桃红四物汤中6种指标性成分的含量测定
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摘要
目的建立超高效液相色谱法同时测定桃红四物汤中6种指标性成分(羟基红花黄色素A、苦杏仁苷、芍药苷、阿魏酸、毛蕊花糖苷、藁本内酯)的含量。方法采用Agilent Technologies 1290 Infinity超高效液相色谱系统、DAD检测器。色谱柱为Waters ACQUITY UPLC BEH C_(18)柱(2.1 mm×100 mm,1.7μm),流动相A为0.05%乙酸水(含7.84 mmol/L乙酸铵),B为甲醇溶液,采用梯度洗脱程序和多波长融合检测方法,流速0.2μL/min,进样量2μL,柱温25℃。结果 6种指标性成分的线性、分离度、稳定性、重复性与精密度良好;该方法能够快速、稳定地定量羟基红花黄色素A、苦杏仁苷、芍药苷、阿魏酸、毛蕊花糖苷、藁本内酯。结论该方法稳定、可靠、重复性高,可适用于桃红四物汤的质量控制。
Objective To establish an ultra-performance liquid chromatography(UPLC)method for the simultaneous determination of six index components in Taohong Siwu Decoction,i.e.,hydroxysafflor yellow A,amygdalin,paeoniflorin,ferulic acid,verbascoside,and ligustilide.Methods The Agilent Technologies 1290 Infinity UPLC system and a DAD detector were used for content determination.UPLC was performed on A Waters ACQUITY UPLC BEH C18 column(2.1 mm×100 mm,1.7μm),with a mobile phase A of 0.05% acetic acid water(containing 7.84 mmol/L ammonium acetate)and a mobile phase B of methanol solution for gradient elution and multi-wavelength detection,at a flow rate of 0.2μL/min,a sample size of 2μL,and a column temperature of25℃.Results All 6 components had good linearity,resolution,stability,reproducibility,and precision.This method could be used for rapid and stable quantitative determination of hydroxysafflor yellow A,amygdalin,paeoniflorin,ferulic acid,verbascoside,and ligustilide.Conclusion The method is stable and reliable with high reproducibility and can be used for the quality control of Taohong Siwu Decoction.
Objective To establish an ultra-performance liquid chromatography(UPLC)method for the simultaneous determination of six index components in Taohong Siwu Decoction,i.e.,hydroxysafflor yellow A,amygdalin,paeoniflorin,ferulic acid,verbascoside,and ligustilide.Methods The Agilent Technologies 1290 Infinity UPLC system and a DAD detector were used for content determination.UPLC was performed on A Waters ACQUITY UPLC BEH C18 column(2.1 mm×100 mm,1.7μm),with a mobile phase A of 0.05% acetic acid water(containing 7.84 mmol/L ammonium acetate)and a mobile phase B of methanol solution for gradient elution and multi-wavelength detection,at a flow rate of 0.2μL/min,a sample size of 2μL,and a column temperature of25℃.Results All 6 components had good linearity,resolution,stability,reproducibility,and precision.This method could be used for rapid and stable quantitative determination of hydroxysafflor yellow A,amygdalin,paeoniflorin,ferulic acid,verbascoside,and ligustilide.Conclusion The method is stable and reliable with high reproducibility and can be used for the quality control of Taohong Siwu Decoction.
引文
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[24] 杨艳,易进海,刘云华,等.一测多评法测定中成药中洋川芎内酯A和藁本内酯[J].中成药,2015,37(5):1000-1004.
[25] 郭小藤,赵睁睁,容蓉,等.两种方法测定川芎油中藁本内酯、洋川芎内酯A和正丁基苯酞的含量[J].中国实验方剂学杂志,2012,18(13):95-98.
[2] 韩岚,彭代银,许钒,等.桃红四物汤抗血栓形成作用研究[J].安徽中医学院学报,2010,29(1):47-49.
[3] 韩岚,彭代银,许钒,等.桃红四物汤抗血小板活化作用及机制研究[J].中国中药杂志,2010,35(19):2609-2612.
[4] 吴欢茹,韩岚,彭代银,等.脑缺血再灌注大鼠海马区神经营养因子的表达及桃红四物汤的干预[J].安徽中医药大学学报,2017,36(5):56-61.
[5] 韩岚,梁杰,张艳艳,等.桃红四物汤对产后血瘀大鼠血清NO、子宫组织VEGF的表达及对PI3K/Akt信号通路的影响[J].中华中医药杂志,2016,31(5):1625-1629.
[6] 白金,徐沛沛,翟玲玲,等.桃红四物汤对髌骨骨折患者术后康复的影响[J].中国实验方剂学杂志,2017,23(9):169-173.
[7] 陶海侠.桃红四物汤治疗痛经的临床经验及心得探析[J].临床医药文献电子杂志,2016,3(21):4290-4292.
[8] 王辰雯,彭代银.超高压液相色谱法同时测定桃红四物汤中四种主要有效成分的含量[J].安徽医药,2013,17(7):1110-1112.
[9] 李鹏,李祥,陈建伟.桃红四物汤醇提部位化学成分研究[J].南京中医药大学学报,2010,26(4):286-288.
[10] 张强,李祥,陈建伟.桃红四物汤水溶性部位的化学成分研究[J].现代中药研究与实践,2009,23(3):43-44.
[11] 杨辉,张季,刘丰熙,等.ISC-HPLC法同时测定桃红四物汤中酚类物质[J].中草药,2014,45(24):3565-3568.
[12] 邓中甲.方剂学[M].7版.北京:中国中医药出版社,2003:162.
[13] 赵文红,邓泽元,范亚苇,等.阿魏酸体外抗氧化作用研究[J].食品科学,2010,31(1):219-223.
[14] 吴建良,沈敏敏,杨水新,等.阿魏酸对小胶质细胞炎性反应的抑制作用[J].中国药理学通报,2015,31(1):97-102.
[15] 陈莉芬,胡长林,谢运兰.复方阿魏酸对实验性大鼠脑缺血再灌注损伤的保护作用[J].重庆医科大学学报,2003(1):49-52.
[16] 欧阳静萍,王保华,刘永明,等.阿魏酸钠对高脂血症家兔动脉粥样硬化形成的影响及其机制的研究[J].中国药理学通报,2002(2):207-210.
[17] 钱忠明,杜俊蓉,汪程远.藁本内酯在制备预防和治疗脑缺血性疾病的药物中的应用:中国,CN1732921A[P].2006-02-15.
[18] 刘骏,魏玉平,刘永福,等.顺式藁本内酯的提取方法及药用用途:中国,CN1552702A[P].2004-12-08.
[19] LIANG M J,HE L C,YANG G D,et al.Screening,analysis and in vitro vasodilatation of effective components from Ligusticum Chuanxiong[J].Life Sci,2005,78(2):128.
[20] MESELHY M R,KADOTA S,MOMOSE Y,et al.Two new Quinochalcone yellow pigments from Carthamus tinctorius and Ca2+ antagonistic activity of tinctormine[J].Chem Pharm Bull,1993,41(10):1796-1820.
[21] 郑世存,李晓宇,欧阳兵,等.芍药苷药理作用研究新进展[J].中国药物警戒,2012,9(2):100-103.
[22] 赵微,潘英妮.肉苁蓉苯乙醇苷类成分药理作用研究进展[J].亚太传统医药,2013,9(5):77-79.
[23] 中华人民共和国国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015.
[24] 杨艳,易进海,刘云华,等.一测多评法测定中成药中洋川芎内酯A和藁本内酯[J].中成药,2015,37(5):1000-1004.
[25] 郭小藤,赵睁睁,容蓉,等.两种方法测定川芎油中藁本内酯、洋川芎内酯A和正丁基苯酞的含量[J].中国实验方剂学杂志,2012,18(13):95-98.