三个多倍体萱草品种的组培快繁
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摘要
以多倍体萱草三个品种(代号分别为28、41、42)的花茎及幼蕾为外植体进行组培研究,结果表明,花茎的愈伤诱导及其相应的芽分化明显好于花蕾,花茎做外植体更适宜;以MS+(2.0～2.5) mg/L BA+(0.20～0.25) mg/L NAA培养基的愈伤诱导效果较好,三个品种中以41号愈伤形成早且量大,28号次之,42号愈伤出现较晚且量较少。在诱导芽分化时,41号和28号在MS+2.5 mg/L BA+(0.20～0.25) mg/L NAA培养基上芽分化数量多,41号最易芽分化,28号次之;42号需要多代的芽诱导驯化培养才分化出芽,在MS+(2.5～3.0) mg/L BA+(0.25～0.30) mg/L NAA培养基上分化数量较多,但分化率低于41号和28号。三个品种在MS+2.5 mg/L BA+0.25 mg/L NAA培养基上的增殖效果较好,在1/2MS+0.10 mg/L NAA中适宜生根培养。
Three cultivars of polyploid Hemerocallis fulva(cultivar number 28, 41 and 42) were selected for tissue culture by using their floral axis and flower buds as explants. The results were as follows: the callus produced from floral axis were much more and the buds were more easily differentiated compared with flower bud, so the floral axis was preferably as explants. The culture media of MS+(2.0～2.5) mg/L BA+(0.20～0.25) mg/L NAA were better for callus induction. The callus induced from cultivar 41 was the earliest and the most followed by the cultivar 28, while that from the cultivar 42 was the latest and least. In inducing buds, the media of MS+2.5 mg/L BA+(0.20～0.25) mg/L NAA induced much more buds from the cultivars 41 and 28,in which, it was more easily to induce buds from the cultivar 41 compared with 28. It needed for many generations of domestication culture to induce buds for the cultivar 42, and the media of MS+(2.5～3.0) mg/L BA+(0.25～0.30) mg/L NAA was optimal, but its inducing rate was lower than that of the cultivars 41 and 28. The medium of MS+2.5 mg/L BA+0.25 mg/L NAA was better for multiplication culture, and the medium 1/2 MS+0.10 mg/L NAA was appropriate for rooting culture.
Three cultivars of polyploid Hemerocallis fulva(cultivar number 28, 41 and 42) were selected for tissue culture by using their floral axis and flower buds as explants. The results were as follows: the callus produced from floral axis were much more and the buds were more easily differentiated compared with flower bud, so the floral axis was preferably as explants. The culture media of MS+(2.0～2.5) mg/L BA+(0.20～0.25) mg/L NAA were better for callus induction. The callus induced from cultivar 41 was the earliest and the most followed by the cultivar 28, while that from the cultivar 42 was the latest and least. In inducing buds, the media of MS+2.5 mg/L BA+(0.20～0.25) mg/L NAA induced much more buds from the cultivars 41 and 28,in which, it was more easily to induce buds from the cultivar 41 compared with 28. It needed for many generations of domestication culture to induce buds for the cultivar 42, and the media of MS+(2.5～3.0) mg/L BA+(0.25～0.30) mg/L NAA was optimal, but its inducing rate was lower than that of the cultivars 41 and 28. The medium of MS+2.5 mg/L BA+0.25 mg/L NAA was better for multiplication culture, and the medium 1/2 MS+0.10 mg/L NAA was appropriate for rooting culture.
引文
[1] 柏文琴,闫桂琴,李瑞艳,等. 大花萱草不同外植体初代培养的比较研究[J]. 湖北大学学报(自然科学版),2012,34(1):89-92.
[2] 尹立辉,武术杰,刘亚亮,等. 多倍体萱草的离体快繁技术[J].东北林业大学学报, 2016, 44(8):41-43.
[3] 毕晓颖,王宁. 萱草花茎离体培养与快速繁殖[J].东北林业大学学报, 2012,40(11):56-59,158.
[4] 彭广霖,姜宁,潘仕梅,等. 大花萱草的花梗组织培养与快速繁殖[J].安徽农业科学,2012, 40(17): 9203-9205.
[5] 袁爱民,佟宝君,郝砚英,等. 多倍体萱草的组织培养与快速繁殖技术[J].天津农业科学, 2008, 14(3): 45- 47.
[6] 张洁茹,刘晓嘉,陈丽飞,等. 萱草优良单株花茎离体快繁[J].东北林业大学学报, 2014, 42(2): 73-77.
[7] 姜凤英,栾绍武,吴志刚,等. 多倍体萱草“金娃娃”的离体培养研究[J].辽宁农业科学, 2007(1): 51-52.
[8] 宋雪莲,董然,赵和祥, 等. 五种新品种多倍体萱草离体快繁技术研究[J].北方园艺, 2011(7): 127-129.
[2] 尹立辉,武术杰,刘亚亮,等. 多倍体萱草的离体快繁技术[J].东北林业大学学报, 2016, 44(8):41-43.
[3] 毕晓颖,王宁. 萱草花茎离体培养与快速繁殖[J].东北林业大学学报, 2012,40(11):56-59,158.
[4] 彭广霖,姜宁,潘仕梅,等. 大花萱草的花梗组织培养与快速繁殖[J].安徽农业科学,2012, 40(17): 9203-9205.
[5] 袁爱民,佟宝君,郝砚英,等. 多倍体萱草的组织培养与快速繁殖技术[J].天津农业科学, 2008, 14(3): 45- 47.
[6] 张洁茹,刘晓嘉,陈丽飞,等. 萱草优良单株花茎离体快繁[J].东北林业大学学报, 2014, 42(2): 73-77.
[7] 姜凤英,栾绍武,吴志刚,等. 多倍体萱草“金娃娃”的离体培养研究[J].辽宁农业科学, 2007(1): 51-52.
[8] 宋雪莲,董然,赵和祥, 等. 五种新品种多倍体萱草离体快繁技术研究[J].北方园艺, 2011(7): 127-129.