氨气和硫化氢应激对肉羊免疫及抗氧化功能的影响
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摘要
旨在研究不同浓度氨气和硫化氢应激对肉羊免疫及抗氧化功能的影响。本研究选用8只青年母羊,随机分为两组,每组4个重复,两组羊在动物营养代谢环控舱A舱、B舱内循环进行如下处理,A舱:对照(0~6d):3mg·m~(-3 )NH_3+0 mg·m~(-3 )H_2S;处理1(8~13d):20 mg·m~(-3 )NH_3+8 mg·m~(-3 )H_2S;处理2(15~20d):40mg·m~(-3 )NH_3+8mg·m~(-3 )H_2S;处理3(22~27d):80mg·m~(-3 )NH_3+8mg·m~(-3 )H_2S;B舱:处理4(8~13d):20mg·m~(-3 )NH_3+16mg·m~(-3 )H_2S;处理5(15~20d):40mg·m~(-3 )NH_3+16mg·m~(-3 )H_2S;处理6(22~27d):80mg·m~(-3 )NH_3+16mg·m~(-3 )H_2S。每个处理试验期6d。于每个处理试验期间第2和第6天采集羊只血清样,测定指标包括:免疫指标(血清免疫球蛋白、补体、溶菌酶和细胞因子含量)和抗氧化指标(血清总抗氧化能力、抗氧化酶活性和丙二醛含量)。结果表明:1)与对照组相比,入舱第2天,处理3、4血清IL-1β含量显著升高(P<0.05),处理4、6血清IL-6含量极显著升高(P<0.01);入舱第6天,试验处理组血清IgA、IgG(处理1除外)、IgM含量、血清溶菌酶含量及补体C3、C4水平极显著降低(P<0.01),处理4血清IL-1β含量显著升高(P<0.05)。H_2S浓度对入舱第2天羊血清中LZM、IL-10含量影响显著(P<0.05),对IL-6含量影响极显著(P<0.01);对入舱第6天羊血清中IL-1β含量、C4水平影响显著(P<0.05),对IgG、IgM、IL-10含量、C3水平影响极显著(P<0.01);随着入舱时间的延长,血清IgG、IgM含量、C3、C4水平呈下降的趋势;NH_3浓度与H_2S浓度对入舱第2天羊血清中IL-1β含量有显著的互作效应(P<0.05),对IgG、IL-6含量有极显著的互作效应(P<0.01);对入舱第6天羊血清中IL-1β含量有显著的互作效应(P<0.05)。2)与对照组相比,入舱第2天,处理3、4、5、6血清SOD活性极显著降低(P<0.01),处理4、5、6血清CAT活性极显著降低(P<0.01);入舱第6天,试验处理组(处理1除外)血清SOD、CAT活性极显著降低(P<0.01),处理5血清T-AOC活性显著降低(P<0.05),处理4、5、6血清MDA含量极显著升高(P<0.01)。H_2S浓度对入舱第2天羊血清中GSH-Px活性、MDA含量影响显著(P<0.05),对血清SOD、CAT活性影响极显著(P<0.01),对入舱第6天血清T-AOC、SOD、CAT活性、MDA含量影响极显著(P<0.01);NH_3浓度对入舱第2天羊血清中SOD、CAT活性影响显著(P<0.05),对入舱第6天羊血清中SOD、CAT活性影响极显著(P<0.01);随着入舱时间的延长,血清SOD、CAT活性呈下降的趋势;NH_3浓度与H_2S浓度对入舱第2天羊血清中T-AOC活性有显著的互作效应(P<0.05),对入舱第2天羊血清中SOD、CAT、GSH-Px活性有极显著的互作效应(P<0.01);对入舱第6天羊血清中SOD、CAT活性有极显著的互作效应(P<0.01)。本研究表明,NH_3和H_2S可引起肉羊应激,降低肉羊免疫功能和抗氧化能力,且随着NH_3与H_2S浓度的升高和作用时间的延长,对机体损害程度加重。
This experiment was conducted to study the effects of ammonia and hydrogen sulfide stress on immunity and antioxidant function of goats.Eight young female goats were randomly divided into two groups,and each group had 4replicates.The goats were in the separated environmental controlled chamber A and B processed by the following treatments successively.Chamber A:control group(0-6d):3 mg·m~(-3 )NH_3+0 mg·m~(-3 )H_2S;treatment 1(T1)(8-13d):20mg·m~(-3 )NH_3+8mg·m~(-3 )H_2S;treatment 2(T2)(15-20d):40mg·m~(-3 )NH_3+8mg·m~(-3)H_2S;treatment 3(T3)(22-27d):80mg·m~(-3 )NH_3+8mg·m~(-3 )H_2S.Chamber B:treatment 4(T4)(8-13d):20mg·m~(-3 )NH_3+16mg·m~(-3 )H_2S;treatment 5(T5)(15-20d):40mg·m~(-3)NH_3+16mg·m~(-3 )H_2S;treatment 6(T6)(22-27d):80mg·m~(-3 )NH_3+16mg·m~(-3 )H_2S.The serum samples of goats were collected at 2nd and 6th day of each experiment which lasted for6days,then the immune indexes(immunoglobulin,complement,lysozyme and cytokine content)and antioxidant indexes(T-AOC,antioxidant enzyme activity and MDA content)were measured.The results showed as follows:1)Compared with the control group,serum IL-1βcontent in T3,T4significantly increased(P<0.05),and serum IL-6content in T4,T6significantly increased(P<0.01)at the 2nd day;contents of serum IgA,IgG,IgM,LZM,C3and C4significantly decreased(P<0.01)in the treatments(except T1)and serum IL-1βcontent significantly increased(P<0.05)in T4at the 6th day.H_2S concentration had the significant influence on the contents of serum LZM(P<0.05),IL-10(P<0.05),IL-6(P<0.01)at the 2nd day,and the contents of serum IL-1β(P<0.05),IgG(P<0.01),IgM(P<0.01),IL-10(P<0.01),C3(P<0.01),C4(P<0.05)at the 6th day.With the increase of time in climate chambers,contents of serum IgG,IgM,C3,C4decreased.NH_3and H_2S concentrations had the significant interaction effect on contents of serum IL-1β(P<0.05),IgG(P<0.01),IL-6(P<0.01)at the2nd day and IL-1β(P<0.05)at the 6th day.2)Compared with the control group,the activities of serum SOD in T3,T4,T5,T6and CAT in T4,T5,T6significantly decreased(P<0.01)at the 2nd day;at the 6th day,the activities of serum SOD,CAT in the treatments(except T1)significantly decreased(P<0.01),the activity of serum T-AOC in T5significantly decreased(P<0.05),as well as the serum level of MDA significantly increased(P<0.01)in T4,T5,T6.H_2S concentration had the significant influence on the activities of serum GSH-Px(P<0.05),SOD(P<0.01),CAT(P<0.01)and MDA content(P<0.05)at the 2nd day,and the activities of serum T-AOC(P<0.01),SOD(P<0.01),CAT(P<0.01),MDA content(P<0.01)at the6th day.NH_3concentration also had the significant influence on the activities of serum SOD(P<0.05),CAT(P<0.05)at the 2nd day,and the activities of serum SOD(P<0.01),CAT(P<0.01)at the 6th day.With the increase of time in climate chambers,the activities of serum SOD,CAT decreased.NH_3and H_2S concentrations had the significant interaction effect on the activities of serum T-AOC(P<0.05),SOD(P<0.01),CAT(P<0.01),GSH-Px(P<0.01)at the2nd day,and the activities of serum SOD(P<0.01),CAT(P<0.01)at the 6th day.It is concluded that NH_3and H_2S could cause stress in goats and reduced the immune function and antioxidant capacity of goats.Furthermore,with the increase of NH_3and H_2S concentrations and the prolongation of the action time,the degree of damage to the goats became worse.
This experiment was conducted to study the effects of ammonia and hydrogen sulfide stress on immunity and antioxidant function of goats.Eight young female goats were randomly divided into two groups,and each group had 4replicates.The goats were in the separated environmental controlled chamber A and B processed by the following treatments successively.Chamber A:control group(0-6d):3 mg·m~(-3 )NH_3+0 mg·m~(-3 )H_2S;treatment 1(T1)(8-13d):20mg·m~(-3 )NH_3+8mg·m~(-3 )H_2S;treatment 2(T2)(15-20d):40mg·m~(-3 )NH_3+8mg·m~(-3)H_2S;treatment 3(T3)(22-27d):80mg·m~(-3 )NH_3+8mg·m~(-3 )H_2S.Chamber B:treatment 4(T4)(8-13d):20mg·m~(-3 )NH_3+16mg·m~(-3 )H_2S;treatment 5(T5)(15-20d):40mg·m~(-3)NH_3+16mg·m~(-3 )H_2S;treatment 6(T6)(22-27d):80mg·m~(-3 )NH_3+16mg·m~(-3 )H_2S.The serum samples of goats were collected at 2nd and 6th day of each experiment which lasted for6days,then the immune indexes(immunoglobulin,complement,lysozyme and cytokine content)and antioxidant indexes(T-AOC,antioxidant enzyme activity and MDA content)were measured.The results showed as follows:1)Compared with the control group,serum IL-1βcontent in T3,T4significantly increased(P<0.05),and serum IL-6content in T4,T6significantly increased(P<0.01)at the 2nd day;contents of serum IgA,IgG,IgM,LZM,C3and C4significantly decreased(P<0.01)in the treatments(except T1)and serum IL-1βcontent significantly increased(P<0.05)in T4at the 6th day.H_2S concentration had the significant influence on the contents of serum LZM(P<0.05),IL-10(P<0.05),IL-6(P<0.01)at the 2nd day,and the contents of serum IL-1β(P<0.05),IgG(P<0.01),IgM(P<0.01),IL-10(P<0.01),C3(P<0.01),C4(P<0.05)at the 6th day.With the increase of time in climate chambers,contents of serum IgG,IgM,C3,C4decreased.NH_3and H_2S concentrations had the significant interaction effect on contents of serum IL-1β(P<0.05),IgG(P<0.01),IL-6(P<0.01)at the2nd day and IL-1β(P<0.05)at the 6th day.2)Compared with the control group,the activities of serum SOD in T3,T4,T5,T6and CAT in T4,T5,T6significantly decreased(P<0.01)at the 2nd day;at the 6th day,the activities of serum SOD,CAT in the treatments(except T1)significantly decreased(P<0.01),the activity of serum T-AOC in T5significantly decreased(P<0.05),as well as the serum level of MDA significantly increased(P<0.01)in T4,T5,T6.H_2S concentration had the significant influence on the activities of serum GSH-Px(P<0.05),SOD(P<0.01),CAT(P<0.01)and MDA content(P<0.05)at the 2nd day,and the activities of serum T-AOC(P<0.01),SOD(P<0.01),CAT(P<0.01),MDA content(P<0.01)at the6th day.NH_3concentration also had the significant influence on the activities of serum SOD(P<0.05),CAT(P<0.05)at the 2nd day,and the activities of serum SOD(P<0.01),CAT(P<0.01)at the 6th day.With the increase of time in climate chambers,the activities of serum SOD,CAT decreased.NH_3and H_2S concentrations had the significant interaction effect on the activities of serum T-AOC(P<0.05),SOD(P<0.01),CAT(P<0.01),GSH-Px(P<0.01)at the2nd day,and the activities of serum SOD(P<0.01),CAT(P<0.01)at the 6th day.It is concluded that NH_3and H_2S could cause stress in goats and reduced the immune function and antioxidant capacity of goats.Furthermore,with the increase of NH_3and H_2S concentrations and the prolongation of the action time,the degree of damage to the goats became worse.
引文
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