新西兰大白兔组织工程骨的构建及体内异位成骨效果
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摘要
目的:探讨新西兰大白兔组织工程骨股骨旁异位成骨(ectopic bone formation)的效果。方法:选取新西兰大白兔骨髓基质干细胞进行成骨诱导,与生物陶瓷构建组织工程骨,种植到新西兰大白兔股骨的骨膜旁,第8周获得异位成骨组织,检测分析收获的异位成骨材料结构。结果:第8周的异位成骨材料内有骨组织形成,成骨材料结构清晰,组织内部有新血管形成,荧光蛋白标记的标本有荧光蛋白表达。结论:组织工程骨在体内可顺利异位成骨。
Objective:To study the structure of ectopic bone formed beside the New-Zealand rabbit femoral bone.Methods:Bone marrow stroma stem cells(BMSCs)were taken from New-Zealand rabbits to built engineered bone constructs,the constructs were embedded near the rabbit femoral bone,the ectopic bone constructs were harvested at 8th week for further examination and study.Results:At 8th week,the ectopic bone had a clear bone-like structure,together with new blood supply,green fluorescent protein could be seen in samples seeded by marked cells.Conclusions:This study showed that the ectopic bone formation could be successful in vivo.
Objective:To study the structure of ectopic bone formed beside the New-Zealand rabbit femoral bone.Methods:Bone marrow stroma stem cells(BMSCs)were taken from New-Zealand rabbits to built engineered bone constructs,the constructs were embedded near the rabbit femoral bone,the ectopic bone constructs were harvested at 8th week for further examination and study.Results:At 8th week,the ectopic bone had a clear bone-like structure,together with new blood supply,green fluorescent protein could be seen in samples seeded by marked cells.Conclusions:This study showed that the ectopic bone formation could be successful in vivo.
引文
[1]FLORCZYK S J,LEUNG M,LI Z,et al.Evaluation of three-dimensional porous chitosan-alginate scaffolds in rat calvarial defects for bone regeneration applications[J].J Biomed Mater Res A,2013,101(10):2974-2983.
[2]YANG F,CHO S W,SON S M,et al.Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D[J].Biomacromolecules,2010,11(8):1909-1914.
[3]PAPADIMITROPOULOS A,SCHERBERICH A,GVEN S,et al.A 3Din vitro bone organ model using human progenitor cells[J].Eur Cell Mater,2011,21:445-458.
[4]KANG B J,RYU H H,PARK S S,et al.Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues,bone marrow,umbilical cord blood,and Wharton's jelly for treating bone defects[J].J Vet Sci,2012,13(3):299-310.
[5]BYEON Y E,RYU H H,PARK S S,et al.Paracrine effect of canine allogenic umbilical cord blood-derived mesenchymal stromal cells mixed with beta-tricalcium phosphate on bone regeneration in ectopic implantations[J].Cytotherapy,2010,12(5):626-636.
[6]CAPLAN A I.New era of cell-based orthopedic therapies[J].Tissue Eng Part B Rev,2009,15(2):195-200.
[7]VICTORELLI G,ALOISE A C,PASSADOR-SANTOS F,et al.Ectopic implantation of hydroxyapatite xenograft scaffold loaded with bone marrow aspirate concentrate or osteodifferentiated bone marrow mesenchymal stem cells:a pilot study in mice[J].Int J Oral Maxillofac Implants,2016,31(2):e18-e23.
[8]LIN R Z,MORENO-LUNA R,LI D,et al.Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling[J].Proc Natl Acad Sci USA,2014,111(28):10137-10142.
[9]XIE F,TENG L,WANG Q,et al.Ectopic osteogenesis of allogeneic bone mesenchymal stem cells loading onβ-tricalcium phosphate in canines[J].Plast Reconstr Surg,2014,133(2):142e-153e.
[10]DE BARI C,DELL'ACCIO F,KARYSTINOU A,et al.A biomarker-based mathematical model to predict bone-forming potency of human synovial and periosteal mesenchymal stem cells[J].Arthritis Rheum,2008,58(1):240-250.
[11]CHAI Y C,GERIS L,BOLANDER J,et al.in vivo ectopic bone formation by devitalized mineralized stem cell carriers produced under mineralizing culture condition[J].Biores Open Access,2014,3(6):265-277.
[12]TODOROV A,KREUTZ M,HAUMER A,et al.Fatderived stromal vascular fraction cells enhance the boneforming capacity of devitalized engineered hypertrophic cartilage matrix[J].Stem Cells Transl Med,2016,5(12):1684-1694.
[13]KUSUMA G D,MENICANIN D,GRONTHOS S,et al.Ectopic bone formation by mesenchymal stem cells derived from human term placenta and the decidua[J].PLoS One,2015,10(10):e0141246.
[2]YANG F,CHO S W,SON S M,et al.Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D[J].Biomacromolecules,2010,11(8):1909-1914.
[3]PAPADIMITROPOULOS A,SCHERBERICH A,GVEN S,et al.A 3Din vitro bone organ model using human progenitor cells[J].Eur Cell Mater,2011,21:445-458.
[4]KANG B J,RYU H H,PARK S S,et al.Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues,bone marrow,umbilical cord blood,and Wharton's jelly for treating bone defects[J].J Vet Sci,2012,13(3):299-310.
[5]BYEON Y E,RYU H H,PARK S S,et al.Paracrine effect of canine allogenic umbilical cord blood-derived mesenchymal stromal cells mixed with beta-tricalcium phosphate on bone regeneration in ectopic implantations[J].Cytotherapy,2010,12(5):626-636.
[6]CAPLAN A I.New era of cell-based orthopedic therapies[J].Tissue Eng Part B Rev,2009,15(2):195-200.
[7]VICTORELLI G,ALOISE A C,PASSADOR-SANTOS F,et al.Ectopic implantation of hydroxyapatite xenograft scaffold loaded with bone marrow aspirate concentrate or osteodifferentiated bone marrow mesenchymal stem cells:a pilot study in mice[J].Int J Oral Maxillofac Implants,2016,31(2):e18-e23.
[8]LIN R Z,MORENO-LUNA R,LI D,et al.Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling[J].Proc Natl Acad Sci USA,2014,111(28):10137-10142.
[9]XIE F,TENG L,WANG Q,et al.Ectopic osteogenesis of allogeneic bone mesenchymal stem cells loading onβ-tricalcium phosphate in canines[J].Plast Reconstr Surg,2014,133(2):142e-153e.
[10]DE BARI C,DELL'ACCIO F,KARYSTINOU A,et al.A biomarker-based mathematical model to predict bone-forming potency of human synovial and periosteal mesenchymal stem cells[J].Arthritis Rheum,2008,58(1):240-250.
[11]CHAI Y C,GERIS L,BOLANDER J,et al.in vivo ectopic bone formation by devitalized mineralized stem cell carriers produced under mineralizing culture condition[J].Biores Open Access,2014,3(6):265-277.
[12]TODOROV A,KREUTZ M,HAUMER A,et al.Fatderived stromal vascular fraction cells enhance the boneforming capacity of devitalized engineered hypertrophic cartilage matrix[J].Stem Cells Transl Med,2016,5(12):1684-1694.
[13]KUSUMA G D,MENICANIN D,GRONTHOS S,et al.Ectopic bone formation by mesenchymal stem cells derived from human term placenta and the decidua[J].PLoS One,2015,10(10):e0141246.