抗原浓缩技术研究进展
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摘要
动物疫病是危害养殖业发展的重要因素,而疫苗免疫又是目前防控动物疫病最有效的手段之一。为了提高兽用多联疫苗的免疫效力,去除杂质,减少临床上的免疫不良反应,需要对抗原进行浓缩。疫苗的抗原浓缩技术主要是通过物理或化学方法去除杂质以提高单位体积抗原含量,同时要防止抗原的免疫原性受到破坏,对整个浓缩条件要求较高,因此如何选择合适且高效的抗原浓缩方法尤为重要。文章对近年来抗原浓缩技术的研究及现状进行总结,旨在为选择抗原浓缩技术提供参考。
Animal epidemic is the main factor which seriously threaten to the animal industry, and vaccine is the primary way to prevent from infection.To promote the efficacy of animal multi-vaccines as well as reduce clinical immunological adverse reaction, the antigens should be purified and concentrated. The antigen concentration technology mainly removes impurities by physical or chemical methods to increase the unit volume antigen content and prevent the immunogenicity of antigens from being destroyed. Therefore, how to select the optimal antigen enrichment method is particularly significant. In this review, the present situation of research on antigen enrichment technology in recent years were summarized, aiming to provide reference for its application.
Animal epidemic is the main factor which seriously threaten to the animal industry, and vaccine is the primary way to prevent from infection.To promote the efficacy of animal multi-vaccines as well as reduce clinical immunological adverse reaction, the antigens should be purified and concentrated. The antigen concentration technology mainly removes impurities by physical or chemical methods to increase the unit volume antigen content and prevent the immunogenicity of antigens from being destroyed. Therefore, how to select the optimal antigen enrichment method is particularly significant. In this review, the present situation of research on antigen enrichment technology in recent years were summarized, aiming to provide reference for its application.
引文
[1] 张益民, 陈明朗, 陈溥言. 生物制品浓缩粗提的理化技术[J]. 畜牧与兽医, 1996(6):277-280.
[2] SARASWAT M, MUSANTE L, RAVIDá A, et al. Preparative purification of recombinant proteins: current status and future trends [J]. Biomed Res Int,2013, 2013(4):312709.
[3] BRUNELLE J L, GREEN R. Chapter twelve-one-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE)[J]. Methods Enzymol, 2014, 541:151-159.
[4] 李峰.真空冷冻干燥技术在生物制药方面的应用[J].化工管理,2017(6):132.
[5] GHALEH H, ABBASI F, ALIZADEH M, et al. Mimicking the quasi-random assembly of protein fibers in the dermis by freeze-drying method[J]. Mater Sci Eng C Mater Biol Appl, 2015, 49:807-815.
[6] 赵兰珍,李燊,李凤娟,等. 冻干工艺对聚合人胎盘血红蛋白质量影响的研究[J].生物医学工程杂志,2013,30(5):1052-1057.
[7] RAINER M, BONN G K. Enrichment of phosphorylated peptides and proteins by selective precipitation methods [J]. Bioanalysis, 2015, 7(2):243-252.
[8] RATHORE A S, SHIRKE A. Recent developments in membrane-based separations in biotechnology processes: review[J]. Prep Biochem Biotechnol, 2011, 41(4):398-421.
[9] CHRISTY C, ADAMS G, KURIYEL R, et al. High-performance tangential flow filtration: a highly selective membrane separation process [J]. Desalination, 2002, 144(1):133-136.
[10] ZHAN F C. Protein separation using ultrafiltration-an example of multi-scale complex systems[J].Particuology, 2005, 3(6):343-348.
[11] KOMMAREDDY S, BONIFICIO A, GALLORINI S, et al. Preparation of highly concentrated influenza vaccine for use in novel delivery approaches [J]. J Pharm Sci, 2013, 102(3):866-875.
[12] 王晓辉, 龙润乡, 张名, 等. HAV病毒液的3种浓缩方法比较[J]. 现代生物医学进展, 2014, 14(24):4616-4619.
[13] 陈丽萍, 江兴华. 禽流感疫苗抗原浓缩工艺的研究[J]. 福建畜牧兽医, 2017, 39(4):5-7.
[14] BAEZA-BAEZA J J, GARCíA-áLVAREZ-COQUE M C. A theoretical plate model accounting for slow kinetics in chromatographic elution[J]. J Chromatogr A, 2011, 1218(31):5166-5174.
[15] COSKUN O. Separation techniques: chromatography[J]. North Clin Istanb, 2016, 3(2):156-160.
[16] FEKETE S, BECK A, VEUTHEY J L, et al. Ion-exchange chromatography for the characterization of biopharmaceuticals[J]. J Pharm Biomed Anal, 2015, 113:43-55.
[17] HAGE D S, ANGUIZOLA J A, BI C, et al. Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments[J]. J Pharm Biomed Anal, 2012, 69:93-105.
[18] NYGREN P. Affinity ligands from biological combinatorial libraries[J]. Methods Biochem Anal, 2011, 54:269-278.
[19] ARAKAWA T, KITA Y, EJIMA D, et al. Solvent modulation of column chromatograph [J]. Protein Pept Lett, 2008, 15(6):544-555.
[20] KONG Y, LI X, BAI G, et al. An automatic system for multidimensional integrated protein chromatography [J]. J Chromatogr A, 2010, 1217(44):6898-6904.
[21] ASANZHANOVA N N, RYSKELDINOVA S Z, CHERVYAKOVA O V, et al. Comparison of different methods of purification and concentration in production of influenza vaccine[J]. Bull Exp Biol Med, 2017, 164(2):229-232.
[2] SARASWAT M, MUSANTE L, RAVIDá A, et al. Preparative purification of recombinant proteins: current status and future trends [J]. Biomed Res Int,2013, 2013(4):312709.
[3] BRUNELLE J L, GREEN R. Chapter twelve-one-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE)[J]. Methods Enzymol, 2014, 541:151-159.
[4] 李峰.真空冷冻干燥技术在生物制药方面的应用[J].化工管理,2017(6):132.
[5] GHALEH H, ABBASI F, ALIZADEH M, et al. Mimicking the quasi-random assembly of protein fibers in the dermis by freeze-drying method[J]. Mater Sci Eng C Mater Biol Appl, 2015, 49:807-815.
[6] 赵兰珍,李燊,李凤娟,等. 冻干工艺对聚合人胎盘血红蛋白质量影响的研究[J].生物医学工程杂志,2013,30(5):1052-1057.
[7] RAINER M, BONN G K. Enrichment of phosphorylated peptides and proteins by selective precipitation methods [J]. Bioanalysis, 2015, 7(2):243-252.
[8] RATHORE A S, SHIRKE A. Recent developments in membrane-based separations in biotechnology processes: review[J]. Prep Biochem Biotechnol, 2011, 41(4):398-421.
[9] CHRISTY C, ADAMS G, KURIYEL R, et al. High-performance tangential flow filtration: a highly selective membrane separation process [J]. Desalination, 2002, 144(1):133-136.
[10] ZHAN F C. Protein separation using ultrafiltration-an example of multi-scale complex systems[J].Particuology, 2005, 3(6):343-348.
[11] KOMMAREDDY S, BONIFICIO A, GALLORINI S, et al. Preparation of highly concentrated influenza vaccine for use in novel delivery approaches [J]. J Pharm Sci, 2013, 102(3):866-875.
[12] 王晓辉, 龙润乡, 张名, 等. HAV病毒液的3种浓缩方法比较[J]. 现代生物医学进展, 2014, 14(24):4616-4619.
[13] 陈丽萍, 江兴华. 禽流感疫苗抗原浓缩工艺的研究[J]. 福建畜牧兽医, 2017, 39(4):5-7.
[14] BAEZA-BAEZA J J, GARCíA-áLVAREZ-COQUE M C. A theoretical plate model accounting for slow kinetics in chromatographic elution[J]. J Chromatogr A, 2011, 1218(31):5166-5174.
[15] COSKUN O. Separation techniques: chromatography[J]. North Clin Istanb, 2016, 3(2):156-160.
[16] FEKETE S, BECK A, VEUTHEY J L, et al. Ion-exchange chromatography for the characterization of biopharmaceuticals[J]. J Pharm Biomed Anal, 2015, 113:43-55.
[17] HAGE D S, ANGUIZOLA J A, BI C, et al. Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments[J]. J Pharm Biomed Anal, 2012, 69:93-105.
[18] NYGREN P. Affinity ligands from biological combinatorial libraries[J]. Methods Biochem Anal, 2011, 54:269-278.
[19] ARAKAWA T, KITA Y, EJIMA D, et al. Solvent modulation of column chromatograph [J]. Protein Pept Lett, 2008, 15(6):544-555.
[20] KONG Y, LI X, BAI G, et al. An automatic system for multidimensional integrated protein chromatography [J]. J Chromatogr A, 2010, 1217(44):6898-6904.
[21] ASANZHANOVA N N, RYSKELDINOVA S Z, CHERVYAKOVA O V, et al. Comparison of different methods of purification and concentration in production of influenza vaccine[J]. Bull Exp Biol Med, 2017, 164(2):229-232.