滇牡丹红斑病最适诱抗剂的筛选试验
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摘要
研究植物病害诱抗剂对滇牡丹红斑病的诱抗效应,以期为红斑病的防治提供一种新的途径。采用康壮素(Harpin)、苯并噻二唑(BTH)和水杨酸(SA)3种常见诱抗剂对滇牡丹红斑病进行了诱抗试验,分析了其发病指数、诱抗效果、生理等相关指标变化。结果表明:3种诱抗剂对滇牡丹红斑病诱抗效果显著,发病指数均显著下降,其中以BTH的诱抗效果最佳;并确定BTH的最佳使用浓度为100 mg/L,诱抗效果高达68.27%。在此浓度处理后,叶片病斑面积停止增长,边缘逐渐愈合,SAFR和MDA含量显著降低,可溶性蛋白、色素和相关酶系活力指标均显著增加,这些变化与流胶病的抗性变化密切相关。3种诱抗剂对滇牡丹红斑病原菌不产生毒杀作用,而是诱导植物系统获得性抗性(SAR),其作用机理有待进一步的研究。
In order to provide a new way to preventing the Cladosporium red spot,it was significant to study of the effect of plant disease inducers on resistance induction against red spot of Paeonia delavayi. 3 kinds of plant disease inducers that include Harpin,BTH and SA were been resistance test to red spot and the index of infection index,induced-resistance effects and related physiological and enzyme activity indexes were determined. The results showed that the 3 kinds of inducers had significant induced-resistance effects on the red spot and the infection index was dramatic decline. The optimal inducer was BTH and the best concentration was 100 mg/L that induced-resistance effect to the red spot was to be 68. 27%. The indexes of SAFR and MDA were dramatic decline,but the indexes of soluble protein,Pigment and related enzymes activity were obviously higher than those control processing,what were closely related with resistance induction to the red spot. Three kinds of inducers hadn' t toxic activity to the Cladosporium paeoniae and could lead P. delavayi to produce systemic acquired resistance( SAR) effectively,which mechanism would have to be further studied.
In order to provide a new way to preventing the Cladosporium red spot,it was significant to study of the effect of plant disease inducers on resistance induction against red spot of Paeonia delavayi. 3 kinds of plant disease inducers that include Harpin,BTH and SA were been resistance test to red spot and the index of infection index,induced-resistance effects and related physiological and enzyme activity indexes were determined. The results showed that the 3 kinds of inducers had significant induced-resistance effects on the red spot and the infection index was dramatic decline. The optimal inducer was BTH and the best concentration was 100 mg/L that induced-resistance effect to the red spot was to be 68. 27%. The indexes of SAFR and MDA were dramatic decline,but the indexes of soluble protein,Pigment and related enzymes activity were obviously higher than those control processing,what were closely related with resistance induction to the red spot. Three kinds of inducers hadn' t toxic activity to the Cladosporium paeoniae and could lead P. delavayi to produce systemic acquired resistance( SAR) effectively,which mechanism would have to be further studied.
引文
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[12]岳子石,宋晓斌,王方,等.3种抗性诱导剂诱导枣树抗缩果病的研究[J].西北林学院学报,2016,31(1):153-157.
[13]汪尚,徐鹭芹,张亚仙,等.水杨酸介导植物抗病的研究进展[J].植物生理学报,2016,52(5):581-590.
[14]侯珲,朱建兰,周红平,等.BTH和水杨酸(SA)对甜瓜抗白粉病的诱导作用[J].果树学报,2006,23(5):736-739.
[15]王文娟,赵建庄,魏朝俊,等.苯并噻二唑(BTH)对苹果抗斑点落叶病的诱导[J].果树学报,2008,25(3):362-366.
[2]刘德晶,焦晓旭.油用牡丹产业发展现状及对策[J].西部林业科学,2015,44(2):170-173.
[3]吴玉柱,季延平,刘慇,等.牡丹的主要病害及其防治研究[J].西部林业科学,2006,35(4):40-44.
[4]Gilchrist L,Galdames R,Chahin G,et al.First report in Chile of Mycocentrospora acerina,causal agent of Peony(Paeonia lactiflora)red spot[J].Plant Disease,2015,99(2):284-284.
[5]石良红,李玲,申宏伟,等.牡丹红斑病病原鉴定与ITS序列分析[J].农学学报,2014,4(10):32-35.
[6]杨德翠.牡丹红斑病抗性鉴定方法研究[J].江苏农业科学,2016,44(1):168-170.
[7]赵继红,孙淑君,李建中.植物诱导抗病性与诱抗剂研究进展[J].植物保护,2003,29(4):7-10.
[8]Endo J,Takahashi W,Ikegami T,et al.Induction of flowering by inducers of systemic acquired resistance in the plant[J].Bioscience Biotechnology&Biochemistry,2014,73(1):183-185.
[9]范志金,刘秀峰,刘凤丽,等.植物抗病激活剂诱导植物抗病性的研究进展[J].植物保护学报,2005,32(1):28-30.
[10]Faoro F,Gozzo F.Is modulating virus virulence by induced systemic resistance realistic[J].Plant Science,2015,234:1-13.
[11]王涛,陈泽鹏,万树青,等.苯并噻二唑(BTH)诱导烟草抗青枯病活性与抗病机理研究[J].中国烟草学报,2008,14(S1):29-32.
[12]岳子石,宋晓斌,王方,等.3种抗性诱导剂诱导枣树抗缩果病的研究[J].西北林学院学报,2016,31(1):153-157.
[13]汪尚,徐鹭芹,张亚仙,等.水杨酸介导植物抗病的研究进展[J].植物生理学报,2016,52(5):581-590.
[14]侯珲,朱建兰,周红平,等.BTH和水杨酸(SA)对甜瓜抗白粉病的诱导作用[J].果树学报,2006,23(5):736-739.
[15]王文娟,赵建庄,魏朝俊,等.苯并噻二唑(BTH)对苹果抗斑点落叶病的诱导[J].果树学报,2008,25(3):362-366.