest point at the fourth day.POD activity was decreased and then increased and reached the maximum at the seventh day.CAT activity at the fifth day was decreased slightly and the whole trend showed a gradual increase.This study provided data support for subsequent exploring the molecular mechanism of foxtail millet infertility under SQ-1,and would lay a theoretical basis for foxtail millet hybrid breeding in the future.
The physiological-biochemical indexes of the panicle of foxtail millet were studied under the male-gametocide SQ-1.Yugu 1 as a main foxtail millet cultivar was used in this study.The five physiological indexes of the young panicle were determined, including malondialdehyde(MDA) content, electrical conductivity(EC), activity of peroxidase(POD), superoxide dismutase(SOD) activity and catalase(CAT) activity.The results indicated that the young panicle conductivity was significantly increased under SQ-1 and gradually declined in the first day.The fluctuation of malondialdehyde was small and the results showed the trend was first rised and then decreased, however, the maximum was observed at the fifth day.SOD activity displayed variation pattern and reached the highest point at the fourth day.POD activity was decreased and then increased and reached the maximum at the seventh day.CAT activity at the fifth day was decreased slightly and the whole trend showed a gradual increase.This study provided data support for subsequent exploring the molecular mechanism of foxtail millet infertility under SQ-1,and would lay a theoretical basis for foxtail millet hybrid breeding in the future.
引文
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[22] 叶景秀,张改生,王书平,等.杀雄剂SQ-1诱导小麦雄性不育花粉粒差异蛋白质组学研究[J].中国生物化学与分子生物学报,2009,25(10):949-957.
[23] 叶亚新,栗冠珍.除草剂对萝卜幼苗逆境生理指标的影响[J].江苏农业科学,2012,40(10):146-149.DOI:10.3969/j.issn.1002-1302.2012.10.052.
[24] ZHANG H Y,GUO P Y,WANG Y G,et al.Assessment of male sterility and antioxidant enzyme activities induced by the chemical hybridization agent SQ-1 in Foxtail Millet (Setaria italica)[J].Emirates Journal of Food and Agriculture,2017,29(3):212-221.DOI:10.9755/ejfa.2016-10-1530.
[2] 王小青.中国粟作农业起源研究综述[J].黑龙江史志,2013,23(21):30-32.DOI:10.3969/j.issn.1004-020X.2013.21.020.
[3] 王凡.豫谷一号BAC文库的构建及鉴定[D].石家庄:河北师范大学,2009.
[4] 王亮明.杀雄剂SQ-1诱导谷子雄性不育研究[D].杨凌:西北农林科技大学,2011.
[5] 赵治海,崔文生,杜贵,等.谷子光(温)敏不育系821选育及其不育性与光、温关系的研究[J].中国农业科学,1996,29(5):23-31.
[6] 李志华,景小兰,穆婷婷.谷子雄性不育系利用及存在问题[J].中国种业,2016(6):11-13.DOI:10.19462/j.cnki.1671-895x.2016.06.004.
[7] 陈绥莉.化学杂交剂诱导谷子雄性不育效果初探[J].陕西农业科学,2013,59(6):27-29.DOI:10.3969/j.issn.0488-5368.2013.06.009.
[8] 崔秀辉.化学杂交剂SQ-1诱导糜子雄性不育效果研究[J].作物学报,2008,34(1):106-110.DOI:10.3321/j.issn:0496-3490.2008.01.016.
[9] 宋瑜龙,王亮明,张改生,等.杀雄剂SQ-1诱导谷子雄性不育研究[J/OL].作物学报,2011,37(9):1695-1700.
[10] 王强.化学杀雄剂对棉花杀雄机理的研究[D].重庆:西南农业大学,2001.
[11] 范宝磊,岳霞丽,郑青,等.化学杀雄剂对油菜叶片和花蕾中几种酶活性的影响[J].湖北农业科学,2008,47(4):406-408.
[12] 赵彩霞,赵艳宁,孙万仓,等.化学杀雄剂GSC和SX-1对白菜型冬油菜生理生化特性的影响[J].西北植物学报,2014,34(3):516-522.
[13] 崔建民,宋兆静,李晶,等.新型化学杀雄剂CHA1对油菜膜脂过氧化及保护酶活性的影响[J].西北农业学报,2011,20(11):66-69.DOI:10.3969/j.issn.1004-1389.2011.11.014.
[14] 姬俊华,孟超敏,何庆飞.不同老化处理时间对棉花种子生理生化的影响[J].种子,2017,36(11):14-17.DOI:10.16590/j.cnki.1001-4705.2017.11.014.
[15] 赵丽英,邓西平,山仑.活性氧清除系统对干旱胁迫的响应机制[J].西北植物学报,2005,25(2):413-418.
[16] 夏民旋,王维,袁瑞,等.超氧化物歧化酶与植物抗逆性[J].分子植物育种,2015,13(11):2633-2646.
[17] 陈鸿鹏,谭晓风.超氧化物歧化酶(SOD)研究综述[J].经济林研究,2007,25(1):59-65.
[18] 蒋选利,李振岐,康振生.过氧化物酶与植物抗病性研究进展[J].西北农林科技大学学报(自然科学版),2001,29(6):124-129.
[19] 张坤生,田荟琳.过氧化氢酶的功能及研究[J].食品科技,2007,32(1):8-11.
[20] 张凯,乔新荣.化学杀雄剂2号诱导水稻雄性不育花药的内源激素变化特性[J].江苏农业科学,2016,44(8):95-96.DOI:10.15889/j.issn.1002-1302.2016.08.025.
[21] 黄雪清,高东迎,杨安南,等.化学杀雄剂Ⅲ号诱导水稻雄性不育过程中幼穗、颖花、花药中核酸和蛋白质代谢研究[J].作物学报,2001,27(6):827-831.
[22] 叶景秀,张改生,王书平,等.杀雄剂SQ-1诱导小麦雄性不育花粉粒差异蛋白质组学研究[J].中国生物化学与分子生物学报,2009,25(10):949-957.
[23] 叶亚新,栗冠珍.除草剂对萝卜幼苗逆境生理指标的影响[J].江苏农业科学,2012,40(10):146-149.DOI:10.3969/j.issn.1002-1302.2012.10.052.
[24] ZHANG H Y,GUO P Y,WANG Y G,et al.Assessment of male sterility and antioxidant enzyme activities induced by the chemical hybridization agent SQ-1 in Foxtail Millet (Setaria italica)[J].Emirates Journal of Food and Agriculture,2017,29(3):212-221.DOI:10.9755/ejfa.2016-10-1530.