响应面法优选银槐解毒颗粒提取工艺
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摘要
目的优选银槐解毒颗粒提取工艺。方法在单因素试验基础上,采用响应面法考察提取时间、料液比、浸泡时间对银槐解毒颗粒中绿原酸、总黄酮提取方法的影响。采用HPLC同时测定绿原酸、总黄酮的含量,流动相为乙腈(A)-0.1%磷酸水(B),梯度洗脱(0~20 min,13%A;20~30 min,13%~18%A;30~55 min,18%~20%A;55~65 min,20%~23%A),绿原酸和黄酮类成分的检测波长分别为327、256 nm。结果最佳提取工艺条件为浸泡时间60 min,加12倍量水,提取130 min,提取2次。绿原酸、芦丁、黄芩苷平均浓度分别为51.497、68.872、25.763μg/g,平均浸膏得率为33.105%。结论优化后的提取工艺稳定可行,可为银槐解毒颗粒后续生产提供数据支持。
Objective To optimize extraction process of Yinhuai Jiedu Granula. Methods On the basis of single factor experiments, effects of soaking time, solvent dosage and extracting time on contents of chlorogenic acid and total flavonoids were investigated by response surface methodology. HPLC was used to determine contents of chlorogenie acid and total flavonoids with mobile phase of acetonitrile-0.1% phosphoric acid for gradient elution(0–20 min, 13%A; 20–30 min, 13%–18%A; 30–55 min, 18%–20%A; 55–65 min, 20%–23%A) and detection wavelengthes of 327 nm and 256 nm for chlorogenic acid and total flavonoids respectively. Results Optimal extraction process was as following: soaked 60 minutes and extracted twice with 12 times the amount of water per time of 130 minutes. Concentrations of chlorogenic acid, rutin and baicalin were 51.497, 68.872, 25.763 μg/g respectively. The average extract yield was 33.105%. Conclusion The optimized extraction process is feasible and stable, which can provide data support for the further research on Yinhuaijiedu granula.
Objective To optimize extraction process of Yinhuai Jiedu Granula. Methods On the basis of single factor experiments, effects of soaking time, solvent dosage and extracting time on contents of chlorogenic acid and total flavonoids were investigated by response surface methodology. HPLC was used to determine contents of chlorogenie acid and total flavonoids with mobile phase of acetonitrile-0.1% phosphoric acid for gradient elution(0–20 min, 13%A; 20–30 min, 13%–18%A; 30–55 min, 18%–20%A; 55–65 min, 20%–23%A) and detection wavelengthes of 327 nm and 256 nm for chlorogenic acid and total flavonoids respectively. Results Optimal extraction process was as following: soaked 60 minutes and extracted twice with 12 times the amount of water per time of 130 minutes. Concentrations of chlorogenic acid, rutin and baicalin were 51.497, 68.872, 25.763 μg/g respectively. The average extract yield was 33.105%. Conclusion The optimized extraction process is feasible and stable, which can provide data support for the further research on Yinhuaijiedu granula.
引文
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[3]王远,陈良,刘志雯,等.响应面法优选清喉利咽口含片的提取工艺[J].中国实验方剂学杂志,2016,22(4):20-23.
[4]靳倩,申凌娜,李淑芳,等.槐花药材中芦丁、槲皮素、染料木素的含量测定[J].中医学报,2015,30(8):1176-1178.
[5]张俊鹏,张宝婵,李瑞明,等.HPLC法检测不同地区野生槐花花蕾中芦丁与槲皮素[J].中成药,2014,36(12):2573-2576.
[6]成林,刘吉成,卢森华,等.HPLC测定小槐花药材中山柰酚的含量[J].中国实验方剂学杂志,2013,19(15):92-94.
[7]吴虹霁,兰昌云,陈媛.槐花的研究进展[J].广东微量元素科学,2006,13(6):1-6.
[8]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:301.
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[10]王孟华,曲玮,梁敬钰.黄芩的研究进展[J].海峡药学,2013,25(9):6-13.