白苦败痢散对靶动物猪的安全性试验
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摘要
为评价白苦败痢散对靶动物猪的安全性,选用4日龄健康三元杂交哺乳仔猪32只,随机分为4组,每组8只,按推荐的临床用药剂量设置白苦败痢散1倍剂量组(2 g/kg·bw)、3倍剂量组(6 g/kg·bw)、5倍剂量组(10 g/kg·bw)和对照组。3个用药组持续灌服给药7 d,每天1次,对照组灌服生理盐水。试验期间每天观察记录猪的精神状态、哺乳情况、粪便性状等表现,测定增重、血常规、血清生化指标、脏器指数并进行大体组织病理学观察。结果显示,按白苦败痢散推荐剂量的1、3、5倍剂量,持续灌服给药7 d,各观察组和测定指标与对照组相比均无显著性差异。说明白苦败痢散对猪是安全的。
In this work,we present a toxicity/safety evaluation experiment of Baikubailisan(veterinary herbal medicine) in piglets. The target animals,32 4-days-old triple-cross piglets,were randomly divided into four groups with dose level of 2 g/kg. bw(standard dose),6 g/kg. bw(triple dose),10 g/kg. bw(5 times dose),and equivalent normal saline(as control group) separately for once-daily dosing. During a 7-day observation,the daily health status,breastfeeding behavior,and fecal traits of each group were recorded. The hematology diagnosis,and visceral statistical and pathological analysis were performed. The analytical results showed no significant difference between the dose groups and the control group,indicating the safety of Baikubailisan utilization in piglets under the conditions in the experiment.
In this work,we present a toxicity/safety evaluation experiment of Baikubailisan(veterinary herbal medicine) in piglets. The target animals,32 4-days-old triple-cross piglets,were randomly divided into four groups with dose level of 2 g/kg. bw(standard dose),6 g/kg. bw(triple dose),10 g/kg. bw(5 times dose),and equivalent normal saline(as control group) separately for once-daily dosing. During a 7-day observation,the daily health status,breastfeeding behavior,and fecal traits of each group were recorded. The hematology diagnosis,and visceral statistical and pathological analysis were performed. The analytical results showed no significant difference between the dose groups and the control group,indicating the safety of Baikubailisan utilization in piglets under the conditions in the experiment.
引文
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[2]王瑞锋,王雪.白头翁汤的药理作用与临床应用[J].中医学报,2010,25(2):270-271.
[3]刘钟杰,许剑琴.中兽医学[M].北京:中国农业出版社,2002.
[4]夏方.中药地翁颗粒治疗仔猪白痢的有效性和安全性研究[D].长春:吉林大学,2015.
[5]董世山.中药对仔猪腹泻的防治作用及其机理研究[D].北京:中国农业科学院,2005.
[6]李冀.方剂学[M].北京:中国中医药出版社,2012:12.
[7]农业部兽药评估中心.兽药研究技术指导原则汇编[M].北京:化学工业出版社,2012.
[8]韩庆安,张志,李同山,等. 2010~2013年河北省猪场腹泻病流行病学调查[J].中国动物检疫,2015,32(5):5-8.
[9]田云,孙彦伟,王福广,等. 2012年广东猪群腹泻流行病学调查[J].广东畜牧兽医科技,2015,40(3):19-22.
[10]杨洪早,王东升,董书伟,等.仔猪腹泻的病因及中药防治研究进展[J].动物医学进展,2016,37(10):89-93.
[11]裔群英,丁立彤,徐凯,等.中草药防治仔猪腹泻的研究进展[J].农业与技术,2016,36(17):101-102.
[12]卢伟,尚丽元,王春凤,等.中草药在仔猪大肠杆菌性腹泻防治中的应用研究进展,黑龙江畜牧兽医,2016(07下):114-116.
[13]赖建彬,刘娟,朱兆荣,等.术苦芩颗粒对靶动物仔猪的安全性试验[J].中兽医医药杂志,2014(1):5-8.
[14]林烨,吕英军,张晓辉,等.替米考星肠溶颗粒对猪的安全性试验[J].中国兽药杂志,2016,50(10):22-31.