硝酸钙胁迫对黄瓜幼苗生长和生理特性的影响
|
摘要
以温室专用黄瓜品种"津春2号"和"津春4号"为试材,采用营养液水培法,研究了不同浓度Ca(NO_3)_2胁迫对营养液水培黄瓜幼苗生长、膜脂过氧化和有机渗调物质含量的影响,以期为耐盐黄瓜新品种选育及设施黄瓜抗盐栽培提供参考。结果表明:随Ca(NO_3)_2胁迫浓度提高,黄瓜幼苗的株高、茎粗、根长、叶片数、最大叶长、最大叶宽及植株干质量、鲜质量和含水量均不同程度降低,光合色素和可溶性蛋白质含量呈"增加-降低"的规律,质膜透性、丙二醛和脯氨酸含量显著增加。说明Ca(NO_3)_2胁迫通过渗透胁迫造成了细胞水分亏缺,并通过氧化胁迫破坏了细胞膜结构,严重抑制了黄瓜幼苗的生长,但供试的2个品种对Ca(NO_3)_2胁迫的耐性并没有明显差异。
The effects of different Ca(NO_3)_2 concentrations on the growth,lipid peroxidation and the contents of osmotic substances of cucumber seedlings in nutrient solution were studied with greenhouse dedicated cucumber‘Jinchun No.2'and‘Jinchun No.4',to provide reference for breeding of salt tolerant cucumber and salt tolerant cultivating in greenhouse.The results showed that the plant height,stem diameter,root length,leaf number,leaf length,leaf width,plant fresh mass,plant dry mass and water content of cucumber seedlings were decreased with increasing Ca(NO_3)_2 concentration.The contents of photosynthetic pigments and soluble protein were increased at lower concentrations Ca(NO_3)_2 ,and decreased at higher concentrations Ca(NO_3)_2 .The membrane permeability,the contents of MDA and proline were increased significantly with increasing Ca(NO_3)_2 concentration.The study illustrated that Ca(NO_3)_2 stress seriously inhibited the growth of cucumber seedlings mainly through cell water deficit caused by osmotic stress,and cell membrane destruction caused by lipid peroxidation,but there was no apparent difference in the tolerance to Ca(NO_3)_2 stress between the two cultivars.
The effects of different Ca(NO_3)_2 concentrations on the growth,lipid peroxidation and the contents of osmotic substances of cucumber seedlings in nutrient solution were studied with greenhouse dedicated cucumber‘Jinchun No.2'and‘Jinchun No.4',to provide reference for breeding of salt tolerant cucumber and salt tolerant cultivating in greenhouse.The results showed that the plant height,stem diameter,root length,leaf number,leaf length,leaf width,plant fresh mass,plant dry mass and water content of cucumber seedlings were decreased with increasing Ca(NO_3)_2 concentration.The contents of photosynthetic pigments and soluble protein were increased at lower concentrations Ca(NO_3)_2 ,and decreased at higher concentrations Ca(NO_3)_2 .The membrane permeability,the contents of MDA and proline were increased significantly with increasing Ca(NO_3)_2 concentration.The study illustrated that Ca(NO_3)_2 stress seriously inhibited the growth of cucumber seedlings mainly through cell water deficit caused by osmotic stress,and cell membrane destruction caused by lipid peroxidation,but there was no apparent difference in the tolerance to Ca(NO_3)_2 stress between the two cultivars.
引文
[1]张真和,马兆红.我国设施蔬菜产业概况与“十三五”发展重点[J].中国蔬菜,2017(5):1-5.
[2]张志斌.国外设施园艺的发展与启示[J].江苏农业学报,2012,28(4):861-866.
[3]杨春霞,张艳,李彩虹,等.宁夏设施土壤盐分离子组成及含量变化特点[J].西北农业学报,2014,23(1):201-206.
[4]张古文,朱月林,刘正鲁,等.Ca(NO3)2胁迫对嫁接番茄生长、抗氧化酶活性和活性氧代谢的影响[J].植物营养与肥料学报,2008,14(3):527-532.
[5] COLLA G,ROUPHAEL Y,REA E,et al.Grafting cucumber plants enhance tolerance to sodium chloride and sulfate salinization[J].Scientia Horticulturae,2012,135:177-185.
[6]江绪文,李贺勤,王建华.盐胁迫下黄芩种子萌发及幼苗对外源抗坏血酸的生理响应[J].植物生理学报,2015,51(2):166-170.
[7] PARIDA A K,DAS A B.Salt tolerance and salinity effects on plants:A review[J].Ecotoxicology and Environmental Safety,2005,60(3):324-349.
[8] HU X H,XU Z R,XU W N,et al.Application ofγ-aminobutyric acid demonstrates a protective role of polyamine and GABA metabolism in muskmelon seedlings under Ca(NO3)2stress[J].Plant Physiology and Biochemistry,2015,92:1-10.
[9]刘志媛,朱祝军,钱亚榕,等.等渗Ca(NO3)2和NaCl对番茄幼苗生长的影响[J].园艺学报,2001,28(1):31-35.
[10]张振贤.蔬菜栽培学[M].北京:中国农业大学出版社,2008:139-166.
[11]DUAN J J,LI J,GUO S R,et al.Exogenous spermidine affects polymine metabolism in salinity-stressed Cucumis sativus roots and enhances short-term salinity tolerance[J].Journal of Plant Physiology,2008,165:1620-1635.
[12]沈伟其.测定水稻叶片叶绿素含量的混合液提取法[J].植物生理学通讯,1988(3):62-64.
[13]王素平,郭世荣,胡晓辉,等.盐胁迫对黄瓜幼苗叶片光合色素含量的影响[J].江西农业大学学报,2006,28(1):32-38.
[14]RAO K V M,SRESTY T V S.Antioxidative parameters in the seedlings of pigeonpea(Cajanus cajan L.Mill spaugh)in response to Zn and Ni stresses[J].Plant Science,2000,157(1):113-128.
[15]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:208-209.
[16]蔡庆生.植物生理学实验[M].北京:中国农业大学出版社,2013:175-177.
[17]ZHU J K.Plant salt tolerance[J].Trends in Plant Science,2001,6(2):66-71.
[18]魏国强,朱祝军,方学智,等.NaCl胁迫对不同品种黄瓜幼苗生长、叶绿素荧光特性和活性氧代谢的影响[J].中国农业科学,2004,37(11):1754-1759.
[19]张立军,刘新.植物生理学[M].2版.北京:科学出版社,2011:97-103.
[20]韩志平,郭世荣,焦彦生,等.NaCl胁迫对西瓜幼苗生长和光合气体交换参数的影响[J].西北植物学报,2008,28(4):745-751.
[21]ALLAKHVERDIEV S I,SAKAMOTO A,NISHIYAMA Y,et al.Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.[J].Plant Physiology,2000,123(3):1047-1056.
[22]徐玉伟,郭世荣,程玉静,等.Ca(NO3)2对盐胁迫下黄瓜幼苗生长及膜质过氧化的影响[J].中国蔬菜,2010(4):14-18.
[23]PARVAIZ A,SATYAWATI S.Salt stress and phyto-biochemical response of plants:A review[J].Plant Soil Environnment,2008,54(3):89-99.
[24]朱玉鹏,孟祥浩,盖伟玲,等.盐胁迫对冬小麦花后抗氧化酶、渗透调节物质的影响[J].中国农学通报,2017,33(19):1-6.
[25]陈晓亚,汤章城.植物生理与分子生物学[M].3版.北京:高等教育出版社,2007:533-551.
[26]ASHRAF M,FOOLAD M R.Role of glycine betaine and proline in improving plant abiotic stress resistance[J].Environmental and Experimental Botany,2007,59:206-216.
[27]陈阳春,张本厚,贾明良,等.盐胁迫对半夏组培苗生长及生理指标的影响[J].江苏农业科学,2014,42(12):62-66.
[28]赵勇,马雅琴,翁跃进.盐胁迫下小麦甜菜碱和脯氨酸含量的变化[J].植物生理与分子生物学报,2005,31(11):103-106.
[29]韩志平,郭晓东,张海霞,等.硝酸钙胁迫对黄瓜种子萌发特性的影响[J].山西农业科学,2013,41(11):1186-1189.
[2]张志斌.国外设施园艺的发展与启示[J].江苏农业学报,2012,28(4):861-866.
[3]杨春霞,张艳,李彩虹,等.宁夏设施土壤盐分离子组成及含量变化特点[J].西北农业学报,2014,23(1):201-206.
[4]张古文,朱月林,刘正鲁,等.Ca(NO3)2胁迫对嫁接番茄生长、抗氧化酶活性和活性氧代谢的影响[J].植物营养与肥料学报,2008,14(3):527-532.
[5] COLLA G,ROUPHAEL Y,REA E,et al.Grafting cucumber plants enhance tolerance to sodium chloride and sulfate salinization[J].Scientia Horticulturae,2012,135:177-185.
[6]江绪文,李贺勤,王建华.盐胁迫下黄芩种子萌发及幼苗对外源抗坏血酸的生理响应[J].植物生理学报,2015,51(2):166-170.
[7] PARIDA A K,DAS A B.Salt tolerance and salinity effects on plants:A review[J].Ecotoxicology and Environmental Safety,2005,60(3):324-349.
[8] HU X H,XU Z R,XU W N,et al.Application ofγ-aminobutyric acid demonstrates a protective role of polyamine and GABA metabolism in muskmelon seedlings under Ca(NO3)2stress[J].Plant Physiology and Biochemistry,2015,92:1-10.
[9]刘志媛,朱祝军,钱亚榕,等.等渗Ca(NO3)2和NaCl对番茄幼苗生长的影响[J].园艺学报,2001,28(1):31-35.
[10]张振贤.蔬菜栽培学[M].北京:中国农业大学出版社,2008:139-166.
[11]DUAN J J,LI J,GUO S R,et al.Exogenous spermidine affects polymine metabolism in salinity-stressed Cucumis sativus roots and enhances short-term salinity tolerance[J].Journal of Plant Physiology,2008,165:1620-1635.
[12]沈伟其.测定水稻叶片叶绿素含量的混合液提取法[J].植物生理学通讯,1988(3):62-64.
[13]王素平,郭世荣,胡晓辉,等.盐胁迫对黄瓜幼苗叶片光合色素含量的影响[J].江西农业大学学报,2006,28(1):32-38.
[14]RAO K V M,SRESTY T V S.Antioxidative parameters in the seedlings of pigeonpea(Cajanus cajan L.Mill spaugh)in response to Zn and Ni stresses[J].Plant Science,2000,157(1):113-128.
[15]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:208-209.
[16]蔡庆生.植物生理学实验[M].北京:中国农业大学出版社,2013:175-177.
[17]ZHU J K.Plant salt tolerance[J].Trends in Plant Science,2001,6(2):66-71.
[18]魏国强,朱祝军,方学智,等.NaCl胁迫对不同品种黄瓜幼苗生长、叶绿素荧光特性和活性氧代谢的影响[J].中国农业科学,2004,37(11):1754-1759.
[19]张立军,刘新.植物生理学[M].2版.北京:科学出版社,2011:97-103.
[20]韩志平,郭世荣,焦彦生,等.NaCl胁迫对西瓜幼苗生长和光合气体交换参数的影响[J].西北植物学报,2008,28(4):745-751.
[21]ALLAKHVERDIEV S I,SAKAMOTO A,NISHIYAMA Y,et al.Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.[J].Plant Physiology,2000,123(3):1047-1056.
[22]徐玉伟,郭世荣,程玉静,等.Ca(NO3)2对盐胁迫下黄瓜幼苗生长及膜质过氧化的影响[J].中国蔬菜,2010(4):14-18.
[23]PARVAIZ A,SATYAWATI S.Salt stress and phyto-biochemical response of plants:A review[J].Plant Soil Environnment,2008,54(3):89-99.
[24]朱玉鹏,孟祥浩,盖伟玲,等.盐胁迫对冬小麦花后抗氧化酶、渗透调节物质的影响[J].中国农学通报,2017,33(19):1-6.
[25]陈晓亚,汤章城.植物生理与分子生物学[M].3版.北京:高等教育出版社,2007:533-551.
[26]ASHRAF M,FOOLAD M R.Role of glycine betaine and proline in improving plant abiotic stress resistance[J].Environmental and Experimental Botany,2007,59:206-216.
[27]陈阳春,张本厚,贾明良,等.盐胁迫对半夏组培苗生长及生理指标的影响[J].江苏农业科学,2014,42(12):62-66.
[28]赵勇,马雅琴,翁跃进.盐胁迫下小麦甜菜碱和脯氨酸含量的变化[J].植物生理与分子生物学报,2005,31(11):103-106.
[29]韩志平,郭晓东,张海霞,等.硝酸钙胁迫对黄瓜种子萌发特性的影响[J].山西农业科学,2013,41(11):1186-1189.