冬枣轮纹病菌拮抗放线菌的筛选、鉴定及其抑制效果
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摘要
旨在筛选出能高效抑制冬枣轮纹病病原真菌的放线菌,为冬枣轮纹病的生物防治提供理论依据。通过纯培养法从黄河三角洲贝壳堤岛贝沙沉积物中分离获得198株放线菌,对抑菌作用最强的菌株BK作了进一步研究。通过形态特征、生理生化特征和16S rRNA序列分析对菌株BK进行了鉴定,并研究了其对冬枣轮纹病菌抑制效果。结果表明,菌株BK对冬枣轮纹病菌丝生长抑制率达72.30%。菌株BK发酵液对离体冬枣果实轮纹病的防效为73.72%,与50%多菌灵的防效相差不显著。经鉴定菌株BK为绛红北里孢菌是一株较为理想的拮抗菌,在冬枣轮纹病生物防治方面有较好的应用前景。
The objective is to select actinomycete which has strong antagonistic ability against winter jujube ring grain disease. Total 198 actinomycetes were isolated by culture-dependent method from the shell sediment in the Seashell Islands of Yellow River Delta,and the strainBK with the strongest antagonistic ability was further studied. Strain BK was identified based on its morphology,physio-biochemical charactersand 16 S rRNA sequence analysis,and its ability against winter jujube ring grain disease was also studied,providing a theoretical basis inbiological control of winter jujube ring grain disease. The results showed that BK significantly inhibited the mycelium growth of Macrophoma kuwatsukai,with inhibitory rate of 72.3%. The control effect of the culture filtrate against winter jujube ring grain disease was 73.7%,whichhad no significant difference from that by the 50% carbendazim. Strain BK was identified as Streptomyces purpureus. To sum up,strain BK is anideal antagonistic one,and presents a potential application for the biological control of winter jujube ring grain disease.
The objective is to select actinomycete which has strong antagonistic ability against winter jujube ring grain disease. Total 198 actinomycetes were isolated by culture-dependent method from the shell sediment in the Seashell Islands of Yellow River Delta,and the strainBK with the strongest antagonistic ability was further studied. Strain BK was identified based on its morphology,physio-biochemical charactersand 16 S rRNA sequence analysis,and its ability against winter jujube ring grain disease was also studied,providing a theoretical basis inbiological control of winter jujube ring grain disease. The results showed that BK significantly inhibited the mycelium growth of Macrophoma kuwatsukai,with inhibitory rate of 72.3%. The control effect of the culture filtrate against winter jujube ring grain disease was 73.7%,whichhad no significant difference from that by the 50% carbendazim. Strain BK was identified as Streptomyces purpureus. To sum up,strain BK is anideal antagonistic one,and presents a potential application for the biological control of winter jujube ring grain disease.
引文
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[3]任迁琪,吴洪生,李季,等.西瓜枯萎病拮抗链霉菌分离鉴定及盆栽抗病试验[J].生物技术通报,2016,32(9):107-113.
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[13]田家怡,谢文军,孙景宽.黄河三角洲贝壳堤岛脆弱生态系统破坏现状及保护对策[J].环境科学与管理,2009,34(8):138-143.
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[15]阮继生,黄英.放线菌快速鉴定与系统分类[M].北京:科学出版社,2011,69-77.
[16]徐大勇,李志栋,李峰,等.番茄灰霉病菌拮抗放线菌的筛选、鉴定及其活性评价[J].中国生物防治学报,2012,28(2):298-302.
[17]陆继臣,迟乃玉,张庆芳.生防细菌CNY-04筛选、鉴定及其对灰霉病菌的防效[J].微生物学通报2013,40(9):1631-1637.
[18]杜廷芹,黄海军,王珍岩,等.黄河三角洲北部贝壳堤岛的近期演变[J].海洋地质与第四纪地质,2009,29(3):23-29.
[19]刘庆,孙景宽,田家怡,等.黄河三角洲贝壳堤岛典型建群植物养分吸收积累特征[J].水土保持研究,2010,17(3):153-156,161.
[20]赵丽萍,段代祥.黄河三角洲贝壳堤岛自然保护区维管植物区系研究[J].武汉植物学研究,2009,27(5):552-556.
[21]赵艳云,胡相明,刘京涛.贝壳堤地区微生物分布特征及其与植被分布的关系[J].水土保持通报,2012,32(2):267-270.
[2]靳雅君,张泽勇,冬枣轮纹病的发生与防治[J].北京农业,2006,9:31.
[3]任迁琪,吴洪生,李季,等.西瓜枯萎病拮抗链霉菌分离鉴定及盆栽抗病试验[J].生物技术通报,2016,32(9):107-113.
[4]姜怡,徐平,娄恺,徐丽华,等.放线菌药物资源开发面临的问题与对策[J].微生物学通报,2008,35(2):272-274.
[5]周启,王道本.农用抗生素和微生物杀虫剂[M].北京:农业出版社,1995.
[6]马海玲,刘俊华.褐黄水灰藓提取液对冬枣轮纹病菌的抑制效应[J].落叶果树,2014,46(5):13-15.
[7]刘俊华,张孝霖.黄牛毛藓水提液对冬枣轮纹病致病菌抑制效应的初步研究[J].安徽农学通报,2015,21(23):23-25.
[8]刘雪红,王宝琴.冬枣轮纹病病原真菌的分离及其拮抗菌的初筛.华北农学报[J].2014,29(1):227-231.
[9]Berdy J.Bioactive microbial metabolites,a personal review[J].Journal of Antibiotics,2005,58(4):1-26
[10]姜怡,曹艳茹,韩力,等.五种动物粪便纯培养放线菌的多样性及生物活性[J].微生物学报,2012,52(10):1282-1289.
[11]Jensen PR.Linking species concepts to natural product discoveryin the post-genomic era[J].Journal of Industrial Microbiologyand Biotechnology,2010,7:219-224.
[12]田守征,刘凯,徐丽华,等.嗜盐放线菌资源及次生代谢产物研究进展[J].微生物学通报,2014,41(9):1882-1890.
[13]田家怡,谢文军,孙景宽.黄河三角洲贝壳堤岛脆弱生态系统破坏现状及保护对策[J].环境科学与管理,2009,34(8):138-143.
[14]Shirling EB,Gottlieb D.Methods for characterization of Streptomyces species[J].International Journal of Systematic and Evolutionary Microbiology,1966,16(3):313-340.
[15]阮继生,黄英.放线菌快速鉴定与系统分类[M].北京:科学出版社,2011,69-77.
[16]徐大勇,李志栋,李峰,等.番茄灰霉病菌拮抗放线菌的筛选、鉴定及其活性评价[J].中国生物防治学报,2012,28(2):298-302.
[17]陆继臣,迟乃玉,张庆芳.生防细菌CNY-04筛选、鉴定及其对灰霉病菌的防效[J].微生物学通报2013,40(9):1631-1637.
[18]杜廷芹,黄海军,王珍岩,等.黄河三角洲北部贝壳堤岛的近期演变[J].海洋地质与第四纪地质,2009,29(3):23-29.
[19]刘庆,孙景宽,田家怡,等.黄河三角洲贝壳堤岛典型建群植物养分吸收积累特征[J].水土保持研究,2010,17(3):153-156,161.
[20]赵丽萍,段代祥.黄河三角洲贝壳堤岛自然保护区维管植物区系研究[J].武汉植物学研究,2009,27(5):552-556.
[21]赵艳云,胡相明,刘京涛.贝壳堤地区微生物分布特征及其与植被分布的关系[J].水土保持通报,2012,32(2):267-270.