摘要
目的研究蒺藜科植物蒺藜Tribulus terrester的干燥成熟果实的化学成分。方法利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、制备HPLC和薄层色谱等方法进行分离、纯化,并结合HR-ESI-MS与现代波谱学技术鉴定化合物结构。结果从蒺藜果实中分离得到14个化合物,分别鉴定为N-p-阿魏酰酪胺(1)、N-trans-p-coumaroyl-3-O-methyldopamine(2)、甲基阿魏酸(3)、咖啡酸甲酯(4)、原儿茶酸甲酯(5)、trifilines A(6)、5β-spirost-25(27)-en-3β-ol-12-one 3-O-{β-D-glucopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranoside}(7)、tribulosaponin B(8)、isoterrestrosin B(9)、25(R)-螺甾烷-3,5-二烯-12-酮(10)、25(R)-螺甾烷-24β-羟基-4-烯-3,12-酮(11)、26-O-β-D-glucopyranosyl-(25S)-5α-furostane-20(22)-en-12-one-3β,26-diol-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-galactopyranoside(12)、terrestrinone A1(13)和terrestrinone A2(14)。结论化合物1~7为首次从蒺藜科植物中分离得到。
Objective To study the chemical constituents from the fruits of Tribulus terrester. Methods The constituents were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography, preparative HPLC and TLC methods. The structures were identified by HR-ESI-MS and spectral analysis methods. Results Fourteen compounds were isolated from the fruits of Tribulus terrester and the structures were identified as N-p-feruloyltyramine(1), N-trans-p-coumaroyl-3-Omethyldopamine(2), methyl ferulic acid(3), caffeic acid methyl ester(4), protocatechuic acid methyl ester(5), trifilines A(6),5β-spirost-25(27)-en-3β-ol-12-one 3-O-{β-D-glucopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranoside}(7),tribulosaponin B(8), isoterrestrosin B(9), 25(R)-spirostane-3,5-diene-12-one(10), 25(R)-spirostane-24β-ol-4-ene-3,12-dione(11),26-O-β-D-glucopyranosyl-(25 S)-5α-furostane-20(22)-en-12-one-3β,26-diol-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-galactopyranoside(12), terrestrinone A1(13), and terrestrinone A2(14). Conclusion Compounds 1—7 are obtained from the Zygophllyaceae for the first time.
引文
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