HPLC法同时测定坤安膏中芍药苷、丹酚酸B、淫羊藿苷含量
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摘要
目的:建立高效液相色谱法(HPLC)测定坤安膏中芍药苷、丹酚酸B、淫羊藿苷的含量,为质量控制提供依据。方法:色谱柱为Akasil-C_(18)柱(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液,梯度洗脱,检测波长为230nm;柱温为30℃,体积流量为1.0mL·min~(-1),进样量为10μL。结果:建立了坤安膏中芍药苷、丹酚酸B、淫羊藿苷的HPLC含量测定方法。芍药苷、丹酚酸B、淫羊藿苷的含量分别为66.80～71.47μg/g、776.60～850.94μg/g、49.80～57.14μg/g。结论:该研究建立的方法结果准确,稳定性好,简便易行,可用于坤安膏的质量控制。
Objective:To establish a(HPLC)method to determine the content of paeoniflorin,salvianolic acid B and icariin in Kun an gao,and to provide the basis for quality control.Methods:The chromatographic column was Akasil-C_(18) column(250 mm×4.6 mm,5 mu m),the mobile phase was acetonitrile-0.1% phosphoric acid solution,gradient elution,the detection wavelength was230 nm,the column temperature was 30℃,volume flow rate was 1 mL·min~(-1),and The sampling amount was 10μL.Results:A method for the determination of paeoniflorin,salvianolic acid B and icariin in Kun an ointment was established by HPLC.The contents of paeoniflorin,salvianolic acid B and icariin are 66.80～71.47μg/g,776.6～850.94μg/g,49.80～57.14μg/g respectively.Conclusion:The method established in this study is accurate,stable,simple and convenient,and can be used for quality control of Kun an gao.
Objective:To establish a(HPLC)method to determine the content of paeoniflorin,salvianolic acid B and icariin in Kun an gao,and to provide the basis for quality control.Methods:The chromatographic column was Akasil-C_(18) column(250 mm×4.6 mm,5 mu m),the mobile phase was acetonitrile-0.1% phosphoric acid solution,gradient elution,the detection wavelength was230 nm,the column temperature was 30℃,volume flow rate was 1 mL·min~(-1),and The sampling amount was 10μL.Results:A method for the determination of paeoniflorin,salvianolic acid B and icariin in Kun an ointment was established by HPLC.The contents of paeoniflorin,salvianolic acid B and icariin are 66.80～71.47μg/g,776.6～850.94μg/g,49.80～57.14μg/g respectively.Conclusion:The method established in this study is accurate,stable,simple and convenient,and can be used for quality control of Kun an gao.
引文
[1]赵先,王婧雯,陆杨,等.丹酚酸B药理作用的研究进展[J].西北药学杂志,2015,1(30):107-110.
[2]王可可,龚其海.淫羊藿化学成分及药理作用的研究进展[J].中国民族民间医药,2015(19):16-18.
[3]黄丽苹,时桂芹,陈利平,等.芍药苷提取方法及药理作用研究进展[J].农产品加工,2018(2):71-75.
[4]张乐;张强;刘娟,等.参七消痞颗粒中野黄芩苷和丹酚酸B含量测定方法研究[J].北京中医药大学学报,2013,8(36):550-553.
[5]石小鹏,缪珊,毕琳琳,等.芪生胶囊中芍药苷的含量测定[J].医药导报,2014,33(7):937-939.
[6]黄泽中,何利华,尹雄章.小儿化毒散中芍药苷含量测定[J].医药导报,2012,31(7):916-917.
[7]陶雅.HPLC法测定活血止痛膏中芍药苷的含量[J].内蒙古中医药,2012,31(3):97.
[8]朱燕,黄义纯,严晓明.高效液相色谱法同时测定抗骨增生胶囊中的毛蕊花糖苷、柚皮苷和淫羊藿苷[J].广东药学院学报,2016,32(5):589-592.
[9]陈群力,周淑琴,须冰,等.高效液相色谱法同时测定大黄灵脾颗粒中丹酚酸B和淫羊藿苷的含量[J].安徽医药,2016,20(1):46-48.
[10]王跃群.柴茵利胆合剂中芍药苷的含量测定方法研究[J].湖北中医杂志,2012,34(5):74-75.
[11]黄杰芳.防芷鼻炎片中芍药苷含量测定方法的优化[J].中国现代药物应用,2012,6(8):16-17.
[12]涂兴明,李赐恩,吴康郁.高效液相色谱法测定骨康口服液中芍药苷的含量[J].中药新药与临床药理,2013,24(6):613-615.
[13]明少兰.高效液相色谱法测定柴芍柔肝颗粒中芍药苷含量[J].医药导报,2011,30(5):655-656.
[14]任亚东,施猛,孙秋艳,等.HPLC法测定正元胶囊中淫羊藿苷的含量[J].亚太传统医药,2014,10(11):16-18.
[2]王可可,龚其海.淫羊藿化学成分及药理作用的研究进展[J].中国民族民间医药,2015(19):16-18.
[3]黄丽苹,时桂芹,陈利平,等.芍药苷提取方法及药理作用研究进展[J].农产品加工,2018(2):71-75.
[4]张乐;张强;刘娟,等.参七消痞颗粒中野黄芩苷和丹酚酸B含量测定方法研究[J].北京中医药大学学报,2013,8(36):550-553.
[5]石小鹏,缪珊,毕琳琳,等.芪生胶囊中芍药苷的含量测定[J].医药导报,2014,33(7):937-939.
[6]黄泽中,何利华,尹雄章.小儿化毒散中芍药苷含量测定[J].医药导报,2012,31(7):916-917.
[7]陶雅.HPLC法测定活血止痛膏中芍药苷的含量[J].内蒙古中医药,2012,31(3):97.
[8]朱燕,黄义纯,严晓明.高效液相色谱法同时测定抗骨增生胶囊中的毛蕊花糖苷、柚皮苷和淫羊藿苷[J].广东药学院学报,2016,32(5):589-592.
[9]陈群力,周淑琴,须冰,等.高效液相色谱法同时测定大黄灵脾颗粒中丹酚酸B和淫羊藿苷的含量[J].安徽医药,2016,20(1):46-48.
[10]王跃群.柴茵利胆合剂中芍药苷的含量测定方法研究[J].湖北中医杂志,2012,34(5):74-75.
[11]黄杰芳.防芷鼻炎片中芍药苷含量测定方法的优化[J].中国现代药物应用,2012,6(8):16-17.
[12]涂兴明,李赐恩,吴康郁.高效液相色谱法测定骨康口服液中芍药苷的含量[J].中药新药与临床药理,2013,24(6):613-615.
[13]明少兰.高效液相色谱法测定柴芍柔肝颗粒中芍药苷含量[J].医药导报,2011,30(5):655-656.
[14]任亚东,施猛,孙秋艳,等.HPLC法测定正元胶囊中淫羊藿苷的含量[J].亚太传统医药,2014,10(11):16-18.