H9N2亚型禽流感病毒HA检测片段阳性质粒的构建
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摘要
本试验按照农业部NYT 772─2013标准构建了长度为487 bp的H9N2亚型禽流感病毒HA检测片段阳性质粒,并对该阳性质粒的特异性、敏感性和重复性进行了鉴定,获得了可用于检测H9N2亚型禽流感病毒HA片段的阳性质粒。该质粒在-20℃下即可保存,使用方便,不会污染环境,不存在散播病毒的危险。
According to the NYT 772─2013 standard issued by Ministry of Agriculture,we constructed a 487-bp-long positive plasmid for detecting the HA fragment of H9 N2 subtype of avian influenza virus,identified the specificity,sensitivity and repeatability of this positive plasmid,and successfully obtained the positive plasmid which can be used to detect the HA fragment of H9 N2-subtype avian influenza virus. This positive plasmid can be stored at-20 ℃ and is easy to be used,and it has not dangers of spreading virus and polluting the environment.
According to the NYT 772─2013 standard issued by Ministry of Agriculture,we constructed a 487-bp-long positive plasmid for detecting the HA fragment of H9 N2 subtype of avian influenza virus,identified the specificity,sensitivity and repeatability of this positive plasmid,and successfully obtained the positive plasmid which can be used to detect the HA fragment of H9 N2-subtype avian influenza virus. This positive plasmid can be stored at-20 ℃ and is easy to be used,and it has not dangers of spreading virus and polluting the environment.
引文
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[13]宋晓辉,王中力,陈西钊.禽流感诊断研究进展[J].中国动物检疫,2003,20(11):45-47.
[2]Webster R G,Bean W J,Gorman O T,et al. Evolutionand ecology of influenza A viruses[J]. Microbiol Rev,1992,56(1):152-179.
[3]甘孟侯,于康震,徐百万,等.禽流感[M].第二版.北京:中国农业出版社,2002:80-81.
[4]Zhu X,Yu W,Mcbride R,et al. Hemagglutinin homo-logue from H17N10 bat influenza virus exhibits divergentreceptor-binding and pH-dependent fusion activities[J].Proc Natl Acad Sci,2013,110(4):1458-1463.
[5]Wu Y,Wu Y,Tefsen B,et al. T bat-derived influenza-like viruses H17N10 and HI8N11[J]. Trends Microbiol,2014,22(4):183-91.
[6]Perdue M L,Suarez D L. Structural features of the avianinfluenza virus hemagglutinin that influence virulence[J].Vet Microbiol,2000,74(1-2):77-86.
[7]Higginson R,Davies K. The threat of an avian influenzapandemic[J]. Br J Nurs,2005,14(12):632.
[8]Zeitlin G A,Maslow M J. Avian influenza[J]. Curr Al-lergy Asthma Rep,2006,6(2):163-170.
[9]郭元吉,谢健屏,吴昆昱,等.流感病毒A/广州/333/99(H9N2)毒株基因组特性的研究[J].中华实验和临床病毒学杂志,2002,16(2):142-145.
[10]Guo Y J. Influenza activity in China:1998~1999[J].Vaccine,2002(Suppl 2):28-35.
[11]陈伯伦,张泽纪,陈伟斌.禽流感研究I:鸡A型禽流感病毒的分离与血清学初步鉴定[J].中国兽医杂志,1994,20(10):3-5.
[12]郭元吉,李建国,程小雯,等.禽H9N2亚型流感病毒能感染人的发现[J].中华实验和临床病毒学杂志,1999,13:105-108.
[13]宋晓辉,王中力,陈西钊.禽流感诊断研究进展[J].中国动物检疫,2003,20(11):45-47.