急性心肌梗死患者肠道菌群的变化
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摘要
目的探究急性心肌梗死患者肠道菌群的变化。方法选择陕西省核工业二一五医院2017年1月至2018年9月收治的100例急性心肌梗死患者纳入研究组,同期于陕西省核工业二一五医院行冠状动脉造影检测排除冠心病的100例住院患者纳入对照组;采集患者大便及血清标本,采用实时荧光定量聚合酶链反应(qPCR)检测两组患者肠道优势菌群的差异,同时测定研究组患者的血清脑钠肽前体及肌钙蛋白水平,并与不同肠道菌群进行相关分析。结果与对照组患者相比,研究组患者的优势菌群明显改变,肠杆菌及肠球菌占优势,且研究组患者这些细菌水平与血清脑钠肽前体及肌钙蛋白水平呈正相关,而双歧杆菌属、乳酸杆菌属水平与血清脑钠肽前体及肌钙蛋白水平呈负相关。结论急性心肌梗死患者的肠道优势菌群发生改变,优势菌群分布与心肌梗死患者的血清脑钠肽前体及肌钙蛋白水平存在相关性。
Objective To explore the changes in intestinal flora diversity in patients with acute myocardial infarction.Methods A total 100 patients with acute myocardial infarction admitted to No.215 Hospital of Shaanxi Nuclear Industry from January 2017 to September 2018 were selected into study group,other 100 hospitalized patients who underwent coronary angiography to exclude coronary heart disease were selected into control group.The patients′stool and serum samples were collected.The difference of intestinal dominant flora between the two groups was detected by real-time quantitative polymerase chain reaction.The serum brain natriuretic peptide and troponin levels of the study group were determined and compared,correlation analysis was performed on intestinal flora.Results Compared with the control group,the dominant flora of the study group changed significantly,and Enterobacter and Enterococcus were dominant which levels were significant higher.The number of these bacteria positively related with serum pro-brain natriuretic peptide and troponin levels.The Bifidobacteria and Lactobacillas levels negatively correlated with serum brain natriuretic peptide precursor and troponin levels.Conclusion In patients with acute myocardial infarction,the dominant flora changed.The distribution of dominant flora related to serum pro-brain natriuretic peptide and troponin levels of myocardial infarction patients.
Objective To explore the changes in intestinal flora diversity in patients with acute myocardial infarction.Methods A total 100 patients with acute myocardial infarction admitted to No.215 Hospital of Shaanxi Nuclear Industry from January 2017 to September 2018 were selected into study group,other 100 hospitalized patients who underwent coronary angiography to exclude coronary heart disease were selected into control group.The patients′stool and serum samples were collected.The difference of intestinal dominant flora between the two groups was detected by real-time quantitative polymerase chain reaction.The serum brain natriuretic peptide and troponin levels of the study group were determined and compared,correlation analysis was performed on intestinal flora.Results Compared with the control group,the dominant flora of the study group changed significantly,and Enterobacter and Enterococcus were dominant which levels were significant higher.The number of these bacteria positively related with serum pro-brain natriuretic peptide and troponin levels.The Bifidobacteria and Lactobacillas levels negatively correlated with serum brain natriuretic peptide precursor and troponin levels.Conclusion In patients with acute myocardial infarction,the dominant flora changed.The distribution of dominant flora related to serum pro-brain natriuretic peptide and troponin levels of myocardial infarction patients.
引文
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[13]BRUGERE J F,BORREL G,GACI N,et al.Archaebiotics:proposed therapeutic use of archaea to prevent trimethylaminuria and cardiovascular disease[J].Gut Microbes,2014,5(1):5-10.
[14]周丹,黄雨晴,余雪菊,等.肠道菌群与心血管病关系的研究进展[J].中华高血压杂志,2018,26(5):417-423.
[2]LAM V,SU J,HSU A,et al.Intestinal microbial metabolites are linked to severity of myocardial infarction in rats[J].PLoS One,2016,11(8):e0160840.
[3]ZHU W,GREGORY J C,ORG E,et al.Gut Microbial metabolite TMAO enhances platelet hyperreactivity and thrombosis risk[J].Cell,2016,165(1):111-124.
[4]中华医学会心血管病学分会,中华心血管病杂志编辑委员会.急性ST段抬高型心肌梗死诊断和治疗指南[J].中华心血管病杂志,2010,38(8):675-690.
[5]王丽君,谢莲娜,周雪,等.急性心肌梗死患者肠道菌群多样性分析[J].中国微生态学杂志,2017,29(10):1164-1167.
[6]DINAKARAN V,RATHINAVEL A,PUSHPANATHANM,et al.Elevated levels of circulating DNA in cardiovascular disease patients:metagenomic profiling of microbiome in the circulation[J].PLoS One,2014,9(8):e105221.
[7]黄莹,谭超超.急性心肌梗死患者肠道优势菌群分析[J].中国微生态学杂志,2014,26(9):1004-1008.
[8]WU Z W,LING Z X,LU H F,et al.Changes of gut bacteria and immune parameters in liver transplant recipients[J].Hepatobiliary Pancreat Dis Int,2016,11(1):40-50.
[9]金华,金钊,张蕾蕾.肠道菌群可能为高血压发病的环境因素[J].中国微生态学杂志,2015,27(1):121-125.
[10]解傲,袁杰利.肠道菌群与代谢性疾病[J].胃肠病学和肝病学杂志,2013,22(2):102-104.
[11]EMOTO T,YAMASHITA T,KOBAYASHI T,et al.Characterization of gut microbiota profiles in coronary artery disease patients using data mining analysis of terminal restriction fragment length polymorphism:gut microbiota could be a diagnostic marker of coronary artery disease[J].Heart Vessels,2017,32(1):39-46.
[12]王玲,李群.冠心病患者肠道菌群分布及其与尿酸代谢的关系分析[J].现代消化及介入诊疗,2015,17(6):327-330.
[13]BRUGERE J F,BORREL G,GACI N,et al.Archaebiotics:proposed therapeutic use of archaea to prevent trimethylaminuria and cardiovascular disease[J].Gut Microbes,2014,5(1):5-10.
[14]周丹,黄雨晴,余雪菊,等.肠道菌群与心血管病关系的研究进展[J].中华高血压杂志,2018,26(5):417-423.