鸭肉中金霉素残留量LSSVR-同步荧光法测定
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摘要
为实现鸭肉中金霉素残留含量的快速测定,提出了应用同步荧光法结合最小二乘支持向量回归(LSSVR)来建立鸭肉中金霉素残留含量的定量分析模型。首先应用三维同步荧光法对波长250～450 nm的同步荧光光谱进行分析,确定检测鸭肉中的金霉素含量的最佳波长差Δλ为70 nm;然后使用sym8小波的2层分解对原始同步荧光光谱进行光谱预处理,对预处理后的光谱采用遗传算法(GA)结合交互验证均方根误差(RMSECV)方法选择了16个波长作为检测模型的输入特征向量;最后对LSSVR、BP和SVM_SteveGunn 3种模型进行性能比较,以LSSVR模型的预测效果最好,其预测集的决定系数R2和预测均方根误差分别为0.9491和2.5660。试验结果表明,同步荧光法结合LSSVR模型检测鸭肉中的金霉素残留含量是可行的。
In order to achieve the rapid prediction of aureomycin contents in duck meat,quantitative analysis models of aureomycin contents in duck meat were established by using synchronous fluorescence spectroscopy and LSSVR method.Firstly,synchronous fluorescence spectroscopies between 250 nm and 450 nm were analyzed by using 3-D synchronous fluorescence spectroscopy,and 70 nm was selected as the optimum wavelength difference.Secondly,the sym8 wavelet with two levels decomposition was used to complete the pretreatment of the original synchronous fluorescence spectroscopy,and 16 characteristic wavelengths were selected as the inputs of detection model combining with genetic algorithm and the root mean square error of cross-validation.Lastly,the performances of three models of LSSVR,BP and SVM_SteveGunn were compared.LSSVR model was optimal among them,and the determination coefficient R2 and the root mean squared error of prediction samples were 0.949 1 and 2.566 0,respectively.The experimental results showed that it was feasible to predict the aureomycin contents in duck meat combining with synchronous fluorescence spectroscopy and LSSVR.
In order to achieve the rapid prediction of aureomycin contents in duck meat,quantitative analysis models of aureomycin contents in duck meat were established by using synchronous fluorescence spectroscopy and LSSVR method.Firstly,synchronous fluorescence spectroscopies between 250 nm and 450 nm were analyzed by using 3-D synchronous fluorescence spectroscopy,and 70 nm was selected as the optimum wavelength difference.Secondly,the sym8 wavelet with two levels decomposition was used to complete the pretreatment of the original synchronous fluorescence spectroscopy,and 16 characteristic wavelengths were selected as the inputs of detection model combining with genetic algorithm and the root mean square error of cross-validation.Lastly,the performances of three models of LSSVR,BP and SVM_SteveGunn were compared.LSSVR model was optimal among them,and the determination coefficient R2 and the root mean squared error of prediction samples were 0.949 1 and 2.566 0,respectively.The experimental results showed that it was feasible to predict the aureomycin contents in duck meat combining with synchronous fluorescence spectroscopy and LSSVR.
引文
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14周竹,李小昱,高海龙,等.马铃薯干物质含量高光谱检测中变量选择方法比较[J].农业机械学报,2012,43(2):128～133.Zhou Zhu,Li Xiaoyu,Gao Hailong,et al.Comparison of different variable selection methods on potato dry matter detectionby hyperspectral imaging technology[J].Transactions of the Chinese Society for Agricultural Machinery,2012,43(2):128～133.(in Chinese)
15冯晓莉,仇宝云,杨兴丽,等.大型泵站运行优化方法及其应用[J].排灌机械工程学报,2011,29(2):127～132.Feng Xiaoli,Qiu Baoyun,Yang Xingli,et al.Optimal methods and its application of large pumping station operation[J].Journal of Drainage and Irrigation Machinery Eegineering,2011,29(2):127～132.(in Chinese)
2 Huet A C,Charlier C,Tittlemier S A,et al.Simultaneous determination of(Fluoro)quinolone antibiotics in kidney,marineproducts,eggs,and muscle by enzyme-linked immunosorbent assay(ELISA)[J].Journal of Agricultural and FoodChemistry,2006,54(8):2 822～2 827.
3 Shen H Y,Jiang H L.Screening,determination and confirmation of chloramphenicol in seafood,meat and honey usingELISA,HPLC-LJVD,GC-ECD,GC-MS-EI-SIM and GCMS-NCI-SIM methods[J].Analytica Chimica Acta,2005,535(1～2):33～41.
4 Ang C Y W,Luo W H,Kiessling C R,et al.A bridging study between liquid chromatography and microbial inhibition assaymethod for determining amoxicillin residues in catfish muscle[J].Journal of AOAC International,1998,81(1):33～39.
5 Pikkemaat M G,Dijk S O,Schouten J,et al.A new microbial screening method for the detection of antimicrobial residues inslaughter animals:the nouws antibiotic test(NAT-screening)[J].Food Control,2008,19(8):781～789.
6 Mohammad M K,Sang H L,Hyun S L,et al.A batch chemiluminescence determination of enoxacin using a tris-(1,10-phenanthroline)ruthenium(II)—cerium(IV)system[J].Journal of Fluorescence,2006,16(4):535～540.
7 Pikkemaat M G.Microbial screening methods for detection of antibiotic residues in slaughter animals[J].Analytical andBioaralytical Chemistry,2009,395(4):893～905.
8葛立新,高淑梅,刘思斌.基于紫外光诱导血浆的三维同步荧光光谱共振能量转移及能量再吸收的分析[J].光子学报,2011,40(10):1 500～1 504.Ge Lixin,Gao Shumei,Liu Sibin.Three-dimensional UV-induced plasma synchronous fluorescence spectrum resonance energytransfer and energy analysis of resorption[J].Acta Photonica Sinica,2011,40(10):1 500～1 504.(in Chinese)
9王春艳,江华鸿,高居伟,等.基于三维同步荧光光谱确定原油样品浓度的新方法[J].光谱学与光谱分析,2006,26(6):1 080～1 083.Wang Chunyan,Jiang Huahong,Gao Juwei,et al.A new method for concentration determination of crude oil sample usingthree-dimensional synchronous fluorescence spectra[J].Spectroscopy and Spectral Analysis,2006,26(6):1 080～1 083.(in Chinese)
10侯法菊,王玉宝,苗延虹,等.荧光光度法研究修饰β-环糊精与Eu(Ⅲ)-金霉素体系的相互作用及其应用[J].分析科学学报,2011,27(2):231～234.Hou Faju,Wang Yubao,Miao Yanhong,et al.Fluorescence study of the interaction between the modifiedβ-cyclodextrinand europium(Ⅲ)-chlortetracycline system and its analytical application[J].Journal of Analytical Science,2011,27(2):231～234.(in Chinese)
11陈志敏,陈冠华,郝庆红,等.导数同步荧光法测定猪肉中强力霉素残留[J].河北大学学报:自然科学版,2011,31(1):57～62.Chen Zhimin,Chen Guanhua,Hao Qinghong,et al.Determination of doxycycline residue in pork by derivative synchronousfluorimetry[J].Journal of Hebei University:Natural Science Edition,2011,31(1):57～62.(in Chinese)
12张艳,陈冠华,郝庆红,等.硫酸氧化同步荧光法测定猪肉中的螺旋霉素残留[J].河北大学学报:自然科学版,2010,30(1):64～68.Zhang Yan,Chen Guanhua,Hao Qinghong,et al.Determination of spiramycin residue in pork by synchronous fluorimetrywith sulphuric acid oxidation[J].Journal of Hebei University:Natural Science Edition,2010,30(1):64～68.(in Chinese)
13蔡剑华,王先春,胡惟文.基于EMD的土壤有机质含量近红外光谱检测[J].农业机械学报,2010,41(9):182～186.Cai Jianhua,Wang Xianchun,Hu Weiwen.Near-infrared spectrum detection of soil organic matter content based onempirical mode decomposition[J].Transactions of the Chinese Society for Agricultural Machinery,2010,41(9):182～186.(in Chinese)
14周竹,李小昱,高海龙,等.马铃薯干物质含量高光谱检测中变量选择方法比较[J].农业机械学报,2012,43(2):128～133.Zhou Zhu,Li Xiaoyu,Gao Hailong,et al.Comparison of different variable selection methods on potato dry matter detectionby hyperspectral imaging technology[J].Transactions of the Chinese Society for Agricultural Machinery,2012,43(2):128～133.(in Chinese)
15冯晓莉,仇宝云,杨兴丽,等.大型泵站运行优化方法及其应用[J].排灌机械工程学报,2011,29(2):127～132.Feng Xiaoli,Qiu Baoyun,Yang Xingli,et al.Optimal methods and its application of large pumping station operation[J].Journal of Drainage and Irrigation Machinery Eegineering,2011,29(2):127～132.(in Chinese)