何首乌中二苯乙烯苷类物质的分离纯化及抗氧化活性研究
|
摘要
目的:研究何首乌中二苯乙烯苷类物质的分离纯化及抗氧化活性。方法:采用超声波辅助提取技术,从何首乌中提取得到二苯乙烯苷类物质,并通过NKA-9型大孔树脂对何首乌中二苯乙烯苷类物质进行分离纯化。将纯化后的二苯乙烯苷进行DPPH·和·OH的清除能力及Fe~(3+)的还原力测定。结果:NKA-9型大孔树脂对何首乌二苯乙烯苷的静态吸附率和解析率分别达到80.75%和92.36%,粗提物二苯乙烯苷纯度为34.62%,纯化后的二苯乙烯苷纯度为72.68%。纯化二苯乙烯苷对·OH和DPPH·的清除率分别达到72.72%和78.73%,还原力约为同浓度Vc的40%。结论:何首乌中二苯乙烯苷类物质表现出较好的抗氧化活性。
Objectives:The purification and antioxidant activity of stilbene glycosides from PolygonummultiflorumThunb were studied. Methods:The stilbene glycosides were extracted from PolygonummultiflorumThunb by ultrasonic extraction.The stilbene glycosides in PolygonummultiflorumThunb were separated and purified by NKA-9 macroporous resin. The antioxidant activities of the purified stilbene glycosides were evaluated through their capability of scavenging ·OH,DPPH· and the reduction ability to Fe~(3+).Results:The static adsorption and resolution rate of the NKA-9 macroporous resin for stilbene glycosides from PolygonummultiflorumThunb were 80.75% and 92.36%, respectively. The purity of the crude stilbene glycosides and the purified stilbene glycosides were 34.62% and 72.68%. The scavenging rates of purified stilbene glycosides against DPPH·and·OHwere 72.72% and 78.73%, respectively. The reductive power was about 40% of Vc of the same concentration. Conclusions:The stilbene glycosides in PolygonummultiflorumThunb showed good antioxidant activity.
Objectives:The purification and antioxidant activity of stilbene glycosides from PolygonummultiflorumThunb were studied. Methods:The stilbene glycosides were extracted from PolygonummultiflorumThunb by ultrasonic extraction.The stilbene glycosides in PolygonummultiflorumThunb were separated and purified by NKA-9 macroporous resin. The antioxidant activities of the purified stilbene glycosides were evaluated through their capability of scavenging ·OH,DPPH· and the reduction ability to Fe~(3+).Results:The static adsorption and resolution rate of the NKA-9 macroporous resin for stilbene glycosides from PolygonummultiflorumThunb were 80.75% and 92.36%, respectively. The purity of the crude stilbene glycosides and the purified stilbene glycosides were 34.62% and 72.68%. The scavenging rates of purified stilbene glycosides against DPPH·and·OHwere 72.72% and 78.73%, respectively. The reductive power was about 40% of Vc of the same concentration. Conclusions:The stilbene glycosides in PolygonummultiflorumThunb showed good antioxidant activity.
引文
[1]国家药典委员会,中国药典[M].一部.北京:中国医药科技出版社,2010.
[2]杨宇,万玉华,黄春静,等.HPLC法检测中药洗发水中何首乌二苯乙烯苷的含量[J].应用化工,2013,42(2):380-382.
[3]汪杰,曹骋.DM-130大孔吸附树脂纯化何首乌中二苯乙烯苷的工艺研究[J].中国现代中药,2013,15(7):591-593.
[4]杨晓丽,王立为.中药何首乌的药理作用研究进展[J].中医药信息,2004,21(6):12-14.
[5]廖海民,胡正海.何首乌的生物学及化学成分研究进展[J].中草药,2005,36(2):311-314.
[6]Zhang L,Xing Y,Ye C F,et al.Learning-memory Deficit with Aging in APP Transgenic Mice of Alzheimer's Disease and Intervention by using Tetrahydroxystilbene Glucoside[J].Behavioural Brain Research,2006,173(2):246-254.
[7]王文静,薛咏梅,赵荣华,等.何首乌的化学成分和药理作用研究进展[J].云南中医学院学报,2007,30(3):60-64.
[8]廖海民,王玲丽,谭玲玲,等.不同产地何首乌中二苯乙烯苷的测定[J].中草药,2006,37(4):603-604.
[9]桂本,郭嘉,池汝安,等.大孔吸附树脂对葛根总黄酮吸附性能的研究[J].应用化工,2007,36(5):451-453.
[10]Guo J,Yang X W.Studies on Triterpenoid Saponins of Seeds ofAesculuschinensis Bungevar.chekiangensis(Hu et Fang)Fang[J].Journal of Chinese Pharmaceutical Sciences,2004,13(2):87-91.
[11]邓祥,黄小梅,吴狄.何首乌中二苯乙烯苷类物质的超声波提取工艺优化[J].应用化工,2015,44(3):453-455.
[12]涂宗财,刘光宪,豆玉新,等.苦槠果实总黄酮的提取与纯化[J].食品研究与开发,2008,29(1):33-36.
[13]翟梅枝,郭琪,贾彩霞,等.大孔树脂分离纯化核桃青皮总黄酮的研究[J].生物质化学工程,2008,42(3):21-25.
[14]吕晓玲,邱松山,孙晓侠,等.油茶总皂苷的抗氧化及清除自由基能力初步研究[J].食品科学,2005,26(11):86-90.
[15]陈晴晴,杨金凤,刘红.新疆沙枣多糖的提取分离及抗氧化活性研究[J].食品研究与开发,2016,37(12):37-40.
[2]杨宇,万玉华,黄春静,等.HPLC法检测中药洗发水中何首乌二苯乙烯苷的含量[J].应用化工,2013,42(2):380-382.
[3]汪杰,曹骋.DM-130大孔吸附树脂纯化何首乌中二苯乙烯苷的工艺研究[J].中国现代中药,2013,15(7):591-593.
[4]杨晓丽,王立为.中药何首乌的药理作用研究进展[J].中医药信息,2004,21(6):12-14.
[5]廖海民,胡正海.何首乌的生物学及化学成分研究进展[J].中草药,2005,36(2):311-314.
[6]Zhang L,Xing Y,Ye C F,et al.Learning-memory Deficit with Aging in APP Transgenic Mice of Alzheimer's Disease and Intervention by using Tetrahydroxystilbene Glucoside[J].Behavioural Brain Research,2006,173(2):246-254.
[7]王文静,薛咏梅,赵荣华,等.何首乌的化学成分和药理作用研究进展[J].云南中医学院学报,2007,30(3):60-64.
[8]廖海民,王玲丽,谭玲玲,等.不同产地何首乌中二苯乙烯苷的测定[J].中草药,2006,37(4):603-604.
[9]桂本,郭嘉,池汝安,等.大孔吸附树脂对葛根总黄酮吸附性能的研究[J].应用化工,2007,36(5):451-453.
[10]Guo J,Yang X W.Studies on Triterpenoid Saponins of Seeds ofAesculuschinensis Bungevar.chekiangensis(Hu et Fang)Fang[J].Journal of Chinese Pharmaceutical Sciences,2004,13(2):87-91.
[11]邓祥,黄小梅,吴狄.何首乌中二苯乙烯苷类物质的超声波提取工艺优化[J].应用化工,2015,44(3):453-455.
[12]涂宗财,刘光宪,豆玉新,等.苦槠果实总黄酮的提取与纯化[J].食品研究与开发,2008,29(1):33-36.
[13]翟梅枝,郭琪,贾彩霞,等.大孔树脂分离纯化核桃青皮总黄酮的研究[J].生物质化学工程,2008,42(3):21-25.
[14]吕晓玲,邱松山,孙晓侠,等.油茶总皂苷的抗氧化及清除自由基能力初步研究[J].食品科学,2005,26(11):86-90.
[15]陈晴晴,杨金凤,刘红.新疆沙枣多糖的提取分离及抗氧化活性研究[J].食品研究与开发,2016,37(12):37-40.