3种诱抗剂对凤丹抗病害的诱导效果
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摘要
分别用5个浓度的3种诱抗剂(Harpin、BTH和SA)喷施凤丹叶面,并以清水处理为对照,统计叶片病斑面积和病叶数,从而测定诱抗剂对凤丹抗3种主要病害(红斑病、灰霉病和黑斑病)的诱导效果;同时,测定了最适浓度诱抗剂对3种病原菌的抑制活性,以及对凤丹植株生理指标的影响.结果表明:3种诱抗剂均可有效地诱导凤丹对不同病害产生抗性,其中,BTH的诱抗效果最佳;3种诱抗剂对凤丹抗主要病害的诱导效果均表现为红斑病>黑斑病>灰霉病;诱抗剂处理显著提高了凤丹植株可溶性蛋白和色素含量及相关酶活性,显著降低了超氧阴离子自由基和丙二醛的含量;诱抗剂对凤丹3种病害的病原菌无毒杀作用,但可诱导植株获得系统抗性.
Three inducers(Harpin,BTH and SA) were sprayed to Paeonia ostii in 5 concentrations,with water spray being the control,total spot area and the number of infected leaves were recorded. Meanwhile,at the optimal concentration of inducers,plaque diameter of Cladosporium paeoniae,Botrytis paeoniae and Alternaria alternate were compared,key biochemical indicators related to infections were tested. The results showed that all 3 inducers had significant induction effect on P.ostii resistance to pathogenic fungi,with BTH being the optimal inducer. Averagely induction effect of resistance to fungi in descending order was C.paeoniae,A.alternata,and B.paeoniae. Superoxide anion free radical and malondialdehyde levels declined dramatically while levels of soluble protein and pigments,and enzymes activity increased compared with the control. In additon,three inducers were not capable of killing pathogens,however,they can stimulate systemic acquired resistance of plant.
Three inducers(Harpin,BTH and SA) were sprayed to Paeonia ostii in 5 concentrations,with water spray being the control,total spot area and the number of infected leaves were recorded. Meanwhile,at the optimal concentration of inducers,plaque diameter of Cladosporium paeoniae,Botrytis paeoniae and Alternaria alternate were compared,key biochemical indicators related to infections were tested. The results showed that all 3 inducers had significant induction effect on P.ostii resistance to pathogenic fungi,with BTH being the optimal inducer. Averagely induction effect of resistance to fungi in descending order was C.paeoniae,A.alternata,and B.paeoniae. Superoxide anion free radical and malondialdehyde levels declined dramatically while levels of soluble protein and pigments,and enzymes activity increased compared with the control. In additon,three inducers were not capable of killing pathogens,however,they can stimulate systemic acquired resistance of plant.
引文
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[15]侯珲,朱建兰,周红平,等.BTH和水杨酸(SA)对甜瓜抗白粉病的诱导作用[J].果树学报,2006,23(5):736-739.
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[18]王文娟,赵建庄,魏朝俊,等.苯并噻二唑(BTH)对苹果抗斑点落叶病的诱导[J].果树学报,2008,25(3):362-366.
[2]刘德晶,焦晓旭.油用牡丹产业发展现状及对策[J].西部林业科学,2015,44(2):170-173.
[3]高洁,罗建勋,许戈,等.四川高含油量牡丹品种“丹凤1号”良种选育[J].西部林业科学,2016,45(4):114-118.
[4]吴玉柱,季延平,刘慇,等.牡丹的主要病害及其防治研究[J].西部林业科学,2006,35(4):40-44.
[5]GILCHRIST L,GALDAMES R,CHAHIN G,et al.First report in Chile of Mycocentrospora acerina,causal agent of peony(Paeonia lactiflora)red spot[J].Plant Disease,2015,99(2):284.
[6]石良红,李玲,申宏伟,等.牡丹红斑病病原鉴定与ITS序列分析[J].农学学报,2014,4(10):32-35.
[7]何童童,董梦琳,侯小改,等.牡丹病害防治方法研究进展[J].陕西林业科学,2014(6):72-75,79.
[8]ENDO J,TAKAHASHI W,IKEGAMI T,et al.Induction of flowering by inducers of systemic acquired resistance in the plant[J].Bioscience Biotechnology&Biochemistry,2014,73(1):183-185.
[9]邱德文.植物免疫诱抗剂的研究进展与应用前景[J].中国农业科技导报,2014,16(1):39-45.
[10]FAORO F,GOAAO F.Is modulating virus virulence by induced systemic resistance realistic[J].Plant Science,2015,234:1-13.
[11]李芳,陈建明,陈忠其,等.植物免疫诱抗剂对番茄灰霉病和早疫病的抑菌效果[J].农药,2016,55(3):214-216.
[12]左应梅,简邦丽,黄正鸿,等.4种诱抗剂对三七生长、抗病性及相关酶活性的影响[J].中国现代中药,2016,18(8):1 024-1 029.
[13]李盼盼,丁伟,刘秋萍,等.硅和苯并噻二唑诱导烟草抗青枯病的机理分析[J].烟草科技,2016,49(7):23-30.
[14]石忠强,罗珺,缪福俊,等.樱桃流胶病最适诱抗剂的筛选试验[J].西部林业科学,2015,44(2):157-160.
[15]侯珲,朱建兰,周红平,等.BTH和水杨酸(SA)对甜瓜抗白粉病的诱导作用[J].果树学报,2006,23(5):736-739.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[17]范文静,崔新仪,王学利.植物激活剂苯并噻二唑(BTH)的研究进展[J].天津农学院学报,2009,16(3):52-54.
[18]王文娟,赵建庄,魏朝俊,等.苯并噻二唑(BTH)对苹果抗斑点落叶病的诱导[J].果树学报,2008,25(3):362-366.