臭氧胁迫及恢复过程中葡萄叶片光系统活性的变化
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摘要
为探究臭氧(O3)胁迫及恢复过程中葡萄叶片光系统活性的响应,以1年生‘赤霞珠’葡萄(Vitis vinifera L.‘Cabernet Sauvignon’)为试材,设置臭氧+弱光(90 nL·L~(-1) O_3+10μmol·m~(-2)·s~(-1))、臭氧+适光(90 nL·L~(-1)+800μmol·m~(-2)·s~(-1))、臭氧+强光(90 nL·L~(-1)+1 600μmol·m~(-2)·s~(-1))3种处理,均处理3 h,以大气臭氧浓度、适光处理(10 nL·L~(-1)+800μmol·m~(-2)·s~(-1))作为对照,分别测定处理后1、5、9和13 h的叶片气体交换参数,同时利用Dual-PAM100荧光仪测定叶片PSⅠ及PSⅡ光系统活性分析臭氧胁迫及恢复过程中光系统活性的响应。结果表明:臭氧胁迫可以降低葡萄净光合速率和气孔导度,同时降低了叶片的Y(Ⅰ)和Y(Ⅱ)值,在相同臭氧浓度下,随着光照增强叶片受到的伤害加大。恢复13 h后,臭氧弱光和臭氧适光处理的叶片净光合速率、Y(Ⅰ)和Y(Ⅱ)值回升,但臭氧强光处理的叶片伤害不断加深,光系统活性不能得到恢复。
To study the response of light system activity in grapevine leaves during ozone(O3)stress and recovery period,one-year old Vitis vinifera L.‘Cabernet Sauvignon'was used as material to be treated with ozone and low light(90 nL · L~(-1) + 10 μmol · m~(-2) · s~(-1)),ozone and light(90 nL · L~(-1) + 800 μmol · m~(-2) · s~(-1)),ozone and strong light(90 nL · L~(-1) + 1 600 μmol · m~(-2) · s~(-1)). Normal light(10 nL · L~(-1) O_3 + 800 μmol · m~(-2) · s~(-1))was as the control. The gas exchange parameters of grape leaves at 1,5,9 and 13 h after treatment were determined,and the photosystem activities of PSⅠand PSⅡ in grape leaves were measured using Dual-PAM100 fluorometer. The results showed that,the net photosynthetic rate and stomatal conductance of grape leaves were reduced under ozone stress,and the Y(Ⅰ) and Y(Ⅱ) values of grape leaves were reduced at the same time. Under the same ozone concentration,the damage of grape leaves increased with the increase of light intensity. The net photosynthetic rate,Y(Ⅰ) and Y(Ⅱ) values of dark ozone and light-resistant ozone could be increased at 13 hours after recovery,but the damage of leaves under ozone and strong light became more serious at 13 hours after recovery,and could not be recovered.
To study the response of light system activity in grapevine leaves during ozone(O3)stress and recovery period,one-year old Vitis vinifera L.‘Cabernet Sauvignon'was used as material to be treated with ozone and low light(90 nL · L~(-1) + 10 μmol · m~(-2) · s~(-1)),ozone and light(90 nL · L~(-1) + 800 μmol · m~(-2) · s~(-1)),ozone and strong light(90 nL · L~(-1) + 1 600 μmol · m~(-2) · s~(-1)). Normal light(10 nL · L~(-1) O_3 + 800 μmol · m~(-2) · s~(-1))was as the control. The gas exchange parameters of grape leaves at 1,5,9 and 13 h after treatment were determined,and the photosystem activities of PSⅠand PSⅡ in grape leaves were measured using Dual-PAM100 fluorometer. The results showed that,the net photosynthetic rate and stomatal conductance of grape leaves were reduced under ozone stress,and the Y(Ⅰ) and Y(Ⅱ) values of grape leaves were reduced at the same time. Under the same ozone concentration,the damage of grape leaves increased with the increase of light intensity. The net photosynthetic rate,Y(Ⅰ) and Y(Ⅱ) values of dark ozone and light-resistant ozone could be increased at 13 hours after recovery,but the damage of leaves under ozone and strong light became more serious at 13 hours after recovery,and could not be recovered.
引文
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Baker N R.2008.Chlorophyll fluorescence:a probe of photosynthesis in vivo.Annual Review of Plant Biology,59:89-113.
Degl’Innocenti E,Guidi L,Soldatini G F.2007.Effects of elevated ozone on chlorophyll a fluorescence in symptomatic and asymptomatic leaves of two tomato genotypes.Biologia Plantarum,51(2):313-321.
Garstka M,Venema J H,Rumak I,Gieczewska K,Rosiak M,KoziolLipinska J,Kierdaszuk B,Vredenberg W J,Mostowska J.2007.Contrasting effect of dark-chilling on chloroplast structure and arrangement of chlorophyll-protein complexes in pea and tomato:plants with a different susceptibility to non-freezing temperature.Planta,226:1165-1181.
Geng Q W,Xing H,Sun Y J,Hao G M,Zhai H,Du Y P.2017.Analysis of the interaction effects of light and O3 on fluorescence properties of‘Cabernet Sauvignon’grapes based on response surface methodology.Scientia Horticulturae,225:599-606.
Geng Qing-wei,Xing Hao,Hao Gui-mei,Sun Yong-jiang,Zhai Heng,Du Yuan-peng.2016.Effect of exogenous melatonin on photosynthesis of‘Cabernet Sauvigon’grape leaves under ozone stress.Acta Horticulturae Sinica,43(8):1463-1472.(in Chinese)耿庆伟,邢浩,郝桂梅,孙永江,翟衡,杜远鹏.2016.外源褪黑素对臭氧胁迫下‘赤霞珠’葡萄叶片光合作用的影响.园艺学报,43(8):1463-1472.
Gerganova M,Popova A V,Stanoeva D,Velitchkova M.2016.Tomato plants acclimate better to elevated temperature and high light than to treatment with each factor separately.Plant Physiology&Biochemistry,104:234-241.
Gilmore A M.2006.Mechanistic aspects of xanthophyll cycle-dependent photoprotection in higher plant chloroplasts and leaves.Physiologia Plantarum,54(3):292-302.
Huang W,Yang Y J,Zhang J L,Hu H,Zhang S B.2017.Superoxide generated in the chloroplast stroma causes photoinhibition of photosystemⅠin the shade-establishing tree species Psychotria henryi.Photosynthesis Research,132(3):293-303.
Iriti M,Faoro F.2008.Oxidative stress,the paradigm of ozone toxicity in plants and animals.Water Air&Soil Pollution,187(1-4):285-301.
Jiang Y,Huang L F,Cheng F,Zhou Y H,Xia X J,Mao W H,Shi K,Yu J Q.2013.Brassinosteroids accelerate recovery of photosynthetic apparatus from cold stress by balancing the electron partitioning,carboxylation and redox homeostasis in cucumber.Physiologia Plantarum,148(1):133-145.
Klughammer C,Schreiber U.1994.An improved method,using saturating light pulses,for the determination of photosystemⅠquantum yield via p700+-absorbance changes at 830 nm.Planta,192(2):261-268.
Kramer D M,Johnson G,Kiirats O,Edwards G E.2004.New fluorescence parameters for the determination of qaredox state and excitation energy fluxes.Photosynthesis Research,79(2):209-218.
Krupa S,Mcgrath M T,Andersen C P,Booker F L,Burkey K O,Chappelka A H,Chevone B I,Pell E J,Zilinskas B A.2001.Ambient ozone and plant health.Plant Disease,85:4-12.
Li Peng-min,Gao Hui-yuan,Reto J S.2005.Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study.Journal of Plant Physiology and Molecular Biology,(6):559-566.(in Chinese)李鹏民,高辉远,Reto J S.2005.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用.植物生理与分子生物学学报,(6):559-566.
Li Zhi-zhen,Liu Dong-huan,Zhao Shi-wei,Jiang Chuang-dao,Shi Lei.2014.Mechanisms of photoinhibition induced by high light in Hosta grown outdoors.Chinese Journal of Plant Ecology,38(7):720-728.(in Chinese)李志真,刘东焕,赵世伟,姜闯道,石雷.2014.环境强光诱导玉簪叶片光抑制的机制.植物生态学报,38(7):720-728.
Mohanty P,Allakhverdiev S I,Murata N.2007.Application of low temperatures during photoinhibition allows characterization of individual steps in photodamage and the repair of photosystem II.Photosynthesis Research,94(2-3):217-224.
Murata N,Takahashi S,Nishiyama Y,Allakhverdiev S I.2007.Photoinhibition of photosystem II under environmental stress.Biochimica et Biophysica Acta,1767(6):414-421.
Pospí?il P.2009.Production of reactive oxygen species by photosystem II.Biochimica et Biophysica Acta,1787(10):1151-1160.
Powles S B.1984.Photoinhibition of photosynthesis induced by visible light.Annual Review of Plant Biology,35(1):15-44.
Solomon S,Qin D,Manning M,Chen Z,Marquis M,Averyt K B,Miller H L.2007.Contribution of working groupⅠto the fourth assessment report of the intergovernmental panel on climate change.New York:Cambridge University Press:94-98.
Sonoike K.1996.Degradation of psab,gene product,the reaction center subunit of photosystem I,is caused during photoinhibition of photosystem I:possible involvement of active oxygen species.Plant Science,115(2):157-164.
Sonoike K.2011.Photoinhibition of photosystem I.Physiologia Plantarum,142(1):56-64.
Strasser B J.1997.Donor side capacity of photo system II probed by chlorophyll a fluorescence transients.Photosynthesis Research,52:147-155.
Sun Yong-jiang,Fu Yan-dong,Du Yuan-peng,Zhai Heng.2013.Effects of different temperature and light treatments on photosynthetic system II in Vitis vinifera‘Cabernet Sauvignon’.Scientia Agricultura Sinica,(6):1191-1200.(in Chinese)孙永江,付艳东,杜远鹏,翟衡.2013.不同温度/光照组合对‘赤霞珠’葡萄叶片光系统Ⅱ功能的影响.中国农业科学,(6):1191-1200.
Sun Yong-jiang,Wang Jin-huan,Geng Qing-wei,Xing Hao,Zhai Heng,Du Yuan-peng.2015.Effects of different concentrations of ozone stress on photosynthetic system II in Vitis vinifera cv.‘Cabernet Sauvignon’.Plant Physiology Communications,(11):1947-1954.(in Chinese)孙永江,王金欢,耿庆伟,邢浩,翟衡,杜远鹏.2015.不同浓度臭氧处理对‘赤霞珠’葡萄叶片光系统Ⅱ功能的影响.植物生理学报,(11):1947-1954.
Wang Jin-huan,Geng Qing-wei,Sun Yong-jiang,Wang Yang,Zhai Heng,Du Yuan-peng.2015.Responses of primary photochemical reactions in the leaves of three grape cultivars to the ozone stress.Sino-Overseas Grapevine&Wine,(2):16-20.(in Chinese)王金欢,耿庆伟,孙永江,王杨,翟衡,杜远鹏.2015.臭氧胁迫对3个葡萄品种叶片原初光化学反应的影响.中外葡萄与葡萄酒,(2):16-20.
Wang Jin-huan,Geng Qing-wei,Xing Hao,Sun Yong-jiang,Wang Yang,Zhai Heng,Du Yuan-peng.2016.Effects of shading on photosynthesis and reactive oxygen metabolism in Vitis vinifera‘Cabernet Sauvignon’leaves under ozone stress.Journal of Fruit Science,(7):823-831.(in Chinese)王金欢,耿庆伟,邢浩,孙永江,王杨,翟衡,杜远鹏.2016.遮阴对臭氧胁迫下‘赤霞珠’葡萄叶片光合功能及活性氧代谢的影响.果树学报,(7):823-831.
Wang Qiang,Zhang Qi-de,Zhu Xin-guang,Lu Cong-ming,Kuang Yan-yun,Li Cheng-quan.2002.PSⅡphotochemistry and xanthophyll cycle in two superhigh-yield rice hybrids,Liangyoupeijiu and Hua-an 3 during photoinhibition and subsequent restoration.Acta Botanica Sinica,11:1297-1302.
Xu Da-quan.2002.Photosynthetic efficiency.Shanghai:Shanghai Scientific and Technical Publishers:39-45.(in Chinese)许大全.2002.光合作用效率.上海:上海科学技术出版社:39-45.
Yao Qing-qun,Xie Gui-shui.2005.The photosynthetic stomatal and nonstomatal limitation under drought stress.Chinese Journal of Tropical Agriculture,25(4):84-89.(in Chinese)姚庆群,谢贵水.2005.干旱胁迫下光合作用的气孔与非气孔限制.热带农业科学,25(4):84-89.
Zhang S,Scheller H V.2004.Photoinhibition of photosystem I at chilling temperature and subsequent recovery in Arabidopsis thaliana.Plant&Cell Physiology,45(11):1595-1602.
Zhang Wei-wei,Niu Jun-feng,Wang Xiao-ke,Tian Yuan,Yao Fang-fang,Feng Zhao-zhong.2011.Effects of elevated ozone concentration on Slash Pine(Pinus elliottii)seedlings.Environmental Science,32(6):1710-1716.(in Chinese)张巍巍,牛俊峰,王效科,田媛,姚芳芳,冯兆忠.2011.大气臭氧浓度增加对湿地松幼苗的影响.环境科学,32(6):1710-1716.
Zhang Z,Jia Y,Gao H,Zhang L,Li H,Meng Q.2011.Characterization of PSⅠrecovery after chilling-induced photoinhibition incucumber(Cucumis sativus L.)leaves.Planta,234(5):883-889.
Zhang Zi-shan,Yang Cheng,Gao Hui-yuan.2013.Chilling photoinhibition of photosystemⅠand its recovery after photoinhibition.Plant Physiology Communications,49(4):301-308.(in Chinese)张子山,杨程,高辉远.2013.植物光系统Ⅰ的低温光抑制及恢复.植物生理学报,49(4):301-308.
Zhang Zi-shan,Zhang Li-tao,Gao Hui-yuan,Jia Yu-jiao,Bu Jian-wen,Meng Qing-wei.2009.Research of the photoinhibition of PSⅠand PSⅡin leaves of cucumber under chilling stress combined with different light intensities.Scientia Agricultura Sinica,42(12):4288-4293.(in Chinese)张子山,张立涛,高辉远,贾裕娇,部建雯,孟庆伟.2009.不同光强与低温交叉胁迫下黄瓜PSⅠ与PSⅡ的光抑制研究.中国农业科学,42(12):4288-4293.
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Baker N R.2008.Chlorophyll fluorescence:a probe of photosynthesis in vivo.Annual Review of Plant Biology,59:89-113.
Degl’Innocenti E,Guidi L,Soldatini G F.2007.Effects of elevated ozone on chlorophyll a fluorescence in symptomatic and asymptomatic leaves of two tomato genotypes.Biologia Plantarum,51(2):313-321.
Garstka M,Venema J H,Rumak I,Gieczewska K,Rosiak M,KoziolLipinska J,Kierdaszuk B,Vredenberg W J,Mostowska J.2007.Contrasting effect of dark-chilling on chloroplast structure and arrangement of chlorophyll-protein complexes in pea and tomato:plants with a different susceptibility to non-freezing temperature.Planta,226:1165-1181.
Geng Q W,Xing H,Sun Y J,Hao G M,Zhai H,Du Y P.2017.Analysis of the interaction effects of light and O3 on fluorescence properties of‘Cabernet Sauvignon’grapes based on response surface methodology.Scientia Horticulturae,225:599-606.
Geng Qing-wei,Xing Hao,Hao Gui-mei,Sun Yong-jiang,Zhai Heng,Du Yuan-peng.2016.Effect of exogenous melatonin on photosynthesis of‘Cabernet Sauvigon’grape leaves under ozone stress.Acta Horticulturae Sinica,43(8):1463-1472.(in Chinese)耿庆伟,邢浩,郝桂梅,孙永江,翟衡,杜远鹏.2016.外源褪黑素对臭氧胁迫下‘赤霞珠’葡萄叶片光合作用的影响.园艺学报,43(8):1463-1472.
Gerganova M,Popova A V,Stanoeva D,Velitchkova M.2016.Tomato plants acclimate better to elevated temperature and high light than to treatment with each factor separately.Plant Physiology&Biochemistry,104:234-241.
Gilmore A M.2006.Mechanistic aspects of xanthophyll cycle-dependent photoprotection in higher plant chloroplasts and leaves.Physiologia Plantarum,54(3):292-302.
Huang W,Yang Y J,Zhang J L,Hu H,Zhang S B.2017.Superoxide generated in the chloroplast stroma causes photoinhibition of photosystemⅠin the shade-establishing tree species Psychotria henryi.Photosynthesis Research,132(3):293-303.
Iriti M,Faoro F.2008.Oxidative stress,the paradigm of ozone toxicity in plants and animals.Water Air&Soil Pollution,187(1-4):285-301.
Jiang Y,Huang L F,Cheng F,Zhou Y H,Xia X J,Mao W H,Shi K,Yu J Q.2013.Brassinosteroids accelerate recovery of photosynthetic apparatus from cold stress by balancing the electron partitioning,carboxylation and redox homeostasis in cucumber.Physiologia Plantarum,148(1):133-145.
Klughammer C,Schreiber U.1994.An improved method,using saturating light pulses,for the determination of photosystemⅠquantum yield via p700+-absorbance changes at 830 nm.Planta,192(2):261-268.
Kramer D M,Johnson G,Kiirats O,Edwards G E.2004.New fluorescence parameters for the determination of qaredox state and excitation energy fluxes.Photosynthesis Research,79(2):209-218.
Krupa S,Mcgrath M T,Andersen C P,Booker F L,Burkey K O,Chappelka A H,Chevone B I,Pell E J,Zilinskas B A.2001.Ambient ozone and plant health.Plant Disease,85:4-12.
Li Peng-min,Gao Hui-yuan,Reto J S.2005.Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study.Journal of Plant Physiology and Molecular Biology,(6):559-566.(in Chinese)李鹏民,高辉远,Reto J S.2005.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用.植物生理与分子生物学学报,(6):559-566.
Li Zhi-zhen,Liu Dong-huan,Zhao Shi-wei,Jiang Chuang-dao,Shi Lei.2014.Mechanisms of photoinhibition induced by high light in Hosta grown outdoors.Chinese Journal of Plant Ecology,38(7):720-728.(in Chinese)李志真,刘东焕,赵世伟,姜闯道,石雷.2014.环境强光诱导玉簪叶片光抑制的机制.植物生态学报,38(7):720-728.
Mohanty P,Allakhverdiev S I,Murata N.2007.Application of low temperatures during photoinhibition allows characterization of individual steps in photodamage and the repair of photosystem II.Photosynthesis Research,94(2-3):217-224.
Murata N,Takahashi S,Nishiyama Y,Allakhverdiev S I.2007.Photoinhibition of photosystem II under environmental stress.Biochimica et Biophysica Acta,1767(6):414-421.
Pospí?il P.2009.Production of reactive oxygen species by photosystem II.Biochimica et Biophysica Acta,1787(10):1151-1160.
Powles S B.1984.Photoinhibition of photosynthesis induced by visible light.Annual Review of Plant Biology,35(1):15-44.
Solomon S,Qin D,Manning M,Chen Z,Marquis M,Averyt K B,Miller H L.2007.Contribution of working groupⅠto the fourth assessment report of the intergovernmental panel on climate change.New York:Cambridge University Press:94-98.
Sonoike K.1996.Degradation of psab,gene product,the reaction center subunit of photosystem I,is caused during photoinhibition of photosystem I:possible involvement of active oxygen species.Plant Science,115(2):157-164.
Sonoike K.2011.Photoinhibition of photosystem I.Physiologia Plantarum,142(1):56-64.
Strasser B J.1997.Donor side capacity of photo system II probed by chlorophyll a fluorescence transients.Photosynthesis Research,52:147-155.
Sun Yong-jiang,Fu Yan-dong,Du Yuan-peng,Zhai Heng.2013.Effects of different temperature and light treatments on photosynthetic system II in Vitis vinifera‘Cabernet Sauvignon’.Scientia Agricultura Sinica,(6):1191-1200.(in Chinese)孙永江,付艳东,杜远鹏,翟衡.2013.不同温度/光照组合对‘赤霞珠’葡萄叶片光系统Ⅱ功能的影响.中国农业科学,(6):1191-1200.
Sun Yong-jiang,Wang Jin-huan,Geng Qing-wei,Xing Hao,Zhai Heng,Du Yuan-peng.2015.Effects of different concentrations of ozone stress on photosynthetic system II in Vitis vinifera cv.‘Cabernet Sauvignon’.Plant Physiology Communications,(11):1947-1954.(in Chinese)孙永江,王金欢,耿庆伟,邢浩,翟衡,杜远鹏.2015.不同浓度臭氧处理对‘赤霞珠’葡萄叶片光系统Ⅱ功能的影响.植物生理学报,(11):1947-1954.
Wang Jin-huan,Geng Qing-wei,Sun Yong-jiang,Wang Yang,Zhai Heng,Du Yuan-peng.2015.Responses of primary photochemical reactions in the leaves of three grape cultivars to the ozone stress.Sino-Overseas Grapevine&Wine,(2):16-20.(in Chinese)王金欢,耿庆伟,孙永江,王杨,翟衡,杜远鹏.2015.臭氧胁迫对3个葡萄品种叶片原初光化学反应的影响.中外葡萄与葡萄酒,(2):16-20.
Wang Jin-huan,Geng Qing-wei,Xing Hao,Sun Yong-jiang,Wang Yang,Zhai Heng,Du Yuan-peng.2016.Effects of shading on photosynthesis and reactive oxygen metabolism in Vitis vinifera‘Cabernet Sauvignon’leaves under ozone stress.Journal of Fruit Science,(7):823-831.(in Chinese)王金欢,耿庆伟,邢浩,孙永江,王杨,翟衡,杜远鹏.2016.遮阴对臭氧胁迫下‘赤霞珠’葡萄叶片光合功能及活性氧代谢的影响.果树学报,(7):823-831.
Wang Qiang,Zhang Qi-de,Zhu Xin-guang,Lu Cong-ming,Kuang Yan-yun,Li Cheng-quan.2002.PSⅡphotochemistry and xanthophyll cycle in two superhigh-yield rice hybrids,Liangyoupeijiu and Hua-an 3 during photoinhibition and subsequent restoration.Acta Botanica Sinica,11:1297-1302.
Xu Da-quan.2002.Photosynthetic efficiency.Shanghai:Shanghai Scientific and Technical Publishers:39-45.(in Chinese)许大全.2002.光合作用效率.上海:上海科学技术出版社:39-45.
Yao Qing-qun,Xie Gui-shui.2005.The photosynthetic stomatal and nonstomatal limitation under drought stress.Chinese Journal of Tropical Agriculture,25(4):84-89.(in Chinese)姚庆群,谢贵水.2005.干旱胁迫下光合作用的气孔与非气孔限制.热带农业科学,25(4):84-89.
Zhang S,Scheller H V.2004.Photoinhibition of photosystem I at chilling temperature and subsequent recovery in Arabidopsis thaliana.Plant&Cell Physiology,45(11):1595-1602.
Zhang Wei-wei,Niu Jun-feng,Wang Xiao-ke,Tian Yuan,Yao Fang-fang,Feng Zhao-zhong.2011.Effects of elevated ozone concentration on Slash Pine(Pinus elliottii)seedlings.Environmental Science,32(6):1710-1716.(in Chinese)张巍巍,牛俊峰,王效科,田媛,姚芳芳,冯兆忠.2011.大气臭氧浓度增加对湿地松幼苗的影响.环境科学,32(6):1710-1716.
Zhang Z,Jia Y,Gao H,Zhang L,Li H,Meng Q.2011.Characterization of PSⅠrecovery after chilling-induced photoinhibition incucumber(Cucumis sativus L.)leaves.Planta,234(5):883-889.
Zhang Zi-shan,Yang Cheng,Gao Hui-yuan.2013.Chilling photoinhibition of photosystemⅠand its recovery after photoinhibition.Plant Physiology Communications,49(4):301-308.(in Chinese)张子山,杨程,高辉远.2013.植物光系统Ⅰ的低温光抑制及恢复.植物生理学报,49(4):301-308.
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