三丁基锡暴露对雄性昆明小鼠生殖系统组织结构的影响
|
摘要
为了研究三丁基锡对雄性生殖系统的影响,试验以昆明小鼠为试验动物,经0,0.2,2,20μg/m L(分别为对照、低剂量、中剂量和高剂量组)的三丁基锡暴露45 d后,观察其对雄性小鼠睾丸、附睾、输精管等组织结构的影响。结果表明:三丁基锡暴露后,睾丸的组织结构发生明显改变,而且损伤程度随三丁基锡剂量的升高而加重。高剂量组生精细胞排列杂乱无序,各级生精细胞间出现间隙,睾丸间质没有显著变化。生精小管中精母细胞和精原细胞数目在各剂量组间无显著差异;而精子数目发生显著变化,低剂量组和对照组存在显著差异,中剂量组和对照组、低剂量组之间无显著差异,高剂量组和对照组、中剂量组存在显著差异。附睾管上皮细胞排列疏松,管间结缔组织减少、间隙变大,损伤程度随剂量升高而加剧,高剂量组最为严重,输精管形状发生变化,组织形态发生改变、腔壁脱落;中剂量组表现最明显,输精管的管状结构几乎完全消失。说明45 d的三丁基锡暴露对昆明小鼠雄性生殖系统的组织结构有影响且具剂量效应。
A study was conducted to research the reproductive toxicity of tributyltin( TBT) in male mammals,40 male mice were used as experimental subject treated with 0,0.2,2,20 μg/m L bodyweight dose of TBT. The histological structure of testis,epididymis,vas deferens were observed after 45-days of exposure. The results showed that the histological structure of testis significantly changed after exposure to TBT and the damage,which manifested dose dependent manner compared to the control. In 20 μg/m L group,spermatogenic cells arranged messily and disorderly,at the same time,gaps appeared among spermatogenic cell,interstitial tissue of testis displayed no significantly difference. The number of spermatogonia and spermatocyte manifested no significant change after TBT treatment; however,sperm cells displayed significant change between TBT treatment group and control,but no significant difference in other groups. After TBT exposure,the epithelium of duct of epididymis arranged loosely; the connective tissue reduced and the gap broadened between the ducts. The damage manifested dose dependently and reached the most serious in 20 μg/m L group. The shape and morphology of vas deferens was changed. In the middle concentration of TBT group,the deformity was the most obvious; the tubular of vas deferens was almost disappeared. It was concluded that TBT exposure changed the reproductive system of male Kunming mice with dose-dependent manner.
A study was conducted to research the reproductive toxicity of tributyltin( TBT) in male mammals,40 male mice were used as experimental subject treated with 0,0.2,2,20 μg/m L bodyweight dose of TBT. The histological structure of testis,epididymis,vas deferens were observed after 45-days of exposure. The results showed that the histological structure of testis significantly changed after exposure to TBT and the damage,which manifested dose dependent manner compared to the control. In 20 μg/m L group,spermatogenic cells arranged messily and disorderly,at the same time,gaps appeared among spermatogenic cell,interstitial tissue of testis displayed no significantly difference. The number of spermatogonia and spermatocyte manifested no significant change after TBT treatment; however,sperm cells displayed significant change between TBT treatment group and control,but no significant difference in other groups. After TBT exposure,the epithelium of duct of epididymis arranged loosely; the connective tissue reduced and the gap broadened between the ducts. The damage manifested dose dependently and reached the most serious in 20 μg/m L group. The shape and morphology of vas deferens was changed. In the middle concentration of TBT group,the deformity was the most obvious; the tubular of vas deferens was almost disappeared. It was concluded that TBT exposure changed the reproductive system of male Kunming mice with dose-dependent manner.
引文
[1]ZHANG J,ZHANG C,SUN P,et al.Tributyltin affects shoaling and anxiety behavior in female rareminnow(Gobiocypris rarus)[J].Aquat Toxicol,2016,178:80-87.
[2]江桂斌,徐福正,何滨,等.有机锡化合物测定方法研究进展[J].海洋环境科学,1999,18(3):61-68.
[3]袁玲玲,牛增元,叶曦雯,等.GC-MS法测定青岛海滨海水中的有机锡[J].海洋湖沼通报,2007(1):62-68.
[4]刘慧刚,徐立红.三丁基锡毒性作用生物标记研究进展[J].中华预防医学杂志,2005,39(4):288-289.
[5]吴宛青,张彬,姚子伟,等.渤海海域主要港口有机锡化合物监测分析[J].大连海事大学学报,2009,35(3):115-118.
[6]胡冠九,徐明华.有机锡化合物的性质、环境污染来源及测定方法[J].环境监测管理与技术,2000,12(S1):14-16,30.
[7]高俊敏,胡建英,郑泽根.海洋生物的有机锡化合物污染[J].海洋科学,2006,30(5):65-70.
[8]陆贤昆,李静,王臻,等.几种海洋微藻对水中三丁基锡化合物的迁移和转化作用[J].环境科学学报,1999,14(3):341-348.
[9]WHALEN M M,LOGANATHAN B G,KANNAN K.Immunotoxicity of environmentally relevant concentrations of butyltins on human natural killer cells in vitro[J].Environ Res,1999,81(2):108-116.
[10]WHALEN M M,LOGANATHAN B G.Butyltin exposure causes a rapid decrease in cyclic AMP levels in human lymphocytes[J].Toxicol Appl Pharmacol,2001,171(3):141-148.
[11]De SANTIAGE A,AGUILAR-SANTELISES M.Organotin compounds decrease in vitro survival,proliferation and differentiation of normal human B lymphocytes[J].Hum Exp Toxicol,1999,18(10):619-624.
[12]HARAZONO A,EMA M,OGAWA Y.Evaluation of early embryonic loss induced by tributyltin chloride in rats:phase and dose dependent antifertility effects[J].Arch Environ Contam Toxicol,1998,34(1):94-99.
[13]CHERNOFF N,SETZER R W,MILLER D B,et al.Effects of chemically induced maternal toxicity on prenatal development in the rat[J].Teratology,1990,42(6):651-658.
[14]EMA M,MIYAWAKI E,KAWASHIMA K.Development toxicology of TPTCL after edministration on three consecutive days during organagenesis in rats[J].Bull Environ Contam Taxicol,1999,62(2):363-370.
[15]张纪亮,熊建利,朱文文,等.三苯基锡对褐菖鮋精巢发育的影响及其作用机制[J].海洋渔业,2011,33(3):319-324.
[16]OMURA M,OGATA R,KUBO K,et al.Two-generation reproductive toxicity study of tributyltin chloride in male rats[J].Toxicol Sci,2001,64(2):224-232.
[17]王成恩,司纪亮,张森,等.孕期三丁基氯化锡染毒对子代雄性大鼠发育及性激素的影响[J].环境与健康杂志,2009,26(7):573-575.
[18]张森,司纪亮,王成恩,等.性别分化关键期三丁基氯化锡暴露对大鼠生精功能和睾丸酶活力的影响[J].环境与健康杂志,2009,26(8):691-693.
[19]LYSSIMACHOU A,SANTOS J G,ANDRA,et al.The mammalian“obesogen”tributyltin targets hepatic triglyceride accumulation and the transcriptional regulation of lipid metabolism in the liver and brain of Zebrafish[J].PLo S One,2015,10(12):e0143911.
[20]OMURA M,SHIMASAKI Y,OSHIMA Y,et al.Distribution of tributyltin,dibutyltin and monobutyltin in the liver,brain and fat of rats:Two-generation toxicity study of tributyltin chloride[J].Environ Sci,2004,11(2):123-132.
[21]鄢绯寰.三丁基锡对雄性小鼠精子发生和成熟的影响[D].厦门:厦门大学,2008.
[22]SUN L,ZHANG J,ZUO Z,et al.Influence of triphenyltin exposure on the hypothalamus-pituitary-gonad axis in male Sebastiscus marmoratus[J].Aquat Toxicol,2011,104(3/4):263-269.
[2]江桂斌,徐福正,何滨,等.有机锡化合物测定方法研究进展[J].海洋环境科学,1999,18(3):61-68.
[3]袁玲玲,牛增元,叶曦雯,等.GC-MS法测定青岛海滨海水中的有机锡[J].海洋湖沼通报,2007(1):62-68.
[4]刘慧刚,徐立红.三丁基锡毒性作用生物标记研究进展[J].中华预防医学杂志,2005,39(4):288-289.
[5]吴宛青,张彬,姚子伟,等.渤海海域主要港口有机锡化合物监测分析[J].大连海事大学学报,2009,35(3):115-118.
[6]胡冠九,徐明华.有机锡化合物的性质、环境污染来源及测定方法[J].环境监测管理与技术,2000,12(S1):14-16,30.
[7]高俊敏,胡建英,郑泽根.海洋生物的有机锡化合物污染[J].海洋科学,2006,30(5):65-70.
[8]陆贤昆,李静,王臻,等.几种海洋微藻对水中三丁基锡化合物的迁移和转化作用[J].环境科学学报,1999,14(3):341-348.
[9]WHALEN M M,LOGANATHAN B G,KANNAN K.Immunotoxicity of environmentally relevant concentrations of butyltins on human natural killer cells in vitro[J].Environ Res,1999,81(2):108-116.
[10]WHALEN M M,LOGANATHAN B G.Butyltin exposure causes a rapid decrease in cyclic AMP levels in human lymphocytes[J].Toxicol Appl Pharmacol,2001,171(3):141-148.
[11]De SANTIAGE A,AGUILAR-SANTELISES M.Organotin compounds decrease in vitro survival,proliferation and differentiation of normal human B lymphocytes[J].Hum Exp Toxicol,1999,18(10):619-624.
[12]HARAZONO A,EMA M,OGAWA Y.Evaluation of early embryonic loss induced by tributyltin chloride in rats:phase and dose dependent antifertility effects[J].Arch Environ Contam Toxicol,1998,34(1):94-99.
[13]CHERNOFF N,SETZER R W,MILLER D B,et al.Effects of chemically induced maternal toxicity on prenatal development in the rat[J].Teratology,1990,42(6):651-658.
[14]EMA M,MIYAWAKI E,KAWASHIMA K.Development toxicology of TPTCL after edministration on three consecutive days during organagenesis in rats[J].Bull Environ Contam Taxicol,1999,62(2):363-370.
[15]张纪亮,熊建利,朱文文,等.三苯基锡对褐菖鮋精巢发育的影响及其作用机制[J].海洋渔业,2011,33(3):319-324.
[16]OMURA M,OGATA R,KUBO K,et al.Two-generation reproductive toxicity study of tributyltin chloride in male rats[J].Toxicol Sci,2001,64(2):224-232.
[17]王成恩,司纪亮,张森,等.孕期三丁基氯化锡染毒对子代雄性大鼠发育及性激素的影响[J].环境与健康杂志,2009,26(7):573-575.
[18]张森,司纪亮,王成恩,等.性别分化关键期三丁基氯化锡暴露对大鼠生精功能和睾丸酶活力的影响[J].环境与健康杂志,2009,26(8):691-693.
[19]LYSSIMACHOU A,SANTOS J G,ANDRA,et al.The mammalian“obesogen”tributyltin targets hepatic triglyceride accumulation and the transcriptional regulation of lipid metabolism in the liver and brain of Zebrafish[J].PLo S One,2015,10(12):e0143911.
[20]OMURA M,SHIMASAKI Y,OSHIMA Y,et al.Distribution of tributyltin,dibutyltin and monobutyltin in the liver,brain and fat of rats:Two-generation toxicity study of tributyltin chloride[J].Environ Sci,2004,11(2):123-132.
[21]鄢绯寰.三丁基锡对雄性小鼠精子发生和成熟的影响[D].厦门:厦门大学,2008.
[22]SUN L,ZHANG J,ZUO Z,et al.Influence of triphenyltin exposure on the hypothalamus-pituitary-gonad axis in male Sebastiscus marmoratus[J].Aquat Toxicol,2011,104(3/4):263-269.