硫化氢/3-巯基丙酮酸转硫酶在癫痫患者颞叶中的表达
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摘要
目的探讨硫化氢(H_2S)/3-巯基丙酮酸转硫酶(3-MST)在癫痫患者颞叶中的表达。方法难治性癫痫患者(实验组)外侧颞叶标本15例,高颅压患者(对照组)内减压切除的正常外侧颞叶脑组织标本15例。检测外侧颞叶脑组织中H_2S含量,采用免疫荧光及Western blotting技术检测3-MST表达,观察H_2S/3-MST在癫痫患者外侧颞叶及高颅压患者正常外侧颞叶脑组织中的表达变化。结果实验组外侧颞叶脑组织细胞中H_2S/3-MST表达显著高于对照组,差异均具有统计学意义(均P<0.05)。结论在癫痫的病理生理过程中,H_2S/3-MST的高表达可能与其在癫痫中神经保护作用相关。
Objective To investigate the expression of hydrogen sulfide(H_2S)/3-mercaptopyruvate sulfurtransferase(3-MST) in temporal lobe of epileptic patients. Methods Fifteen cases of intractable epilepsy(experimental group) of lateral temporal lobe and 15 cases of normal lateral temporal lobe brain tissue excised by decompression of high intracranial pressure(control group).The content of H_2S in lateral temporal lobe brain tissues was detected. The expression of 3-MST was detected by immunofluorescence and Western blotting. The expression of H_2S/3-MST in normal lateral temporal lobe brain tissues of epileptic patients with lareral temporal lobe and intracranial hypertension was observed. Results The expression of H_2S/3-MST in the lateral temporal lobe brain tissue in the experimental group was significantly higher than that in the control group, and the difference was statistically significant(all P<0.05). Conclusion In the pathophysiological process of epilepsy, the high expression of H_2S/3-MST may be related to the neuroprotective effect of epilepsy in epilepsy.
Objective To investigate the expression of hydrogen sulfide(H_2S)/3-mercaptopyruvate sulfurtransferase(3-MST) in temporal lobe of epileptic patients. Methods Fifteen cases of intractable epilepsy(experimental group) of lateral temporal lobe and 15 cases of normal lateral temporal lobe brain tissue excised by decompression of high intracranial pressure(control group).The content of H_2S in lateral temporal lobe brain tissues was detected. The expression of 3-MST was detected by immunofluorescence and Western blotting. The expression of H_2S/3-MST in normal lateral temporal lobe brain tissues of epileptic patients with lareral temporal lobe and intracranial hypertension was observed. Results The expression of H_2S/3-MST in the lateral temporal lobe brain tissue in the experimental group was significantly higher than that in the control group, and the difference was statistically significant(all P<0.05). Conclusion In the pathophysiological process of epilepsy, the high expression of H_2S/3-MST may be related to the neuroprotective effect of epilepsy in epilepsy.
引文
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[2] Luo Y,Wu PF,Zhou J,et al.Aggravation of seizure-like events by Hydrogen sulfide:involvement of multiple targets that control neuronal excitability[J].CNS Neurosci Ther,2014,20(5):411-419.
[3] Asiyeh K,Forouzan A,layasadat K,et al.Sodium hydrogen sulfide (NaHS) ameliorates alterations caused by cisplatin in filtration slit diaphragm and podocyte cytoskeletal in rat kidney[J].J Nephropathol,2017,6(3):150-156.
[4] Kimura H.Physiological role of Hydrogen sulfide and polysulfide in the central nervous system[J].Neurochem Int,2013,63(5):492-497.
[5] Abe K,Kimura H.The possible role of Hydrogen sulfide as an endogenous neuromodulator[J].J Neurosci,1996,16(3):1066-1071.
[6] Lu M,Choo CH,Hu LF,et al.Hydrogen sulfide regulates intracellular pH in ratprimary cultured glia cells[J].Neurosci Res,2010,66(1):92-98.
[7] 代政伟,晏勇,张华,等.硫化氢与神经胶质细胞功能的研究进展[J].中国老年学杂志,2011,31(3):1072-1074.
[8] Sau Ha Cheung,James Yun Wong Lau.Hydrogen sulfide mediates athero-protection against oxidative stress via S-sulfhydration[J].PLoS One,2018,13(3):1-20.
[9] Oh GS,Pae HO,Lee BS,et al.Hydrogen sulfide inhibits nitric oxide production and nuclear factor-kappa B via hemeoxygenase-1 expression in RAW264.7 macrophages stimulated with lipopolysaccharide[J].Free Radic Biol Med,2006,41(1):106-119.
[10] Jung KK,Lee HS,Cho JY,et al.Inhibitory effect of curcumin on nitricoxide production from lipopolysaccharide-activated primary microglia[J].Life Sci,2006,79(21):2022-2031.
[11] Wei HJ,Xu JH,Li MH,et al.Hydrogen sulfide inhibits homocysteine-induced endoplasmic reticulum stress and neuronal apoptosis in rat hippocampus via upregulation of the BDNF-TrkB pathway[J].Acta Pharmacol Sin,2014,35(6):707-715.
[12] Cai WJ,Wang MJ,Moore PK,et al.The novel proangiogenic effect of Hydrogen sulfide is dependent on Akt phosphorylation[J].Cardiovasc Res,2007,76(1):29-40.
[13] Zhao H,Chan SJ,Ng YK,et al.Brain 3-Mercaptopyruvate sulfurtransferase (3MST):cellular localization and downregulation after acute stroke[J].PLoS One,2013,8(6):1-9.
[14] Agis-Torres A,Recio P,Elvira Lopez-Oliva MA,et al.Phosphodiesterase type 4 inhibition enhances nitric oxide- and Hydrogen sulfide-mediated bladder neck inhibitory neurotransmission[J].Sci Rep,2018,8(6):4711-4722.
[15] Tan BH,Wong PT,Bian JS.Hydrogen sulfide:a novel signaling molecule in the central nervous system[J].Neurochem Int,2010,56(1):3-10.
[16] 韩晓伟,秦伟,陈远寿,等.H2S/CBS体系在大鼠癫痫发病中的变化及意义[J].中国现代医学杂志,2012,22(26):13-16.
[17] Alves M,Beamer E,Engel T.The metabotropic purinergic P2Y receptor family as novel drug target in epilepsy[J].Front Pharmacol,2018,193(9):1-12.