基因工程抗体在微囊藻毒素检测分析中的应用研究
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摘要
微囊藻毒素是蓝藻水华过程中产生的一类小分子多肽生物毒素,能对人和动物的肝脏造成严重损伤,属于2B类致癌物质。由于全球性工业和生活废水的大量排放以及气候变暖等现实因素,蓝藻水华现象日趋频繁和加剧,使得微囊藻毒素在水体和土壤中大量蓄积,严重威胁生态环境和农产品质量安全。基于"抗体-抗原"识别原理的免疫学检测方法是当前生物毒素快速检测研究的热点,近年抗体创制技术突飞猛进,现已从传统的多克隆抗体和单克隆抗体逐渐发展到了新型的基因工程人工抗体创制阶段。该文系统阐述了微囊藻毒素产生的内外因素及其对生态系统、人和动物的主要危害风险;全面梳理了基因工程抗体创制技术及其在微囊藻毒素检测应用研究上的最新进展;并结合作者及所在科研团队最新研究成果,深入探讨了基因工程抗体在微囊藻毒素检测应用上的发展趋势、存在的问题以及拟解决的对策。
Microcystin(MC),a type of small molecule polypeptide biotoxin produced by algal blooms,is high toxic to humans and animals,and has been classified as a potential human carcinogen(Group 2 B).Algal blooms break out more and more frequently and tempestuously due to the large discharge of worldwide industrial and domestic wastewater and the climate warming worldwide,which caused the massive accumulation of MCs in water-bodies and soil,threatening the ecological environment and affecting the quality and safety of agricultural products.The immunoassay based on "antibody-antigen" recognition principle is a hot spot research in the rapid determination of biotoxins.The antibody preparation technology is developing in leaps and bounds in the late years,and now has been developed from traditional polyclonal antibody and monoclonal antibody to novel genetic engineering artificial antibody.The internal and external factors of microcystin production,and major hazards toward ecosystem,humans and animals are expounded systematically in the present paper,and the latest research progress of novel artificial antibody preparation technologies and their applications in MC-LR determination are combed comprehensively.Finally,combined with our latest research results,the development trend,existing problems and solutions of novel artificial antibodies in MC-LR determination are further discussed.
Microcystin(MC),a type of small molecule polypeptide biotoxin produced by algal blooms,is high toxic to humans and animals,and has been classified as a potential human carcinogen(Group 2 B).Algal blooms break out more and more frequently and tempestuously due to the large discharge of worldwide industrial and domestic wastewater and the climate warming worldwide,which caused the massive accumulation of MCs in water-bodies and soil,threatening the ecological environment and affecting the quality and safety of agricultural products.The immunoassay based on "antibody-antigen" recognition principle is a hot spot research in the rapid determination of biotoxins.The antibody preparation technology is developing in leaps and bounds in the late years,and now has been developed from traditional polyclonal antibody and monoclonal antibody to novel genetic engineering artificial antibody.The internal and external factors of microcystin production,and major hazards toward ecosystem,humans and animals are expounded systematically in the present paper,and the latest research progress of novel artificial antibody preparation technologies and their applications in MC-LR determination are combed comprehensively.Finally,combined with our latest research results,the development trend,existing problems and solutions of novel artificial antibodies in MC-LR determination are further discussed.
引文
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[53] Pirez-Schirmer M,Rossotti M,Badagian N,Leizagoyen C,Brena B M,Gonzalez-Sapienza G.Anal.Chem.,2017,89(12):6800-6806.
[54] Xu C X,Zhang X,Liu X,Liu Y,Hu X,Zhong J F,Zhang C Z,Liu X J.Anal.Biochem.,2016,512:70-77.
[55] Ahmad Z A,Yeap S K,Ali A M,Ho W Y,Alitheen N B,Hamid M.Clin.Dev.Immunol.,2012,2012:1-15.
[56] Zhao A,Tohidkia M R,Siegel D L,Coukos G,Omidi Y.Crit.Rev.Biotechnol.,2016,36(2):276-289.
[57] Li M,Zhu M,Zhang C Z,Liu X J,Wan Y K.Toxins,2014,6(12):3208-3222.
[58] Zhong J F,Hu X,Zhang X,Liu Y,Xu C X,Zhang C Z,Lin M,Liu X J.Int.J.Biol.Macromol.,2018,107:920-928.
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[60] Xu C X,Liu X,Zhang C Z,Zhang X,Zhong J F,Liu Y,Hu X,Lin M,Liu X J.Anal.Biochem.,2017,518:53-59.