补骨脂素对大鼠正畸复发的生物学影响
|
摘要
目的探讨补骨脂素对牙移动后复发的影响,及对G蛋白偶联受体30 (G protein coupled receptor30,GPR30)的作用。方法 36只大鼠随机分为两组,拉左上颌第一磨牙向近中21 d后去除加力装置,分别以补骨脂素和生理盐水灌胃28 d后处死,上颌骨行HE染色和GPR30免疫组化染色。灌胃期间定期取石膏模型计算复发距离。结果两组都复发明显,对照组在第一周后的复发程度与速度大于补骨脂素组,但仅当第1周时的复发速度差有统计学意义(实验组:(0.07±0.01)mm·d~(-1),对照组(0.12±0.01)mm·d~(-1)),P<0.05。免疫组化染色显示牙周膜、牙槽骨和细胞牙骨质中存在GPR30,表达强度上对照组低于实验组,P<0.05。结论牙周组织中存在GPR30,补骨脂素具有促进其表达和牙周组织改建,维护正畸治疗效果,降低正畸复发可能性的作用。
Objective To explore the effects of psoralen on orthodontic relapse and GPR30. Methods 36 Wistar rats were divided into two groups at random. Force appliances were removed after 21 days when the first molar in the left upper jaw was pulled to the mesiocclusion. Then the rats in two groups were given psoralen and sodium chloride by gavage respectively after 28 days. Morphological changes were observed by hematoxylin-eosin staining and the expression of GPR30 was determined by immunohistochemistry staining.The relapse distance was measured by regularly choosing plaster model during the gavage. Results Both groups had obvious relapse.Control group showed higher relapse degree and rate in the first week,but only in the first week did there exist significant difference compared to psoralen group( P<0.05). Immunohistochemistry staining showed that GPR30 existed in the parodontium,alveolar bone and cellular cementum. The expression of GPR30 in experimental group was significantly higher than control group( P<0.05). Conclusion GPR30 exists in the periodontal tissue. Psoralen has the function of promoting its expression and periodontal tissue reconstruction,maintaining orthodontic treatment effect,and reducing the possibility of orthodontic relapse.
Objective To explore the effects of psoralen on orthodontic relapse and GPR30. Methods 36 Wistar rats were divided into two groups at random. Force appliances were removed after 21 days when the first molar in the left upper jaw was pulled to the mesiocclusion. Then the rats in two groups were given psoralen and sodium chloride by gavage respectively after 28 days. Morphological changes were observed by hematoxylin-eosin staining and the expression of GPR30 was determined by immunohistochemistry staining.The relapse distance was measured by regularly choosing plaster model during the gavage. Results Both groups had obvious relapse.Control group showed higher relapse degree and rate in the first week,but only in the first week did there exist significant difference compared to psoralen group( P<0.05). Immunohistochemistry staining showed that GPR30 existed in the parodontium,alveolar bone and cellular cementum. The expression of GPR30 in experimental group was significantly higher than control group( P<0.05). Conclusion GPR30 exists in the periodontal tissue. Psoralen has the function of promoting its expression and periodontal tissue reconstruction,maintaining orthodontic treatment effect,and reducing the possibility of orthodontic relapse.
引文
[1]林鹏,郭新星,魏竹亮.局部注射黄芪多糖对大鼠正畸牙保持阶段BMP-2表达的影响[J].口腔医学研究,2017,33(5):520-524.
[2]邵航,张俐.补骨脂抗骨质疏松作用的研究进展[J].中国中医骨伤科杂志,2015,23(3):69-71.
[3]李启活.补骨脂素激活MAPK-NF-κB信号通路促进成骨细胞增殖的研究[D].广州:广州中医药大学,2016.
[4]柴丽娟,樊娜,王虹,等.补骨脂单体成分对体外培养成骨细胞和破骨细胞分化的影响[J].天津中医药,2015,32(5):299-303.
[5]Kang WB,Deng YT,Wang DS,et al.Osteoprotective effects of estrogen membrane receptor GPR30 in ovariectomized rats[J].JSteroid Biochem Mol Biol,2015,154:237-244.
[6]Luo LJ,Liu F,Lin ZK,et al.Genistein regulates the IL-1 beta induced activation of MAPKs in human periodontal ligament cells through G protein-coupled receptor 30[J].Arch Biochem Biophys,2012,522(1):9-16.
[7]张世英,刘继光,赵刚.丹参酮IIA局部注射正畸牙移动后复发阶段破骨细胞分化因子的表达[J].中国组织工程研究,2014,18(11):1730-1736.
[8]Ashizawa Y,Sahara N.Quantitative evaluation of newly formed bone in the alveolar wall surrounding the root during the initial stage of experimental tooth movement in the rat[J].Arch Oral Biol,1998,43(6):473-484.
[9]章魁华,于世凤.实验口腔医学[M].北京:人民卫生出版社,2009:3-14.
[10]李玲.黄芩苷对牙周炎大鼠牙移动保持阶段VEGF、BMP-2及IL-1β表达的影响[J].中国生化药物杂志,2015,35(3):32-35.
[11]King GJ,Keeling SD,Mc Coy EA,et al.Measuring dental drift and orthodontic tooth movement in response to various initial forces in adult rats[J].Am J Orthod Dentofacial Orthop,1991,99(5):456-465.
[12]杨琳,曾英,李劲平,等.补骨脂素对去势雌鼠E2、ERβ、TNF-α、IL-17的影响[J].中国骨质疏松杂志,2016(4):387-392,395.
[13]Yuan X,Bi Y,Yan Z,et al.Psoralen and isopsoralen ameliorate sex hormone deficiency-induced osteoporosis in female and male mice[J].Biomed Res Int,2016,2016:6869452.
[14]杨琳,曾英,李劲平,等.补骨脂素对去势骨质疏松雌鼠TGF-β的影响[J].湖南中医杂志,2016,32(2):155-157.
[15]Yang Z,Huang JH,Liu SF,et al.The osteoprotective effect of psoralen ovariectomy-induced osteoporotic rats via stimulating the osteoblastic differentiation from bone mesenchymal stem cells[J].Menopause,2012,19(10):1156-1164.
[16]Xu K,Pan X,Sun YJ,et al.Psoralen activates cartilaginous cellular functions of rat chondrocytes in vitro[J].Pharm Biotechnol,2015,53(7):1010-1015.
[17]Han G,Chen Y,Hou J,et al.Effects of simvastatin on relapse and remodeling of periodontal tissues after tooth movement in rats[J].Am J Orthod Dentofacial Orthop,2010,138(5):550.
[18]Wang Y,Wang XX,Zhang LN,et al.Effects of traditional Chinese medicine on bone remodeling during orthodontic tooth movement[J].J Ethnopharmacol,2012,141(2):642-646.
[19]Fujimura Y,Kitaura H,Yoshimatsu M,et al.Influence of bisphosphonates on orthodontic tooth movement in mice[J].Eur JOrthod,2009,31(6):572.
[20]Hirate Y,Yamaguchi M,Kasai K.Effects of relaxin on relapse and periodontal tissue remodeling after experimental tooth movement in rats[J].Connect Tissue Res,2011,53(3):207-219.
[21]Windahl SH,Andersson N,Martensson UEA,et al.Deficiency of the G protein-coupled estrogen receptor GPR30 leads to reduced bone growth and bone mineral density in female mice[J].J Bone Miner Res,2007,22:S280-S280.
[2]邵航,张俐.补骨脂抗骨质疏松作用的研究进展[J].中国中医骨伤科杂志,2015,23(3):69-71.
[3]李启活.补骨脂素激活MAPK-NF-κB信号通路促进成骨细胞增殖的研究[D].广州:广州中医药大学,2016.
[4]柴丽娟,樊娜,王虹,等.补骨脂单体成分对体外培养成骨细胞和破骨细胞分化的影响[J].天津中医药,2015,32(5):299-303.
[5]Kang WB,Deng YT,Wang DS,et al.Osteoprotective effects of estrogen membrane receptor GPR30 in ovariectomized rats[J].JSteroid Biochem Mol Biol,2015,154:237-244.
[6]Luo LJ,Liu F,Lin ZK,et al.Genistein regulates the IL-1 beta induced activation of MAPKs in human periodontal ligament cells through G protein-coupled receptor 30[J].Arch Biochem Biophys,2012,522(1):9-16.
[7]张世英,刘继光,赵刚.丹参酮IIA局部注射正畸牙移动后复发阶段破骨细胞分化因子的表达[J].中国组织工程研究,2014,18(11):1730-1736.
[8]Ashizawa Y,Sahara N.Quantitative evaluation of newly formed bone in the alveolar wall surrounding the root during the initial stage of experimental tooth movement in the rat[J].Arch Oral Biol,1998,43(6):473-484.
[9]章魁华,于世凤.实验口腔医学[M].北京:人民卫生出版社,2009:3-14.
[10]李玲.黄芩苷对牙周炎大鼠牙移动保持阶段VEGF、BMP-2及IL-1β表达的影响[J].中国生化药物杂志,2015,35(3):32-35.
[11]King GJ,Keeling SD,Mc Coy EA,et al.Measuring dental drift and orthodontic tooth movement in response to various initial forces in adult rats[J].Am J Orthod Dentofacial Orthop,1991,99(5):456-465.
[12]杨琳,曾英,李劲平,等.补骨脂素对去势雌鼠E2、ERβ、TNF-α、IL-17的影响[J].中国骨质疏松杂志,2016(4):387-392,395.
[13]Yuan X,Bi Y,Yan Z,et al.Psoralen and isopsoralen ameliorate sex hormone deficiency-induced osteoporosis in female and male mice[J].Biomed Res Int,2016,2016:6869452.
[14]杨琳,曾英,李劲平,等.补骨脂素对去势骨质疏松雌鼠TGF-β的影响[J].湖南中医杂志,2016,32(2):155-157.
[15]Yang Z,Huang JH,Liu SF,et al.The osteoprotective effect of psoralen ovariectomy-induced osteoporotic rats via stimulating the osteoblastic differentiation from bone mesenchymal stem cells[J].Menopause,2012,19(10):1156-1164.
[16]Xu K,Pan X,Sun YJ,et al.Psoralen activates cartilaginous cellular functions of rat chondrocytes in vitro[J].Pharm Biotechnol,2015,53(7):1010-1015.
[17]Han G,Chen Y,Hou J,et al.Effects of simvastatin on relapse and remodeling of periodontal tissues after tooth movement in rats[J].Am J Orthod Dentofacial Orthop,2010,138(5):550.
[18]Wang Y,Wang XX,Zhang LN,et al.Effects of traditional Chinese medicine on bone remodeling during orthodontic tooth movement[J].J Ethnopharmacol,2012,141(2):642-646.
[19]Fujimura Y,Kitaura H,Yoshimatsu M,et al.Influence of bisphosphonates on orthodontic tooth movement in mice[J].Eur JOrthod,2009,31(6):572.
[20]Hirate Y,Yamaguchi M,Kasai K.Effects of relaxin on relapse and periodontal tissue remodeling after experimental tooth movement in rats[J].Connect Tissue Res,2011,53(3):207-219.
[21]Windahl SH,Andersson N,Martensson UEA,et al.Deficiency of the G protein-coupled estrogen receptor GPR30 leads to reduced bone growth and bone mineral density in female mice[J].J Bone Miner Res,2007,22:S280-S280.